Restriction map for
pFN21AA0560
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI AhdI Aor51HI AscI AspEI
AvrII BbeI BbrPI BbvCI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsiEI BsmBI
BspDI BspEI BsrBI BssHII BssSI
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I FauI
FseI Hin1I HpaI KasI KspI
MfeI MluI MroI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 2046 GGTACC G^GTACC
Alw44I 2096 GTGCAC G^TGCAC
ApaI 2466 GGGCCC GGGCC^C
ApaLI 2096 GTGCAC G^TGCAC
Asp718I 2046 GGTACC G^GTACC
AspHI 2096 GTGCAC G[AT]GC[AT]^C
AspI 3142 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 12 TGCGCA TGC^GCA
BanI 2046 GGTACC G^G[CT][GA]CC
BclI 450 TGATCA T^GATCA
BsaBI 880 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1733 ACCGGA [AT]^CCGG[AT]
BsiHKAI 2096 GTGCAC G[AT]GC[AT]^C
BsiWI 1698 CGTACG C^GTACG
BsmI 2438 GAATGC GAATGC(1/-1)
BsrFI 4401 ACCGGC [GA]^CCGG[CT]
Bst1107I 4093 GTATAC GTA^TAC
BstEII 3652 GGTTACC G^GT[ACGT]ACC
BstPI 3652 GGTTACC G^GT[ACGT]ACC
BstXI 3833 CCAGGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4093 GTATAC GTA^TAC
Cfr10I 4401 ACCGGC [GA]^CCGG[CT]
DraIII 1132 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1480 CCATGG C^C[GA][CT]GG
EcoNI 2473 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3652 GGTTACC G^GT[ACGT]ACC
EcoRI 3028 GAATTC G^AATTC
EcoRV 2065 GATATC GAT^ATC
EcoT22I 4108 ATGCAT ATGCA^T
FbaI 450 TGATCA T^GATCA
FspI 12 TGCGCA TGC^GCA
HgaI 2631 GCGTC GACGC(5/10)
HindIII 3423 AAGCTT A^AGCTT
KpnI 2046 GGTACC GGTAC^C
MamI 880 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1480 CCATGG C^CATGG
NruI 3711 TCGCGA TCG^CGA
NsiI 4108 ATGCAT ATGCA^T
PflFI 3142 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2792 CTGCAG CTGCA^G
SphI 1796 GCATGC GCATG^C
SspI 1309 AATATT AAT^ATT
StuI 263 AGGCCT AGG^CCT
Tth111I 3142 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccI 475 GTAGAC GT^[AC][GT]AC
4093 GTATAC
AseI 937 ATTAAT AT^TAAT
3538 ATTAAT
AsnI 937 ATTAAT AT^TAAT
3538 ATTAAT
AvaI 1082 CTCGGG C^[CT]CG[GA]G
1598 CCCGAG
BamHI 621 GGATCC G^GATCC
1887 GGATCC
BcgI 2098 GCACATTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3702 GCATCTTATTCG
BciVI 1278 GTATCC GTATCC(6/5)
3919 GTATCC
BglII 2953 AGATCT A^GATCT
3067 AGATCT
BsoBI 1082 CTCGGG C^[CT]CG[GA]G
1598 CCCGAG
Bsp1407I 3635 TGTACA T^GTACA
4103 TGTACA
BspLU11I 3638 ACATGT A^CATGT
4100 ACATGT
BsrDI 454 CATTGC GCAATG(2/0)
2870 CATTGC
BsrGI 3635 TGTACA T^GTACA
4103 TGTACA
BstAPI 4 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3480 GCAGCTCTTGC
BstBI 2106 TTCGAA TT^CGAA
3050 TTCGAA
EcoT14I 1091 CCTTGG C^C[AT][AT]GG
1480 CCATGG
HaeII 800 GGCGCT [GA]GCGC^[CT]
2618 AGCGCC
HapII 1734 CCGG C^CGG
4402 CCGG
HincII 3391 GTTGAC GT[CT]^[GA]AC
3794 GTCAAC
HindII 3391 GTTGAC GT[CT]^[GA]AC
3794 GTCAAC
HpaII 1734 CCGG C^CGG
4402 CCGG
MspA1I 535 CAGCTG C[AC]G^C[GT]G
4272 CAGCTG
MspI 1734 CCGG C^CGG
4402 CCGG
NspV 2106 TTCGAA TT^CGAA
3050 TTCGAA
PpuMI 2084 GGGACCC [GA]G^G[AT]CC[CT]
2361 GGGTCCT
PshBI 937 ATTAAT AT^TAAT
3538 ATTAAT
PvuII 535 CAGCTG CAG^CTG
4272 CAGCTG
SapI 2650 GAAGAGC GCTCTTC(1/4)
1351 GCTCTTC
ScaI 1325 AGTACT AGT^ACT
3332 AGTACT
SfuI 2106 TTCGAA TT^CGAA
3050 TTCGAA
SmlI 3022 CTTGAG C^T[CT][GA]AG
3404 CTCAAG
SspBI 3635 TGTACA T^GTACA
4103 TGTACA
StyI 1091 CCTTGG C^C[AT][AT]GG
1480 CCATGG
XcmI 20 CCAAGAAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4370 CCACCCCTACTGTGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AvaII BalI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI Eco81I EcoO109I EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI RcaI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmnI