Restriction map for
pFN21AA0589
(Linear) (Six-base) MAPSORT of: KIAA0589.seq Check: 4395 from: 1 to: 2004
KIAA0589.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1722 1875 2004
Size: 1722 153 129
Fragments arranged by size:
1722 153 129
AatII G_ACGT'C
Cuts at: 0 1630 2004
Size: 1630 374
Acc65I G'GTAC_C
Cuts at: 0 12 2004
Size: 12 1992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 867 1369 2004
Size: 256 611 502 635
Fragments arranged by size:
635 611 502 256
AfeI AGC'GCT
Cuts at: 0 33 1300 2004
Size: 33 1267 704
Fragments arranged by size:
1267 704 33
AhdI GACnn_n'nnGTC
Cuts at: 0 1266 2004
Size: 1266 738
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 571 603 2004
Size: 571 32 1401
Fragments arranged by size:
1401 571 32
AlwNI CAG_nnn'CTG
Cuts at: 0 600 2004
Size: 600 1404
ApaI G_GGCC'C
Cuts at: 0 154 1412 1482 2004
Size: 154 1258 70 522
Fragments arranged by size:
1258 522 154 70
ApaLI G'TGCA_C
Cuts at: 0 967 2004
Size: 967 1037
AscI GG'CGCG_CC
Cuts at: 0 783 2004
Size: 783 1221
AvaI C'yCGr_G
Cuts at: 0 507 853 2004
Size: 507 346 1151
Fragments arranged by size:
1151 507 346
BamHI G'GATC_C
Cuts at: 0 446 1136 2004
Size: 446 690 868
Fragments arranged by size:
868 690 446
BanI G'GyrC_C
Cuts at: 0 12 241 511 1009 1090 1807 2004
Size: 12 229 270 498 81 717 197
Fragments arranged by size:
717 498 270 229 197 81 12
BanII G_rGCy'C
Cuts at: 0 154 286 440 769 863 1412 1482 2004
Size: 154 132 154 329 94 549 70 522
Fragments arranged by size:
549 522 329 154 154 132 94 70
BbeI G_GCGC'C
Cuts at: 0 515 1013 1094 2004
Size: 515 498 81 910
Fragments arranged by size:
910 515 498 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 223 2004
Size: 223 1781
BbvCI CC'TCA_GC
Cuts at: 0 35 2004
Size: 35 1969
BglI GCCn_nnn'nGGC
Cuts at: 0 858 1426 1843 1876 1978 2004
Size: 858 568 417 33 102 26
Fragments arranged by size:
858 568 417 102 33 26
Bme1580I G_kGCm'C
Cuts at: 0 53 154 971 1412 1482 2004
Size: 53 101 817 441 70 522
Fragments arranged by size:
817 522 441 101 70 53
BmgBI CAC'GTC
Cuts at: 0 303 2004
Size: 303 1701
BmrI ACTGGGnnnn_n'
Cuts at: 0 1455 2004
Size: 1455 549
BmtI G_CTAG'C
Cuts at: 0 1393 2004
Size: 1393 611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1246 1519 2004
Size: 28 1218 273 485
Fragments arranged by size:
1218 485 273 28
Bpu10I CC'TnA_GC
Cuts at: 0 35 280 1607 2004
Size: 35 245 1327 397
Fragments arranged by size:
1327 397 245 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 745 2004
Size: 733 12 1259
Fragments arranged by size:
1259 733 12
BsaAI yAC'GTr
Cuts at: 0 324 2004
Size: 324 1680
BsaHI Gr'CG_yC
Cuts at: 0 512 872 1010 1091 1627 2004
Size: 512 360 138 81 536 377
Fragments arranged by size:
536 512 377 360 138 81
BsaWI w'CCGG_w
Cuts at: 0 15 2004
Size: 15 1989
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 268 298 454 484 571 601
Size: 49 30 189 30 156 30 87 30
Cuts at: 601 1447 1477 1729 1759 2004
Size: 846 30 252 30 245
Fragments arranged by size:
846 252 245 189 156 87 49 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 1332 1468 1705 1789 1870 1903 1947
Size: 216 1116 136 237 84 81 33 44
Cuts at: 1947 2004
Size: 57
Fragments arranged by size:
1116 237 216 136 84 81 57 44
33
BseYI C'CCAG_C
Cuts at: 0 252 346 960 1282 1404 1937 2004
Size: 252 94 614 322 122 533 67
Fragments arranged by size:
614 533 322 252 122 94 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1159 1515 1750 1767 2004
Size: 697 462 356 235 17 237
Fragments arranged by size:
697 462 356 237 235 17
BsiEI CG_ry'CG
Cuts at: 0 1155 1805 2004
Size: 1155 650 199
Fragments arranged by size:
1155 650 199
BsiHKAI G_wGCw'C
Cuts at: 0 286 308 440 971 1021 1232 2004
Size: 286 22 132 531 50 211 772
Fragments arranged by size:
772 531 286 211 132 50 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1618 2004
Size: 1618 386
Bsp1286I G_dGCh'C
Cuts at: 0 53 154 286 308 440 769 863 971
Size: 53 101 132 22 132 329 94 108
Cuts at: 971 1021 1232 1412 1482 2004
Size: 50 211 180 70 522
Fragments arranged by size:
522 329 211 180 132 132 108 101
94 70 53 50 22
BspHI T'CATG_A
Cuts at: 0 710 1313 2004
Size: 710 603 691
Fragments arranged by size:
710 691 603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 82 1478 1722 1875 2004
Size: 82 1396 244 153 129
Fragments arranged by size:
1396 244 153 129 82
BsrBI CCG'CTC
Cuts at: 0 80 996 1164 1449 1611 1953 2004
Size: 80 916 168 285 162 342 51
Fragments arranged by size:
916 342 285 168 162 80 51
BsrDI GCAATG_nn'
Cuts at: 0 486 1568 2004
Size: 486 1082 436
Fragments arranged by size:
1082 486 436
BsrFI r'CCGG_y
Cuts at: 0 1511 1804 2004
Size: 1511 293 200
Fragments arranged by size:
1511 293 200
BssHII G'CGCG_C
Cuts at: 0 783 1096 2004
Size: 783 313 908
Fragments arranged by size:
908 783 313
BssSI C'ACGA_G
Cuts at: 0 985 2004
Size: 985 1019
BstAPI GCAn_nnn'nTGC
Cuts at: 0 600 849 2004
Size: 600 249 1155
Fragments arranged by size:
1155 600 249
BstBI TT'CG_AA
Cuts at: 0 1547 2004
Size: 1547 457
BstXI CCAn_nnnn'nTGG
Cuts at: 0 252 1071 2004
Size: 252 819 933
Fragments arranged by size:
933 819 252
BstYI r'GATC_y
Cuts at: 0 446 1136 1436 1710 2004
Size: 446 690 300 274 294
Fragments arranged by size:
690 446 300 294 274
BtgI C'CryG_G
Cuts at: 0 159 269 637 1064 1070 1155 1639 1700
Size: 159 110 368 427 6 85 484 61
Cuts at: 1700 2004
Size: 304
Fragments arranged by size:
484 427 368 304 159 110 85 61
6
DrdI GACnn_nn'nnGTC
Cuts at: 0 919 2004
Size: 919 1085
EaeI y'GGCC_r
Cuts at: 0 156 451 1067 1152 1427 1802 2004
Size: 156 295 616 85 275 375 202
Fragments arranged by size:
616 375 295 275 202 156 85
EagI C'GGCC_G
Cuts at: 0 1152 1802 2004
Size: 1152 650 202
Fragments arranged by size:
1152 650 202
EarI CTCTTCn'nnn_
Cuts at: 0 532 802 1700 2004
Size: 532 270 898 304
Fragments arranged by size:
898 532 304 270
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 1394 2004
Size: 40 1354 610
Fragments arranged by size:
1354 610 40
EcoICRI GAG'CTC
Cuts at: 0 284 438 2004
Size: 284 154 1566
Fragments arranged by size:
1566 284 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 256 867 1246 1369 1519 2004
Size: 28 228 611 379 123 150 485
Fragments arranged by size:
611 485 379 228 150 123 28
EcoO109I rG'GnC_Cy
Cuts at: 0 151 828 1242 1408 1478 1971 2004
Size: 151 677 414 166 70 493 33
Fragments arranged by size:
677 493 414 166 151 70 33
FseI GG_CCGG'CC
Cuts at: 0 1515 2004
Size: 1515 489
HaeII r_GCGC'y
Cuts at: 0 35 515 890 1013 1057 1094 1302 1967
Size: 35 480 375 123 44 37 208 665
Cuts at: 1967 2004
Size: 37
Fragments arranged by size:
665 480 375 208 123 44 37 37
35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 1252 1284 2004
Size: 115 32 1105 32 720
Fragments arranged by size:
1105 720 115 32 32
KasI G'GCGC_C
Cuts at: 0 511 1009 1090 2004
Size: 511 498 81 914
Fragments arranged by size:
914 511 498 81
KpnI G_GTAC'C
Cuts at: 0 16 2004
Size: 16 1988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 1610 2004
Size: 527 1083 394
Fragments arranged by size:
1083 527 394
MscI TGG'CCA
Cuts at: 0 158 2004
Size: 158 1846
MslI CAynn'nnrTG
Cuts at: 0 182 1069 2004
Size: 182 887 935
Fragments arranged by size:
935 887 182
MspA1I CmG'CkG
Cuts at: 0 252 489 639 909 1144 1157 1404 1417
Size: 252 237 150 270 235 13 247 13
Cuts at: 1417 1641 1702 2004
Size: 224 61 302
Fragments arranged by size:
302 270 252 247 237 235 224 150
61 13 13
NaeI GCC'GGC
Cuts at: 0 1513 2004
Size: 1513 491
NarI GG'CG_CC
Cuts at: 0 512 1010 1091 2004
Size: 512 498 81 913
Fragments arranged by size:
913 512 498 81
NcoI C'CATG_G
Cuts at: 0 159 1070 2004
Size: 159 911 934
Fragments arranged by size:
934 911 159
NgoMIV G'CCGG_C
Cuts at: 0 1511 2004
Size: 1511 493
NheI G'CTAG_C
Cuts at: 0 1389 2004
Size: 1389 615
NspI r_CATG'y
Cuts at: 0 852 2004
Size: 852 1152
PfoI T'CCnGG_A
Cuts at: 0 383 431 923 2004
Size: 383 48 492 1081
Fragments arranged by size:
1081 492 383 48
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 571 603 2004
Size: 571 32 1401
Fragments arranged by size:
1401 571 32
PpuMI rG'GwC_Cy
Cuts at: 0 828 2004
Size: 828 1176
PspOMI G'GGCC_C
Cuts at: 0 150 1408 1478 2004
Size: 150 1258 70 526
Fragments arranged by size:
1258 526 150 70
PstI C_TGCA'G
Cuts at: 0 596 908 1202 1718 2004
Size: 596 312 294 516 286
Fragments arranged by size:
596 516 312 294 286
PvuII CAG'CTG
Cuts at: 0 252 2004
Size: 252 1752
SacI G_AGCT'C
Cuts at: 0 286 440 2004
Size: 286 154 1564
Fragments arranged by size:
1564 286 154
SacII CC_GC'GG
Cuts at: 0 640 1158 1642 1703 2004
Size: 640 518 484 61 301
Fragments arranged by size:
640 518 484 301 61
SapI GCTCTTCn'nnn_
Cuts at: 0 802 2004
Size: 802 1202
SfcI C'TryA_G
Cuts at: 0 592 904 948 1198 1206 1559 1714 1959
Size: 592 312 44 250 8 353 155 245
Cuts at: 1959 2004
Size: 45
Fragments arranged by size:
592 353 312 250 245 155 45 44
8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1978 2004
Size: 1978 26
SfoI GGC'GCC
Cuts at: 0 513 1011 1092 2004
Size: 513 498 81 912
Fragments arranged by size:
912 513 498 81
SmaI CCC'GGG
Cuts at: 0 509 2004
Size: 509 1495
SmlI C'TyrA_G
Cuts at: 0 748 760 2004
Size: 748 12 1244
Fragments arranged by size:
1244 748 12
StuI AGG'CCT
Cuts at: 0 1846 1879 2004
Size: 1846 33 125
Fragments arranged by size:
1846 125 33
StyI C'CwwG_G
Cuts at: 0 159 1070 2004
Size: 159 911 934
Fragments arranged by size:
934 911 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1929 2004
Size: 1929 75
TatI w'GTAC_w
Cuts at: 0 467 668 1943 2004
Size: 467 201 1275 61
Fragments arranged by size:
1275 467 201 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 268 300 1729 1761 2004
Size: 49 32 187 32 1429 32 243
Fragments arranged by size:
1429 243 187 49 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1581 2004
Size: 1581 423
XmaI C'CCGG_G
Cuts at: 0 507 2004
Size: 507 1497
XmnI GAAnn'nnTTC
Cuts at: 0 811 2004
Size: 811 1193
ZraI GAC'GTC
Cuts at: 0 1628 2004
Size: 1628 376
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AhdI AloI AlwNI
ApaI ApaLI AscI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BglI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FseI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII
SapI SfcI SfiI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AleI AlfI ApoI
AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI
BglII BlpI BplI BsaI BsaBI BsiWI BsmI BspEI
BsrGI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EcoNI EcoRI EcoRV FalI FspI FspAI
HincII HindIII HpaI MfeI MluI NdeI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XcmI XhoI