Restriction map for
pFN21AA0567
(Linear) (Six-base) MAPSORT of: KIAA0567.seq Check: 7534 from: 1 to: 2880
KIAA0567.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AccI GT'mk_AC
Cuts at: 0 1406 2646 2880
Size: 1406 1240 234
Fragments arranged by size:
1406 1240 234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 827 2723 2774 2880
Size: 637 190 1896 51 106
Fragments arranged by size:
1896 637 190 106 51
AflII C'TTAA_G
Cuts at: 0 778 2880
Size: 778 2102
AflIII A'CryG_T
Cuts at: 0 797 967 1708 2793 2880
Size: 797 170 741 1085 87
Fragments arranged by size:
1085 797 741 170 87
AleI CACnn'nnGTG
Cuts at: 0 1954 2880
Size: 1954 926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2240 2880
Size: 2240 640
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2449 2481 2880
Size: 2449 32 399
Fragments arranged by size:
2449 399 32
ApoI r'AATT_y
Cuts at: 0 220 258 1572 1626 1932 2038 2831 2880
Size: 220 38 1314 54 306 106 793 49
Fragments arranged by size:
1314 793 306 220 106 54 49 38
AseI AT'TA_AT
Cuts at: 0 1208 2880
Size: 1208 1672
AvaI C'yCGr_G
Cuts at: 0 1034 2468 2880
Size: 1034 1434 412
Fragments arranged by size:
1434 1034 412
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1657 1690 2880
Size: 1657 33 1190
Fragments arranged by size:
1657 1190 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 485 2198 2532 2816 2880
Size: 485 1713 334 284 64
Fragments arranged by size:
1713 485 334 284 64
BciVI GTATCCnnnnn_n'
Cuts at: 0 2265 2496 2880
Size: 2265 231 384
Fragments arranged by size:
2265 384 231
BclI T'GATC_A
Cuts at: 0 357 2880
Size: 357 2523
BglI GCCn_nnn'nGGC
Cuts at: 0 25 2880
Size: 25 2855
BglII A'GATC_T
Cuts at: 0 1062 2880
Size: 1062 1818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 2880
Size: 959 1921
Bpu10I CC'TnA_GC
Cuts at: 0 526 2880
Size: 526 2354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2184 2251 2800 2880
Size: 2184 67 549 80
Fragments arranged by size:
2184 549 80 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 1132 2017 2880
Size: 1132 885 863
Fragments arranged by size:
1132 885 863
BsaAI yAC'GTr
Cuts at: 0 211 2880
Size: 211 2669
BsaBI GATnn'nnATC
Cuts at: 0 2202 2880
Size: 2202 678
BsaWI w'CCGG_w
Cuts at: 0 378 561 1766 2450 2880
Size: 378 183 1205 684 430
Fragments arranged by size:
1205 684 430 378 183
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 957 987 2377 2407 2465 2495 2880
Size: 957 30 1390 30 58 30 385
Fragments arranged by size:
1390 957 385 58 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2422 2486 2880
Size: 2422 64 394
Fragments arranged by size:
2422 394 64
BseYI C'CCAG_C
Cuts at: 0 2415 2880
Size: 2415 465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 2880
Size: 652 2228
BsiHKAI G_wGCw'C
Cuts at: 0 2414 2880
Size: 2414 466
BsmI GAATG_Cn'
Cuts at: 0 761 2623 2880
Size: 761 1862 257
Fragments arranged by size:
1862 761 257
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2269 2880
Size: 2269 611
Bsp1286I G_dGCh'C
Cuts at: 0 2414 2880
Size: 2414 466
BspEI T'CCGG_A
Cuts at: 0 561 2450 2880
Size: 561 1889 430
Fragments arranged by size:
1889 561 430
BsrDI GCAATG_nn'
Cuts at: 0 2224 2628 2880
Size: 2224 404 252
Fragments arranged by size:
2224 404 252
BstBI TT'CG_AA
Cuts at: 0 1095 2880
Size: 1095 1785
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1844 2880
Size: 1844 1036
BstYI r'GATC_y
Cuts at: 0 595 949 1062 1315 2484 2880
Size: 595 354 113 253 1169 396
Fragments arranged by size:
1169 595 396 354 253 113
BstZ17I GTA'TAC
Cuts at: 0 2647 2880
Size: 2647 233
Bsu36I CC'TnA_GG
Cuts at: 0 552 2880
Size: 552 2328
BtgI C'CryG_G
Cuts at: 0 865 2880
Size: 865 2015
ClaI AT'CG_AT
Cuts at: 0 440 2880
Size: 440 2440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1852 1887 2880
Size: 1852 35 993
Fragments arranged by size:
1852 993 35
DraI TTT'AAA
Cuts at: 0 1023 2537 2880
Size: 1023 1514 343
Fragments arranged by size:
1514 1023 343
DraIII CAC_nnn'GTG
Cuts at: 0 1011 1614 2880
Size: 1011 603 1266
Fragments arranged by size:
1266 1011 603
EaeI y'GGCC_r
Cuts at: 0 265 2880
Size: 265 2615
EarI CTCTTCn'nnn_
Cuts at: 0 192 1474 1553 2240 2565 2870 2880
Size: 192 1282 79 687 325 305 10
Fragments arranged by size:
1282 687 325 305 192 79 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2821 2880
Size: 2821 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 827 959 2723 2774 2880
Size: 637 190 132 1764 51 106
Fragments arranged by size:
1764 637 190 132 106 51
EcoO109I rG'GnC_Cy
Cuts at: 0 1000 1162 2880
Size: 1000 162 1718
Fragments arranged by size:
1718 1000 162
EcoRI G'AATT_C
Cuts at: 0 1572 1932 2880
Size: 1572 360 948
Fragments arranged by size:
1572 948 360
EcoRV GAT'ATC
Cuts at: 0 1962 2880
Size: 1962 918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1081 2860 2880
Size: 1081 1779 20
Fragments arranged by size:
1779 1081 20
FspI TGC'GCA
Cuts at: 0 1922 2880
Size: 1922 958
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 342 374 580 612 808 840 957 984
Size: 342 32 206 32 196 32 117 27
Cuts at: 984 989 1016 1129 1161 2880
Size: 5 27 113 32 1719
Fragments arranged by size:
1719 342 206 196 117 113 32 32
32 32 27 27 5
HincII GTy'rAC
Cuts at: 0 1191 2880
Size: 1191 1689
HindIII A'AGCT_T
Cuts at: 0 145 775 1268 1481 1966 2490 2880
Size: 145 630 493 213 485 524 390
Fragments arranged by size:
630 524 493 485 390 213 145
MfeI C'AATT_G
Cuts at: 0 2285 2880
Size: 2285 595
MluI A'CGCG_T
Cuts at: 0 2793 2880
Size: 2793 87
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 861 893 2594 2880
Size: 861 32 1701 286
Fragments arranged by size:
1701 861 286 32
MscI TGG'CCA
Cuts at: 0 267 2880
Size: 267 2613
MslI CAynn'nnrTG
Cuts at: 0 1954 2880
Size: 1954 926
MspA1I CmG'CkG
Cuts at: 0 22 1918 2880
Size: 22 1896 962
Fragments arranged by size:
1896 962 22
NspI r_CATG'y
Cuts at: 0 801 1278 1599 2658 2880
Size: 801 477 321 1059 222
Fragments arranged by size:
1059 801 477 321 222
PciI A'CATG_T
Cuts at: 0 797 2880
Size: 797 2083
PpuMI rG'GwC_Cy
Cuts at: 0 1000 2880
Size: 1000 1880
PsiI TTA'TAA
Cuts at: 0 845 2880
Size: 845 2035
PstI C_TGCA'G
Cuts at: 0 2258 2880
Size: 2258 622
PvuII CAG'CTG
Cuts at: 0 1918 2880
Size: 1918 962
SapI GCTCTTCn'nnn_
Cuts at: 0 1474 2870 2880
Size: 1474 1396 10
Fragments arranged by size:
1474 1396 10
SexAI A'CCwGG_T
Cuts at: 0 1197 2880
Size: 1197 1683
SfcI C'TryA_G
Cuts at: 0 1171 2254 2880
Size: 1171 1083 626
Fragments arranged by size:
1171 1083 626
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 25 2880
Size: 25 2855
SmlI C'TyrA_G
Cuts at: 0 778 2163 2266 2815 2880
Size: 778 1385 103 549 65
Fragments arranged by size:
1385 778 549 103 65
SnaBI TAC'GTA
Cuts at: 0 211 2880
Size: 211 2669
SpeI A'CTAG_T
Cuts at: 0 904 2880
Size: 904 1976
SphI G_CATG'C
Cuts at: 0 1599 2658 2880
Size: 1599 1059 222
Fragments arranged by size:
1599 1059 222
SspI AAT'ATT
Cuts at: 0 938 2880
Size: 938 1942
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2210 2429 2880
Size: 2210 219 451
Fragments arranged by size:
2210 451 219
Tth111I GACn'n_nGTC
Cuts at: 0 11 1351 2880
Size: 11 1340 1529
Fragments arranged by size:
1529 1340 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1407 2379 2880
Size: 1407 972 501
Fragments arranged by size:
1407 972 501
XmnI GAAnn'nnTTC
Cuts at: 0 662 1856 2864 2880
Size: 662 1194 1008 16
Fragments arranged by size:
1194 1008 662 16
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI AloI ApoI
AseI AvaI BaeI BbsI BciVI BclI BglI BglII
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BsrDI BstBI BstXI BstYI BstZ17I Bsu36I BtgI ClaI
CspCI DraI DraIII EaeI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI Hin4I HincII HindIII MfeI
MluI MmeI MscI MslI MspA1I NspI PciI PpuMI
PsiI PstI PvuII SapI SexAI SfcI SfiI SmlI
SnaBI SpeI SphI SspI TaqII Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlwNI
ApaI ApaLI AscI AsiSI AvrII BamHI BanI BanII
BbeI BbvCI BcgI BfrBI BlpI Bme1580I BmgBI BmrI
BmtI BplI BsaHI BsiEI BsiWI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BtgZI BtsI
DrdI EagI EcoICRI EcoNI FseI FspAI HaeII HpaI
KasI KpnI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfoI SgfI
SgrAI SmaI SrfI StuI StyI SwaI TatI TstI
XbaI XhoI XmaI ZraI