Restriction map for
pFN21AA0589
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# NO CUT ENZYMES ( 94 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI AocI ApoI AseI
AsnI AviII AvrII BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrGI Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI MamI
MfeI MluI MroI MseI MunI
NdeI NotI NruI NsiI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1626 GACGTC GACGT^C
Acc65I 12 GGTACC G^GTACC
AhdI 1261 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 967 GTGCAC G^TGCAC
AlwNI 595 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 967 GTGCAC G^TGCAC
AscI 782 GGCGCGCC GG^CGCGCC
Asp700I 807 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 12 GGTACC G^GTACC
AspEI 1261 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1578 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 156 TGGCCA TGG^CCA
BbsI 216 GAAGAC GAAGAC(2/6)
BbvCI 34 GCTGAGG CCTCAGC(-5/-2)
BpuAI 216 GAAGAC GAAGAC(2/6)
BsaAI 322 TACGTG [CT]AC^GT[GA]
BsaWI 15 ACCGGA [AT]^CCGG[AT]
BsmAI 1624 GAGAC GTCTC(1/5)
BsmBI 1624 GAGACG CGTCTC(1/5)
BssSI 985 CACGAG CACGAG(-5/-1)
BstBI 1546 TTCGAA TT^CGAA
DrdI 913 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1261 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 1510 GGCCGGCC GGCCGG^CC
KpnI 12 GGTACC GGTAC^C
MaeIII 535 GTCAC ^GT[ACGT]AC
MluNI 156 TGGCCA TGG^CCA
MscI 156 TGGCCA TGG^CCA
NaeI 1511 GCCGGC GCC^GGC
NgoMIV 1511 GCCGGC G^CCGGC
NheI 1389 GCTAGC G^CTAGC
NspI 848 ACATGC [GA]CATG^[CT]
NspV 1546 TTCGAA TT^CGAA
PflFI 1578 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 827 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 250 CAGCTG CAG^CTG
SapI 807 GAAGAGC GCTCTTC(1/4)
SfiI 1971 GGCCCCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1546 TTCGAA TT^CGAA
SmaI 507 CCCGGG CCC^GGG
TfiI 1114 GAATC G^A[AT]TC
Tsp45I 535 GTCAC ^GT[GC]AC
Tth111I 1578 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 507 CCCGGG C^CCGGG
XmaI 507 CCCGGG C^CCGGG
XmnI 807 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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Aor51HI 31 AGCGCT AGC^GCT
1298 AGCGCT
AvaI 507 CCCGGG C^[CT]CG[GA]G
853 CCCGAG
BamHI 446 GGATCC G^GATCC
1136 GGATCC
BfaI 1390 CTAG C^TAG
1442 CTAG
BsiEI 1152 CGGCCG CG[GA][CT]^CG
1802 CGGCCG
BsoBI 507 CCCGGG C^[CT]CG[GA]G
853 CCCGAG
BspHI 710 TCATGA T^CATGA
1313 TCATGA
BsrDI 1569 CATTGC GCAATG(2/0)
479 GCAATG
BsrFI 1511 GCCGGC [GA]^CCGG[CT]
1804 GCCGGT
BssHII 783 GCGCGC G^CGCGC
1096 GCGCGC
BstAPI 594 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
843 GCAGGACATGC
BstXI 245 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1064 CCACGGCCATGG
Cfr10I 1511 GCCGGC [GA]^CCGG[CT]
1804 GCCGGT
EagI 1152 CGGCCG C^GGCCG
1802 CGGCCG
EclXI 1152 CGGCCG C^GGCCG
1802 CGGCCG
Eco47III 31 AGCGCT AGC^GCT
1298 AGCGCT
Eco52I 1152 CGGCCG C^GGCCG
1802 CGGCCG
EcoT14I 159 CCATGG C^C[AT][AT]GG
1070 CCATGG
HphI 365 TCACC GGTGA(8/7)
536 TCACC
MaeI 1390 CTAG C^TAG
1442 CTAG
MslI 178 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1065 CACGGCCATG
NcoI 159 CCATGG C^CATGG
1070 CCATGG
PleI 1578 GACTC GAGTC(4/5)
1075 GAGTC
RcaI 710 TCATGA T^CATGA
1313 TCATGA
SacI 282 GAGCTC GAGCT^C
436 GAGCTC
SmlI 748 CTCAAG C^T[CT][GA]AG
760 CTCAAG
StuI 1844 AGGCCT AGG^CCT
1877 AGGCCT
StyI 159 CCATGG C^C[AT][AT]GG
1070 CCATGG
TspRI 516 CAGTG CA[GC]TG[ACGT][ACGT]^
1727 CAGTG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI Ksp632I
KspI MaeII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PstI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
TaqI TseI TthHB8I XhoII