(Circular) (Six-base) MAPSORT of: pFN21AA0473.seq Check: 1193 from: 1 to: 7444 pFN21AA0473 7444 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4585 4585 Size: 7444 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5254 278 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2328 3668 4787 1937 Size: 391 1340 1119 4594 Fragments arranged by size: 4594 1340 1119 391 Acc65I G'GTAC_C Cuts at: 4765 4765 Size: 7444 AclI AA'CG_TT Cuts at: 1563 5571 5944 1563 Size: 4008 373 3063 Fragments arranged by size: 4008 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2957 5507 6891 7159 1632 Size: 1325 2550 1384 268 1917 Fragments arranged by size: 2550 1917 1384 1325 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7247 AflIII A'CryG_T Cuts at: 1203 6343 7320 1203 Size: 5140 977 1327 Fragments arranged by size: 5140 1327 977 AgeI A'CCGG_T Cuts at: 6275 6275 Size: 7444 AhdI GACnn_n'nnGTC Cuts at: 6173 6173 Size: 7444 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7444 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3426 4197 1184 Size: 2242 771 4431 Fragments arranged by size: 4431 2242 771 AlwNI CAG_nnn'CTG Cuts at: 4030 5187 6759 4030 Size: 1157 1572 4715 Fragments arranged by size: 4715 1572 1157 ApaLI G'TGCA_C Cuts at: 4631 5501 6657 4631 Size: 870 1156 5418 Fragments arranged by size: 5418 1156 870 ApoI r'AATT_y Cuts at: 1493 4712 4753 4979 5131 7240 1493 Size: 3219 41 226 152 2109 1697 Fragments arranged by size: 3219 2109 1697 226 152 41 AseI AT'TA_AT Cuts at: 160 1032 5998 160 Size: 872 4966 1606 Fragments arranged by size: 4966 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 AvaI C'yCGr_G Cuts at: 1870 1943 4769 1870 Size: 73 2826 4545 Fragments arranged by size: 4545 2826 73 BamHI G'GATC_C Cuts at: 3986 4033 4450 4774 5141 3986 Size: 47 417 324 367 6289 Fragments arranged by size: 6289 417 367 324 47 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 3737 4181 4765 6220 Size: 325 217 627 240 1710 444 584 1455 Cuts at: 6220 618 Size: 1842 Fragments arranged by size: 1842 1710 1455 627 584 444 325 240 217 BanII G_rGCy'C Cuts at: 1391 2052 4510 4695 4763 1391 Size: 661 2458 185 68 4072 Fragments arranged by size: 4072 2458 661 185 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3339 4359 928 Size: 971 1440 1020 4013 Fragments arranged by size: 4013 1440 1020 971 BbvCI CC'TCA_GC Cuts at: 3066 4215 3066 Size: 1149 6295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5635 5669 1903 Size: 34 3698 34 3678 Fragments arranged by size: 3698 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5336 6546 5336 Size: 1210 6234 BclI T'GATC_A Cuts at: 1548 1548 Size: 7444 BfrBI ATG'CAT Cuts at: 3041 4525 3041 Size: 1484 5960 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2401 6055 136 Size: 107 122 71 631 605 729 3654 1525 Fragments arranged by size: 3654 1525 729 631 605 122 107 71 BglII A'GATC_T Cuts at: 7439 7439 Size: 7444 BlpI GC'TnA_GC Cuts at: 4861 4861 Size: 7444 Bme1580I G_kGCm'C Cuts at: 1790 4635 5505 6661 1790 Size: 2845 870 1156 2573 Fragments arranged by size: 2845 2573 1156 870 BmgBI CAC'GTC Cuts at: 1318 2890 1318 Size: 1572 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2406 3667 4659 Size: 416 53 83 399 24 989 1261 992 Cuts at: 4659 5175 6133 442 Size: 516 958 1753 Fragments arranged by size: 1753 1261 992 989 958 516 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2022 2200 3228 3821 3933 6104 1239 Size: 783 178 1028 593 112 2171 2579 Fragments arranged by size: 2579 2171 1028 783 593 178 112 Bpu10I CC'TnA_GC Cuts at: 2943 3066 4209 4215 2943 Size: 123 1143 6 6172 Fragments arranged by size: 6172 1143 123 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2932 5569 6434 6732 2932 Size: 2637 865 298 3644 Fragments arranged by size: 3644 2637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2072 2383 4541 6107 882 Size: 617 573 311 2158 1566 2219 Fragments arranged by size: 2219 2158 1566 617 573 311 BsaAI yAC'GTr Cuts at: 493 1204 5235 493 Size: 711 4031 2702 Fragments arranged by size: 4031 2702 711 BsaBI GATnn'nnATC Cuts at: 3162 7438 3162 Size: 4276 3168 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4142 5251 5633 6268 Size: 53 83 186 936 2609 1109 382 635 Cuts at: 6268 275 Size: 1451 Fragments arranged by size: 2609 1451 1109 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3983 5877 6275 6549 6696 7107 3983 Size: 1894 398 274 147 411 4320 Fragments arranged by size: 4320 1894 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2877 2907 4538 4568 1178 Size: 30 165 30 1474 30 1631 30 4054 Fragments arranged by size: 4054 1631 1474 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3543 4225 1186 Size: 2357 682 4405 Fragments arranged by size: 4405 2357 682 BseYI C'CCAG_C Cuts at: 2051 2572 3167 3446 3790 4149 4220 4541 6647 Size: 521 595 279 344 359 71 321 2106 Cuts at: 6647 7143 2051 Size: 496 2352 Fragments arranged by size: 2352 2106 595 521 496 359 344 321 279 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2112 2402 3133 3621 1163 Size: 573 376 290 731 488 4986 Fragments arranged by size: 4986 731 573 488 376 290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4894 5655 6683 1540 Size: 275 186 2893 761 1028 2301 Fragments arranged by size: 2893 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3763 4635 4695 5505 5590 6661 3763 Size: 872 60 810 85 1071 4546 Fragments arranged by size: 4546 1071 872 810 85 60 BsmI GAATG_Cn' Cuts at: 2390 4955 5048 2390 Size: 2565 93 4786 Fragments arranged by size: 4786 2565 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2880 3717 4149 2880 Size: 837 432 6175 Fragments arranged by size: 6175 837 432 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 3763 4510 4635 4695 4763 5505 Size: 399 262 1711 747 125 60 68 742 Cuts at: 5505 5590 6661 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1711 1071 747 742 399 262 125 85 68 60 BspEI T'CCGG_A Cuts at: 7107 7107 Size: 7444 BspHI T'CATG_A Cuts at: 5333 7063 5333 Size: 1730 5714 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3670 4585 4799 844 Size: 2826 915 214 3489 Fragments arranged by size: 3489 2826 915 214 BsrBI CCG'CTC Cuts at: 1394 1823 3690 5331 1394 Size: 429 1867 1641 3507 Fragments arranged by size: 3507 1867 1641 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3415 3528 5939 6113 65 Size: 1183 2167 113 2411 174 1396 Fragments arranged by size: 2411 2167 1396 1183 174 113 BsrFI r'CCGG_y Cuts at: 1700 1810 2931 6088 6275 1700 Size: 110 1121 3157 187 2869 Fragments arranged by size: 3157 2869 1121 187 110 BsrGI T'GTAC_A Cuts at: 96 4608 96 Size: 4512 2932 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7444 BssSI C'ACGA_G Cuts at: 5504 6516 5504 Size: 1012 6432 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7444 BstBI TT'CG_AA Cuts at: 2287 6248 2287 Size: 3961 3483 BstEII G'GTnAC_C Cuts at: 1184 2947 1184 Size: 1763 5681 BstXI CCAn_nnnn'nTGG Cuts at: 4447 4447 Size: 7444 BstYI r'GATC_y Cuts at: 1972 3986 4033 4450 4774 5141 5526 5543 6995 Size: 2014 47 417 324 367 385 17 1452 Cuts at: 6995 7081 7093 7439 1972 Size: 86 12 346 1977 Fragments arranged by size: 2014 1977 1452 417 385 367 346 324 86 47 17 12 BstZ17I GTA'TAC Cuts at: 2329 2329 Size: 7444 Bsu36I CC'TnA_GG Cuts at: 3214 3486 3214 Size: 272 7172 BtgI C'CryG_G Cuts at: 513 1065 3134 513 Size: 552 2069 4823 Fragments arranged by size: 4823 2069 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6705 Fragments arranged by size: 6705 683 56 BtsI GCAGTG_nn' Cuts at: 2491 4960 5754 5774 2491 Size: 2469 794 20 4161 Fragments arranged by size: 4161 2469 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7409 DraI TTT'AAA Cuts at: 3015 4748 3015 Size: 1733 5711 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6451 809 Size: 507 5135 1802 Fragments arranged by size: 5135 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4437 4891 5780 Size: 54 1425 50 275 18 2607 454 889 Cuts at: 5780 7409 8 Size: 1629 43 Fragments arranged by size: 2607 1629 1425 889 454 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4891 1537 Size: 275 3079 4090 Fragments arranged by size: 4090 3079 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3182 3187 5374 1353 Size: 1829 5 2187 3423 Fragments arranged by size: 3423 2187 1829 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3531 6031 6405 6551 387 Size: 1064 138 1942 2500 374 146 1280 Fragments arranged by size: 2500 1942 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4693 4693 Size: 7444 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2022 2200 2957 3228 3821 3933 5507 Size: 393 390 178 757 271 593 112 1574 Cuts at: 5507 6104 6891 7159 1239 Size: 597 787 268 1524 Fragments arranged by size: 1574 1524 787 757 597 593 393 390 271 268 178 112 EcoO109I rG'GnC_Cy Cuts at: 4400 4400 Size: 7444 EcoRI G'AATT_C Cuts at: 4753 5131 4753 Size: 378 7066 EcoRV GAT'ATC Cuts at: 3238 3238 Size: 7444 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2140 3870 2140 Size: 1730 5714 FspI TGC'GCA Cuts at: 5950 5950 Size: 7444 HaeII r_GCGC'y Cuts at: 1678 6591 1678 Size: 4913 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3293 3325 4018 4050 4346 Size: 32 135 32 1721 32 693 32 296 Cuts at: 4346 4378 4691 4723 6085 6117 6159 6191 1373 Size: 32 313 32 1362 32 42 32 2626 Fragments arranged by size: 2626 1721 1362 693 313 296 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2254 2919 3678 4788 5034 669 Size: 988 281 316 665 759 1110 246 3079 Fragments arranged by size: 3079 1110 988 759 665 316 281 246 HindIII A'AGCT_T Cuts at: 748 2290 748 Size: 1542 5902 HpaI GTT'AAC Cuts at: 5034 5034 Size: 7444 KpnI G_GTAC'C Cuts at: 4769 4769 Size: 7444 MfeI C'AATT_G Cuts at: 5043 5043 Size: 7444 MluI A'CGCG_T Cuts at: 7320 7320 Size: 7444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2420 3740 6558 6742 1305 Size: 1115 1320 2818 184 2007 Fragments arranged by size: 2818 2007 1320 1115 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4439 10 Size: 54 1425 343 2607 3015 Fragments arranged by size: 3015 2607 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 3408 3654 5402 5761 5920 518 Size: 648 1710 532 246 1748 359 159 2042 Fragments arranged by size: 2042 1748 1710 648 532 359 246 159 MspA1I CmG'CkG Cuts at: 1606 3548 3605 3968 4343 4860 5537 6685 6930 Size: 1942 57 363 375 517 677 1148 245 Cuts at: 6930 1606 Size: 2120 Fragments arranged by size: 2120 1942 1148 677 517 375 363 245 57 NaeI GCC'GGC Cuts at: 2933 2933 Size: 7444 NcoI C'CATG_G Cuts at: 513 1065 3134 513 Size: 552 2069 4823 Fragments arranged by size: 4823 2069 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7444 NgoMIV G'CCGG_C Cuts at: 2931 2931 Size: 7444 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7444 NotI GC'GGCC_GC Cuts at: 4891 4891 Size: 7444 NsiI A_TGCA'T Cuts at: 3043 4527 3043 Size: 1484 5960 NspI r_CATG'y Cuts at: 1132 1229 3045 3872 3932 4228 4802 6347 1132 Size: 97 1816 827 60 296 574 1545 2229 Fragments arranged by size: 2229 1816 1545 827 574 296 97 60 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7444 PciI A'CATG_T Cuts at: 6343 6343 Size: 7444 PflMI CCAn_nnn'nTGG Cuts at: 1108 2497 2609 4030 4156 1108 Size: 1389 112 1421 126 4396 Fragments arranged by size: 4396 1421 1389 126 112 PmeI GTTT'AAAC Cuts at: 4748 4748 Size: 7444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5513 5545 7026 7058 5513 Size: 32 1481 32 5899 Fragments arranged by size: 5899 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3664 1613 Size: 2051 5393 PsiI TTA'TAA Cuts at: 5014 5014 Size: 7444 PstI C_TGCA'G Cuts at: 830 2466 4796 830 Size: 1636 2330 3478 Fragments arranged by size: 3478 2330 1636 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7444 PvuII CAG'CTG Cuts at: 3605 3968 4343 3605 Size: 363 375 6706 Fragments arranged by size: 6706 375 363 SacI G_AGCT'C Cuts at: 4695 4695 Size: 7444 SalI G'TCGA_C Cuts at: 1936 4786 1936 Size: 2850 4594 SbfI CC_TGCA'GG Cuts at: 4796 4796 Size: 7444 ScaI AGT'ACT Cuts at: 5692 7404 5692 Size: 1712 5732 SexAI A'CCwGG_T Cuts at: 4578 4578 Size: 7444 SfcI C'TryA_G Cuts at: 826 1045 2462 2936 3098 4792 5927 6608 6799 Size: 219 1417 474 162 1694 1135 681 191 Cuts at: 6799 826 Size: 1471 Fragments arranged by size: 1694 1471 1417 1135 681 474 219 191 162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7444 SmaI CCC'GGG Cuts at: 1872 4771 1872 Size: 2899 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2911 5548 6449 6711 820 Size: 197 926 968 2637 901 262 1553 Fragments arranged by size: 2637 1553 968 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5235 493 Size: 4742 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7444 SphI G_CATG'C Cuts at: 1132 3932 4228 4802 1132 Size: 2800 296 574 3774 Fragments arranged by size: 3774 2800 574 296 SspI AAT'ATT Cuts at: 5 52 5368 5 Size: 47 5316 2081 Fragments arranged by size: 5316 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7444 StyI C'CwwG_G Cuts at: 513 1065 3134 4048 4440 4723 4883 513 Size: 552 2069 914 392 283 160 3074 Fragments arranged by size: 3074 2069 914 552 392 283 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 4022 4094 4148 4650 5471 5488 Size: 1255 2049 393 72 54 502 821 17 Cuts at: 5488 5641 5826 6271 325 Size: 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 821 502 445 393 185 153 72 54 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2773 3865 4608 Size: 274 80 33 51 1443 796 1092 743 Cuts at: 4608 5690 7402 96 Size: 1082 1712 138 Fragments arranged by size: 1712 1443 1092 1082 796 743 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2704 2736 2949 2981 3835 3867 4538 4570 7404 Size: 32 213 32 854 32 671 32 2834 Cuts at: 7404 7436 2704 Size: 32 2712 Fragments arranged by size: 2834 2712 854 671 213 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7444 XbaI T'CTAG_A Cuts at: 2153 4780 2153 Size: 2627 4817 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2498 4157 4298 2498 Size: 1659 141 5644 Fragments arranged by size: 5644 1659 141 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7444 XmaI C'CCGG_G Cuts at: 1870 4769 1870 Size: 2899 4545 XmnI GAAnn'nnTTC Cuts at: 3320 3853 5573 3320 Size: 533 1720 5191 Fragments arranged by size: 5191 1720 533 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5252 276 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI DraIII EcoNI FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI