Restriction map for
pFN21AA0737
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlpI Bpu1102I BsaAI BsaHI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KpnI KspI MaeII MluI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 572 TCCGGA T^CCGGA
AvaI 1190 CCCGAG C^[CT]CG[GA]G
AvrII 468 CCTAGG C^CTAGG
BalI 168 TGGCCA TGG^CCA
BamHI 915 GGATCC G^GATCC
BbsI 594 GAAGAC GAAGAC(2/6)
BcnI 10 CCCGG CC^[GC]GG
BlnI 468 CCTAGG C^CTAGG
BpuAI 594 GAAGAC GAAGAC(2/6)
BsaBI 133 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1682 GGTCTC GGTCTC(1/5)
BsaWI 572 TCCGGA [AT]^CCGG[AT]
BseAI 572 TCCGGA T^CCGGA
BsoBI 1190 CCCGAG C^[CT]CG[GA]G
BspEI 572 TCCGGA T^CCGGA
BspMI 492 ACCTGC ACCTGC(4/8)
BsrBI 1549 GAGCGG CCGCTC(-3/-3)
BsrDI 1778 GCAATG GCAATG(2/0)
BssSI 550 CACGAG CACGAG(-5/-1)
BstBI 1439 TTCGAA TT^CGAA
BstEII 1182 GGTGACC G^GT[ACGT]ACC
BstPI 1182 GGTGACC G^GT[ACGT]ACC
BstXI 314 CCATGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1272 GCAGGC GC[ACGT]^[ACGT]GC
EaeI 168 TGGCCA [CT]^GGCC[GA]
EcoO65I 1182 GGTGACC G^GT[ACGT]ACC
FauI 287 GCGGG CCCGC(4/6)
HindIII 90 AAGCTT A^AGCTT
HpaI 1027 GTTAAC GTT^AAC
MamI 133 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 368 CAATTG C^AATTG
MluNI 168 TGGCCA TGG^CCA
MroI 572 TCCGGA T^CCGGA
MscI 168 TGGCCA TGG^CCA
MseI 1028 TTAA T^TAA
MunI 368 CAATTG C^AATTG
NciI 10 CCCGG CC^[GC]GG
NdeI 686 CATATG CA^TATG
NspI 248 ACATGC [GA]CATG^[CT]
NspV 1439 TTCGAA TT^CGAA
SfuI 1439 TTCGAA TT^CGAA
SmlI 1145 CTCAAG C^T[CT][GA]AG
SpeI 538 ACTAGT A^CTAGT
Tru9I 1028 TTAA T^TAA
XbaI 1831 TCTAGA T^CTAGA
XcmI 1059 CCAGGCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 287 GCGG CCGC(-3/-1)
1551 GCGG
AcsI 106 GAATTT [GA]^AATT[CT]
1836 AAATTC
AfaI 376 GTAC GT^AC
1217 GTAC
AlwNI 1280 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1283 CAGCTGCTG
AocI 1570 CCTGAGG CC^T[ACGT]AGG
1636 CCTGAGG
ApoI 106 GAATTT [GA]^AATT[CT]
1836 AAATTC
AvaII 234 GGTCC G^G[AT]CC
324 GGACC
BanI 478 GGCACC G^G[CT][GA]CC
1590 GGCACC
BclI 432 TGATCA T^GATCA
1619 TGATCA
BstYI 654 AGATCC [GA]^GATC[CT]
915 GGATCC
Bsu36I 1570 CCTGAGG CC^T[ACGT]AGG
1636 CCTGAGG
DraII 42 AGGGCCT [GA]G^G[ACGT]CC[CT]
629 AGGCCCC
DsaI 267 CCATGG C^C[GA][CT]GG
314 CCATGG
Eco81I 1570 CCTGAGG CC^T[ACGT]AGG
1636 CCTGAGG
EcoO109I 42 AGGGCCT [GA]G^G[ACGT]CC[CT]
629 AGGCCCC
FbaI 432 TGATCA T^GATCA
1619 TGATCA
HapII 11 CCGG C^CGG
573 CCGG
HpaII 11 CCGG C^CGG
573 CCGG
MflI 654 AGATCC [GA]^GATC[CT]
915 GGATCC
MspA1I 454 CAGCTG C[AC]G^C[GT]G
1283 CAGCTG
MspI 11 CCGG C^CGG
573 CCGG
NcoI 267 CCATGG C^CATGG
314 CCATGG
PflMI 237 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCATACAGTGG
PleI 1365 GACTC GAGTC(4/5)
1645 GAGTC
PvuII 454 CAGCTG CAG^CTG
1283 CAGCTG
RsaI 376 GTAC GT^AC
1217 GTAC
TaqI 1440 TCGA T^CGA
1710 TCGA
TthHB8I 1440 TCGA T^CGA
1710 TCGA
Van91I 237 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1578 CCATACAGTGG
XhoII 654 AGATCC [GA]^GATC[CT]
915 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AluI AlwI BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI Tsp45I
Tsp509I TspRI