(Circular) (Six-base) MAPSORT of: pFN21AA0109.seq Check: 7625 from: 1 to: 6010 pFN21AA0109 6010 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2119 2563 2995 2119 Size: 444 432 5134 Fragments arranged by size: 5134 444 432 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3820 278 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2061 3353 1937 Size: 124 1292 4594 Fragments arranged by size: 4594 1292 124 Acc65I G'GTAC_C Cuts at: 3331 3331 Size: 6010 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4073 5457 5725 1632 Size: 2441 1384 268 1917 Fragments arranged by size: 2441 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 4909 5886 1203 Size: 3706 977 1327 Fragments arranged by size: 3706 1327 977 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 2754 1166 Size: 1588 4422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2742 1184 Size: 1558 4452 AlwNI CAG_nnn'CTG Cuts at: 2578 3753 5325 2578 Size: 1175 1572 3263 Fragments arranged by size: 3263 1572 1175 ApaLI G'TGCA_C Cuts at: 4067 5223 4067 Size: 1156 4854 ApoI r'AATT_y Cuts at: 1493 2243 2371 3319 3545 3697 5806 1493 Size: 750 128 948 226 152 2109 1697 Fragments arranged by size: 2109 1697 948 750 226 152 128 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 2055 3335 1870 Size: 73 112 1280 4545 Fragments arranged by size: 4545 1280 112 73 BamHI G'GATC_C Cuts at: 2973 3340 3707 2973 Size: 367 367 5276 Fragments arranged by size: 5276 367 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3178 3331 4786 618 Size: 325 217 627 1391 153 1455 1842 Fragments arranged by size: 1842 1455 1391 627 325 217 153 BanII G_rGCy'C Cuts at: 1391 2523 3151 3329 1391 Size: 1132 628 178 4072 Fragments arranged by size: 4072 1132 628 178 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2230 928 Size: 971 331 4708 Fragments arranged by size: 4708 971 331 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4201 4235 1903 Size: 34 2264 34 3678 Fragments arranged by size: 3678 2264 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3902 5112 3902 Size: 1210 4800 BclI T'GATC_A Cuts at: 1548 1548 Size: 6010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3288 4621 136 Size: 107 122 71 631 605 1616 1333 1525 Fragments arranged by size: 1616 1525 1333 631 605 122 107 71 BglII A'GATC_T Cuts at: 6005 6005 Size: 6010 BlpI GC'TnA_GC Cuts at: 3427 3427 Size: 6010 Bme1580I G_kGCm'C Cuts at: 1790 2587 4071 5227 1790 Size: 797 1484 1156 2573 Fragments arranged by size: 2573 1484 1156 797 BmgBI CAC'GTC Cuts at: 1318 2266 1318 Size: 948 5062 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2481 3741 4699 Size: 416 53 83 399 24 1064 1260 958 Cuts at: 4699 442 Size: 1753 Fragments arranged by size: 1753 1260 1064 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2558 2927 4670 1239 Size: 1319 369 1743 2579 Fragments arranged by size: 2579 1743 1319 369 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3185 4135 5000 5298 3185 Size: 950 865 298 3897 Fragments arranged by size: 3897 950 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4673 882 Size: 617 3174 2219 Fragments arranged by size: 3174 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3801 493 Size: 711 2597 2702 Fragments arranged by size: 2702 2597 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3817 4199 4834 275 Size: 53 83 186 936 2284 382 635 1451 Fragments arranged by size: 2284 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4443 4841 5115 5262 5673 4443 Size: 398 274 147 411 4780 Fragments arranged by size: 4780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5785 Fragments arranged by size: 5785 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6010 BseYI C'CCAG_C Cuts at: 5213 5709 5213 Size: 496 5514 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2725 3023 1163 Size: 573 989 298 4150 Fragments arranged by size: 4150 989 573 298 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3460 4221 5249 1540 Size: 275 186 1459 761 1028 2301 Fragments arranged by size: 2301 1459 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2049 2325 2523 4071 4156 5227 2049 Size: 276 198 1548 85 1071 2832 Fragments arranged by size: 2832 1548 1071 276 198 85 BsmI GAATG_Cn' Cuts at: 2642 3055 3521 3614 2642 Size: 413 466 93 5038 Fragments arranged by size: 5038 466 413 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2325 2523 2587 3151 3329 4071 Size: 399 259 276 198 64 564 178 742 Cuts at: 4071 4156 5227 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 564 399 276 259 198 178 85 64 BspEI T'CCGG_A Cuts at: 5673 5673 Size: 6010 BspHI T'CATG_A Cuts at: 3899 5629 3899 Size: 1730 4280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 2559 2563 2995 3365 844 Size: 1275 440 4 432 370 3489 Fragments arranged by size: 3489 1275 440 432 370 4 BsrBI CCG'CTC Cuts at: 1394 1823 2712 3897 1394 Size: 429 889 1185 3507 Fragments arranged by size: 3507 1185 889 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2151 4505 4679 65 Size: 1183 903 2354 174 1396 Fragments arranged by size: 2354 1396 1183 903 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4654 4841 1700 Size: 110 2844 187 2869 Fragments arranged by size: 2869 2844 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 2385 1925 Size: 460 5550 BssSI C'ACGA_G Cuts at: 4070 5082 4070 Size: 1012 4998 BstAPI GCAn_nnn'nTGC Cuts at: 794 2093 2131 794 Size: 1299 38 4673 Fragments arranged by size: 4673 1299 38 BstBI TT'CG_AA Cuts at: 2241 4814 2241 Size: 2573 3437 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstXI CCAn_nnnn'nTGG Cuts at: 2471 2471 Size: 6010 BstYI r'GATC_y Cuts at: 1972 2973 3340 3707 4092 4109 5561 5647 5659 Size: 1001 367 367 385 17 1452 86 12 Cuts at: 5659 6005 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1001 385 367 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2232 513 Size: 552 1167 4291 Fragments arranged by size: 4291 1167 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 2094 2945 3292 3526 4320 4340 2094 Size: 851 347 234 794 20 3764 Fragments arranged by size: 3764 851 794 347 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 2938 3314 2938 Size: 376 5634 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3254 5017 809 Size: 507 1938 1763 1802 Fragments arranged by size: 1938 1802 1763 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3280 3457 4346 Size: 54 1425 50 275 18 1450 177 889 Cuts at: 4346 5975 8 Size: 1629 43 Fragments arranged by size: 1629 1450 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3457 1537 Size: 275 1645 4090 Fragments arranged by size: 4090 1645 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2436 2528 2606 3940 1353 Size: 1083 92 78 1334 3423 Fragments arranged by size: 3423 1334 1083 92 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4597 4971 5117 387 Size: 1064 138 3008 374 146 1280 Fragments arranged by size: 3008 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2521 2521 Size: 6010 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2558 2927 4073 4670 5457 5725 1239 Size: 393 926 369 1146 597 787 268 1524 Fragments arranged by size: 1524 1146 926 787 597 393 369 268 EcoRI G'AATT_C Cuts at: 2243 2371 3319 3697 2243 Size: 128 948 378 4556 Fragments arranged by size: 4556 948 378 128 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2950 2950 Size: 6010 FspI TGC'GCA Cuts at: 2135 4516 2135 Size: 2381 3629 HaeII r_GCGC'y Cuts at: 1678 5157 1678 Size: 3479 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2413 2445 2484 Size: 32 135 32 486 32 323 32 39 Cuts at: 2484 2516 4651 4683 4725 4757 1373 Size: 32 2135 32 42 32 2626 Fragments arranged by size: 2626 2135 486 323 135 42 39 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2224 3354 3600 669 Size: 988 281 286 1130 246 3079 Fragments arranged by size: 3079 1130 988 286 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6010 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KpnI G_GTAC'C Cuts at: 3335 3335 Size: 6010 MfeI C'AATT_G Cuts at: 2724 3609 2724 Size: 885 5125 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 2492 2955 5124 5308 1305 Size: 906 281 463 2169 184 2007 Fragments arranged by size: 2169 2007 906 463 281 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4188 Fragments arranged by size: 4188 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2754 3164 3968 4327 4486 518 Size: 648 1588 410 804 359 159 2042 Fragments arranged by size: 2042 1588 804 648 410 359 159 MspA1I CmG'CkG Cuts at: 1606 2693 3426 4103 5251 5496 1606 Size: 1087 733 677 1148 245 2120 Fragments arranged by size: 2120 1148 1087 733 677 245 NcoI C'CATG_G Cuts at: 513 1065 2232 513 Size: 552 1167 4291 Fragments arranged by size: 4291 1167 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 2632 3368 4913 1132 Size: 97 1403 736 1545 2229 Fragments arranged by size: 2229 1545 1403 736 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6010 PciI A'CATG_T Cuts at: 4909 4909 Size: 6010 PflMI CCAn_nnn'nTGG Cuts at: 1108 2193 3060 1108 Size: 1085 867 4058 Fragments arranged by size: 4058 1085 867 PfoI T'CCnGG_A Cuts at: 2526 2526 Size: 6010 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PstI C_TGCA'G Cuts at: 830 3362 830 Size: 2532 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2693 2693 Size: 6010 SacI G_AGCT'C Cuts at: 2523 2523 Size: 6010 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SapI GCTCTTCn'nnn_ Cuts at: 2436 2528 2606 2436 Size: 92 78 5840 Fragments arranged by size: 5840 92 78 SbfI CC_TGCA'GG Cuts at: 3362 3362 Size: 6010 ScaI AGT'ACT Cuts at: 4258 5970 4258 Size: 1712 4298 SfcI C'TryA_G Cuts at: 826 1045 3241 3358 4493 5174 5365 826 Size: 219 2196 117 1135 681 191 1471 Fragments arranged by size: 2196 1471 1135 681 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 3337 1872 Size: 1465 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3200 4114 5015 5277 820 Size: 197 926 1257 914 901 262 1553 Fragments arranged by size: 1553 1257 926 914 901 262 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 2632 3368 1132 Size: 1500 736 3774 Fragments arranged by size: 3774 1500 736 SspI AAT'ATT Cuts at: 5 52 3934 5 Size: 47 3882 2081 Fragments arranged by size: 3882 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6010 StyI C'CwwG_G Cuts at: 513 1065 2232 2844 3449 513 Size: 552 1167 612 605 3074 Fragments arranged by size: 3074 1167 612 605 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3256 4037 4054 4207 4392 4837 325 Size: 1255 1676 781 17 153 185 445 1498 Fragments arranged by size: 1676 1498 1255 781 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3033 4256 5968 Size: 274 80 33 51 1443 1056 1223 1712 Cuts at: 5968 96 Size: 138 Fragments arranged by size: 1712 1443 1223 1056 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5970 6002 5970 Size: 32 5978 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6010 XbaI T'CTAG_A Cuts at: 2350 3346 2350 Size: 996 5014 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2194 2194 Size: 6010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 3335 1870 Size: 1465 4545 XmnI GAAnn'nnTTC Cuts at: 2396 4139 2396 Size: 1743 4267 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3818 276 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI