(Circular) (Six-base) MAPSORT of: pFN21AA0737.seq Check: 3912 from: 1 to: 6568 pFN21AA0737 6568 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2506 2506 Size: 6568 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3911 1937 Size: 1974 4594 Acc65I G'GTAC_C Cuts at: 3889 3889 Size: 6568 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2532 2876 4631 6015 6283 1632 Size: 900 344 1755 1384 268 1917 Fragments arranged by size: 1917 1755 1384 900 344 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6371 AflIII A'CryG_T Cuts at: 1203 5467 6444 1203 Size: 4264 977 1327 Fragments arranged by size: 4264 1327 977 AgeI A'CCGG_T Cuts at: 5399 5399 Size: 6568 AhdI GACnn_n'nnGTC Cuts at: 5297 5297 Size: 6568 AleI CACnn'nnGTG Cuts at: 1166 3185 3854 1166 Size: 2019 669 3880 Fragments arranged by size: 3880 2019 669 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2507 1184 Size: 1323 5245 AlwNI CAG_nnn'CTG Cuts at: 3290 3293 4311 5883 3290 Size: 3 1018 1572 3975 Fragments arranged by size: 3975 1572 1018 3 ApaLI G'TGCA_C Cuts at: 4625 5781 4625 Size: 1156 5412 ApoI r'AATT_y Cuts at: 1493 2111 3841 3877 4103 4255 6364 1493 Size: 618 1730 36 226 152 2109 1697 Fragments arranged by size: 2109 1730 1697 618 226 152 36 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 3195 3893 1870 Size: 73 1252 698 4545 Fragments arranged by size: 4545 1252 698 73 AvrII C'CTAG_G Cuts at: 2473 2473 Size: 6568 BamHI G'GATC_C Cuts at: 2920 3898 4265 2920 Size: 978 367 5223 Fragments arranged by size: 5223 978 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2483 3595 3889 5344 618 Size: 325 217 627 696 1112 294 1455 1842 Fragments arranged by size: 1842 1455 1112 696 627 325 294 217 BanII G_rGCy'C Cuts at: 1391 2301 3006 3204 3887 1391 Size: 910 705 198 683 4072 Fragments arranged by size: 4072 910 705 683 198 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2606 928 Size: 971 707 4890 Fragments arranged by size: 4890 971 707 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4759 4793 1903 Size: 34 2822 34 3678 Fragments arranged by size: 3678 2822 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4460 5670 4460 Size: 1210 5358 BclI T'GATC_A Cuts at: 1548 2437 3624 1548 Size: 889 1187 4492 Fragments arranged by size: 4492 1187 889 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5179 136 Size: 107 122 71 631 605 3507 1525 Fragments arranged by size: 3507 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6563 6563 Size: 6568 BlpI GC'TnA_GC Cuts at: 3985 3985 Size: 6568 Bme1580I G_kGCm'C Cuts at: 1790 4629 5785 1790 Size: 2839 1156 2573 Fragments arranged by size: 2839 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6568 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2224 2497 3234 Size: 416 53 83 399 24 807 273 737 Cuts at: 3234 3772 4299 5257 442 Size: 538 527 958 1753 Fragments arranged by size: 1753 958 807 737 538 527 416 399 273 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6568 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3510 3705 3510 Size: 195 6373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2967 3096 3395 5228 1239 Size: 1728 129 299 1833 2579 Fragments arranged by size: 2579 1833 1728 299 129 Bpu10I CC'TnA_GC Cuts at: 3552 3552 Size: 6568 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3135 4693 5558 5856 3135 Size: 1558 865 298 3847 Fragments arranged by size: 3847 1558 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3693 5231 882 Size: 617 2194 1538 2219 Fragments arranged by size: 2219 2194 1538 617 BsaAI yAC'GTr Cuts at: 493 1204 4359 493 Size: 711 3155 2702 Fragments arranged by size: 3155 2702 711 BsaBI GATnn'nnATC Cuts at: 2142 6562 2142 Size: 4420 2148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4375 4757 5392 275 Size: 53 83 186 936 2842 382 635 1451 Fragments arranged by size: 2842 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2577 5001 5399 5673 5820 6231 2577 Size: 2424 398 274 147 411 2914 Fragments arranged by size: 2914 2424 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2932 2962 3147 3177 1178 Size: 30 165 30 1529 30 185 30 4569 Fragments arranged by size: 4569 1529 185 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2816 3393 3459 3462 1186 Size: 1630 577 66 3 4292 Fragments arranged by size: 4292 1630 577 66 3 BseYI C'CCAG_C Cuts at: 2457 2461 2591 2974 3080 3175 3708 5771 6267 Size: 4 130 383 106 95 533 2063 496 Cuts at: 6267 2457 Size: 2758 Fragments arranged by size: 2758 2063 533 496 383 130 106 95 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5995 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4018 4779 5807 1540 Size: 275 186 2017 761 1028 2301 Fragments arranged by size: 2301 2017 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4629 4714 5785 4629 Size: 85 1071 5412 Fragments arranged by size: 5412 1071 85 BsmI GAATG_Cn' Cuts at: 4079 4172 4079 Size: 93 6475 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 3006 3204 3887 4629 4714 5785 Size: 399 511 705 198 683 742 85 1071 Cuts at: 5785 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 705 683 511 399 198 85 BspEI T'CCGG_A Cuts at: 2577 6231 2577 Size: 3654 2914 BspHI T'CATG_A Cuts at: 4457 6187 4457 Size: 1730 4838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2506 3923 844 Size: 1662 1417 3489 Fragments arranged by size: 3489 1662 1417 BsrBI CCG'CTC Cuts at: 1394 1823 3556 4455 1394 Size: 429 1733 899 3507 Fragments arranged by size: 3507 1733 899 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3790 5063 5237 65 Size: 1183 2542 1273 174 1396 Fragments arranged by size: 2542 1396 1273 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5212 5399 1700 Size: 110 3402 187 2869 Fragments arranged by size: 3402 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6568 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6568 BssSI C'ACGA_G Cuts at: 2555 4628 5640 2555 Size: 2073 1012 3483 Fragments arranged by size: 3483 2073 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2867 3290 3293 794 Size: 2073 423 3 4069 Fragments arranged by size: 4069 2073 423 3 BstBI TT'CG_AA Cuts at: 3445 5372 3445 Size: 1927 4641 BstEII G'GTnAC_C Cuts at: 1184 3187 1184 Size: 2003 4565 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2326 Size: 6568 BstYI r'GATC_y Cuts at: 1972 2659 2920 3898 4265 4650 4667 6119 6205 Size: 687 261 978 367 385 17 1452 86 Cuts at: 6205 6217 6563 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 978 687 385 367 346 261 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3576 3642 3576 Size: 66 6502 BtgI C'CryG_G Cuts at: 513 1065 2004 2272 2319 513 Size: 552 939 268 47 4762 Fragments arranged by size: 4762 939 552 268 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5829 Fragments arranged by size: 5829 683 56 BtsI GCAGTG_nn' Cuts at: 3802 4084 4878 4898 3802 Size: 282 794 20 5472 Fragments arranged by size: 5472 794 282 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2754 2789 3834 3869 529 Size: 35 2190 35 1045 35 3228 Fragments arranged by size: 3228 2190 1045 35 35 35 DraI TTT'AAA Cuts at: 3872 3872 Size: 6568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5575 809 Size: 507 4259 1802 Fragments arranged by size: 4259 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2173 4015 4904 Size: 54 1425 50 275 18 343 1842 889 Cuts at: 4904 6533 8 Size: 1629 43 Fragments arranged by size: 1842 1629 1425 889 343 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4015 1537 Size: 275 2203 4090 Fragments arranged by size: 4090 2203 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2209 2639 3139 4498 1353 Size: 856 430 500 1359 3423 Fragments arranged by size: 3423 1359 856 500 430 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5155 5529 5675 387 Size: 1064 138 3566 374 146 1280 Fragments arranged by size: 3566 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2532 2876 2967 3096 3395 4631 5228 Size: 393 900 344 91 129 299 1236 597 Cuts at: 5228 6015 6283 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1236 900 787 597 393 344 299 268 129 91 EcoO109I rG'GnC_Cy Cuts at: 2048 2635 2048 Size: 587 5981 EcoRI G'AATT_C Cuts at: 3877 4255 3877 Size: 378 6190 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2537 2537 Size: 6568 FspI TGC'GCA Cuts at: 5074 5074 Size: 6568 HaeII r_GCGC'y Cuts at: 1678 5715 1678 Size: 4037 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2384 2416 2963 Size: 32 135 32 614 32 166 32 547 Cuts at: 2963 2995 3247 3279 3635 3667 5209 5241 5283 Size: 32 252 32 356 32 1542 32 42 Cuts at: 5283 5315 1373 Size: 32 2626 Fragments arranged by size: 2626 1542 614 547 356 252 166 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2427 2971 3034 3245 3912 4158 Size: 988 281 489 544 63 211 667 246 Cuts at: 4158 669 Size: 3079 Fragments arranged by size: 3079 988 667 544 489 281 246 211 63 HindIII A'AGCT_T Cuts at: 748 2095 748 Size: 1347 5221 HpaI GTT'AAC Cuts at: 3034 4158 3034 Size: 1124 5444 KpnI G_GTAC'C Cuts at: 3893 3893 Size: 6568 MfeI C'AATT_G Cuts at: 2373 4167 2373 Size: 1794 4774 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3229 5682 5866 1305 Size: 1924 2453 184 2007 Fragments arranged by size: 2453 2007 1924 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2175 10 Size: 54 1425 343 343 4403 Fragments arranged by size: 4403 1425 343 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2181 2258 2559 3185 3854 4526 4885 Size: 648 1015 77 301 626 669 672 359 Cuts at: 4885 5044 518 Size: 159 2042 Fragments arranged by size: 2042 1015 672 669 648 626 359 301 159 77 MspA1I CmG'CkG Cuts at: 1606 2461 3290 3984 4661 5809 6054 1606 Size: 855 829 694 677 1148 245 2120 Fragments arranged by size: 2120 1148 855 829 694 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2272 2319 513 Size: 552 939 268 47 4762 Fragments arranged by size: 4762 939 552 268 47 NdeI CA'TA_TG Cuts at: 387 2692 387 Size: 2305 4263 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6568 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NspI r_CATG'y Cuts at: 1132 1229 2257 3926 5471 1132 Size: 97 1028 1669 1545 2229 Fragments arranged by size: 2229 1669 1545 1028 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6568 PciI A'CATG_T Cuts at: 5467 5467 Size: 6568 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 3589 1108 Size: 1140 1341 4087 Fragments arranged by size: 4087 1341 1140 PfoI T'CCnGG_A Cuts at: 2014 2014 Size: 6568 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4637 4669 6150 6182 4637 Size: 32 1481 32 5023 Fragments arranged by size: 5023 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6568 PsiI TTA'TAA Cuts at: 4138 4138 Size: 6568 PstI C_TGCA'G Cuts at: 830 3396 3480 3782 3809 3920 830 Size: 2566 84 302 27 111 3478 Fragments arranged by size: 3478 2566 302 111 84 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6568 PvuII CAG'CTG Cuts at: 2461 3290 2461 Size: 829 5739 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SbfI CC_TGCA'GG Cuts at: 3920 3920 Size: 6568 ScaI AGT'ACT Cuts at: 4816 6528 4816 Size: 1712 4856 SfcI C'TryA_G Cuts at: 826 1045 2337 3332 3392 3476 3778 3805 3916 Size: 219 1292 995 60 84 302 27 111 Cuts at: 3916 5051 5732 5923 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1292 1135 995 681 302 219 191 111 84 60 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 3895 1872 Size: 2023 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3150 4672 5573 5835 820 Size: 197 926 1207 1522 901 262 1553 Fragments arranged by size: 1553 1522 1207 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4359 493 Size: 3866 2702 SpeI A'CTAG_T Cuts at: 152 2543 152 Size: 2391 4177 SphI G_CATG'C Cuts at: 1132 3926 1132 Size: 2794 3774 SspI AAT'ATT Cuts at: 5 52 4492 5 Size: 47 4440 2081 Fragments arranged by size: 4440 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6568 StyI C'CwwG_G Cuts at: 513 1065 2004 2133 2272 2319 2473 4007 513 Size: 552 939 129 139 47 154 1534 3074 Fragments arranged by size: 3074 1534 939 552 154 139 129 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2090 3255 4595 4612 4765 4950 5395 Size: 1255 510 1165 1340 17 153 185 445 Cuts at: 5395 325 Size: 1498 Fragments arranged by size: 1498 1340 1255 1165 510 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3221 4814 6526 Size: 274 80 33 51 1443 1244 1593 1712 Cuts at: 6526 96 Size: 138 Fragments arranged by size: 1712 1593 1443 1244 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6528 6560 6528 Size: 32 6536 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6568 XbaI T'CTAG_A Cuts at: 3836 3904 3836 Size: 68 6500 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3071 3071 Size: 6568 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6568 XmaI C'CCGG_G Cuts at: 1870 3893 1870 Size: 2023 4545 XmnI GAAnn'nnTTC Cuts at: 4697 4697 Size: 6568 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI