(Circular) (Six-base) MAPSORT of: pFN21AA0103.seq Check: 4574 from: 1 to: 5596 pFN21AA0103 5596 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2603 2939 1937 Size: 666 336 4594 Fragments arranged by size: 4594 666 336 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2613 3659 5043 5311 1632 Size: 981 1046 1384 268 1917 Fragments arranged by size: 1917 1384 1046 981 268 AflII C'TTAA_G Cuts at: 820 1017 2849 820 Size: 197 1832 3567 Fragments arranged by size: 3567 1832 197 AflIII A'CryG_T Cuts at: 1203 2865 4495 5472 1203 Size: 1662 1630 977 1327 Fragments arranged by size: 1662 1630 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2502 1184 Size: 1318 4278 AlwNI CAG_nnn'CTG Cuts at: 3339 4911 3339 Size: 1572 4024 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 2123 2127 4150 160 Size: 872 1091 4 2023 1606 Fragments arranged by size: 2023 1606 1091 872 4 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2921 1870 Size: 73 978 4545 Fragments arranged by size: 4545 978 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2917 4372 618 Size: 325 217 627 1130 1455 1842 Fragments arranged by size: 1842 1455 1130 627 325 217 BanII G_rGCy'C Cuts at: 1391 2915 1391 Size: 1524 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2222 2251 2868 928 Size: 971 323 29 617 3656 Fragments arranged by size: 3656 971 617 323 29 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4207 136 Size: 107 122 71 631 605 2535 1525 Fragments arranged by size: 2535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 3657 4813 1790 Size: 1867 1156 2573 Fragments arranged by size: 2573 1867 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2884 3327 4285 Size: 416 53 83 399 24 1467 443 958 Cuts at: 4285 442 Size: 1753 Fragments arranged by size: 1753 1467 958 443 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4256 1239 Size: 3017 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2068 3721 4586 4884 2068 Size: 1653 865 298 2780 Fragments arranged by size: 2780 1653 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2020 2457 4259 882 Size: 617 521 437 1802 2219 Fragments arranged by size: 2219 1802 617 521 437 BsaAI yAC'GTr Cuts at: 493 1204 3387 493 Size: 711 2183 2702 Fragments arranged by size: 2702 2183 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3403 3785 4420 275 Size: 53 83 186 936 1870 382 635 1451 Fragments arranged by size: 1870 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5371 Fragments arranged by size: 5371 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5596 BseYI C'CCAG_C Cuts at: 2146 4799 5295 2146 Size: 2653 496 2447 Fragments arranged by size: 2653 2447 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5023 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3657 3742 4813 3657 Size: 85 1071 4440 Fragments arranged by size: 4440 1071 85 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2915 3657 3742 4813 1391 Size: 399 1125 742 85 1071 2174 Fragments arranged by size: 2174 1125 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2807 2951 844 Size: 1963 144 3489 Fragments arranged by size: 3489 1963 144 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2876 4091 4265 65 Size: 1183 1628 1215 174 1396 Fragments arranged by size: 1628 1396 1215 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5596 BstBI TT'CG_AA Cuts at: 2395 4400 2395 Size: 2005 3591 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2353 2926 3293 3678 3695 5147 5233 5245 Size: 381 573 367 385 17 1452 86 12 Cuts at: 5245 5591 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 573 385 381 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2604 2604 Size: 5596 BtgI C'CryG_G Cuts at: 513 1065 2004 2301 513 Size: 552 939 297 3808 Fragments arranged by size: 3808 939 552 297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 2364 3112 3906 3926 2364 Size: 748 794 20 4034 Fragments arranged by size: 4034 794 748 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3043 3932 5561 Size: 54 1425 50 275 18 1213 889 1629 Cuts at: 5561 8 Size: 43 Fragments arranged by size: 1629 1425 1213 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3526 1353 Size: 2173 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2613 3659 4256 5043 5311 1239 Size: 393 981 1046 597 787 268 1524 Fragments arranged by size: 1524 1046 981 787 597 393 268 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2633 2633 Size: 5596 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 4743 1678 Size: 3065 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 4237 4269 4311 Size: 32 135 32 970 32 1663 32 42 Cuts at: 4311 4343 1373 Size: 32 2626 Fragments arranged by size: 2626 1663 970 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2092 2381 2440 2850 4710 4894 1305 Size: 787 289 59 410 1860 184 2007 Fragments arranged by size: 2007 1860 787 410 289 184 59 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3774 Fragments arranged by size: 3774 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2514 3554 3913 4072 518 Size: 648 1348 1040 359 159 2042 Fragments arranged by size: 2042 1348 1040 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3012 3689 4837 5082 1606 Size: 1406 677 1148 245 2120 Fragments arranged by size: 2120 1406 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2301 513 Size: 552 939 297 3808 Fragments arranged by size: 3808 939 552 297 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2869 2954 4499 1132 Size: 97 1640 85 1545 2229 Fragments arranged by size: 2229 1640 1545 97 85 PacI TTA_AT'TAA Cuts at: 2127 2127 Size: 5596 PasI CC'CwG_GG Cuts at: 1348 2404 1348 Size: 1056 4540 PciI A'CATG_T Cuts at: 2865 4495 2865 Size: 1630 3966 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2202 1613 Size: 589 5007 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PstI C_TGCA'G Cuts at: 830 2948 830 Size: 2118 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2944 4079 4760 4951 826 Size: 219 1899 1135 681 191 1471 Fragments arranged by size: 1899 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2923 1872 Size: 1051 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2083 2849 3700 4601 4863 820 Size: 197 926 140 766 851 901 262 1553 Fragments arranged by size: 1553 926 901 851 766 262 197 140 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 2843 3520 5 Size: 47 2791 677 2081 Fragments arranged by size: 2791 2081 677 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2004 2301 2608 3035 513 Size: 552 939 297 307 427 3074 Fragments arranged by size: 3074 939 552 427 307 297 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2903 3623 3640 3793 3978 4423 325 Size: 1255 1323 720 17 153 185 445 1498 Fragments arranged by size: 1498 1323 1255 720 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5556 5588 5556 Size: 32 5564 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2921 1870 Size: 1051 4545 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI