Restriction map for
pFN21AA0687
(Linear) (Six-base) MAPSORT of: KIAA0687.seq Check: 6301 from: 1 to: 3111
KIAA0687.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 126 1048 2342 3111
Size: 126 922 1294 769
Fragments arranged by size:
1294 922 769 126
AclI AA'CG_TT
Cuts at: 0 1095 3111
Size: 1095 2016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1455 1517 1562 3111
Size: 778 677 62 45 1549
Fragments arranged by size:
1549 778 677 62 45
AflII C'TTAA_G
Cuts at: 0 904 3111
Size: 904 2207
AflIII A'CryG_T
Cuts at: 0 129 2337 2703 3111
Size: 129 2208 366 408
Fragments arranged by size:
2208 408 366 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2959 2991 3111
Size: 2959 32 120
Fragments arranged by size:
2959 120 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1518 3111
Size: 1518 1593
ApoI r'AATT_y
Cuts at: 0 1776 2181 2473 2539 3004 3111
Size: 1776 405 292 66 465 107
Fragments arranged by size:
1776 465 405 292 107 66
AvaI C'yCGr_G
Cuts at: 0 1180 1429 3111
Size: 1180 249 1682
Fragments arranged by size:
1682 1180 249
BamHI G'GATC_C
Cuts at: 0 57 1420 2635 3111
Size: 57 1363 1215 476
Fragments arranged by size:
1363 1215 476 57
BanI G'GyrC_C
Cuts at: 0 100 803 1374 2520 3111
Size: 100 703 571 1146 591
Fragments arranged by size:
1146 703 591 571 100
BanII G_rGCy'C
Cuts at: 0 280 1187 1343 2994 3111
Size: 280 907 156 1651 117
Fragments arranged by size:
1651 907 280 156 117
BbsI GAAGACnn'nnnn_
Cuts at: 0 386 2268 3111
Size: 386 1882 843
Fragments arranged by size:
1882 843 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 1857 1891 3111
Size: 32 1825 34 1220
Fragments arranged by size:
1825 1220 34 32
BfrBI ATG'CAT
Cuts at: 0 708 3111
Size: 708 2403
BlpI GC'TnA_GC
Cuts at: 0 527 3111
Size: 527 2584
Bme1580I G_kGCm'C
Cuts at: 0 1379 1870 2523 3111
Size: 1379 491 653 588
Fragments arranged by size:
1379 653 588 491
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1755 3111
Size: 1755 1356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 266 384 1894 3111
Size: 223 43 118 1510 1217
Fragments arranged by size:
1510 1217 223 118 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1555 2308 3111
Size: 1555 753 803
Fragments arranged by size:
1555 803 753
BsaI GGTCTCn'nnnn_
Cuts at: 0 1439 1451 2022 3111
Size: 1439 12 571 1089
Fragments arranged by size:
1439 1089 571 12
BsaAI yAC'GTr
Cuts at: 0 2338 3111
Size: 2338 773
BsaBI GATnn'nnATC
Cuts at: 0 1485 3111
Size: 1485 1626
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 2555 2585 3111
Size: 31 30 2494 30 526
Fragments arranged by size:
2494 526 31 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 36 737 1003 1389 1653 1690 1711 1920
Size: 36 701 266 386 264 37 21 209
Cuts at: 1920 3111
Size: 1191
Fragments arranged by size:
1191 701 386 266 264 209 37 36
21
BseYI C'CCAG_C
Cuts at: 0 64 328 1424 2918 3111
Size: 64 264 1096 1494 193
Fragments arranged by size:
1494 1096 264 193 64
BsiHKAI G_wGCw'C
Cuts at: 0 528 690 723 3111
Size: 528 162 33 2388
Fragments arranged by size:
2388 528 162 33
BsmI GAATG_Cn'
Cuts at: 0 495 3111
Size: 495 2616
Bsp1286I G_dGCh'C
Cuts at: 0 280 528 690 723 1187 1343 1379 1870
Size: 280 248 162 33 464 156 36 491
Cuts at: 1870 2523 2994 3111
Size: 653 471 117
Fragments arranged by size:
653 491 471 464 280 248 162 156
117 36 33
BspHI T'CATG_A
Cuts at: 0 3077 3111
Size: 3077 34
BsrDI GCAATG_nn'
Cuts at: 0 242 1791 3027 3111
Size: 242 1549 1236 84
Fragments arranged by size:
1549 1236 242 84
BsrFI r'CCGG_y
Cuts at: 0 1842 3111
Size: 1842 1269
BsrGI T'GTAC_A
Cuts at: 0 2439 3111
Size: 2439 672
BstAPI GCAn_nnn'nTGC
Cuts at: 0 685 3111
Size: 685 2426
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 3111
Size: 675 2436
BstYI r'GATC_y
Cuts at: 0 57 1420 1590 2049 2592 2635 2939 3111
Size: 57 1363 170 459 543 43 304 172
Fragments arranged by size:
1363 543 459 304 172 170 57 43
Bsu36I CC'TnA_GG
Cuts at: 0 412 590 1401 1740 3084 3111
Size: 412 178 811 339 1344 27
Fragments arranged by size:
1344 811 412 339 178 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 582 3111
Size: 582 2529
BtsI GCAGTG_nn'
Cuts at: 0 1276 1293 1395 1535 1628 3111
Size: 1276 17 102 140 93 1483
Fragments arranged by size:
1483 1276 140 102 93 17
DraIII CAC_nnn'GTG
Cuts at: 0 2107 3111
Size: 2107 1004
EaeI y'GGCC_r
Cuts at: 0 1604 1636 1844 2229 3111
Size: 1604 32 208 385 882
Fragments arranged by size:
1604 882 385 208 32
EarI CTCTTCn'nnn_
Cuts at: 0 179 928 980 1058 1112 2131 2488 3104
Size: 179 749 52 78 54 1019 357 616
Cuts at: 3104 3111
Size: 7
Fragments arranged by size:
1019 749 616 357 179 78 54 52
7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 564 1870 3111
Size: 564 1306 1241
Fragments arranged by size:
1306 1241 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 266 384 778 1455 1517 1562 1894
Size: 223 43 118 394 677 62 45 332
Cuts at: 1894 3111
Size: 1217
Fragments arranged by size:
1217 677 394 332 223 118 62 45
43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3087 3111
Size: 3087 24
EcoRV GAT'ATC
Cuts at: 0 459 3111
Size: 459 2652
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1185 3051 3111
Size: 1185 1866 60
Fragments arranged by size:
1866 1185 60
HaeII r_GCGC'y
Cuts at: 0 1518 3111
Size: 1518 1593
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 634 666 1648 1680 2659 2691
Size: 31 32 571 32 982 32 979 32
Cuts at: 2691 2929 2961 3111
Size: 238 32 150
Fragments arranged by size:
982 979 571 238 150 32 32 32
32 32 31
HincII GTy'rAC
Cuts at: 0 127 510 3111
Size: 127 383 2601
Fragments arranged by size:
2601 383 127
HindIII A'AGCT_T
Cuts at: 0 1167 3111
Size: 1167 1944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 3111
Size: 72 3039
MscI TGG'CCA
Cuts at: 0 1606 2231 3111
Size: 1606 625 880
Fragments arranged by size:
1606 880 625
MslI CAynn'nnrTG
Cuts at: 0 673 1826 2966 3111
Size: 673 1153 1140 145
Fragments arranged by size:
1153 1140 673 145
MspA1I CmG'CkG
Cuts at: 0 531 828 3111
Size: 531 297 2283
Fragments arranged by size:
2283 531 297
NaeI GCC'GGC
Cuts at: 0 1844 3111
Size: 1844 1267
NdeI CA'TA_TG
Cuts at: 0 2832 3111
Size: 2832 279
NgoMIV G'CCGG_C
Cuts at: 0 1842 3111
Size: 1842 1269
NsiI A_TGCA'T
Cuts at: 0 710 3111
Size: 710 2401
NspI r_CATG'y
Cuts at: 0 133 708 825 2707 3111
Size: 133 575 117 1882 404
Fragments arranged by size:
1882 575 404 133 117
PasI CC'CwG_GG
Cuts at: 0 1441 3111
Size: 1441 1670
PciI A'CATG_T
Cuts at: 0 129 2703 3111
Size: 129 2574 408
Fragments arranged by size:
2574 408 129
PfoI T'CCnGG_A
Cuts at: 0 282 1142 3111
Size: 282 860 1969
Fragments arranged by size:
1969 860 282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1227 1259 2707 2739 3111
Size: 1227 32 1448 32 372
Fragments arranged by size:
1448 1227 372 32 32
PsiI TTA'TAA
Cuts at: 0 865 2447 3111
Size: 865 1582 664
Fragments arranged by size:
1582 865 664
PstI C_TGCA'G
Cuts at: 0 1079 1283 3111
Size: 1079 204 1828
Fragments arranged by size:
1828 1079 204
PvuII CAG'CTG
Cuts at: 0 531 3111
Size: 531 2580
SalI G'TCGA_C
Cuts at: 0 125 3111
Size: 125 2986
SapI GCTCTTCn'nnn_
Cuts at: 0 1112 3111
Size: 1112 1999
SfcI C'TryA_G
Cuts at: 0 836 1075 1126 1279 1963 2860 3111
Size: 836 239 51 153 684 897 251
Fragments arranged by size:
897 836 684 251 239 153 51
SmaI CCC'GGG
Cuts at: 0 1431 3111
Size: 1431 1680
SmlI C'TyrA_G
Cuts at: 0 904 1570 2287 3111
Size: 904 666 717 824
Fragments arranged by size:
904 824 717 666
StyI C'CwwG_G
Cuts at: 0 1859 2232 2846 3111
Size: 1859 373 614 265
Fragments arranged by size:
1859 614 373 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1226 3038 3111
Size: 1226 1812 73
Fragments arranged by size:
1812 1226 73
TatI w'GTAC_w
Cuts at: 0 965 2439 3111
Size: 965 1474 672
Fragments arranged by size:
1474 965 672
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1909 1941 1976 2008 3111
Size: 1909 32 35 32 1103
Fragments arranged by size:
1909 1103 35 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1890 3111
Size: 1890 1221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 2910 3111
Size: 162 2748 201
Fragments arranged by size:
2748 201 162
XmaI C'CCGG_G
Cuts at: 0 1429 3111
Size: 1429 1682
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AloI AlwNI ApoI
AvaI BamHI BanI BanII BbsI BcgI BfrBI BlpI
Bme1580I BplI BpmI BpuEI BsaI BsaAI BsaBI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI
BsrGI BstAPI BstXI BstYI Bsu36I BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoRV FalI HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI
NdeI NgoMIV NsiI NspI PasI PciI PfoI PpiI
PsiI PstI PvuII SalI SapI SfcI SmaI SmlI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BciVI BclI BglI BglII BmgBI BmrI BmtI
Bpu10I BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BssHII BssSI BstBI BstEII BstZ17I BtgI
ClaI CspCI DraI DrdI EagI EcoICRI EcoO109I EcoRI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NarI NcoI NheI NotI NruI PacI PflMI PmeI
PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmnI ZraI