(Circular) (Six-base) MAPSORT of: pFN21AA0305.seq Check: 936 from: 1 to: 9322 pFN21AA0305 9322 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7132 278 Size: 53 83 186 936 5596 2468 Fragments arranged by size: 5596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4531 5714 5887 6132 6665 1937 Size: 2594 1183 173 245 533 4594 Fragments arranged by size: 4594 2594 1183 533 245 173 Acc65I G'GTAC_C Cuts at: 6643 6643 Size: 9322 AclI AA'CG_TT Cuts at: 1563 7449 7822 1563 Size: 5886 373 3063 Fragments arranged by size: 5886 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2121 2319 2490 3072 3320 3614 4161 4713 Size: 489 198 171 582 248 294 547 552 Cuts at: 4713 4950 5332 5979 6192 6507 7385 8769 9037 Size: 237 382 647 213 315 878 1384 268 Cuts at: 9037 1632 Size: 1917 Fragments arranged by size: 1917 1384 878 647 582 552 547 489 382 315 294 268 248 237 213 198 171 AflII C'TTAA_G Cuts at: 820 1017 3056 4466 820 Size: 197 2039 1410 5676 Fragments arranged by size: 5676 2039 1410 197 AflIII A'CryG_T Cuts at: 1203 2733 3097 6110 8221 9198 1203 Size: 1530 364 3013 2111 977 1327 Fragments arranged by size: 3013 2111 1530 1327 977 364 AgeI A'CCGG_T Cuts at: 8153 8153 Size: 9322 AhdI GACnn_n'nnGTC Cuts at: 8051 8051 Size: 9322 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9322 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4508 4540 4508 Size: 32 9290 AlwNI CAG_nnn'CTG Cuts at: 3914 4928 5111 6485 7065 8637 3914 Size: 1014 183 1374 580 1572 4599 Fragments arranged by size: 4599 1572 1374 1014 580 183 ApaLI G'TGCA_C Cuts at: 7379 8535 7379 Size: 1156 8166 ApoI r'AATT_y Cuts at: 1493 2680 2956 2971 4280 5219 5529 5815 5901 Size: 1187 276 15 1309 939 310 286 86 Cuts at: 5901 6470 6631 6857 7009 9118 1493 Size: 569 161 226 152 2109 1697 Fragments arranged by size: 2109 1697 1309 1187 939 569 310 286 276 226 161 152 86 15 AseI AT'TA_AT Cuts at: 160 1032 3167 3344 3504 3780 7876 160 Size: 872 2135 177 160 276 4096 1606 Fragments arranged by size: 4096 2135 1606 872 276 177 160 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9322 AvaI C'yCGr_G Cuts at: 1870 1943 6647 1870 Size: 73 4704 4545 Fragments arranged by size: 4704 4545 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4041 4074 4041 Size: 33 9289 BamHI G'GATC_C Cuts at: 6652 7019 6652 Size: 367 8955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6643 8098 618 Size: 325 217 627 4856 1455 1842 Fragments arranged by size: 4856 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 5983 6641 1391 Size: 4592 658 4072 Fragments arranged by size: 4592 4072 658 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3042 3128 3366 3471 4701 5036 928 Size: 971 1143 86 238 105 1230 335 5214 Fragments arranged by size: 5214 1230 1143 971 335 238 105 86 BbvCI CC'TCA_GC Cuts at: 3201 6504 3201 Size: 3303 6019 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4290 4324 7513 7547 1903 Size: 34 2353 34 3189 34 3678 Fragments arranged by size: 3678 3189 2353 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7214 8424 7214 Size: 1210 8112 BclI T'GATC_A Cuts at: 1548 1548 Size: 9322 BfrBI ATG'CAT Cuts at: 2108 2108 Size: 9322 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7933 136 Size: 107 122 71 631 605 6261 1525 Fragments arranged by size: 6261 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2697 9317 2697 Size: 6620 2702 BlpI GC'TnA_GC Cuts at: 6739 6739 Size: 9322 Bme1580I G_kGCm'C Cuts at: 1790 6395 7383 8539 1790 Size: 4605 988 1156 2573 Fragments arranged by size: 4605 2573 1156 988 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7053 8011 442 Size: 416 53 83 399 24 5636 958 1753 Fragments arranged by size: 5636 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 6080 1056 Size: 5024 4298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5112 6036 7982 1239 Size: 3873 924 1946 2579 Fragments arranged by size: 3873 2579 1946 924 Bpu10I CC'TnA_GC Cuts at: 3201 3951 4425 6504 3201 Size: 750 474 2079 6019 Fragments arranged by size: 6019 2079 750 474 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2243 3429 5567 7447 8312 8610 2243 Size: 1186 2138 1880 865 298 2955 Fragments arranged by size: 2955 2138 1880 1186 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6050 7985 882 Size: 617 4551 1935 2219 Fragments arranged by size: 4551 2219 1935 617 BsaAI yAC'GTr Cuts at: 493 1204 6148 7113 493 Size: 711 4944 965 2702 Fragments arranged by size: 4944 2702 965 711 BsaBI GATnn'nnATC Cuts at: 5874 9316 5874 Size: 3442 5880 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7129 7511 8146 275 Size: 53 83 186 936 5596 382 635 1451 Fragments arranged by size: 5596 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7755 8153 8427 8574 8985 7755 Size: 398 274 147 411 8092 Fragments arranged by size: 8092 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 9097 Fragments arranged by size: 9097 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4497 5613 1186 Size: 3311 1116 4895 Fragments arranged by size: 4895 3311 1116 BseYI C'CCAG_C Cuts at: 4037 6159 8525 9021 4037 Size: 2122 2366 496 4338 Fragments arranged by size: 4338 2366 2122 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3012 5619 1163 Size: 573 1276 2607 4866 Fragments arranged by size: 4866 2607 1276 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6772 7533 8561 1540 Size: 275 186 4771 761 1028 2301 Fragments arranged by size: 4771 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5983 6535 7383 7468 8539 5983 Size: 552 848 85 1071 6766 Fragments arranged by size: 6766 1071 848 552 85 BsmI GAATG_Cn' Cuts at: 6833 6926 6833 Size: 93 9229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 5983 6395 6535 6641 7383 7468 8539 Size: 399 4193 412 140 106 742 85 1071 Cuts at: 8539 1391 Size: 2174 Fragments arranged by size: 4193 2174 1071 742 412 399 140 106 85 BspEI T'CCGG_A Cuts at: 8985 8985 Size: 9322 BspHI T'CATG_A Cuts at: 5134 7211 8941 5134 Size: 2077 1730 5515 Fragments arranged by size: 5515 2077 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2921 6677 844 Size: 2077 3756 3489 Fragments arranged by size: 3756 3489 2077 BsrBI CCG'CTC Cuts at: 1394 1823 7209 1394 Size: 429 5386 3507 Fragments arranged by size: 5386 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2165 3526 4306 7817 7991 65 Size: 1183 917 1361 780 3511 174 1396 Fragments arranged by size: 3511 1396 1361 1183 917 780 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7966 8153 1700 Size: 110 6156 187 2869 Fragments arranged by size: 6156 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2098 96 Size: 2002 7320 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9322 BssSI C'ACGA_G Cuts at: 5207 7382 8394 5207 Size: 2175 1012 6135 Fragments arranged by size: 6135 2175 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5618 794 Size: 4824 4498 BstBI TT'CG_AA Cuts at: 5701 8126 5701 Size: 2425 6897 BstEII G'GTnAC_C Cuts at: 1184 3870 4878 1184 Size: 2686 1008 5628 Fragments arranged by size: 5628 2686 1008 BstXI CCAn_nnnn'nTGG Cuts at: 2810 2810 Size: 9322 BstYI r'GATC_y Cuts at: 1972 2697 3373 4148 6482 6652 7019 7404 7421 Size: 725 676 775 2334 170 367 385 17 Cuts at: 7421 8873 8959 8971 9317 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2334 1977 1452 775 725 676 385 367 346 170 86 17 12 BstZ17I GTA'TAC Cuts at: 4532 5715 5888 4532 Size: 1183 173 7966 Fragments arranged by size: 7966 1183 173 Bsu36I CC'TnA_GG Cuts at: 4509 5337 4509 Size: 828 8494 BtgI C'CryG_G Cuts at: 513 1065 2004 5780 6369 6550 513 Size: 552 939 3776 589 181 3285 Fragments arranged by size: 3776 3285 939 589 552 181 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8583 Fragments arranged by size: 8583 683 56 BtsI GCAGTG_nn' Cuts at: 2122 3240 6838 7632 7652 2122 Size: 1118 3598 794 20 3792 Fragments arranged by size: 3792 3598 1118 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5946 5981 529 Size: 35 5382 35 3870 Fragments arranged by size: 5382 3870 35 35 DraI TTT'AAA Cuts at: 2020 2685 2883 6103 6626 2020 Size: 665 198 3220 523 4716 Fragments arranged by size: 4716 3220 665 523 198 DraIII CAC_nnn'GTG Cuts at: 3188 3188 Size: 9322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8329 809 Size: 507 7013 1802 Fragments arranged by size: 7013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5932 6769 7658 Size: 54 1425 50 275 18 4102 837 889 Cuts at: 7658 9287 8 Size: 1629 43 Fragments arranged by size: 4102 1629 1425 889 837 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6769 1537 Size: 275 4957 4090 Fragments arranged by size: 4957 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2883 3091 4601 7252 1353 Size: 1530 208 1510 2651 3423 Fragments arranged by size: 3423 2651 1530 1510 208 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7909 8283 8429 387 Size: 1064 138 6320 374 146 1280 Fragments arranged by size: 6320 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5981 5981 Size: 9322 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2319 2490 3072 3320 3614 4161 Size: 393 489 198 171 582 248 294 547 Cuts at: 4161 4713 4950 5112 5332 5979 6036 6192 6507 Size: 552 237 162 220 647 57 156 315 Cuts at: 6507 7385 7982 8769 9037 1239 Size: 878 597 787 268 1524 Fragments arranged by size: 1524 878 787 647 597 582 552 547 489 393 315 294 268 248 237 220 198 171 162 156 57 EcoO109I rG'GnC_Cy Cuts at: 3994 6558 3994 Size: 2564 6758 EcoRI G'AATT_C Cuts at: 5529 6631 7009 5529 Size: 1102 378 7842 Fragments arranged by size: 7842 1102 378 EcoRV GAT'ATC Cuts at: 6154 6154 Size: 9322 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3470 5362 5532 6512 3470 Size: 1892 170 980 6280 Fragments arranged by size: 6280 1892 980 170 FspI TGC'GCA Cuts at: 7828 7828 Size: 9322 HaeII r_GCGC'y Cuts at: 1678 8469 1678 Size: 6791 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 2689 2721 3104 Size: 32 135 32 881 32 204 32 383 Cuts at: 3104 3136 3662 3694 3883 3915 4432 4464 5343 Size: 32 526 32 189 32 517 32 879 Cuts at: 5343 5375 5483 5515 6200 6232 7963 7995 8037 Size: 32 108 32 685 32 1731 32 42 Cuts at: 8037 8069 1373 Size: 32 2626 Fragments arranged by size: 2626 1731 881 879 685 526 517 383 204 189 135 108 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4963 5695 6666 6912 669 Size: 988 281 3025 732 971 246 3079 Fragments arranged by size: 3079 3025 988 971 732 281 246 HindIII A'AGCT_T Cuts at: 748 5543 748 Size: 4795 4527 HpaI GTT'AAC Cuts at: 4963 6912 4963 Size: 1949 7373 KpnI G_GTAC'C Cuts at: 6647 6647 Size: 9322 MfeI C'AATT_G Cuts at: 2718 3956 6921 2718 Size: 1238 2965 5119 Fragments arranged by size: 5119 2965 1238 MluI A'CGCG_T Cuts at: 9198 9198 Size: 9322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3916 4475 6588 8436 8620 1305 Size: 2611 559 2113 1848 184 2007 Fragments arranged by size: 2611 2113 2007 1848 559 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5934 10 Size: 54 1425 343 4102 3398 Fragments arranged by size: 4102 3398 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 4482 5678 6461 7280 7639 7798 Size: 648 1381 1935 1196 783 819 359 159 Cuts at: 7798 518 Size: 2042 Fragments arranged by size: 2042 1935 1381 1196 819 783 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 4088 6738 7415 8563 8808 1606 Size: 421 2061 2650 677 1148 245 2120 Fragments arranged by size: 2650 2120 2061 1148 677 421 245 NcoI C'CATG_G Cuts at: 513 1065 2004 6369 6550 513 Size: 552 939 4365 181 3285 Fragments arranged by size: 4365 3285 939 552 181 NdeI CA'TA_TG Cuts at: 387 2110 5748 387 Size: 1723 3638 3961 Fragments arranged by size: 3961 3638 1723 NheI G'CTAG_C Cuts at: 1052 6076 1052 Size: 5024 4298 NotI GC'GGCC_GC Cuts at: 6769 6769 Size: 9322 NsiI A_TGCA'T Cuts at: 2110 2110 Size: 9322 NspI r_CATG'y Cuts at: 1132 1229 2737 3101 4320 5834 6114 6680 8225 Size: 97 1508 364 1219 1514 280 566 1545 Cuts at: 8225 1132 Size: 2229 Fragments arranged by size: 2229 1545 1514 1508 1219 566 364 280 97 PasI CC'CwG_GG Cuts at: 1348 5556 1348 Size: 4208 5114 PciI A'CATG_T Cuts at: 2733 3097 6110 8221 2733 Size: 364 3013 2111 3834 Fragments arranged by size: 3834 3013 2111 364 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9322 PmeI GTTT'AAAC Cuts at: 6626 6626 Size: 9322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7391 7423 8904 8936 7391 Size: 32 1481 32 7777 Fragments arranged by size: 7777 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3994 6558 3994 Size: 2564 6758 PshAI GACnn'nnGTC Cuts at: 1613 4701 1613 Size: 3088 6234 PsiI TTA'TAA Cuts at: 3017 6892 3017 Size: 3875 5447 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4672 4704 4672 Size: 32 9290 PstI C_TGCA'G Cuts at: 830 2918 6674 830 Size: 2088 3756 3478 Fragments arranged by size: 3756 3478 2088 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9322 PvuII CAG'CTG Cuts at: 2027 4088 2027 Size: 2061 7261 SacI G_AGCT'C Cuts at: 5983 5983 Size: 9322 SalI G'TCGA_C Cuts at: 1936 6664 1936 Size: 4728 4594 SbfI CC_TGCA'GG Cuts at: 6674 6674 Size: 9322 ScaI AGT'ACT Cuts at: 5811 7570 9282 5811 Size: 1759 1712 5851 Fragments arranged by size: 5851 1759 1712 SexAI A'CCwGG_T Cuts at: 4573 4573 Size: 9322 SfcI C'TryA_G Cuts at: 826 1045 2914 3429 4074 4083 4761 5112 6670 Size: 219 1869 515 645 9 678 351 1558 Cuts at: 6670 7805 8486 8677 826 Size: 1135 681 191 1471 Fragments arranged by size: 1869 1558 1471 1135 681 678 645 515 351 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9322 SmaI CCC'GGG Cuts at: 1872 6649 1872 Size: 4777 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 3056 3444 4466 5546 7426 Size: 197 926 315 798 388 1022 1080 1880 Cuts at: 7426 8327 8589 820 Size: 901 262 1553 Fragments arranged by size: 1880 1553 1080 1022 926 901 798 388 315 262 197 SnaBI TAC'GTA Cuts at: 493 7113 493 Size: 6620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9322 SphI G_CATG'C Cuts at: 1132 6680 1132 Size: 5548 3774 SspI AAT'ATT Cuts at: 5 52 3556 5246 5413 7246 5 Size: 47 3504 1690 167 1833 2081 Fragments arranged by size: 3504 2081 1833 1690 167 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9322 StyI C'CwwG_G Cuts at: 513 1065 2004 6369 6550 6761 513 Size: 552 939 4365 181 211 3074 Fragments arranged by size: 4365 3074 939 552 211 181 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5585 7349 7366 7519 7704 8149 325 Size: 1255 4005 1764 17 153 185 445 1498 Fragments arranged by size: 4005 1764 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2098 4490 5790 Size: 274 80 33 51 1443 121 2392 1300 Cuts at: 5790 5809 5858 7568 9280 96 Size: 19 49 1710 1712 138 Fragments arranged by size: 2392 1712 1710 1443 1300 274 138 121 80 51 49 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5920 5952 9282 9314 5920 Size: 32 3330 32 5928 Fragments arranged by size: 5928 3330 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9322 XbaI T'CTAG_A Cuts at: 4372 6658 4372 Size: 2286 7036 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9322 XmaI C'CCGG_G Cuts at: 1870 6647 1870 Size: 4777 4545 XmnI GAAnn'nnTTC Cuts at: 5169 7451 5169 Size: 2282 7040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7130 276 Size: 53 83 186 936 5596 2468 Fragments arranged by size: 5596 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BplI BsiWI BsmBI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI