(Circular) (Six-base) MAPSORT of: pFN21AA0094.seq Check: 5682 from: 1 to: 5863 pFN21AA0094 5863 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3206 1937 Size: 1269 4594 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2424 2514 3926 5310 5578 1632 Size: 792 90 1412 1384 268 1917 Fragments arranged by size: 1917 1412 1384 792 268 90 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 4762 5739 1203 Size: 3559 977 1327 Fragments arranged by size: 3559 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 4592 4592 Size: 5863 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2554 1184 Size: 1370 4493 AlwNI CAG_nnn'CTG Cuts at: 3101 3606 5178 3101 Size: 505 1572 3786 Fragments arranged by size: 3786 1572 505 ApaLI G'TGCA_C Cuts at: 3135 3920 5076 3135 Size: 785 1156 3922 Fragments arranged by size: 3922 1156 785 ApoI r'AATT_y Cuts at: 1493 3172 3398 3550 5659 1493 Size: 1679 226 152 2109 1697 Fragments arranged by size: 2109 1697 1679 226 152 AseI AT'TA_AT Cuts at: 160 1032 2223 4417 160 Size: 872 1191 2194 1606 Fragments arranged by size: 2194 1606 1191 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 3188 1870 Size: 73 1245 4545 Fragments arranged by size: 4545 1245 73 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3184 4639 618 Size: 325 217 627 1397 1455 1842 Fragments arranged by size: 1842 1455 1397 627 325 217 BanII G_rGCy'C Cuts at: 1391 2102 3182 1391 Size: 711 1080 4072 Fragments arranged by size: 4072 1080 711 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2190 928 Size: 971 291 4601 Fragments arranged by size: 4601 971 291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4054 4088 1903 Size: 34 2117 34 3678 Fragments arranged by size: 3678 2117 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3755 4965 3755 Size: 1210 4653 BclI T'GATC_A Cuts at: 1548 2332 1548 Size: 784 5079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2293 4474 Size: 107 122 71 631 605 337 284 2181 Cuts at: 4474 136 Size: 1525 Fragments arranged by size: 2181 1525 631 605 337 284 122 107 71 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 3139 3924 5080 1790 Size: 1349 785 1156 2573 Fragments arranged by size: 2573 1349 1156 785 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3594 4552 442 Size: 416 53 83 399 24 2177 958 1753 Fragments arranged by size: 2177 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 4523 1239 Size: 809 2475 2579 Fragments arranged by size: 2579 2475 809 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3988 4853 5151 3988 Size: 865 298 4700 Fragments arranged by size: 4700 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4526 882 Size: 617 3027 2219 Fragments arranged by size: 3027 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3654 493 Size: 711 2450 2702 Fragments arranged by size: 2702 2450 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3670 4052 4687 275 Size: 53 83 186 936 2137 382 635 1451 Fragments arranged by size: 2137 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4296 4694 4968 5115 5526 4296 Size: 398 274 147 411 4633 Fragments arranged by size: 4633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5638 Fragments arranged by size: 5638 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5863 BseYI C'CCAG_C Cuts at: 2086 5066 5562 2086 Size: 2980 496 2387 Fragments arranged by size: 2980 2387 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3313 4074 5102 1540 Size: 275 186 12 1300 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2740 3081 3139 3924 4009 5080 2740 Size: 341 58 785 85 1071 3523 Fragments arranged by size: 3523 1071 785 341 85 58 BsmI GAATG_Cn' Cuts at: 3374 3467 3374 Size: 93 5770 BsmBI CGTCTCn'nnnn_ Cuts at: 2012 3045 2012 Size: 1033 4830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2740 3081 3139 3182 3924 4009 Size: 399 312 638 341 58 43 742 85 Cuts at: 4009 5080 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 638 399 341 312 85 58 43 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 3752 5482 3752 Size: 1730 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2231 3218 844 Size: 1387 987 3489 Fragments arranged by size: 3489 1387 987 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2686 4358 4532 65 Size: 1183 1438 1672 174 1396 Fragments arranged by size: 1672 1438 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2465 4507 4694 1700 Size: 110 655 2042 187 2869 Fragments arranged by size: 2869 2042 655 187 110 BsrGI T'GTAC_A Cuts at: 96 2512 96 Size: 2416 3447 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 2528 794 Size: 1734 4129 BstBI TT'CG_AA Cuts at: 2869 4667 2869 Size: 1798 4065 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5863 BstXI CCAn_nnnn'nTGG Cuts at: 2852 3033 2852 Size: 181 5682 BstYI r'GATC_y Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858 Size: 1221 367 385 17 1452 86 12 346 Cuts at: 5858 1972 Size: 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2341 