(Circular) (Six-base) MAPSORT of: pFN21AA0095.seq Check: 8693 from: 1 to: 7162 pFN21AA0095 7162 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2613 2613 Size: 7162 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3201 4972 278 Size: 53 83 186 936 1665 1771 2468 Fragments arranged by size: 2468 1771 1665 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4505 1937 Size: 2568 4594 Acc65I G'GTAC_C Cuts at: 4483 4483 Size: 7162 AclI AA'CG_TT Cuts at: 1563 5289 5662 1563 Size: 3726 373 3063 Fragments arranged by size: 3726 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2018 2330 2750 3272 3521 4013 5225 6609 Size: 386 312 420 522 249 492 1212 1384 Cuts at: 6609 6877 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1212 522 492 420 386 312 268 249 AfeI AGC'GCT Cuts at: 4151 4151 Size: 7162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6965 AflIII A'CryG_T Cuts at: 1203 2670 2700 3696 6061 7038 1203 Size: 1467 30 996 2365 977 1327 Fragments arranged by size: 2365 1467 1327 996 977 30 AgeI A'CCGG_T Cuts at: 5993 5993 Size: 7162 AhdI GACnn_n'nnGTC Cuts at: 5891 5891 Size: 7162 AleI CACnn'nnGTG Cuts at: 1166 2816 1166 Size: 1650 5512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7162 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4052 4084 4052 Size: 32 7130 AlwNI CAG_nnn'CTG Cuts at: 4905 6477 4905 Size: 1572 5590 ApaI G_GGCC'C Cuts at: 3139 3139 Size: 7162 ApaLI G'TGCA_C Cuts at: 5219 6375 5219 Size: 1156 6006 ApoI r'AATT_y Cuts at: 1493 2433 2568 2833 4052 4210 4471 4697 4849 Size: 940 135 265 1219 158 261 226 152 Cuts at: 4849 6958 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1219 940 265 261 226 158 152 135 AseI AT'TA_AT Cuts at: 160 1032 5716 160 Size: 872 4684 1606 Fragments arranged by size: 4684 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 AvaI C'yCGr_G Cuts at: 1870 1943 2195 2202 4487 1870 Size: 73 252 7 2285 4545 Fragments arranged by size: 4545 2285 252 73 7 BamHI G'GATC_C Cuts at: 4492 4859 4492 Size: 367 6795 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2860 3284 4162 4483 5938 Size: 325 217 627 1073 424 878 321 1455 Cuts at: 5938 618 Size: 1842 Fragments arranged by size: 1842 1455 1073 878 627 424 325 321 217 BanII G_rGCy'C Cuts at: 1391 2031 3028 3139 3947 4033 4481 1391 Size: 640 997 111 808 86 448 4072 Fragments arranged by size: 4072 997 808 640 448 111 86 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2469 3348 928 Size: 971 570 879 4742 Fragments arranged by size: 4742 971 879 570 BbvCI CC'TCA_GC Cuts at: 2379 3542 2379 Size: 1163 5999 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4420 4454 5353 5387 1903 Size: 34 2483 34 899 34 3678 Fragments arranged by size: 3678 2483 899 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 5054 6264 2002 Size: 3052 1210 2900 Fragments arranged by size: 3052 2900 1210 BclI T'GATC_A Cuts at: 1548 4089 1548 Size: 2541 4621 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5773 136 Size: 107 122 71 631 605 4101 1525 Fragments arranged by size: 4101 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7157 7157 Size: 7162 BlpI GC'TnA_GC Cuts at: 3534 3941 4579 3534 Size: 407 638 6117 Fragments arranged by size: 6117 638 407 Bme1580I G_kGCm'C Cuts at: 1790 3139 3970 4167 5223 6379 1790 Size: 1349 831 197 1056 1156 2573 Fragments arranged by size: 2573 1349 1156 1056 831 197 BmgBI CAC'GTC Cuts at: 1318 2157 2701 1318 Size: 839 544 5779 Fragments arranged by size: 5779 839 544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4893 5851 442 Size: 416 53 83 399 24 3476 958 1753 Fragments arranged by size: 3476 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7162 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2089 2089 Size: 7162 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3632 3810 3941 5822 1239 Size: 2393 178 131 1881 2579 Fragments arranged by size: 2579 2393 1881 178 131 Bpu10I CC'TnA_GC Cuts at: 2379 3542 2379 Size: 1163 5999 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2294 2466 2810 4017 4277 5287 6152 6450 2294 Size: 172 344 1207 260 1010 865 298 3006 Fragments arranged by size: 3006 1207 1010 865 344 298 260 172 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2205 2985 5825 882 Size: 617 706 780 2840 2219 Fragments arranged by size: 2840 2219 780 706 617 BsaAI yAC'GTr Cuts at: 493 1204 4953 493 Size: 711 3749 2702 Fragments arranged by size: 3749 2702 711 BsaBI GATnn'nnATC Cuts at: 7156 7156 Size: 7162 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2183 2466 3198 4969 Size: 53 83 186 936 650 283 732 1771 Cuts at: 4969 5351 5986 275 Size: 382 635 1451 Fragments arranged by size: 1771 1451 936 732 650 635 382 283 186 83 53 BsaWI w'CCGG_w Cuts at: 5595 5993 6267 6414 6825 5595 Size: 398 274 147 411 5932 Fragments arranged by size: 5932 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2387 2417 1178 Size: 30 165 30 984 30 5923 Fragments arranged by size: 5923 984 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2396 3530 3587 1186 Size: 997 213 1134 57 4761 Fragments arranged by size: 4761 1134 997 213 57 BseYI C'CCAG_C Cuts at: 2229 2847 2851 3027 3037 3097 4326 6365 6861 Size: 618 4 176 10 60 1229 2039 496 Cuts at: 6861 2229 Size: 2530 Fragments arranged by size: 2530 2039 1229 618 496 176 60 10 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2271 2423 4460 1163 Size: 573 535 152 2037 3865 Fragments arranged by size: 3865 2037 573 535 152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4612 5373 6401 1540 Size: 275 186 2611 761 1028 2301 Fragments arranged by size: 2611 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 3535 5223 5308 6379 2031 Size: 1504 1688 85 1071 2814 Fragments arranged by size: 2814 1688 1504 1071 85 BsmI GAATG_Cn' Cuts at: 3910 4673 4766 3910 Size: 763 93 6306 Fragments arranged by size: 6306 763 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2159 2159 Size: 7162 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 3028 3139 3535 3947 3970 4033 Size: 399 241 997 111 396 412 23 63 Cuts at: 4033 4167 4481 5223 5308 6379 1391 Size: 134 314 742 85 1071 2174 Fragments arranged by size: 2174 1071 997 742 412 399 396 314 241 134 111 85 63 23 BspEI T'CCGG_A Cuts at: 6825 6825 Size: 7162 BspHI T'CATG_A Cuts at: 4455 5051 6781 4455 Size: 596 1730 4836 Fragments arranged by size: 4836 1730 596 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2613 3003 4517 844 Size: 1769 390 1514 3489 Fragments arranged by size: 3489 1769 1514 390 BsrBI CCG'CTC Cuts at: 1394 1823 3454 5049 1394 Size: 429 1631 1595 3507 Fragments arranged by size: 3507 1631 1595 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4011 5657 5831 65 Size: 1183 2763 1646 174 1396 Fragments arranged by size: 2763 1646 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2713 5806 5993 1700 Size: 110 903 3093 187 2869 Fragments arranged by size: 3093 2869 903 187 110 BsrGI T'GTAC_A Cuts at: 96 3606 96 Size: 3510 3652 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7162 BssSI C'ACGA_G Cuts at: 3548 5222 6234 3548 Size: 1674 1012 4476 Fragments arranged by size: 4476 1674 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2446 2723 3431 794 Size: 1652 277 708 4525 Fragments arranged by size: 4525 1652 708 277 BstBI TT'CG_AA Cuts at: 2884 5966 2884 Size: 3082 4080 BstEII G'GTnAC_C Cuts at: 1184 3557 1184 Size: 2373 4789 BstXI CCAn_nnnn'nTGG Cuts at: 3554 3554 Size: 7162 BstYI r'GATC_y Cuts at: 1972 2109 3154 4492 4859 5244 5261 6713 6799 Size: 137 1045 1338 367 385 17 1452 86 Cuts at: 6799 6811 7157 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1338 1045 385 367 346 137 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3140 3665 3140 Size: 525 6637 BtgI C'CryG_G Cuts at: 513 1065 2004 2679 3627 513 Size: 552 939 675 948 4048 Fragments arranged by size: 4048 948 939 675 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6423 Fragments arranged by size: 6423 683 56 BtsI GCAGTG_nn' Cuts at: 2444 4678 5472 5492 2444 Size: 2234 794 20 4114 Fragments arranged by size: 4114 2234 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7162 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2245 2280 529 Size: 35 1681 35 5411 Fragments arranged by size: 5411 1681 35 35 DraI TTT'AAA Cuts at: 3049 3513 4466 3049 Size: 464 953 5745 Fragments arranged by size: 5745 953 464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2523 6169 809 Size: 507 1207 3646 1802 Fragments arranged by size: 3646 1802 1207 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4609 5498 7127 Size: 54 1425 50 275 18 2779 889 1629 Cuts at: 7127 8 Size: 43 Fragments arranged by size: 2779 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4609 1537 Size: 275 2797 4090 Fragments arranged by size: 4090 2797 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2343 2354 3867 4182 5092 1353 Size: 990 11 1513 315 910 3423 Fragments arranged by size: 3423 1513 990 910 315 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3239 5749 6123 6269 387 Size: 1064 138 1650 2510 374 146 1280 Fragments arranged by size: 2510 1650 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2029 2029 Size: 7162 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2018 2330 2750 3272 3521 3632 3810 Size: 393 386 312 420 522 249 111 178 Cuts at: 3810 3941 4013 5225 5822 6609 6877 1239 Size: 131 72 1212 597 787 268 1524 Fragments arranged by size: 1524 1212 787 597 522 420 393 386 312 268 249 178 131 111 72 EcoNI CCTnn'n_nnAGG Cuts at: 4172 4172 Size: 7162 EcoO109I rG'GnC_Cy Cuts at: 2364 2516 3135 3136 3643 4323 4450 2364 Size: 152 619 1 507 680 127 5076 Fragments arranged by size: 5076 680 619 507 152 127 1 EcoRI G'AATT_C Cuts at: 2433 4471 4849 2433 Size: 2038 378 4746 Fragments arranged by size: 4746 2038 378 EcoRV GAT'ATC Cuts at: 4141 4141 Size: 7162 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2383 2486 4268 2383 Size: 103 1782 5277 Fragments arranged by size: 5277 1782 103 FspI TGC'GCA Cuts at: 2756 5668 2756 Size: 2912 4250 FspAI rTGC'GCAy Cuts at: 2756 2756 Size: 7162 HaeII r_GCGC'y Cuts at: 1678 2131 2966 4153 6309 1678 Size: 453 835 1187 2156 2531 Fragments arranged by size: 2531 2156 1187 835 453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3183 3215 4334 4366 5803 Size: 32 135 32 1611 32 1119 32 1437 Cuts at: 5803 5835 5877 5909 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1611 1437 1119 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2709 4506 4752 669 Size: 988 281 771 1797 246 3079 Fragments arranged by size: 3079 1797 988 771 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7162 HpaI GTT'AAC Cuts at: 4752 4752 Size: 7162 KpnI G_GTAC'C Cuts at: 4487 4487 Size: 7162 MfeI C'AATT_G Cuts at: 2876 4761 2876 Size: 1885 5277 MluI A'CGCG_T Cuts at: 7038 7038 Size: 7162 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2691 3318 4002 6276 6460 1305 Size: 1386 627 684 2274 184 2007 Fragments arranged by size: 2274 2007 1386 684 627 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5340 Fragments arranged by size: 5340 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2549 2816 5120 5479 5638 518 Size: 648 1383 267 2304 359 159 2042 Fragments arranged by size: 2304 2042 1383 648 359 267 159 MspA1I CmG'CkG Cuts at: 1606 2851 3037 4578 5255 6403 6648 1606 Size: 1245 186 1541 677 1148 245 2120 Fragments arranged by size: 2120 1541 1245 1148 677 245 186 NcoI C'CATG_G Cuts at: 513 1065 2004 2679 513 Size: 552 939 675 4996 Fragments arranged by size: 4996 939 675 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7162 NotI GC'GGCC_GC Cuts at: 4609 4609 Size: 7162 NspI r_CATG'y Cuts at: 1132 1229 2674 2983 3700 4520 6065 1132 Size: 97 1445 309 717 820 1545 2229 Fragments arranged by size: 2229 1545 1445 820 717 309 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7162 PciI A'CATG_T Cuts at: 2670 3696 6061 2670 Size: 1026 2365 3771 Fragments arranged by size: 3771 2365 1026 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 1108 Size: 1128 6034 PfoI T'CCnGG_A Cuts at: 2354 3060 2354 Size: 706 6456 PmeI GTTT'AAAC Cuts at: 4466 4466 Size: 7162 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5231 5263 6744 6776 5231 Size: 32 1481 32 5617 Fragments arranged by size: 5617 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2364 2516 4450 2364 Size: 152 1934 5076 Fragments arranged by size: 5076 1934 152 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7162 PsiI TTA'TAA Cuts at: 2802 4732 2802 Size: 1930 5232 PspOMI G'GGCC_C Cuts at: 3135 3135 Size: 7162 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2085 2117 2085 Size: 32 7130 PstI C_TGCA'G Cuts at: 830 2610 4514 830 Size: 1780 1904 3478 Fragments arranged by size: 3478 1904 1780 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7162 PvuII CAG'CTG Cuts at: 2851 3037 2851 Size: 186 6976 SacI G_AGCT'C Cuts at: 2031 2031 Size: 7162 SalI G'TCGA_C Cuts at: 1936 4504 1936 Size: 2568 4594 SanDI GG'GwC_CC Cuts at: 2516 2516 Size: 7162 SbfI CC_TGCA'GG Cuts at: 4514 4514 Size: 7162 ScaI AGT'ACT Cuts at: 3653 5410 7122 3653 Size: 1757 1712 3693 Fragments arranged by size: 3693 1757 1712 SexAI A'CCwGG_T Cuts at: 2616 3053 2616 Size: 437 6725 SfcI C'TryA_G Cuts at: 826 1045 2606 2927 4510 5645 6326 6517 826 Size: 219 1561 321 1583 1135 681 191 1471 Fragments arranged by size: 1583 1561 1471 1135 681 321 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7162 SmaI CCC'GGG Cuts at: 1872 4489 1872 Size: 2617 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2273 2481 2789 4032 4292 5266 Size: 197 926 330 208 308 1243 260 974 Cuts at: 5266 6167 6429 820 Size: 901 262 1553 Fragments arranged by size: 1553 1243 974 926 901 330 308 262 260 208 197 SnaBI TAC'GTA Cuts at: 493 4953 493 Size: 4460 2702 SpeI A'CTAG_T Cuts at: 152 3809 152 Size: 3657 3505 SphI G_CATG'C Cuts at: 1132 2983 4520 1132 Size: 1851 1537 3774 Fragments arranged by size: 3774 1851 1537 SspI AAT'ATT Cuts at: 5 52 5086 5 Size: 47 5034 2081 Fragments arranged by size: 5034 2081 47 StuI AGG'CCT Cuts at: 1517 2777 1517 Size: 1260 5902 StyI C'CwwG_G Cuts at: 513 1065 2004 2679 2778 3635 4601 513 Size: 552 939 675 99 857 966 3074 Fragments arranged by size: 3074 966 939 857 675 552 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5189 5206 5359 5544 5989 325 Size: 1255 3609 17 153 185 445 1498 Fragments arranged by size: 3609 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3066 3174 3606 Size: 274 80 33 51 1443 1089 108 432 Cuts at: 3606 3651 5408 7120 96 Size: 45 1757 1712 138 Fragments arranged by size: 1757 1712 1443 1089 432 274 138 108 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3230 3262 7122 7154 3230 Size: 32 3860 32 3238 Fragments arranged by size: 3860 3238 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7162 XbaI T'CTAG_A Cuts at: 4498 4498 Size: 7162 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4432 4432 Size: 7162 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7162 XmaI C'CCGG_G Cuts at: 1870 4487 1870 Size: 2617 4545 XmnI GAAnn'nnTTC Cuts at: 4248 5291 4248 Size: 1043 6119 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3199 4970 276 Size: 53 83 186 936 1665 1771 2468 Fragments arranged by size: 2468 1771 1665 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SapI SfiI SfoI SrfI SwaI