Restriction map for
pFN21AA0473
(Linear) (Six-base) MAPSORT of: KIAA0473.seq Check: 4679 from: 1 to: 2739
KIAA0473.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2580 2739
Size: 2580 159
AccI GT'mk_AC
Cuts at: 0 323 1663 2739
Size: 323 1340 1076
Fragments arranged by size:
1340 1076 323
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 2739
Size: 952 1787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1421 2192 2739
Size: 1421 771 547
Fragments arranged by size:
1421 771 547
AlwNI CAG_nnn'CTG
Cuts at: 0 2025 2739
Size: 2025 714
ApaLI G'TGCA_C
Cuts at: 0 2626 2739
Size: 2626 113
ApoI r'AATT_y
Cuts at: 0 2707 2739
Size: 2707 32
BamHI G'GATC_C
Cuts at: 0 1981 2028 2445 2739
Size: 1981 47 417 294
Fragments arranged by size:
1981 417 294 47
BanI G'GyrC_C
Cuts at: 0 22 1732 2176 2739
Size: 22 1710 444 563
Fragments arranged by size:
1710 563 444 22
BanII G_rGCy'C
Cuts at: 0 47 2505 2690 2739
Size: 47 2458 185 49
Fragments arranged by size:
2458 185 49 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 1334 2354 2739
Size: 1334 1020 385
Fragments arranged by size:
1334 1020 385
BbvCI CC'TCA_GC
Cuts at: 0 1061 2210 2739
Size: 1061 1149 529
Fragments arranged by size:
1149 1061 529
BfrBI ATG'CAT
Cuts at: 0 1036 2520 2739
Size: 1036 1484 219
Fragments arranged by size:
1484 1036 219
BglI GCCn_nnn'nGGC
Cuts at: 0 396 2739
Size: 396 2343
Bme1580I G_kGCm'C
Cuts at: 0 2630 2739
Size: 2630 109
BmgBI CAC'GTC
Cuts at: 0 885 2739
Size: 885 1854
BmrI ACTGGGnnnn_n'
Cuts at: 0 401 1662 2654 2739
Size: 401 1261 992 85
Fragments arranged by size:
1261 992 401 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 195 1223 1816 1928 2739
Size: 17 178 1028 593 112 811
Fragments arranged by size:
1028 811 593 178 112 17
Bpu10I CC'TnA_GC
Cuts at: 0 938 1061 2204 2210 2739
Size: 938 123 1143 6 529
Fragments arranged by size:
1143 938 529 123 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 2739
Size: 927 1812
BsaI GGTCTCn'nnnn_
Cuts at: 0 67 378 2536 2739
Size: 67 311 2158 203
Fragments arranged by size:
2158 311 203 67
BsaBI GATnn'nnATC
Cuts at: 0 1157 2739
Size: 1157 1582
BsaHI Gr'CG_yC
Cuts at: 0 2137 2739
Size: 2137 602
BsaWI w'CCGG_w
Cuts at: 0 1978 2739
Size: 1978 761
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 872 902 2533 2563 2739
Size: 872 30 1631 30 176
Fragments arranged by size:
1631 872 176 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1538 2220 2739
Size: 1538 682 519
Fragments arranged by size:
1538 682 519
BseYI C'CCAG_C
Cuts at: 0 46 567 1162 1441 1785 2144 2215 2536
Size: 46 521 595 279 344 359 71 321
Cuts at: 2536 2739
Size: 203
Fragments arranged by size:
595 521 359 344 321 279 203 71
46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 397 1128 1616 2739
Size: 107 290 731 488 1123
Fragments arranged by size:
1123 731 488 290 107
BsiHKAI G_wGCw'C
Cuts at: 0 1758 2630 2690 2739
Size: 1758 872 60 49
Fragments arranged by size:
1758 872 60 49
BsmI GAATG_Cn'
Cuts at: 0 385 2739
Size: 385 2354
BsmBI CGTCTCn'nnnn_
Cuts at: 0 875 1712 2144 2739
Size: 875 837 432 595
Fragments arranged by size:
875 837 595 432
Bsp1286I G_dGCh'C
Cuts at: 0 47 1758 2505 2630 2690 2739
Size: 47 1711 747 125 60 49
Fragments arranged by size:
1711 747 125 60 49 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1665 2580 2739
Size: 1665 915 159
Fragments arranged by size:
1665 915 159
BsrBI CCG'CTC
Cuts at: 0 1685 2739
Size: 1685 1054
BsrDI GCAATG_nn'
Cuts at: 0 1410 1523 2739
Size: 1410 113 1216
Fragments arranged by size:
1410 1216 113
BsrFI r'CCGG_y
Cuts at: 0 926 2739
Size: 926 1813
BsrGI T'GTAC_A
Cuts at: 0 2603 2739
Size: 2603 136
BstBI TT'CG_AA
Cuts at: 0 282 2739
Size: 282 2457
BstEII G'GTnAC_C
Cuts at: 0 942 2739
Size: 942 1797
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2442 2739
Size: 2442 297
BstYI r'GATC_y
Cuts at: 0 1981 2028 2445 2739
Size: 1981 47 417 294
Fragments arranged by size:
1981 417 294 47
BstZ17I GTA'TAC
Cuts at: 0 324 2739
Size: 324 2415
Bsu36I CC'TnA_GG
Cuts at: 0 1209 1481 2739
Size: 1209 272 1258
Fragments arranged by size:
1258 1209 272
BtgI C'CryG_G
Cuts at: 0 1129 2739
Size: 1129 1610
BtsI GCAGTG_nn'
Cuts at: 0 486 2739
Size: 486 2253
DraI TTT'AAA
Cuts at: 0 1010 2739
Size: 1010 1729
EaeI y'GGCC_r
Cuts at: 0 2432 2739
Size: 2432 307
EarI CTCTTCn'nnn_
Cuts at: 0 1177 1182 2739
Size: 1177 5 1557
Fragments arranged by size:
1557 1177 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1526 2739
Size: 1526 1213
EcoICRI GAG'CTC
Cuts at: 0 2688 2739
Size: 2688 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 195 952 1223 1816 1928 2739
Size: 17 178 757 271 593 112 811
Fragments arranged by size:
811 757 593 271 178 112 17
EcoO109I rG'GnC_Cy
Cuts at: 0 2395 2739
Size: 2395 344
EcoRV GAT'ATC
Cuts at: 0 1233 2739
Size: 1233 1506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 135 1865 2739
Size: 135 1730 874
Fragments arranged by size:
1730 874 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1288 1320 2013 2045 2341 2373 2686 2718
Size: 1288 32 693 32 296 32 313 32
Cuts at: 2718 2739
Size: 21
Fragments arranged by size:
1288 693 313 296 32 32 32 32
21
HincII GTy'rAC
Cuts at: 0 249 914 1673 2739
Size: 249 665 759 1066
Fragments arranged by size:
1066 759 665 249
HindIII A'AGCT_T
Cuts at: 0 285 2739
Size: 285 2454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1735 2739
Size: 415 1320 1004
Fragments arranged by size:
1320 1004 415
MscI TGG'CCA
Cuts at: 0 2434 2739
Size: 2434 305
MslI CAynn'nnrTG
Cuts at: 0 871 1403 1649 2739
Size: 871 532 246 1090
Fragments arranged by size:
1090 871 532 246
MspA1I CmG'CkG
Cuts at: 0 1543 1600 1963 2338 2739
Size: 1543 57 363 375 401
Fragments arranged by size:
1543 401 375 363 57
NaeI GCC'GGC
Cuts at: 0 928 2739
Size: 928 1811
NcoI C'CATG_G
Cuts at: 0 1129 2739
Size: 1129 1610
NgoMIV G'CCGG_C
Cuts at: 0 926 2739
Size: 926 1813
NsiI A_TGCA'T
Cuts at: 0 1038 2522 2739
Size: 1038 1484 217
Fragments arranged by size:
1484 1038 217
NspI r_CATG'y
Cuts at: 0 1040 1867 1927 2223 2739
Size: 1040 827 60 296 516
Fragments arranged by size:
1040 827 516 296 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 492 604 2025 2151 2739
Size: 492 112 1421 126 588
Fragments arranged by size:
1421 588 492 126 112
PshAI GACnn'nnGTC
Cuts at: 0 1659 2739
Size: 1659 1080
PstI C_TGCA'G
Cuts at: 0 461 2739
Size: 461 2278
PvuII CAG'CTG
Cuts at: 0 1600 1963 2338 2739
Size: 1600 363 375 401
Fragments arranged by size:
1600 401 375 363
SacI G_AGCT'C
Cuts at: 0 2690 2739
Size: 2690 49
SexAI A'CCwGG_T
Cuts at: 0 2573 2739
Size: 2573 166
SfcI C'TryA_G
Cuts at: 0 457 931 1093 2739
Size: 457 474 162 1646
Fragments arranged by size:
1646 474 457 162
SmlI C'TyrA_G
Cuts at: 0 906 2739
Size: 906 1833
SphI G_CATG'C
Cuts at: 0 1927 2223 2739
Size: 1927 296 516
Fragments arranged by size:
1927 516 296
StyI C'CwwG_G
Cuts at: 0 1129 2043 2435 2718 2739
Size: 1129 914 392 283 21
Fragments arranged by size:
1129 914 392 283 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1624 2017 2089 2143 2645 2739
Size: 1624 393 72 54 502 94
Fragments arranged by size:
1624 502 393 94 72 54
TatI w'GTAC_w
Cuts at: 0 768 1860 2603 2739
Size: 768 1092 743 136
Fragments arranged by size:
1092 768 743 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 699 731 944 976 1830 1862 2533 2565
Size: 699 32 213 32 854 32 671 32
Cuts at: 2565 2739
Size: 174
Fragments arranged by size:
854 699 671 213 174 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 148 2739
Size: 148 2591
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 493 2152 2293 2739
Size: 493 1659 141 446
Fragments arranged by size:
1659 493 446 141
XmnI GAAnn'nnTTC
Cuts at: 0 1315 1848 2739
Size: 1315 533 891
Fragments arranged by size:
1315 891 533
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaLI ApoI BamHI
BanI BanII BbsI BbvCI BfrBI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI DraI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HincII
HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PflMI PshAI PstI PvuII SacI SexAI
SfcI SmlI SphI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BclI BglII BlpI BmtI
BplI BsaAI BsiEI BsiWI BspEI BspHI BssHII BssSI
BstAPI BtgZI ClaI CspCI DraIII DrdI EagI EcoNI
EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XhoI
XmaI ZraI