2341 Size: 5863 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2234 2611 513 Size: 552 939 9 221 377 3765 Fragments arranged by size: 3765 939 552 377 221 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5124 Fragments arranged by size: 5124 683 56 BtsI GCAGTG_nn' Cuts at: 2056 2796 3379 4173 4193 2056 Size: 740 583 794 20 3726 Fragments arranged by size: 3726 794 740 583 20 ClaI AT'CG_AT Cuts at: 1554 2248 1554 Size: 694 5169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 2128 3167 2128 Size: 1039 4824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4870 809 Size: 507 3554 1802 Fragments arranged by size: 3554 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 3023 3310 Size: 54 1425 50 275 18 180 1013 287 Cuts at: 3310 4199 5828 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1013 889 287 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 3310 1537 Size: 275 198 1300 4090 Fragments arranged by size: 4090 1300 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 3793 1353 Size: 2440 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3018 4450 4824 4970 387 Size: 1064 138 1429 1432 374 146 1280 Fragments arranged by size: 1432 1429 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2424 2514 3926 4523 5310 5578 Size: 393 416 376 90 1412 597 787 268 Cuts at: 5578 1239 Size: 1524 Fragments arranged by size: 1524 1412 787 597 416 393 376 268 90 EcoO109I rG'GnC_Cy Cuts at: 2631 2695 2631 Size: 64 5799 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 EcoRV GAT'ATC Cuts at: 2665 2665 Size: 5863 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 5010 1678 Size: 3332 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4504 4536 4578 4610 1373 Size: 32 135 32 2932 32 42 32 2626 Fragments arranged by size: 2932 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3207 3453 669 Size: 988 281 1269 246 3079 Fragments arranged by size: 3079 1269 988 281 246 HindIII A'AGCT_T Cuts at: 748 2897 748 Size: 2149 3714 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 2979 3462 2979 Size: 483 5380 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2698 2929 3014 4977 5161 1305 Size: 1393 231 85 1963 184 2007 Fragments arranged by size: 2007 1963 1393 231 184 85 MscI TGG'CCA Cuts at: 10 64 1489 1832 3025 10 Size: 54 1425 343 1193 2848 Fragments arranged by size: 2848 1425 1193 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2764 3821 4180 4339 518 Size: 648 1598 1057 359 159 2042 Fragments arranged by size: 2042 1598 1057 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3279 3956 5104 5349 1606 Size: 1673 677 1148 245 2120 Fragments arranged by size: 2120 1673 1148 677 245 NaeI GCC'GGC Cuts at: 2467 2467 Size: 5863 NcoI C'CATG_G Cuts at: 513 1065 2004 2234 2611 513 Size: 552 939 230 377 3765 Fragments arranged by size: 3765 939 552 377 230 NdeI CA'TA_TG Cuts at: 387 2761 387 Size: 2374 3489 NgoMIV G'CCGG_C Cuts at: 2465 2465 Size: 5863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 2526 2843 3221 4766 1132 Size: 97 1297 317 378 1545 2229 Fragments arranged by size: 2229 1545 1297 378 317 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5863 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5863 PfoI T'CCnGG_A Cuts at: 2201 2201 Size: 5863 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2695 2695 Size: 5863 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 2565 3433 2565 Size: 868 4995 PstI C_TGCA'G Cuts at: 830 2048 3215 830 Size: 1218 1167 3478 Fragments arranged by size: 3478 1218 1167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SexAI A'CCwGG_T Cuts at: 2479 2479 Size: 5863 SfcI C'TryA_G Cuts at: 826 1045 2044 3211 4346 5027 5218 826 Size: 219 999 1167 1135 681 191 1471 Fragments arranged by size: 1471 1167 1135 999 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 3190 1872 Size: 1318 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3967 4868 5130 820 Size: 197 926 2024 901 262 1553 Fragments arranged by size: 2024 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 2843 3221 1132 Size: 1711 378 3774 Fragments arranged by size: 3774 1711 378 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5863 StyI C'CwwG_G Cuts at: 513 1065 2004 2234 2611 3302 513 Size: 552 939 230 377 691 3074 Fragments arranged by size: 3074 939 691 552 377 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3890 3907 4060 4245 4690 325 Size: 1255 2310 17 153 185 445 1498 Fragments arranged by size: 2310 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2512 4109 5821 Size: 274 80 33 51 1443 535 1597 1712 Cuts at: 5821 96 Size: 138 Fragments arranged by size: 1712 1597 1443 535 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5823 5855 5823 Size: 32 5831 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5863 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 3188 1870 Size: 1318 4545 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI