(Circular) (Six-base) MAPSORT of: pFN21AA0687.seq Check: 2449 from: 1 to: 7816 pFN21AA0687 7816 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5626 278 Size: 53 83 186 936 4090 2468 Fragments arranged by size: 4090 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2131 3053 4347 5159 1937 Size: 194 922 1294 812 4594 Fragments arranged by size: 4594 1294 922 812 194 Acc65I G'GTAC_C Cuts at: 5137 5137 Size: 7816 AclI AA'CG_TT Cuts at: 1563 3100 5943 6316 1563 Size: 1537 2843 373 3063 Fragments arranged by size: 3063 2843 1537 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2783 3460 3522 3567 5879 7263 7531 1632 Size: 1151 677 62 45 2312 1384 268 1917 Fragments arranged by size: 2312 1917 1384 1151 677 268 62 45 AflII C'TTAA_G Cuts at: 820 1017 2909 820 Size: 197 1892 5727 Fragments arranged by size: 5727 1892 197 AflIII A'CryG_T Cuts at: 1203 2134 4342 4708 6715 7692 1203 Size: 931 2208 366 2007 977 1327 Fragments arranged by size: 2208 2007 1327 977 931 366 AgeI A'CCGG_T Cuts at: 6647 6647 Size: 7816 AhdI GACnn_n'nnGTC Cuts at: 6545 6545 Size: 7816 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7816 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7816 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4964 4996 4964 Size: 32 7784 AlwNI CAG_nnn'CTG Cuts at: 3523 5559 7131 3523 Size: 2036 1572 4208 Fragments arranged by size: 4208 2036 1572 ApaLI G'TGCA_C Cuts at: 5873 7029 5873 Size: 1156 6660 ApoI r'AATT_y Cuts at: 1493 3781 4186 4478 4544 5009 5125 5351 5503 Size: 2288 405 292 66 465 116 226 152 Cuts at: 5503 7612 1493 Size: 2109 1697 Fragments arranged by size: 2288 2109 1697 465 405 292 226 152 116 66 AseI AT'TA_AT Cuts at: 160 1032 6370 160 Size: 872 5338 1606 Fragments arranged by size: 5338 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7816 AvaI C'yCGr_G Cuts at: 1870 1943 3185 3434 5141 1870 Size: 73 1242 249 1707 4545 Fragments arranged by size: 4545 1707 1242 249 73 BamHI G'GATC_C Cuts at: 2062 3425 4640 5146 5513 2062 Size: 1363 1215 506 367 4365 Fragments arranged by size: 4365 1363 1215 506 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2808 3379 4525 5137 Size: 325 217 627 318 703 571 1146 612 Cuts at: 5137 6592 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1146 703 627 612 571 325 318 217 BanII G_rGCy'C Cuts at: 1391 2285 3192 3348 4999 5135 1391 Size: 894 907 156 1651 136 4072 Fragments arranged by size: 4072 1651 907 894 156 136 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2391 4273 928 Size: 971 492 1882 4471 Fragments arranged by size: 4471 1882 971 492 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2003 2037 3862 3896 6007 6041 1903 Size: 34 66 34 1825 34 2111 34 3678 Fragments arranged by size: 3678 2111 1825 66 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5708 6918 5708 Size: 1210 6606 BclI T'GATC_A Cuts at: 1548 1548 Size: 7816 BfrBI ATG'CAT Cuts at: 2713 2713 Size: 7816 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6427 136 Size: 107 122 71 631 605 4755 1525 Fragments arranged by size: 4755 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7811 7811 Size: 7816 BlpI GC'TnA_GC Cuts at: 2532 5233 2532 Size: 2701 5115 Bme1580I G_kGCm'C Cuts at: 1790 3384 3875 4528 5877 7033 1790 Size: 1594 491 653 1349 1156 2573 Fragments arranged by size: 2573 1594 1349 1156 653 491 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7816 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5547 6505 442 Size: 416 53 83 399 24 4130 958 1753 Fragments arranged by size: 4130 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7816 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3760 3760 Size: 7816 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2271 2389 3899 6476 1239 Size: 989 43 118 1510 2577 2579 Fragments arranged by size: 2579 2577 1510 989 118 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3560 4313 5941 6806 7104 3560 Size: 753 1628 865 298 4272 Fragments arranged by size: 4272 1628 865 753 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3444 3456 4027 6479 882 Size: 617 1945 12 571 2452 2219 Fragments arranged by size: 2452 2219 1945 617 571 12 BsaAI yAC'GTr Cuts at: 493 1204 4343 5607 493 Size: 711 3139 1264 2702 Fragments arranged by size: 3139 2702 1264 711 BsaBI GATnn'nnATC Cuts at: 3490 7810 3490 Size: 4320 3496 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5623 6005 6640 275 Size: 53 83 186 936 4090 382 635 1451 Fragments arranged by size: 4090 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6249 6647 6921 7068 7479 6249 Size: 398 274 147 411 6586 Fragments arranged by size: 6586 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 4560 4590 1178 Size: 30 165 30 633 30 2494 30 4404 Fragments arranged by size: 4404 2494 633 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 2742 3008 3394 3658 3695 3716 3925 Size: 855 701 266 386 264 37 21 209 Cuts at: 3925 1186 Size: 5077 Fragments arranged by size: 5077 855 701 386 266 264 209 37 21 BseYI C'CCAG_C Cuts at: 2069 2333 3429 4923 5112 7019 7515 2069 Size: 264 1096 1494 189 1907 496 2370 Fragments arranged by size: 2370 1907 1494 1096 496 264 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7243 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5266 6027 7055 1540 Size: 275 186 3265 761 1028 2301 Fragments arranged by size: 3265 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2533 2695 2728 5877 5962 7033 2533 Size: 162 33 3149 85 1071 3316 Fragments arranged by size: 3316 3149 1071 162 85 33 BsmI GAATG_Cn' Cuts at: 2500 5327 5420 2500 Size: 2827 93 4896 Fragments arranged by size: 4896 2827 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 2533 2695 2728 3192 3348 3384 Size: 399 495 248 162 33 464 156 36 Cuts at: 3384 3875 4528 4999 5135 5877 5962 7033 1391 Size: 491 653 471 136 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 653 495 491 471 464 399 248 162 156 136 85 36 33 BspEI T'CCGG_A Cuts at: 7479 7479 Size: 7816 BspHI T'CATG_A Cuts at: 5082 5705 7435 5082 Size: 623 1730 5463 Fragments arranged by size: 5463 1730 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5171 844 Size: 4327 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5703 1394 Size: 429 3880 3507 Fragments arranged by size: 3880 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2247 3796 5032 6311 6485 65 Size: 1183 999 1549 1236 1279 174 1396 Fragments arranged by size: 1549 1396 1279 1236 1183 999 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3847 6460 6647 1700 Size: 110 2037 2613 187 2869 Fragments arranged by size: 2869 2613 2037 187 110 BsrGI T'GTAC_A Cuts at: 96 4444 96 Size: 4348 3468 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7816 BssSI C'ACGA_G Cuts at: 5876 6888 5876 Size: 1012 6804 BstAPI GCAn_nnn'nTGC Cuts at: 794 2690 794 Size: 1896 5920 BstBI TT'CG_AA Cuts at: 6620 6620 Size: 7816 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7816 BstXI CCAn_nnnn'nTGG Cuts at: 2680 2680 Size: 7816 BstYI r'GATC_y Cuts at: 1972 2062 3425 3595 4054 4597 4640 4944 5146 Size: 90 1363 170 459 543 43 304 202 Cuts at: 5146 5513 5898 5915 7367 7453 7465 7811 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1363 543 459 385 367 346 304 202 170 90 86 43 17 12 Bsu36I CC'TnA_GG Cuts at: 2417 2595 3406 3745 5089 2417 Size: 178 811 339 1344 5144 Fragments arranged by size: 5144 1344 811 339 178 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6325 Fragments arranged by size: 6325 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2587 487 Size: 683 56 1361 5716 Fragments arranged by size: 5716 1361 683 56 BtsI GCAGTG_nn' Cuts at: 3281 3298 3400 3540 3633 5332 6126 6146 3281 Size: 17 102 140 93 1699 794 20 4951 Fragments arranged by size: 4951 1699 794 140 102 93 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7816 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7781 DraI TTT'AAA Cuts at: 5120 5120 Size: 7816 DraIII CAC_nnn'GTG Cuts at: 4112 4112 Size: 7816 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6823 809 Size: 507 5507 1802 Fragments arranged by size: 5507 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3609 3641 3849 Size: 54 1425 50 275 18 1779 32 208 Cuts at: 3849 4234 5263 6152 7781 8 Size: 385 1029 889 1629 43 Fragments arranged by size: 1779 1629 1425 1029 889 385 275 208 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 5263 1537 Size: 275 3451 4090 Fragments arranged by size: 4090 3451 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2933 2985 3063 3117 4136 4493 5109 Size: 831 749 52 78 54 1019 357 616 Cuts at: 5109 5746 1353 Size: 637 3423 Fragments arranged by size: 3423 1019 831 749 637 616 357 78 54 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2569 3875 6403 6777 6923 387 Size: 1064 138 980 1306 2528 374 146 1280 Fragments arranged by size: 2528 1306 1280 1064 980 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2271 2389 2783 3460 3522 3567 Size: 393 596 43 118 394 677 62 45 Cuts at: 3567 3899 5879 6476 7263 7531 1239 Size: 332 1980 597 787 268 1524 Fragments arranged by size: 1980 1524 787 677 597 596 394 393 332 268 118 62 45 43 EcoNI CCTnn'n_nnAGG Cuts at: 5092 5092 Size: 7816 EcoRI G'AATT_C Cuts at: 5125 5503 5125 Size: 378 7438 EcoRV GAT'ATC Cuts at: 2464 2464 Size: 7816 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3190 5056 3190 Size: 1866 5950 FspI TGC'GCA Cuts at: 6322 6322 Size: 7816 HaeII r_GCGC'y Cuts at: 1678 3523 6963 1678 Size: 1845 3440 2531 Fragments arranged by size: 3440 2531 1845 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2639 2671 3653 Size: 32 135 32 464 32 571 32 982 Cuts at: 3653 3685 4664 4696 4934 4966 6457 6489 6531 Size: 32 979 32 238 32 1491 32 42 Cuts at: 6531 6563 1373 Size: 32 2626 Fragments arranged by size: 2626 1491 982 979 571 464 238 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2132 2515 5160 5406 669 Size: 988 281 194 383 2645 246 3079 Fragments arranged by size: 3079 2645 988 383 281 246 194 HindIII A'AGCT_T Cuts at: 748 3172 748 Size: 2424 5392 HpaI GTT'AAC Cuts at: 5406 5406 Size: 7816 KpnI G_GTAC'C Cuts at: 5141 5141 Size: 7816 MfeI C'AATT_G Cuts at: 5415 5415 Size: 7816 MluI A'CGCG_T Cuts at: 7692 7692 Size: 7816 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2077 6930 7114 1305 Size: 772 4853 184 2007 Fragments arranged by size: 4853 2007 772 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3611 4236 10 Size: 54 1425 343 1779 625 3590 Fragments arranged by size: 3590 1779 1425 625 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2678 3831 4971 5774 6133 6292 518 Size: 648 1512 1153 1140 803 359 159 2042 Fragments arranged by size: 2042 1512 1153 1140 803 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2536 2833 5232 5909 7057 7302 1606 Size: 930 297 2399 677 1148 245 2120 Fragments arranged by size: 2399 2120 1148 930 677 297 245 NaeI GCC'GGC Cuts at: 3849 3849 Size: 7816 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6325 Fragments arranged by size: 6325 939 552 NdeI CA'TA_TG Cuts at: 387 4837 387 Size: 4450 3366 NgoMIV G'CCGG_C Cuts at: 3847 3847 Size: 7816 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7816 NotI GC'GGCC_GC Cuts at: 5263 5263 Size: 7816 NsiI A_TGCA'T Cuts at: 2715 2715 Size: 7816 NspI r_CATG'y Cuts at: 1132 1229 2138 2713 2830 4712 5174 6719 1132 Size: 97 909 575 117 1882 462 1545 2229 Fragments arranged by size: 2229 1882 1545 909 575 462 117 97 PasI CC'CwG_GG Cuts at: 1348 3446 1348 Size: 2098 5718 PciI A'CATG_T Cuts at: 2134 4708 6715 2134 Size: 2574 2007 3235 Fragments arranged by size: 3235 2574 2007 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7816 PfoI T'CCnGG_A Cuts at: 2287 3147 2287 Size: 860 6956 PmeI GTTT'AAAC Cuts at: 5120 5120 Size: 7816 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3232 3264 4712 4744 5885 5917 7398 7430 3232 Size: 32 1448 32 1141 32 1481 32 3618 Fragments arranged by size: 3618 1481 1448 1141 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7816 PsiI TTA'TAA Cuts at: 2870 4452 5386 2870 Size: 1582 934 5300 Fragments arranged by size: 5300 1582 934 PstI C_TGCA'G Cuts at: 830 3084 3288 5168 830 Size: 2254 204 1880 3478 Fragments arranged by size: 3478 2254 1880 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7816 PvuII CAG'CTG Cuts at: 2536 2536 Size: 7816 SalI G'TCGA_C Cuts at: 1936 2130 5158 1936 Size: 194 3028 4594 Fragments arranged by size: 4594 3028 194 SapI GCTCTTCn'nnn_ Cuts at: 3117 3117 Size: 7816 SbfI CC_TGCA'GG Cuts at: 5168 5168 Size: 7816 ScaI AGT'ACT Cuts at: 6064 7776 6064 Size: 1712 6104 SfcI C'TryA_G Cuts at: 826 1045 2841 3080 3131 3284 3968 4865 5164 Size: 219 1796 239 51 153 684 897 299 Cuts at: 5164 6299 6980 7171 826 Size: 1135 681 191 1471 Fragments arranged by size: 1796 1471 1135 897 684 681 299 239 219 191 153 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7816 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7816 SmaI CCC'GGG Cuts at: 1872 3436 5143 1872 Size: 1564 1707 4545 Fragments arranged by size: 4545 1707 1564 SmlI C'TyrA_G Cuts at: 820 1017 1943 2909 3575 4292 5920 6821 7083 Size: 197 926 966 666 717 1628 901 262 Cuts at: 7083 820 Size: 1553 Fragments arranged by size: 1628 1553 966 926 901 717 666 262 197 SnaBI TAC'GTA Cuts at: 493 5607 493 Size: 5114 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7816 SphI G_CATG'C Cuts at: 1132 5174 1132 Size: 4042 3774 SspI AAT'ATT Cuts at: 5 52 5740 5 Size: 47 5688 2081 Fragments arranged by size: 5688 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7816 StyI C'CwwG_G Cuts at: 513 1065 2004 3864 4237 4851 5255 513 Size: 552 939 1860 373 614 404 3074 Fragments arranged by size: 3074 1860 939 614 552 404 373 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3231 5043 5843 5860 6013 6198 6643 Size: 1255 1651 1812 800 17 153 185 445 Cuts at: 6643 325 Size: 1498 Fragments arranged by size: 1812 1651 1498 1255 800 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2970 4444 6062 Size: 274 80 33 51 1443 993 1474 1618 Cuts at: 6062 7774 96 Size: 1712 138 Fragments arranged by size: 1712 1618 1474 1443 993 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3914 3946 3981 4013 7776 7808 3914 Size: 32 35 32 3763 32 3922 Fragments arranged by size: 3922 3763 35 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3895 1316 Size: 2579 5237 XbaI T'CTAG_A Cuts at: 5152 5152 Size: 7816 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 4915 2167 Size: 2748 5068 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7816 XmaI C'CCGG_G Cuts at: 1870 3434 5141 1870 Size: 1564 1707 4545 Fragments arranged by size: 4545 1707 1564 XmnI GAAnn'nnTTC Cuts at: 5945 5945 Size: 7816 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5624 276 Size: 53 83 186 936 4090 2468 Fragments arranged by size: 4090 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoO109I FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI