(Circular) (Six-base) MAPSORT of: pFN21AA0567.seq Check: 1760 from: 1 to: 7585 pFN21AA0567 7585 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5395 278 Size: 53 83 186 936 3859 2468 Fragments arranged by size: 3859 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3411 4651 4928 1937 Size: 1474 1240 277 4594 Fragments arranged by size: 4594 1474 1240 277 Acc65I G'GTAC_C Cuts at: 4906 4906 Size: 7585 AclI AA'CG_TT Cuts at: 1563 5712 6085 1563 Size: 4149 373 3063 Fragments arranged by size: 4149 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 2832 4728 4779 5648 7032 7300 1632 Size: 1010 190 1896 51 869 1384 268 1917 Fragments arranged by size: 1917 1896 1384 1010 869 268 190 51 AflII C'TTAA_G Cuts at: 820 1017 2783 820 Size: 197 1766 5622 Fragments arranged by size: 5622 1766 197 AflIII A'CryG_T Cuts at: 1203 2802 2972 3713 4798 6484 7461 1203 Size: 1599 170 741 1085 1686 977 1327 Fragments arranged by size: 1686 1599 1327 1085 977 741 170 AgeI A'CCGG_T Cuts at: 6416 6416 Size: 7585 AhdI GACnn_n'nnGTC Cuts at: 6314 6314 Size: 7585 AleI CACnn'nnGTG Cuts at: 1166 3959 1166 Size: 2793 4792 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4245 1184 Size: 3061 4524 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4454 4486 4454 Size: 32 7553 AlwNI CAG_nnn'CTG Cuts at: 5328 6900 5328 Size: 1572 6013 ApaLI G'TGCA_C Cuts at: 5642 6798 5642 Size: 1156 6429 ApoI r'AATT_y Cuts at: 1493 2225 2263 3577 3631 3937 4043 4836 4894 Size: 732 38 1314 54 306 106 793 58 Cuts at: 4894 5120 5272 7381 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1314 793 732 306 226 152 106 58 54 38 AseI AT'TA_AT Cuts at: 160 1032 3213 6139 160 Size: 872 2181 2926 1606 Fragments arranged by size: 2926 2181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7585 AvaI C'yCGr_G Cuts at: 1870 1943 3039 4473 4910 1870 Size: 73 1096 1434 437 4545 Fragments arranged by size: 4545 1434 1096 437 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3662 3695 3662 Size: 33 7552 BamHI G'GATC_C Cuts at: 4915 5282 4915 Size: 367 7218 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4906 6361 618 Size: 325 217 627 3119 1455 1842 Fragments arranged by size: 3119 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4904 1391 Size: 3513 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2490 4203 4537 4821 928 Size: 971 591 1713 334 284 3692 Fragments arranged by size: 3692 1713 971 591 334 284 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5776 5810 1903 Size: 34 3839 34 3678 Fragments arranged by size: 3839 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4270 4501 5477 6687 4270 Size: 231 976 1210 5168 Fragments arranged by size: 5168 1210 976 231 BclI T'GATC_A Cuts at: 1548 2362 1548 Size: 814 6771 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2030 6196 136 Size: 107 122 71 631 605 358 4166 1525 Fragments arranged by size: 4166 1525 631 605 358 122 107 71 BglII A'GATC_T Cuts at: 3067 7580 3067 Size: 4513 3072 BlpI GC'TnA_GC Cuts at: 5002 5002 Size: 7585 Bme1580I G_kGCm'C Cuts at: 1790 5646 6802 1790 Size: 3856 1156 2573 Fragments arranged by size: 3856 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5316 6274 442 Size: 416 53 83 399 24 3899 958 1753 Fragments arranged by size: 3899 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7585 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2964 6245 1239 Size: 1725 3281 2579 Fragments arranged by size: 3281 2579 1725 Bpu10I CC'TnA_GC Cuts at: 2531 2531 Size: 7585 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4189 4256 4805 5710 6575 6873 4189 Size: 67 549 905 865 298 4901 Fragments arranged by size: 4901 905 865 549 298 67 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3137 4022 6248 882 Size: 617 1638 885 2226 2219 Fragments arranged by size: 2226 2219 1638 885 617 BsaAI yAC'GTr Cuts at: 493 1204 2216 5376 493 Size: 711 1012 3160 2702 Fragments arranged by size: 3160 2702 1012 711 BsaBI GATnn'nnATC Cuts at: 4207 7579 4207 Size: 3372 4213 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5392 5774 6409 275 Size: 53 83 186 936 3859 382 635 1451 Fragments arranged by size: 3859 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2383 2566 3771 4455 6018 6416 6690 6837 7248 Size: 183 1205 684 1563 398 274 147 411 Cuts at: 7248 2383 Size: 2720 Fragments arranged by size: 2720 1563 1205 684 411 398 274 183 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2962 2992 4382 4412 4470 Size: 30 165 30 1559 30 1390 30 58 Cuts at: 4470 4500 1178 Size: 30 4263 Fragments arranged by size: 4263 1559 1390 165 58 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4427 4491 1186 Size: 3241 64 4280 Fragments arranged by size: 4280 3241 64 BseYI C'CCAG_C Cuts at: 4420 6788 7284 4420 Size: 2368 496 4721 Fragments arranged by size: 4721 2368 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2657 1163 Size: 573 921 6091 Fragments arranged by size: 6091 921 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5035 5796 6824 1540 Size: 275 186 3034 761 1028 2301 Fragments arranged by size: 3034 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4419 5646 5731 6802 4419 Size: 1227 85 1071 5202 Fragments arranged by size: 5202 1227 1071 85 BsmI GAATG_Cn' Cuts at: 2766 4628 5096 5189 2766 Size: 1862 468 93 5162 Fragments arranged by size: 5162 1862 468 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4274 4274 Size: 7585 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4419 4904 5646 5731 6802 1391 Size: 399 2629 485 742 85 1071 2174 Fragments arranged by size: 2629 2174 1071 742 485 399 85 BspEI T'CCGG_A Cuts at: 2566 4455 7248 2566 Size: 1889 2793 2903 Fragments arranged by size: 2903 2793 1889 BspHI T'CATG_A Cuts at: 5474 7204 5474 Size: 1730 5855 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4940 844 Size: 4096 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5472 1394 Size: 429 3649 3507 Fragments arranged by size: 3649 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4633 6080 6254 65 Size: 1183 2981 404 1447 174 1396 Fragments arranged by size: 2981 1447 1396 1183 404 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6229 6416 1700 Size: 110 4419 187 2869 Fragments arranged by size: 4419 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7585 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7585 BssSI C'ACGA_G Cuts at: 5645 6657 5645 Size: 1012 6573 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7585 BstBI TT'CG_AA Cuts at: 3100 6389 3100 Size: 3289 4296 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7585 BstXI CCAn_nnnn'nTGG Cuts at: 3849 3849 Size: 7585 BstYI r'GATC_y Cuts at: 1972 2600 2954 3067 3320 4489 4915 5282 5667 Size: 628 354 113 253 1169 426 367 385 Cuts at: 5667 5684 7136 7222 7234 7580 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1169 628 426 385 367 354 346 253 113 86 17 12 BstZ17I GTA'TAC Cuts at: 4652 4652 Size: 7585 Bsu36I CC'TnA_GG Cuts at: 2557 2557 Size: 7585 BtgI C'CryG_G Cuts at: 513 1065 2870 513 Size: 552 1805 5228 Fragments arranged by size: 5228 1805 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6846 Fragments arranged by size: 6846 683 56 BtsI GCAGTG_nn' Cuts at: 5101 5895 5915 5101 Size: 794 20 6771 Fragments arranged by size: 6771 794 20 ClaI AT'CG_AT Cuts at: 1554 2445 1554 Size: 891 6694 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3857 3892 529 Size: 35 3293 35 4222 Fragments arranged by size: 4222 3293 35 35 DraI TTT'AAA Cuts at: 3028 4542 4889 3028 Size: 1514 347 5724 Fragments arranged by size: 5724 1514 347 DraIII CAC_nnn'GTG Cuts at: 3016 3619 3016 Size: 603 6982 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6592 809 Size: 507 5276 1802 Fragments arranged by size: 5276 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2270 5032 5921 Size: 54 1425 50 275 18 440 2762 889 Cuts at: 5921 7550 8 Size: 1629 43 Fragments arranged by size: 2762 1629 1425 889 440 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5032 1537 Size: 275 3220 4090 Fragments arranged by size: 4090 3220 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2197 3479 3558 4245 4570 4875 5515 1353 Size: 844 1282 79 687 325 305 640 3423 Fragments arranged by size: 3423 1282 844 687 640 325 305 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4826 6172 6546 6692 387 Size: 1064 138 3237 1346 374 146 1280 Fragments arranged by size: 3237 1346 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2642 2832 2964 4728 4779 5648 6245 Size: 393 1010 190 132 1764 51 869 597 Cuts at: 6245 7032 7300 1239 Size: 787 268 1524 Fragments arranged by size: 1764 1524 1010 869 787 597 393 268 190 132 51 EcoO109I rG'GnC_Cy Cuts at: 3005 3167 3005 Size: 162 7423 EcoRI G'AATT_C Cuts at: 3577 3937 4894 5272 3577 Size: 360 957 378 5890 Fragments arranged by size: 5890 957 378 360 EcoRV GAT'ATC Cuts at: 3967 3967 Size: 7585 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3086 4865 3086 Size: 1779 5806 FspI TGC'GCA Cuts at: 3927 6091 3927 Size: 2164 5421 HaeII r_GCGC'y Cuts at: 1678 6732 1678 Size: 5054 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2347 2379 2585 2617 2813 Size: 32 135 32 775 32 206 32 196 Cuts at: 2813 2845 2962 2989 2994 3021 3134 3166 6226 Size: 32 117 27 5 27 113 32 3060 Cuts at: 6226 6258 6300 6332 1373 Size: 32 42 32 2626 Fragments arranged by size: 3060 2626 775 206 196 135 117 113 42 32 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 3196 4929 5175 669 Size: 988 281 1258 1733 246 3079 Fragments arranged by size: 3079 1733 1258 988 281 246 HindIII A'AGCT_T Cuts at: 748 2150 2780 3273 3486 3971 4495 748 Size: 1402 630 493 213 485 524 3838 Fragments arranged by size: 3838 1402 630 524 493 485 213 HpaI GTT'AAC Cuts at: 5175 5175 Size: 7585 KpnI G_GTAC'C Cuts at: 4910 4910 Size: 7585 MfeI C'AATT_G Cuts at: 4290 5184 4290 Size: 894 6691 MluI A'CGCG_T Cuts at: 4798 7461 4798 Size: 2663 4922 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2866 2898 4599 6699 6883 1305 Size: 1561 32 1701 2100 184 2007 Fragments arranged by size: 2100 2007 1701 1561 184 32 MscI TGG'CCA Cuts at: 10 64 1489 1832 2272 10 Size: 54 1425 343 440 5323 Fragments arranged by size: 5323 1425 440 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3959 5543 5902 6061 518 Size: 648 2793 1584 359 159 2042 Fragments arranged by size: 2793 2042 1584 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 3923 5001 5678 6826 7071 1606 Size: 421 1896 1078 677 1148 245 2120 Fragments arranged by size: 2120 1896 1148 1078 677 421 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7033 NdeI CA'TA_TG Cuts at: 387 387 Size: 7585 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7585 NotI GC'GGCC_GC Cuts at: 5032 5032 Size: 7585 NspI r_CATG'y Cuts at: 1132 1229 2806 3283 3604 4663 4943 6488 1132 Size: 97 1577 477 321 1059 280 1545 2229 Fragments arranged by size: 2229 1577 1545 1059 477 321 280 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7585 PciI A'CATG_T Cuts at: 2802 6484 2802 Size: 3682 3903 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7585 PmeI GTTT'AAAC Cuts at: 4889 4889 Size: 7585 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5654 5686 7167 7199 5654 Size: 32 1481 32 6040 Fragments arranged by size: 6040 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3005 3005 Size: 7585 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7585 PsiI TTA'TAA Cuts at: 2850 5155 2850 Size: 2305 5280 PstI C_TGCA'G Cuts at: 830 4263 4937 830 Size: 3433 674 3478 Fragments arranged by size: 3478 3433 674 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7585 PvuII CAG'CTG Cuts at: 3923 3923 Size: 7585 SalI G'TCGA_C Cuts at: 1936 4927 1936 Size: 2991 4594 SapI GCTCTTCn'nnn_ Cuts at: 3479 4875 3479 Size: 1396 6189 SbfI CC_TGCA'GG Cuts at: 4937 4937 Size: 7585 ScaI AGT'ACT Cuts at: 5833 7545 5833 Size: 1712 5873 SexAI A'CCwGG_T Cuts at: 3202 3202 Size: 7585 SfcI C'TryA_G Cuts at: 826 1045 3176 4259 4933 6068 6749 6940 826 Size: 219 2131 1083 674 1135 681 191 1471 Fragments arranged by size: 2131 1471 1135 1083 681 674 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2030 2030 Size: 7585 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7585 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7585 SmaI CCC'GGG Cuts at: 1872 4912 1872 Size: 3040 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2783 4168 4271 4820 5689 6590 Size: 197 926 840 1385 103 549 869 901 Cuts at: 6590 6852 820 Size: 262 1553 Fragments arranged by size: 1553 1385 926 901 869 840 549 262 197 103 SnaBI TAC'GTA Cuts at: 493 2216 5376 493 Size: 1723 3160 2702 Fragments arranged by size: 3160 2702 1723 SpeI A'CTAG_T Cuts at: 152 2909 152 Size: 2757 4828 SphI G_CATG'C Cuts at: 1132 3604 4663 4943 1132 Size: 2472 1059 280 3774 Fragments arranged by size: 3774 2472 1059 280 SspI AAT'ATT Cuts at: 5 52 2943 5509 5 Size: 47 2891 2566 2081 Fragments arranged by size: 2891 2566 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7585 StyI C'CwwG_G Cuts at: 513 1065 5024 513 Size: 552 3959 3074 Fragments arranged by size: 3959 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4215 4434 5612 5629 5782 5967 6412 Size: 1255 2635 219 1178 17 153 185 445 Cuts at: 6412 325 Size: 1498 Fragments arranged by size: 2635 1498 1255 1178 445 219 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5831 7543 96 Size: 274 80 33 51 1443 3854 1712 138 Fragments arranged by size: 3854 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7545 7577 7545 Size: 32 7553 Tth111I GACn'n_nGTC Cuts at: 1316 2016 3356 1316 Size: 700 1340 5545 Fragments arranged by size: 5545 1340 700 XbaI T'CTAG_A Cuts at: 4921 4921 Size: 7585 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3412 4384 3412 Size: 972 6613 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7585 XmaI C'CCGG_G Cuts at: 1870 4910 1870 Size: 3040 4545 XmnI GAAnn'nnTTC Cuts at: 2667 3861 4869 5714 2667 Size: 1194 1008 845 4538 Fragments arranged by size: 4538 1194 1008 845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5393 276 Size: 53 83 186 936 3859 2468 Fragments arranged by size: 3859 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfoI SrfI SwaI