Restriction map for
pFN21AA0094
(Linear) (Six-base) MAPSORT of: KIAA0094.seq Check: 3553 from: 1 to: 1158
KIAA0094.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 509 1158
Size: 419 90 649
Fragments arranged by size:
649 419 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 549 1158
Size: 549 609
AlwNI CAG_nnn'CTG
Cuts at: 0 1096 1158
Size: 1096 62
ApaLI G'TGCA_C
Cuts at: 0 1130 1158
Size: 1130 28
AseI AT'TA_AT
Cuts at: 0 218 1158
Size: 218 940
BanII G_rGCy'C
Cuts at: 0 97 1158
Size: 97 1061
BbsI GAAGACnn'nnnn_
Cuts at: 0 185 1158
Size: 185 973
BclI T'GATC_A
Cuts at: 0 327 1158
Size: 327 831
BglI GCCn_nnn'nGGC
Cuts at: 0 288 1158
Size: 288 870
Bme1580I G_kGCm'C
Cuts at: 0 1134 1158
Size: 1134 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1158
Size: 43 1115
BseYI C'CCAG_C
Cuts at: 0 81 1158
Size: 81 1077
BsiEI CG_ry'CG
Cuts at: 0 8 1158
Size: 8 1150
BsiHKAI G_wGCw'C
Cuts at: 0 735 1076 1134 1158
Size: 735 341 58 24
Fragments arranged by size:
735 341 58 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 7 1040 1158
Size: 7 1033 118
Fragments arranged by size:
1033 118 7
Bsp1286I G_dGCh'C
Cuts at: 0 97 735 1076 1134 1158
Size: 97 638 341 58 24
Fragments arranged by size:
638 341 97 58 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 226 1158
Size: 226 932
BsrDI GCAATG_nn'
Cuts at: 0 681 1158
Size: 681 477
BsrFI r'CCGG_y
Cuts at: 0 460 1158
Size: 460 698
BsrGI T'GTAC_A
Cuts at: 0 507 1158
Size: 507 651
BstAPI GCAn_nnn'nTGC
Cuts at: 0 523 1158
Size: 523 635
BstBI TT'CG_AA
Cuts at: 0 864 1158
Size: 864 294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 847 1028 1158
Size: 847 181 130
Fragments arranged by size:
847 181 130
Bsu36I CC'TnA_GG
Cuts at: 0 336 1158
Size: 336 822
BtgI C'CryG_G
Cuts at: 0 8 229 606 1158
Size: 8 221 377 552
Fragments arranged by size:
552 377 221 8
BtsI GCAGTG_nn'
Cuts at: 0 51 791 1158
Size: 51 740 367
Fragments arranged by size:
740 367 51
ClaI AT'CG_AT
Cuts at: 0 243 1158
Size: 243 915
DraI TTT'AAA
Cuts at: 0 123 1158
Size: 123 1035
EaeI y'GGCC_r
Cuts at: 0 5 1018 1158
Size: 5 1013 140
Fragments arranged by size:
1013 140 5
EagI C'GGCC_G
Cuts at: 0 5 1158
Size: 5 1153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1013 1158
Size: 1013 145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 419 509 1158
Size: 43 376 90 649
Fragments arranged by size:
649 376 90 43
EcoO109I rG'GnC_Cy
Cuts at: 0 626 690 1158
Size: 626 64 468
Fragments arranged by size:
626 468 64
EcoRV GAT'ATC
Cuts at: 0 660 1158
Size: 660 498
HindIII A'AGCT_T
Cuts at: 0 892 1158
Size: 892 266
MfeI C'AATT_G
Cuts at: 0 974 1158
Size: 974 184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 924 1009 1158
Size: 693 231 85 149
Fragments arranged by size:
693 231 149 85
MscI TGG'CCA
Cuts at: 0 1020 1158
Size: 1020 138
MslI CAynn'nnrTG
Cuts at: 0 759 1158
Size: 759 399
NaeI GCC'GGC
Cuts at: 0 462 1158
Size: 462 696
NcoI C'CATG_G
Cuts at: 0 229 606 1158
Size: 229 377 552
Fragments arranged by size:
552 377 229
NdeI CA'TA_TG
Cuts at: 0 756 1158
Size: 756 402
NgoMIV G'CCGG_C
Cuts at: 0 460 1158
Size: 460 698
NspI r_CATG'y
Cuts at: 0 521 838 1158
Size: 521 317 320
Fragments arranged by size:
521 320 317
PfoI T'CCnGG_A
Cuts at: 0 196 1158
Size: 196 962
PpuMI rG'GwC_Cy
Cuts at: 0 690 1158
Size: 690 468
PsiI TTA'TAA
Cuts at: 0 560 1158
Size: 560 598
PstI C_TGCA'G
Cuts at: 0 43 1158
Size: 43 1115
SexAI A'CCwGG_T
Cuts at: 0 474 1158
Size: 474 684
SfcI C'TryA_G
Cuts at: 0 39 1158
Size: 39 1119
SphI G_CATG'C
Cuts at: 0 838 1158
Size: 838 320
StyI C'CwwG_G
Cuts at: 0 229 606 1158
Size: 229 377 552
Fragments arranged by size:
552 377 229
TatI w'GTAC_w
Cuts at: 0 507 1158
Size: 507 651
Enzymes that do cut:
AcuI AlfI AlwNI ApaLI AseI BanII BbsI BclI
BglI Bme1580I BpmI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstXI Bsu36I
BtgI BtsI ClaI DraI EaeI EagI EciI Eco57MI
EcoO109I EcoRV HindIII MfeI MmeI MscI MslI NaeI
NcoI NdeI NgoMIV NspI PfoI PpuMI PsiI PstI
SexAI SfcI SphI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApoI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsgI BsiWI BsmI BspEI BspHI BsrBI BssHII
BssSI BstEII BstYI BstZ17I BtgZI CspCI DraIII DrdI
EarI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MluI MspA1I
NarI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI