Restriction map for
pFN21AA0321
(Linear) (Six-base) MAPSORT of: KIAA0321.seq Check: 8129 from: 1 to: 7617
KIAA0321.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4545 7497 7617
Size: 4545 2952 120
Fragments arranged by size:
4545 2952 120
AccI GT'mk_AC
Cuts at: 0 3021 5331 7617
Size: 3021 2310 2286
Fragments arranged by size:
3021 2310 2286
Acc65I G'GTAC_C
Cuts at: 0 5427 7617
Size: 5427 2190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 2593 3064 3178 3256 3450 4273 4319
Size: 145 2448 471 114 78 194 823 46
Cuts at: 4319 5621 6289 6470 6807 6916 6936 6985 7617
Size: 1302 668 181 337 109 20 49 632
Fragments arranged by size:
2448 1302 823 668 632 471 337 194
181 145 114 109 78 49 46 20
AfeI AGC'GCT
Cuts at: 0 2614 2742 7617
Size: 2614 128 4875
Fragments arranged by size:
4875 2614 128
AflII C'TTAA_G
Cuts at: 0 3922 6877 7617
Size: 3922 2955 740
Fragments arranged by size:
3922 2955 740
AflIII A'CryG_T
Cuts at: 0 2281 7617
Size: 2281 5336
AhdI GACnn_n'nnGTC
Cuts at: 0 440 3122 7617
Size: 440 2682 4495
Fragments arranged by size:
4495 2682 440
AleI CACnn'nnGTG
Cuts at: 0 4300 7617
Size: 4300 3317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4048 6113 7147 7617
Size: 4048 2065 1034 470
Fragments arranged by size:
4048 2065 1034 470
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2587 2619 7617
Size: 2587 32 4998
Fragments arranged by size:
4998 2587 32
AlwNI CAG_nnn'CTG
Cuts at: 0 510 1014 1167 1456 1482 1642 1686 2556
Size: 510 504 153 289 26 160 44 870
Cuts at: 2556 3279 3473 3689 4158 4956 5109 5112 5224
Size: 723 194 216 469 798 153 3 112
Cuts at: 5224 5534 5946 6263 7272 7374 7617
Size: 310 412 317 1009 102 243
Fragments arranged by size:
1009 870 798 723 510 504 469 412
317 310 289 243 216 194 160 153
153 112 102 44 26 3
ApaI G_GGCC'C
Cuts at: 0 619 1186 4287 7617
Size: 619 567 3101 3330
Fragments arranged by size:
3330 3101 619 567
ApoI r'AATT_y
Cuts at: 0 1081 2044 5386 5773 7358 7617
Size: 1081 963 3342 387 1585 259
Fragments arranged by size:
3342 1585 1081 963 387 259
AvaI C'yCGr_G
Cuts at: 0 263 358 876 1003 3825 3972 4058 4280
Size: 263 95 518 127 2822 147 86 222
Cuts at: 4280 5266 7586 7617
Size: 986 2320 31
Fragments arranged by size:
2822 2320 986 518 263 222 147 127
95 86 31
BamHI G'GATC_C
Cuts at: 0 4966 7617
Size: 4966 2651
BanI G'GyrC_C
Cuts at: 0 627 3457 4032 5417 5427 7591 7617
Size: 627 2830 575 1385 10 2164 26
Fragments arranged by size:
2830 2164 1385 627 575 26 10
BanII G_rGCy'C
Cuts at: 0 521 619 663 1186 1202 1233 1828 1858
Size: 521 98 44 523 16 31 595 30
Cuts at: 1858 2867 3308 3514 3574 3701 3823 4127 4287
Size: 1009 441 206 60 127 122 304 160
Cuts at: 4287 7617
Size: 3330
Fragments arranged by size:
3330 1009 595 523 521 441 304 206
160 127 122 98 60 44 31 30
16
BbsI GAAGACnn'nnnn_
Cuts at: 0 158 332 1052 2900 3317 7617
Size: 158 174 720 1848 417 4300
Fragments arranged by size:
4300 1848 720 417 174 158
BbvCI CC'TCA_GC
Cuts at: 0 110 1599 1799 1859 7617
Size: 110 1489 200 60 5758
Fragments arranged by size:
5758 1489 200 110 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6367 6401 7617
Size: 6367 34 1216
Fragments arranged by size:
6367 1216 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 6372 7617
Size: 6372 1245
BclI T'GATC_A
Cuts at: 0 3522 5592 7617
Size: 3522 2070 2025
Fragments arranged by size:
3522 2070 2025
BfrBI ATG'CAT
Cuts at: 0 4411 7617
Size: 4411 3206
BglI GCCn_nnn'nGGC
Cuts at: 0 1983 3640 5086 5513 7617
Size: 1983 1657 1446 427 2104
Fragments arranged by size:
2104 1983 1657 1446 427
BglII A'GATC_T
Cuts at: 0 1290 6083 6899 7617
Size: 1290 4793 816 718
Fragments arranged by size:
4793 1290 816 718
BlpI GC'TnA_GC
Cuts at: 0 778 2383 7617
Size: 778 1605 5234
Fragments arranged by size:
5234 1605 778
Bme1580I G_kGCm'C
Cuts at: 0 619 632 1186 3460 4287 5396 7563 7596
Size: 619 13 554 2274 827 1109 2167 33
Cuts at: 7596 7617
Size: 21
Fragments arranged by size:
2274 2167 1109 827 619 554 33 21
13
BmgBI CAC'GTC
Cuts at: 0 2284 2781 7617
Size: 2284 497 4836
Fragments arranged by size:
4836 2284 497
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 1551 2202 2285 2579 4585 6157 6182
Size: 277 1274 651 83 294 2006 1572 25
Cuts at: 6182 6862 7557 7617
Size: 680 695 60
Fragments arranged by size:
2006 1572 1274 695 680 651 294 277
83 60 25
BmtI G_CTAG'C
Cuts at: 0 925 7617
Size: 925 6692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 452 506 5169 6449 6880 6882 7617
Size: 452 54 4663 1280 431 2 735
Fragments arranged by size:
4663 1280 735 452 431 54 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 535 1144 1258 1267 1433 1735 2812
Size: 268 267 609 114 9 166 302 1077
Cuts at: 2812 2828 3341 3385 4480 4492 5065 5086 5310
Size: 16 513 44 1095 12 573 21 224
Cuts at: 5310 6581 7396 7593 7617
Size: 1271 815 197 24
Fragments arranged by size:
1271 1095 1077 815 609 573 513 302
268 267 224 197 166 114 44 24
21 16 12 9
Bpu10I CC'TnA_GC
Cuts at: 0 110 908 982 1457 1465 1599 1799 1859
Size: 110 798 74 475 8 134 200 60
Cuts at: 1859 2145 3214 4351 4991 6112 7617
Size: 286 1069 1137 640 1121 1505
Fragments arranged by size:
1505 1137 1121 1069 798 640 475 286
200 134 110 74 60 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 547 1372 4635 6291 7617
Size: 325 222 825 3263 1656 1326
Fragments arranged by size:
3263 1656 1326 825 325 222
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 2804 5263 6165 7617
Size: 367 2437 2459 902 1452
Fragments arranged by size:
2459 2437 1452 902 367
BsaAI yAC'GTr
Cuts at: 0 402 3036 6066 6540 7617
Size: 402 2634 3030 474 1077
Fragments arranged by size:
3030 2634 1077 474 402
BsaBI GATnn'nnATC
Cuts at: 0 5286 7617
Size: 5286 2331
BsaHI Gr'CG_yC
Cuts at: 0 5493 7617
Size: 5493 2124
BsaWI w'CCGG_w
Cuts at: 0 293 891 5430 7617
Size: 293 598 4539 2187
Fragments arranged by size:
4539 2187 598 293
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 241 271 1537 1567 1612 1642 4611 4641
Size: 241 30 1266 30 45 30 2969 30
Cuts at: 4641 7617
Size: 2976
Fragments arranged by size:
2976 2969 1266 241 45 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 376 428 544 781 1192 1620 2263 3173
Size: 376 52 116 237 411 428 643 910
Cuts at: 3173 3305 3388 3729 3858 3894 4632 5303 5644
Size: 132 83 341 129 36 738 671 341
Cuts at: 5644 5761 7321 7617
Size: 117 1560 296
Fragments arranged by size:
1560 910 738 671 643 428 411 376
341 341 296 237 132 129 117 116
83 52 36
BseYI C'CCAG_C
Cuts at: 0 494 945 1421 3208 3251 3770 3891 3994
Size: 494 451 476 1787 43 519 121 103
Cuts at: 3994 5372 5397 5794 6698 7048 7134 7617
Size: 1378 25 397 904 350 86 483
Fragments arranged by size:
1787 1378 904 519 494 483 476 451
397 350 121 103 86 43 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 775 1153 1710 2422 3195 3400 5113
Size: 410 365 378 557 712 773 205 1713
Cuts at: 5113 5547 5812 6227 6337 6484 6556 7531 7617
Size: 434 265 415 110 147 72 975 86
Fragments arranged by size:
1713 975 773 712 557 434 415 410
378 365 265 205 147 110 86 72
BsiHKAI G_wGCw'C
Cuts at: 0 470 521 1202 1334 1463 2389 2654 3514
Size: 470 51 681 132 129 926 265 860
Cuts at: 3514 3574 3823 5474 5870 7617
Size: 60 249 1651 396 1747
Fragments arranged by size:
1747 1651 926 860 681 470 396 265
249 132 129 60 51
BsmI GAATG_Cn'
Cuts at: 0 70 576 3056 6490 7617
Size: 70 506 2480 3434 1127
Fragments arranged by size:
3434 2480 1127 506 70
BsmBI CGTCTCn'nnnn_
Cuts at: 0 448 7617
Size: 448 7169
Bsp1286I G_dGCh'C
Cuts at: 0 470 521 619 632 663 1186 1202 1233
Size: 470 51 98 13 31 523 16 31
Cuts at: 1233 1334 1463 1828 1858 2389 2654 2867 3308
Size: 101 129 365 30 531 265 213 441
Cuts at: 3308 3460 3514 3574 3701 3823 4127 4287 5396
Size: 152 54 60 127 122 304 160 1109
Cuts at: 5396 5474 5870 7563 7596 7617
Size: 78 396 1693 33 21
Fragments arranged by size:
1693 1109 531 523 470 441 396 365
304 265 213 160 152 129 127 122
101 98 78 60 54 51 33 31
31 30 21 16 13
BspEI T'CCGG_A
Cuts at: 0 293 7617
Size: 293 7324
BspHI T'CATG_A
Cuts at: 0 4649 4779 5933 6461 7617
Size: 4649 130 1154 528 1156
Fragments arranged by size:
4649 1156 1154 528 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2968 4041 4545 6506 7497 7617
Size: 2968 1073 504 1961 991 120
Fragments arranged by size:
2968 1961 1073 991 504 120
BsrBI CCG'CTC
Cuts at: 0 771 912 2717 7617
Size: 771 141 1805 4900
Fragments arranged by size:
4900 1805 771 141
BsrDI GCAATG_nn'
Cuts at: 0 598 923 1738 4220 5810 5840 7617
Size: 598 325 815 2482 1590 30 1777
Fragments arranged by size:
2482 1777 1590 815 598 325 30
BsrFI r'CCGG_y
Cuts at: 0 5516 5977 7617
Size: 5516 461 1640
Fragments arranged by size:
5516 1640 461
BssSI C'ACGA_G
Cuts at: 0 5582 7617
Size: 5582 2035
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1444 4800 5459 5534 6215 7617
Size: 1444 3356 659 75 681 1402
Fragments arranged by size:
3356 1444 1402 681 659 75
BstBI TT'CG_AA
Cuts at: 0 591 7617
Size: 591 7026
BstEII G'GTnAC_C
Cuts at: 0 5851 7617
Size: 5851 1766
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2332 4915 7617
Size: 2332 2583 2702
Fragments arranged by size:
2702 2583 2332
BstYI r'GATC_y
Cuts at: 0 474 903 1290 3383 4966 5262 5736 6083
Size: 474 429 387 2093 1583 296 474 347
Cuts at: 6083 6899 7262 7617
Size: 816 363 355
Fragments arranged by size:
2093 1583 816 474 474 429 387 363
355 347 296
BstZ17I GTA'TAC
Cuts at: 0 5332 7617
Size: 5332 2285
Bsu36I CC'TnA_GG
Cuts at: 0 481 1204 1369 1787 1830 2065 6394 7617
Size: 481 723 165 418 43 235 4329 1223
Fragments arranged by size:
4329 1223 723 481 418 235 165 43
BtgI C'CryG_G
Cuts at: 0 2912 3169 7596 7617
Size: 2912 257 4427 21
Fragments arranged by size:
4427 2912 257 21
BtsI GCAGTG_nn'
Cuts at: 0 1125 2503 2715 2867 3786 4169 5155 5868
Size: 1125 1378 212 152 919 383 986 713
Cuts at: 5868 6236 6446 7617
Size: 368 210 1171
Fragments arranged by size:
1378 1171 1125 986 919 713 383 368
212 210 152
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 7479 7514 7617
Size: 7479 35 103
Fragments arranged by size:
7479 103 35
DraIII CAC_nnn'GTG
Cuts at: 0 388 1329 2223 3378 4826 7617
Size: 388 941 894 1155 1448 2791
Fragments arranged by size:
2791 1448 1155 941 894 388
DrdI GACnn_nn'nnGTC
Cuts at: 0 4504 7617
Size: 4504 3113
EaeI y'GGCC_r
Cuts at: 0 825 1667 2334 2424 2712 4020 4265 4871
Size: 825 842 667 90 288 1308 245 606
Cuts at: 4871 4889 5514 5979 6521 7617
Size: 18 625 465 542 1096
Fragments arranged by size:
1308 1096 842 825 667 625 606 542
465 288 245 90 18
EarI CTCTTCn'nnn_
Cuts at: 0 1657 1816 3579 4685 4767 6496 6572 7617
Size: 1657 159 1763 1106 82 1729 76 1045
Fragments arranged by size:
1763 1729 1657 1106 1045 159 82 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2287 4561 7352 7617
Size: 2287 2274 2791 265
Fragments arranged by size:
2791 2287 2274 265
EcoICRI GAG'CTC
Cuts at: 0 519 1200 3512 3572 3821 7617
Size: 519 681 2312 60 249 3796
Fragments arranged by size:
3796 2312 681 519 249 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 268 535 1144 1258 1267 1433 1735
Size: 145 123 267 609 114 9 166 302
Cuts at: 1735 2593 2812 2828 3064 3178 3256 3341 3385
Size: 858 219 16 236 114 78 85 44
Cuts at: 3385 3450 4273 4319 4480 4492 5065 5086 5310
Size: 65 823 46 161 12 573 21 224
Cuts at: 5310 5621 6289 6470 6581 6807 6916 6936 6985
Size: 311 668 181 111 226 109 20 49
Cuts at: 6985 7396 7593 7617
Size: 411 197 24
Fragments arranged by size:
858 823 668 609 573 411 311 302
267 236 226 224 219 197 181 166
161 145 123 114 114 111 109 85
78 65 49 46 44 24 21 20
16 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 526 718 763 1372 1585 2064 2881 3217
Size: 526 192 45 609 213 479 817 336
Cuts at: 3217 3873 7387 7617
Size: 656 3514 230
Fragments arranged by size:
3514 817 656 609 526 479 336 230
213 192 45
EcoO109I rG'GnC_Cy
Cuts at: 0 507 615 616 807 1088 1175 1182 1242
Size: 507 108 1 191 281 87 7 60
Cuts at: 1242 1416 1590 1841 1975 2057 3163 3267 4284
Size: 174 174 251 134 82 1106 104 1017
Cuts at: 4284 4635 5790 5889 6353 7052 7380 7617
Size: 351 1155 99 464 699 328 237
Fragments arranged by size:
1155 1106 1017 699 507 464 351 328
281 251 237 191 174 174 134 108
104 99 87 82 60 7 1
EcoRI G'AATT_C
Cuts at: 0 1081 7358 7617
Size: 1081 6277 259
Fragments arranged by size:
6277 1081 259
EcoRV GAT'ATC
Cuts at: 0 135 7617
Size: 135 7482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 175 6430 7617
Size: 175 6255 1187
Fragments arranged by size:
6255 1187 175
HaeII r_GCGC'y
Cuts at: 0 2616 2744 4074 4957 5801 5957 7617
Size: 2616 128 1330 883 844 156 1660
Fragments arranged by size:
2616 1660 1330 883 844 156 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 426 458 1309 1341 1458 1490 1567 1582
Size: 426 32 851 32 117 32 77 15
Cuts at: 1582 1599 1612 1614 1644 2801 2833 3108 3140
Size: 17 13 2 30 1157 32 275 32
Cuts at: 3140 3375 3407 4624 4656 5200 5232 5721 5753
Size: 235 32 1217 32 544 32 489 32
Cuts at: 5753 7617
Size: 1864
Fragments arranged by size:
1864 1217 1157 851 544 489 426 275
235 117 77 32 32 32 32 32
32 32 32 32 30 17 15 13
2
HincII GTy'rAC
Cuts at: 0 1678 3022 7617
Size: 1678 1344 4595
Fragments arranged by size:
4595 1678 1344
HindIII A'AGCT_T
Cuts at: 0 301 7617
Size: 301 7316
KpnI G_GTAC'C
Cuts at: 0 5431 7617
Size: 5431 2186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2290 4959 5097 6648 6709 7617
Size: 2290 2669 138 1551 61 908
Fragments arranged by size:
2669 2290 1551 908 138 61
MscI TGG'CCA
Cuts at: 0 827 4267 4873 4891 6523 7617
Size: 827 3440 606 18 1632 1094
Fragments arranged by size:
3440 1632 1094 827 606 18
MslI CAynn'nnrTG
Cuts at: 0 99 833 2479 2821 4300 4654 4913 5068
Size: 99 734 1646 342 1479 354 259 155
Cuts at: 5068 7108 7403 7617
Size: 2040 295 214
Fragments arranged by size:
2040 1646 1479 734 354 342 295 259
214 155 99
MspA1I CmG'CkG
Cuts at: 0 782 3686 5052 5109 5510 5859 5943 6097
Size: 782 2904 1366 57 401 349 84 154
Cuts at: 6097 6294 6551 6973 7005 7197 7532 7617
Size: 197 257 422 32 192 335 85
Fragments arranged by size:
2904 1366 782 422 401 349 335 257
197 192 154 85 84 57 32
NaeI GCC'GGC
Cuts at: 0 5518 5979 7617
Size: 5518 461 1638
Fragments arranged by size:
5518 1638 461
NcoI C'CATG_G
Cuts at: 0 2912 7596 7617
Size: 2912 4684 21
Fragments arranged by size:
4684 2912 21
NgoMIV G'CCGG_C
Cuts at: 0 5516 5977 7617
Size: 5516 461 1640
Fragments arranged by size:
5516 1640 461
NheI G'CTAG_C
Cuts at: 0 921 7617
Size: 921 6696
NsiI A_TGCA'T
Cuts at: 0 4413 7617
Size: 4413 3204
NspI r_CATG'y
Cuts at: 0 104 4370 6202 7617
Size: 104 4266 1832 1415
Fragments arranged by size:
4266 1832 1415 104
PasI CC'CwG_GG
Cuts at: 0 634 1179 1902 1972 7617
Size: 634 545 723 70 5645
Fragments arranged by size:
5645 723 634 545 70
PflMI CCAn_nnn'nTGG
Cuts at: 0 1060 2637 4274 5994 6126 7239 7374 7617
Size: 1060 1577 1637 1720 132 1113 135 243
Fragments arranged by size:
1720 1637 1577 1113 1060 243 135 132
PfoI T'CCnGG_A
Cuts at: 0 1244 2828 7609 7617
Size: 1244 1584 4781 8
Fragments arranged by size:
4781 1584 1244 8
PmlI CAC'GTG
Cuts at: 0 402 6066 7617
Size: 402 5664 1551
Fragments arranged by size:
5664 1551 402
PpuMI rG'GwC_Cy
Cuts at: 0 1175 1242 1416 1590 1841 3267 4635 7052
Size: 1175 67 174 174 251 1426 1368 2417
Cuts at: 7052 7617
Size: 565
Fragments arranged by size:
2417 1426 1368 1175 565 251 174 174
67
PshAI GACnn'nnGTC
Cuts at: 0 2900 4930 7617
Size: 2900 2030 2687
Fragments arranged by size:
2900 2687 2030
PspOMI G'GGCC_C
Cuts at: 0 615 1182 4283 7617
Size: 615 567 3101 3334
Fragments arranged by size:
3334 3101 615 567
PspXI vC'TCGA_Gb
Cuts at: 0 358 1003 7617
Size: 358 645 6614
Fragments arranged by size:
6614 645 358
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 6457 6489 7617
Size: 6457 32 1128
Fragments arranged by size:
6457 1128 32
PstI C_TGCA'G
Cuts at: 0 569 742 2168 2748 3478 3492 4047 4556
Size: 569 173 1426 580 730 14 555 509
Cuts at: 4556 5262 5467 5569 5878 6102 6773 6972 7010
Size: 706 205 102 309 224 671 199 38
Cuts at: 7010 7187 7222 7617
Size: 177 35 395
Fragments arranged by size:
1426 730 706 671 580 569 555 509
395 309 224 205 199 177 173 102
38 35 14
PvuII CAG'CTG
Cuts at: 0 782 3686 5052 5109 5859 5943 6097 6294
Size: 782 2904 1366 57 750 84 154 197
Cuts at: 6294 6551 6973 7005 7197 7617
Size: 257 422 32 192 420
Fragments arranged by size:
2904 1366 782 750 422 420 257 197
192 154 84 57 32
SacI G_AGCT'C
Cuts at: 0 521 1202 3514 3574 3823 7617
Size: 521 681 2312 60 249 3794
Fragments arranged by size:
3794 2312 681 521 249 60
SalI G'TCGA_C
Cuts at: 0 3020 7617
Size: 3020 4597
SapI GCTCTTCn'nnn_
Cuts at: 0 1816 3579 6572 7617
Size: 1816 1763 2993 1045
Fragments arranged by size:
2993 1816 1763 1045
SbfI CC_TGCA'GG
Cuts at: 0 742 7617
Size: 742 6875
ScaI AGT'ACT
Cuts at: 0 6139 6709 7617
Size: 6139 570 908
Fragments arranged by size:
6139 908 570
SexAI A'CCwGG_T
Cuts at: 0 7021 7617
Size: 7021 596
SfcI C'TryA_G
Cuts at: 0 115 565 738 869 1604 2164 2725 2744
Size: 115 450 173 131 735 560 561 19
Cuts at: 2744 3474 3488 4043 4435 4531 4552 5258 5463
Size: 730 14 555 392 96 21 706 205
Cuts at: 5463 5565 5601 5874 6098 6507 6769 6968 7006
Size: 102 36 273 224 409 262 199 38
Cuts at: 7006 7183 7218 7617
Size: 177 35 399
Fragments arranged by size:
735 730 706 561 560 555 450 409
399 392 273 262 224 205 199 177
173 131 115 102 96 38 36 35
21 19 14
SmaI CCC'GGG
Cuts at: 0 265 4060 4282 7588 7617
Size: 265 3795 222 3306 29
Fragments arranged by size:
3795 3306 265 222 29
SmlI C'TyrA_G
Cuts at: 0 304 358 526 1003 1387 3922 4614 6306
Size: 304 54 168 477 384 2535 692 1692
Cuts at: 6306 6877 7617
Size: 571 740
Fragments arranged by size:
2535 1692 740 692 571 477 384 304
168 54
SpeI A'CTAG_T
Cuts at: 0 2014 7617
Size: 2014 5603
SphI G_CATG'C
Cuts at: 0 6202 7617
Size: 6202 1415
SspI AAT'ATT
Cuts at: 0 6039 7617
Size: 6039 1578
StuI AGG'CCT
Cuts at: 0 736 4102 7617
Size: 736 3366 3515
Fragments arranged by size:
3515 3366 736
StyI C'CwwG_G
Cuts at: 0 333 418 1412 2189 2912 3874 3911 4259
Size: 333 85 994 777 723 962 37 348
Cuts at: 4259 5885 6689 6884 7596 7617
Size: 1626 804 195 712 21
Fragments arranged by size:
1626 994 962 804 777 723 712 348
333 195 85 37 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 760 1784 2598 4921 7617
Size: 760 1024 814 2323 2696
Fragments arranged by size:
2696 2323 1024 814 760
TatI w'GTAC_w
Cuts at: 0 243 1107 2368 4233 6137 6707 7253 7617
Size: 243 864 1261 1865 1904 570 546 364
Fragments arranged by size:
1904 1865 1261 864 570 546 364 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1537 1569 4611 4643 7617
Size: 1537 32 3042 32 2974
Fragments arranged by size:
3042 2974 1537 32 32
XbaI T'CTAG_A
Cuts at: 0 900 4662 4764 5655 7617
Size: 900 3762 102 891 1962
Fragments arranged by size:
3762 1962 900 891 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 953 5547 5904 7617
Size: 953 4594 357 1713
Fragments arranged by size:
4594 1713 953 357
XhoI C'TCGA_G
Cuts at: 0 358 1003 7617
Size: 358 645 6614
Fragments arranged by size:
6614 645 358
XmaI C'CCGG_G
Cuts at: 0 263 4058 4280 7586 7617
Size: 263 3795 222 3306 31
Fragments arranged by size:
3795 3306 263 222 31
XmnI GAAnn'nnTTC
Cuts at: 0 1382 3191 6566 7617
Size: 1382 1809 3375 1051
Fragments arranged by size:
3375 1809 1382 1051
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI
CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NheI NsiI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AgeI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BsiEI BsiWI BsrGI BssHII BtgZI ClaI
DraI EagI FseI FspI FspAI HpaI KasI MfeI
MluI NarI NdeI NotI NruI PacI PciI PmeI
PpiI PsiI PvuI RsrII SacII SanDI SfiI SfoI
SgfI SgrAI SnaBI SrfI SwaI Tth111I ZraI