(Circular) (Six-base) MAPSORT of: pFN21AA0411.seq Check: 2849 from: 1 to: 7930 pFN21AA0411 7930 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5740 278 Size: 53 83 186 936 4204 2468 Fragments arranged by size: 4204 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4748 5273 1937 Size: 2811 525 4594 Fragments arranged by size: 4594 2811 525 Acc65I G'GTAC_C Cuts at: 5251 5251 Size: 7930 AclI AA'CG_TT Cuts at: 1563 6057 6430 1563 Size: 4494 373 3063 Fragments arranged by size: 4494 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2486 2567 3538 4946 5993 7377 7645 1632 Size: 854 81 971 1408 1047 1384 268 1917 Fragments arranged by size: 1917 1408 1384 1047 971 854 268 81 AfeI AGC'GCT Cuts at: 2222 3043 4690 2222 Size: 821 1647 5462 Fragments arranged by size: 5462 1647 821 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7733 AflIII A'CryG_T Cuts at: 1203 2508 2964 6829 7806 1203 Size: 1305 456 3865 977 1327 Fragments arranged by size: 3865 1327 1305 977 456 AgeI A'CCGG_T Cuts at: 5130 6761 5130 Size: 1631 6299 AhdI GACnn_n'nnGTC Cuts at: 3949 4438 4789 6659 3949 Size: 489 351 1870 5220 Fragments arranged by size: 5220 1870 489 351 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7930 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2624 4862 1184 Size: 1440 2238 4252 Fragments arranged by size: 4252 2238 1440 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2243 2275 2561 2593 2243 Size: 32 286 32 7580 Fragments arranged by size: 7580 286 32 32 AlwNI CAG_nnn'CTG Cuts at: 3403 3662 5673 7245 3403 Size: 259 2011 1572 4088 Fragments arranged by size: 4088 2011 1572 259 ApaI G_GGCC'C Cuts at: 4574 4574 Size: 7930 ApaLI G'TGCA_C Cuts at: 3770 5987 7143 3770 Size: 2217 1156 4557 Fragments arranged by size: 4557 2217 1156 ApoI r'AATT_y Cuts at: 1493 3329 3595 5239 5465 5617 7726 1493 Size: 1836 266 1644 226 152 2109 1697 Fragments arranged by size: 2109 1836 1697 1644 266 226 152 AseI AT'TA_AT Cuts at: 160 1032 6484 160 Size: 872 5452 1606 Fragments arranged by size: 5452 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7930 AvaI C'yCGr_G Cuts at: 1870 1943 2186 2247 4023 4560 4593 4638 4865 Size: 73 243 61 1776 537 33 45 227 Cuts at: 4865 5152 5255 1870 Size: 287 103 4545 Fragments arranged by size: 4545 1776 537 287 243 227 103 73 61 45 33 AvrII C'CTAG_G Cuts at: 4777 4777 Size: 7930 BamHI G'GATC_C Cuts at: 3883 5260 5627 3883 Size: 1377 367 6186 Fragments arranged by size: 6186 1377 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3431 4891 5078 5222 5251 Size: 325 217 627 1644 1460 187 144 29 Cuts at: 5251 6706 618 Size: 1455 1842 Fragments arranged by size: 1842 1644 1460 1455 627 325 217 187 144 29 BanII G_rGCy'C Cuts at: 1391 2187 2463 2484 4574 4658 4923 4959 5249 Size: 796 276 21 2090 84 265 36 290 Cuts at: 5249 1391 Size: 4072 Fragments arranged by size: 4072 2090 796 290 276 265 84 36 21 BbeI G_GCGC'C Cuts at: 4895 5082 4895 Size: 187 7743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2971 3621 4824 4835 928 Size: 971 1072 650 1203 11 4023 Fragments arranged by size: 4023 1203 1072 971 650 11 BbvCI CC'TCA_GC Cuts at: 2597 2597 Size: 7930 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4163 4197 4979 5013 6121 6155 1903 Size: 34 2226 34 782 34 1108 34 3678 Fragments arranged by size: 3678 2226 1108 782 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5822 7032 5822 Size: 1210 6720 BclI T'GATC_A Cuts at: 1548 1548 Size: 7930 BfrBI ATG'CAT Cuts at: 4056 4056 Size: 7930 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2834 5072 5076 Size: 107 122 71 631 605 1162 2238 4 Cuts at: 5076 5113 6541 136 Size: 37 1428 1525 Fragments arranged by size: 2238 1525 1428 1162 631 605 122 107 71 37 4 BglII A'GATC_T Cuts at: 7925 7925 Size: 7930 BlpI GC'TnA_GC Cuts at: 2217 3045 5206 5347 2217 Size: 828 2161 141 4800 Fragments arranged by size: 4800 2161 828 141 Bme1580I G_kGCm'C Cuts at: 1790 3774 4574 4737 5225 5991 7147 1790 Size: 1984 800 163 488 766 1156 2573 Fragments arranged by size: 2573 1984 1156 800 766 488 163 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7930 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3853 5661 6619 Size: 416 53 83 399 24 2436 1808 958 Cuts at: 6619 442 Size: 1753 Fragments arranged by size: 2436 1808 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7930 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2242 4474 4860 2242 Size: 2232 386 5312 Fragments arranged by size: 5312 2232 386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2135 2225 2897 3521 3770 4083 4421 Size: 890 6 90 672 624 249 313 338 Cuts at: 4421 4817 4937 6590 1239 Size: 396 120 1653 2579 Fragments arranged by size: 2579 1653 890 672 624 396 338 313 249 120 90 6 Bpu10I CC'TnA_GC Cuts at: 2597 4364 2597 Size: 1767 6163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2978 3095 3377 6055 6920 7218 2978 Size: 117 282 2678 865 298 3690 Fragments arranged by size: 3690 2678 865 298 282 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2331 2586 2873 3248 6593 882 Size: 617 832 255 287 375 3345 2219 Fragments arranged by size: 3345 2219 832 617 375 287 255 BsaAI yAC'GTr Cuts at: 493 1204 5721 493 Size: 711 4517 2702 Fragments arranged by size: 4517 2702 711 BsaBI GATnn'nnATC Cuts at: 7924 7924 Size: 7930 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4528 4892 5079 5177 Size: 53 83 186 936 2995 364 187 98 Cuts at: 5177 5737 6119 6754 275 Size: 560 382 635 1451 Fragments arranged by size: 2995 1451 936 635 560 382 364 187 186 98 83 53 BsaWI w'CCGG_w Cuts at: 2847 5130 6363 6761 7035 7182 7593 2847 Size: 2283 1233 398 274 147 411 3184 Fragments arranged by size: 3184 2283 1233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2243 2273 2561 2591 3839 Size: 30 165 30 840 30 288 30 1248 Cuts at: 3839 3869 4259 4289 1178 Size: 30 390 30 4819 Fragments arranged by size: 4819 1248 840 390 288 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2218 2270 2471 2582 3140 3301 5014 1186 Size: 1032 52 201 111 558 161 1713 4102 Fragments arranged by size: 4102 1713 1032 558 201 161 111 52 BseYI C'CCAG_C Cuts at: 2631 3757 4016 4574 4610 5082 7133 7629 2631 Size: 1126 259 558 36 472 2051 496 2932 Fragments arranged by size: 2932 2051 1126 558 496 472 259 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4832 1163 Size: 573 3096 4261 Fragments arranged by size: 4261 3096 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3224 5117 5172 5380 6141 7169 Size: 275 186 1223 1893 55 208 761 1028 Cuts at: 7169 1540 Size: 2301 Fragments arranged by size: 2301 1893 1223 1028 761 275 208 186 55 BsiHKAI G_wGCw'C Cuts at: 2187 2256 2463 2484 3774 4455 4841 5991 6076 Size: 69 207 21 1290 681 386 1150 85 Cuts at: 6076 7147 2187 Size: 1071 2970 Fragments arranged by size: 2970 1290 1150 1071 681 386 207 85 69 21 BsmI GAATG_Cn' Cuts at: 2920 3431 5441 5534 2920 Size: 511 2010 93 5316 Fragments arranged by size: 5316 2010 511 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2256 2463 2484 3774 4455 4574 Size: 399 397 69 207 21 1290 681 119 Cuts at: 4574 4658 4737 4841 4923 4959 5225 5249 5991 Size: 84 79 104 82 36 266 24 742 Cuts at: 5991 6076 7147 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1290 1071 742 681 399 397 266 207 119 104 85 84 82 79 69 36 24 21 BspEI T'CCGG_A Cuts at: 2847 7593 2847 Size: 4746 3184 BspHI T'CATG_A Cuts at: 2364 2493 3822 4000 5819 7549 2364 Size: 129 1329 178 1819 1730 2745 Fragments arranged by size: 2745 1819 1730 1329 178 129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2616 4760 5285 844 Size: 1772 2144 525 3489 Fragments arranged by size: 3489 2144 1772 525 BsrBI CCG'CTC Cuts at: 1394 1823 5817 1394 Size: 429 3994 3507 Fragments arranged by size: 3994 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2043 3276 4400 6425 6599 65 Size: 1183 795 1233 1124 2025 174 1396 Fragments arranged by size: 2025 1396 1233 1183 1124 795 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2625 4284 5116 5130 6574 6761 1700 Size: 110 815 1659 832 14 1444 187 2869 Fragments arranged by size: 2869 1659 1444 832 815 187 110 14 BsrGI T'GTAC_A Cuts at: 96 4330 96 Size: 4234 3696 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7930 BssSI C'ACGA_G Cuts at: 2453 2618 4850 5990 7002 2453 Size: 165 2232 1140 1012 3381 Fragments arranged by size: 3381 2232 1140 1012 165 BstAPI GCAn_nnn'nTGC Cuts at: 794 4855 794 Size: 4061 3869 BstBI TT'CG_AA Cuts at: 6734 6734 Size: 7930 BstEII G'GTnAC_C Cuts at: 1184 2470 1184 Size: 1286 6644 BstXI CCAn_nnnn'nTGG Cuts at: 4130 4130 Size: 7930 BstYI r'GATC_y Cuts at: 1972 2073 3549 3566 3777 3883 5260 5627 6012 Size: 101 1476 17 211 106 1377 367 385 Cuts at: 6012 6029 7481 7567 7579 7925 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1476 1452 1377 385 367 346 211 106 101 86 17 17 12 Bsu36I CC'TnA_GG Cuts at: 3711 3711 Size: 7930 BtgI C'CryG_G Cuts at: 513 1065 4446 5226 513 Size: 552 3381 780 3217 Fragments arranged by size: 3381 3217 780 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4167 487 Size: 683 56 2941 4250 Fragments arranged by size: 4250 2941 683 56 BtsI GCAGTG_nn' Cuts at: 2925 4399 5446 6240 6260 2925 Size: 1474 1047 794 20 4595 Fragments arranged by size: 4595 1474 1047 794 20 ClaI AT'CG_AT Cuts at: 1554 2802 4110 1554 Size: 1248 1308 5374 Fragments arranged by size: 5374 1308 1248 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7895 DraI TTT'AAA Cuts at: 3334 3672 5234 3334 Size: 338 1562 6030 Fragments arranged by size: 6030 1562 338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3194 6937 809 Size: 507 1878 3743 1802 Fragments arranged by size: 3743 1878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3102 3897 5114 Size: 54 1425 50 275 18 1272 795 1217 Cuts at: 5114 5169 5377 6266 7895 8 Size: 55 208 889 1629 43 Fragments arranged by size: 1629 1425 1272 1217 889 795 275 208 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5114 5169 5377 1537 Size: 275 3302 55 208 4090 Fragments arranged by size: 4090 3302 275 208 55 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 2669 3839 4426 5860 1353 Size: 1070 246 1170 587 1434 3423 Fragments arranged by size: 3423 1434 1170 1070 587 246 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2153 2372 5053 5078 6517 6891 Size: 1064 138 564 219 2681 25 1439 374 Cuts at: 6891 7037 387 Size: 146 1280 Fragments arranged by size: 2681 1439 1280 1064 564 374 219 146 138 25 EcoICRI GAG'CTC Cuts at: 2185 2461 2482 2185 Size: 276 21 7633 Fragments arranged by size: 7633 276 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2135 2225 2486 2567 2897 3521 Size: 393 497 6 90 261 81 330 624 Cuts at: 3521 3538 3770 4083 4421 4817 4937 4946 5993 Size: 17 232 313 338 396 120 9 1047 Cuts at: 5993 6590 7377 7645 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1047 787 624 597 497 396 393 338 330 313 268 261 232 120 90 81 17 9 6 EcoO109I rG'GnC_Cy Cuts at: 3943 4037 4236 4564 4571 4807 4940 3943 Size: 94 199 328 7 236 133 6933 Fragments arranged by size: 6933 328 236 199 133 94 7 EcoRI G'AATT_C Cuts at: 5239 5617 5239 Size: 378 7552 EcoRV GAT'ATC Cuts at: 3649 3735 3649 Size: 86 7844 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3638 3638 Size: 7930 FspI TGC'GCA Cuts at: 6436 6436 Size: 7930 HaeII r_GCGC'y Cuts at: 1678 2224 2638 3045 4692 4724 4895 5082 7077 Size: 546 414 407 1647 32 171 187 1995 Cuts at: 7077 1678 Size: 2531 Fragments arranged by size: 2531 1995 1647 546 414 407 187 171 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 3719 3751 3935 Size: 32 135 32 530 32 1585 32 184 Cuts at: 3935 3967 4337 4369 4403 4424 4435 4456 4503 Size: 32 370 32 34 21 11 21 47 Cuts at: 4503 4535 4775 4807 6571 6603 6645 6677 1373 Size: 32 240 32 1764 32 42 32 2626 Fragments arranged by size: 2626 1764 1585 530 370 240 184 135 47 42 34 32 32 32 32 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 4120 4749 5274 5520 669 Size: 988 281 2182 629 525 246 3079 Fragments arranged by size: 3079 2182 988 629 525 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7930 HpaI GTT'AAC Cuts at: 5520 5520 Size: 7930 KasI G'GCGC_C Cuts at: 4891 5078 4891 Size: 187 7743 KpnI G_GTAC'C Cuts at: 5255 5255 Size: 7930 MfeI C'AATT_G Cuts at: 5529 5529 Size: 7930 MluI A'CGCG_T Cuts at: 7806 7806 Size: 7930 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3235 3805 4380 7044 7228 1305 Size: 1930 570 575 2664 184 2007 Fragments arranged by size: 2664 2007 1930 575 570 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3104 3899 10 Size: 54 1425 343 1272 795 4041 Fragments arranged by size: 4041 1425 1272 795 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 2492 2957 2963 3969 4128 5033 Size: 648 1197 129 465 6 1006 159 905 Cuts at: 5033 5888 6247 6406 518 Size: 855 359 159 2042 Fragments arranged by size: 2042 1197 1006 905 855 648 465 359 159 159 129 6 MspA1I CmG'CkG Cuts at: 1606 2086 2137 2864 3088 5210 5346 6023 7171 Size: 480 51 727 224 2122 136 677 1148 Cuts at: 7171 7416 1606 Size: 245 2120 Fragments arranged by size: 2122 2120 1148 727 677 480 245 224 136 51 NaeI GCC'GGC Cuts at: 4286 4286 Size: 7930 NarI GG'CG_CC Cuts at: 4892 5079 4892 Size: 187 7743 NcoI C'CATG_G Cuts at: 513 1065 5226 513 Size: 552 4161 3217 Fragments arranged by size: 4161 3217 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7930 NgoMIV G'CCGG_C Cuts at: 4284 4284 Size: 7930 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7930 NotI GC'GGCC_GC Cuts at: 5377 5377 Size: 7930 NruI TCG'CGA Cuts at: 2128 2128 Size: 7930 NsiI A_TGCA'T Cuts at: 4058 4058 Size: 7930 NspI r_CATG'y Cuts at: 1132 1229 2512 2968 3175 5288 6833 1132 Size: 97 1283 456 207 2113 1545 2229 Fragments arranged by size: 2229 2113 1545 1283 456 207 97 PasI CC'CwG_GG Cuts at: 1348 3403 1348 Size: 2055 5875 PciI A'CATG_T Cuts at: 2508 2964 6829 2508 Size: 456 3865 3609 Fragments arranged by size: 3865 3609 456 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7930 PfoI T'CCnGG_A Cuts at: 2151 2151 Size: 7930 PmeI GTTT'AAAC Cuts at: 5234 5234 Size: 7930 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2243 2275 2561 2593 5177 5209 5999 6031 7512 Size: 32 286 32 2584 32 790 32 1481 Cuts at: 7512 7544 2243 Size: 32 2629 Fragments arranged by size: 2629 2584 1481 790 286 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3943 4037 3943 Size: 94 7836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7930 PsiI TTA'TAA Cuts at: 5500 5500 Size: 7930 PspOMI G'GGCC_C Cuts at: 4570 4570 Size: 7930 PspXI vC'TCGA_Gb Cuts at: 2186 2186 Size: 7930 PstI C_TGCA'G Cuts at: 830 2136 2448 3093 3375 5282 830 Size: 1306 312 645 282 1907 3478 Fragments arranged by size: 3478 1907 1306 645 312 282 PvuI CG_AT'CG Cuts at: 2001 3224 2001 Size: 1223 6707 PvuII CAG'CTG Cuts at: 2086 2137 2864 3088 2086 Size: 51 727 224 6928 Fragments arranged by size: 6928 727 224 51 SacI G_AGCT'C Cuts at: 2187 2463 2484 2187 Size: 276 21 7633 Fragments arranged by size: 7633 276 21 SalI G'TCGA_C Cuts at: 1936 4747 5272 1936 Size: 2811 525 4594 Fragments arranged by size: 4594 2811 525 SbfI CC_TGCA'GG Cuts at: 5282 5282 Size: 7930 ScaI AGT'ACT Cuts at: 2192 2690 6178 7890 2192 Size: 498 3488 1712 2232 Fragments arranged by size: 3488 2232 1712 498 SfcI C'TryA_G Cuts at: 826 1045 2132 2444 3089 3371 5278 6413 7094 Size: 219 1087 312 645 282 1907 1135 681 Cuts at: 7094 7285 826 Size: 191 1471 Fragments arranged by size: 1907 1471 1135 1087 681 645 312 282 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 5113 5113 Size: 7930 SfoI GGC'GCC Cuts at: 4893 5080 4893 Size: 187 7743 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7930 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7930 SmaI CCC'GGG Cuts at: 1872 2249 4025 4562 4595 4640 5257 1872 Size: 377 1776 537 33 45 617 4545 Fragments arranged by size: 4545 1776 617 537 377 45 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 2993 3110 3392 4865 6034 Size: 197 926 243 807 117 282 1473 1169 Cuts at: 6034 6935 7197 820 Size: 901 262 1553 Fragments arranged by size: 1553 1473 1169 926 901 807 282 262 243 197 117 SnaBI TAC'GTA Cuts at: 493 5721 493 Size: 5228 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7930 SphI G_CATG'C Cuts at: 1132 5288 1132 Size: 4156 3774 SspI AAT'ATT Cuts at: 5 52 4076 5854 5 Size: 47 4024 1778 2081 Fragments arranged by size: 4024 2081 1778 47 StuI AGG'CCT Cuts at: 1517 4418 1517 Size: 2901 5029 StyI C'CwwG_G Cuts at: 513 1065 4777 5226 5369 513 Size: 552 3712 449 143 3074 Fragments arranged by size: 3712 3074 552 449 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5957 5974 6127 6312 6757 325 Size: 1255 4377 17 153 185 445 1498 Fragments arranged by size: 4377 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2190 2688 4320 Size: 274 80 33 51 1443 213 498 1632 Cuts at: 4320 4330 6176 7888 96 Size: 10 1846 1712 138 Fragments arranged by size: 1846 1712 1632 1443 498 274 213 138 80 51 33 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3558 3590 4259 4291 7890 7922 3558 Size: 32 669 32 3599 32 3566 Fragments arranged by size: 3599 3566 669 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3229 1316 Size: 1913 6017 XbaI T'CTAG_A Cuts at: 5266 5266 Size: 7930 XhoI C'TCGA_G Cuts at: 1943 2186 4865 1943 Size: 243 2679 5008 Fragments arranged by size: 5008 2679 243 XmaI C'CCGG_G Cuts at: 1870 2247 4023 4560 4593 4638 5255 1870 Size: 377 1776 537 33 45 617 4545 Fragments arranged by size: 4545 1776 617 537 377 45 33 XmnI GAAnn'nnTTC Cuts at: 2576 3720 5192 6059 2576 Size: 1144 1472 867 4447 Fragments arranged by size: 4447 1472 1144 867 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5738 276 Size: 53 83 186 936 4204 2468 Fragments arranged by size: 4204 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI PacI PmlI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI XcmI