(Circular) (Six-base) MAPSORT of: pFN21AA0090.seq Check: 6992 from: 1 to: 7681 pFN21AA0090 7681 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4590 4590 Size: 7681 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5491 278 Size: 53 83 186 936 3955 2468 Fragments arranged by size: 3955 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2070 4842 5024 1937 Size: 133 2772 182 4594 Fragments arranged by size: 4594 2772 182 133 Acc65I G'GTAC_C Cuts at: 5002 5002 Size: 7681 AclI AA'CG_TT Cuts at: 1563 5808 6181 1563 Size: 4245 373 3063 Fragments arranged by size: 4245 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2462 2569 3066 3878 4450 4889 5744 7128 Size: 830 107 497 812 572 439 855 1384 Cuts at: 7128 7396 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 855 830 812 572 497 439 268 107 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7484 AflIII A'CryG_T Cuts at: 1203 4887 6580 7557 1203 Size: 3684 1693 977 1327 Fragments arranged by size: 3684 1693 1327 977 AgeI A'CCGG_T Cuts at: 6512 6512 Size: 7681 AhdI GACnn_n'nnGTC Cuts at: 3784 6410 3784 Size: 2626 5055 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7681 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7681 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2616 2648 2616 Size: 32 7649 AlwNI CAG_nnn'CTG Cuts at: 2671 5424 6996 2671 Size: 2753 1572 3356 Fragments arranged by size: 3356 2753 1572 ApaI G_GGCC'C Cuts at: 2569 2872 2569 Size: 303 7378 ApaLI G'TGCA_C Cuts at: 3760 5738 6894 3760 Size: 1978 1156 4547 Fragments arranged by size: 4547 1978 1156 ApoI r'AATT_y Cuts at: 1493 2141 2203 2804 3416 3587 4990 5216 5368 Size: 648 62 601 612 171 1403 226 152 Cuts at: 5368 7477 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1403 648 612 601 226 171 152 62 AseI AT'TA_AT Cuts at: 160 1032 6235 160 Size: 872 5203 1606 Fragments arranged by size: 5203 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7681 AvaI C'yCGr_G Cuts at: 1870 1943 2722 2826 2865 4649 4780 4836 5006 Size: 73 779 104 39 1784 131 56 170 Cuts at: 5006 1870 Size: 4545 Fragments arranged by size: 4545 1784 779 170 131 104 73 56 39 BamHI G'GATC_C Cuts at: 2153 4636 5011 5378 2153 Size: 2483 375 367 4456 Fragments arranged by size: 4456 2483 375 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2229 2481 3659 5002 6457 Size: 325 217 627 442 252 1178 1343 1455 Cuts at: 6457 618 Size: 1842 Fragments arranged by size: 1842 1455 1343 1178 627 442 325 252 217 BanII G_rGCy'C Cuts at: 1391 2569 2729 2872 4431 5000 1391 Size: 1178 160 143 1559 569 4072 Fragments arranged by size: 4072 1559 1178 569 160 143 BbeI G_GCGC'C Cuts at: 2233 2233 Size: 7681 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2082 2453 2999 3802 928 Size: 971 183 371 546 803 4807 Fragments arranged by size: 4807 971 803 546 371 183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5872 5906 1903 Size: 34 3935 34 3678 Fragments arranged by size: 3935 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5573 6783 5573 Size: 1210 6471 BclI T'GATC_A Cuts at: 1548 2295 4932 1548 Size: 747 2637 4297 Fragments arranged by size: 4297 2637 747 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3211 4544 Size: 107 122 71 631 605 337 1202 1333 Cuts at: 4544 6292 136 Size: 1748 1525 Fragments arranged by size: 1748 1525 1333 1202 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2220 7676 2220 Size: 5456 2225 BlpI GC'TnA_GC Cuts at: 5098 5098 Size: 7681 Bme1580I G_kGCm'C Cuts at: 1790 2249 2484 2569 2872 3397 3764 4442 5742 Size: 459 235 85 303 525 367 678 1300 Cuts at: 5742 6898 1790 Size: 1156 2573 Fragments arranged by size: 2573 1300 1156 678 525 459 367 303 235 85 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7681 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2223 2368 2846 Size: 416 53 83 399 24 806 145 478 Cuts at: 2846 2866 2994 3399 3845 3884 4308 4378 5412 Size: 20 128 405 446 39 424 70 1034 Cuts at: 5412 6370 442 Size: 958 1753 Fragments arranged by size: 1753 1034 958 806 478 446 424 416 405 399 145 128 83 70 53 39 24 20 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7681 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3216 3670 3216 Size: 454 7227 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2458 2697 2774 3581 4445 4487 4623 4683 Size: 1219 239 77 807 864 42 136 60 Cuts at: 4683 4808 6341 1239 Size: 125 1533 2579 Fragments arranged by size: 2579 1533 1219 864 807 239 136 125 77 60 42 Bpu10I CC'TnA_GC Cuts at: 3207 3207 Size: 7681 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 5806 6671 6969 2471 Size: 3335 865 298 3183 Fragments arranged by size: 3335 3183 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6344 882 Size: 617 4845 2219 Fragments arranged by size: 4845 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4888 5472 493 Size: 711 3684 584 2702 Fragments arranged by size: 3684 2702 711 584 BsaBI GATnn'nnATC Cuts at: 7675 7675 Size: 7681 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2230 4293 5488 5870 Size: 53 83 186 936 697 2063 1195 382 Cuts at: 5870 6505 275 Size: 635 1451 Fragments arranged by size: 2063 1451 1195 936 697 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6114 6512 6786 6933 7344 6114 Size: 398 274 147 411 6451 Fragments arranged by size: 6451 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2210 2240 2445 2475 2616 Size: 30 165 30 807 30 205 30 141 Cuts at: 2616 2646 2721 2751 1178 Size: 30 75 30 6108 Fragments arranged by size: 6108 807 205 165 141 75 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3353 3694 4306 4691 1186 Size: 2167 341 612 385 4176 Fragments arranged by size: 4176 2167 612 385 341 BseYI C'CCAG_C Cuts at: 2841 3442 4485 6884 7380 2841 Size: 601 1043 2399 496 3142 Fragments arranged by size: 3142 2399 1043 601 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 2867 3290 4343 1163 Size: 573 528 603 423 1053 4501 Fragments arranged by size: 4501 1053 603 573 528 423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2064 5131 5892 6920 1540 Size: 275 186 63 3067 761 1028 2301 Fragments arranged by size: 3067 2301 1028 761 275 186 63 BsiHKAI G_wGCw'C Cuts at: 2729 3764 4155 4197 4244 4431 5742 5827 6898 Size: 1035 391 42 47 187 1311 85 1071 Cuts at: 6898 2729 Size: 3512 Fragments arranged by size: 3512 1311 1071 1035 391 187 85 47 42 BsmI GAATG_Cn' Cuts at: 4357 4761 5192 5285 4357 Size: 404 431 93 6753 Fragments arranged by size: 6753 431 404 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2750 3474 2750 Size: 724 6957 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2249 2484 2569 2729 2872 3397 3764 Size: 399 459 235 85 160 143 525 367 Cuts at: 3764 4155 4197 4244 4431 4442 5000 5742 5827 Size: 391 42 47 187 11 558 742 85 Cuts at: 5827 6898 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 558 525 459 399 391 367 235 187 160 143 85 85 47 42 11 BspEI T'CCGG_A Cuts at: 7344 7344 Size: 7681 BspHI T'CATG_A Cuts at: 5570 7300 5570 Size: 1730 5951 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2629 4227 4590 5036 844 Size: 1785 1598 363 446 3489 Fragments arranged by size: 3489 1785 1598 446 363 BsrBI CCG'CTC Cuts at: 1394 1823 3195 3211 5568 1394 Size: 429 1372 16 2357 3507 Fragments arranged by size: 3507 2357 1372 429 16 BsrDI GCAATG_nn' Cuts at: 65 1248 6176 6350 65 Size: 1183 4928 174 1396 Fragments arranged by size: 4928 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6325 6512 1700 Size: 110 4515 187 2869 Fragments arranged by size: 4515 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4705 96 Size: 4609 3072 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7681 BssSI C'ACGA_G Cuts at: 3137 5741 6753 3137 Size: 2604 1012 4065 Fragments arranged by size: 4065 2604 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3880 794 Size: 3086 4595 BstBI TT'CG_AA Cuts at: 4060 6485 4060 Size: 2425 5256 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7681 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2396 2854 4222 4801 2164 Size: 232 458 1368 579 5044 Fragments arranged by size: 5044 1368 579 458 232 BstYI r'GATC_y Cuts at: 1972 2153 2220 2778 4066 4636 4653 5011 5378 Size: 181 67 558 1288 570 17 358 367 Cuts at: 5378 5763 5780 7232 7318 7330 7676 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1288 570 558 385 367 358 346 181 86 67 17 17 12 Bsu36I CC'TnA_GG Cuts at: 3625 4623 3625 Size: 998 6683 BtgI C'CryG_G Cuts at: 513 1065 2004 2064 2657 4227 4539 513 Size: 552 939 60 593 1570 312 3655 Fragments arranged by size: 3655 1570 939 593 552 312 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2416 487 Size: 683 56 1190 5752 Fragments arranged by size: 5752 1190 683 56 BtsI GCAGTG_nn' Cuts at: 2783 4153 4195 5197 5991 6011 2783 Size: 1370 42 1002 794 20 4453 Fragments arranged by size: 4453 1370 1002 794 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7681 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7646 DraI TTT'AAA Cuts at: 3712 4985 3712 Size: 1273 6408 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6688 809 Size: 507 5372 1802 Fragments arranged by size: 5372 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 3576 4224 Size: 54 1425 50 275 18 231 1515 648 Cuts at: 4224 5128 6017 7646 8 Size: 904 889 1629 43 Fragments arranged by size: 1629 1515 1425 904 889 648 275 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2061 5128 1537 Size: 275 249 3067 4090 Fragments arranged by size: 4090 3067 275 249 EarI CTCTTCn'nnn_ Cuts at: 1353 5611 1353 Size: 4258 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6268 6642 6788 387 Size: 1064 138 4679 374 146 1280 Fragments arranged by size: 4679 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2727 4429 2727 Size: 1702 5979 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2458 2462 2569 2697 2774 3066 3581 Size: 393 826 4 107 128 77 292 515 Cuts at: 3581 3878 4445 4450 4487 4623 4683 4808 4889 Size: 297 567 5 37 136 60 125 81 Cuts at: 4889 5744 6341 7128 7396 1239 Size: 855 597 787 268 1524 Fragments arranged by size: 1524 855 826 787 597 567 515 393 297 292 268 136 128 125 107 81 77 60 37 5 4 EcoNI CCTnn'n_nnAGG Cuts at: 4622 4622 Size: 7681 EcoO109I rG'GnC_Cy Cuts at: 2350 2566 2830 4340 4498 2350 Size: 216 264 1510 158 5533 Fragments arranged by size: 5533 1510 264 216 158 EcoRI G'AATT_C Cuts at: 3587 4990 5368 3587 Size: 1403 378 5900 Fragments arranged by size: 5900 1403 378 EcoRV GAT'ATC Cuts at: 4052 4052 Size: 7681 FspI TGC'GCA Cuts at: 6187 6187 Size: 7681 HaeII r_GCGC'y Cuts at: 1678 2233 3865 6828 1678 Size: 555 1632 2963 2531 Fragments arranged by size: 2963 2531 1632 555 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 2711 2743 3265 Size: 32 135 32 732 32 375 32 522 Cuts at: 3265 3297 3770 3802 4735 4767 6322 6354 6396 Size: 32 473 32 933 32 1555 32 42 Cuts at: 6396 6428 1373 Size: 32 2626 Fragments arranged by size: 2626 1555 933 732 522 473 375 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2239 5025 5271 669 Size: 988 281 301 2786 246 3079 Fragments arranged by size: 3079 2786 988 301 281 246 HindIII A'AGCT_T Cuts at: 748 3632 748 Size: 2884 4797 HpaI GTT'AAC Cuts at: 5271 5271 Size: 7681 KasI G'GCGC_C Cuts at: 2229 2229 Size: 7681 KpnI G_GTAC'C Cuts at: 5006 5006 Size: 7681 MfeI C'AATT_G Cuts at: 5280 5280 Size: 7681 MluI A'CGCG_T Cuts at: 7557 7557 Size: 7681 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2845 2999 6795 6979 1305 Size: 1540 154 3796 184 2007 Fragments arranged by size: 3796 2007 1540 184 154 MscI TGG'CCA Cuts at: 10 64 1489 1832 3578 10 Size: 54 1425 343 1746 4113 Fragments arranged by size: 4113 1746 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5639 5998 6157 518 Size: 648 4473 359 159 2042 Fragments arranged by size: 4473 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2066 4117 4489 5097 5774 6922 7167 1606 Size: 460 2051 372 608 677 1148 245 2120 Fragments arranged by size: 2120 2051 1148 677 608 460 372 245 NarI GG'CG_CC Cuts at: 2230 2230 Size: 7681 NcoI C'CATG_G Cuts at: 513 1065 2004 4539 513 Size: 552 939 2535 3655 Fragments arranged by size: 3655 2535 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7681 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7681 NotI GC'GGCC_GC Cuts at: 2061 5128 2061 Size: 3067 4614 NspI r_CATG'y Cuts at: 1132 1229 5039 6584 1132 Size: 97 3810 1545 2229 Fragments arranged by size: 3810 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4165 1348 Size: 2817 4864 PciI A'CATG_T Cuts at: 6580 6580 Size: 7681 PflMI CCAn_nnn'nTGG Cuts at: 1108 2746 3669 1108 Size: 1638 923 5120 Fragments arranged by size: 5120 1638 923 PmeI GTTT'AAAC Cuts at: 4985 4985 Size: 7681 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2616 2648 2716 2748 3721 3753 5750 5782 7263 Size: 32 68 32 973 32 1997 32 1481 Cuts at: 7263 7295 2616 Size: 32 3002 Fragments arranged by size: 3002 1997 1481 973 68 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2830 4498 2830 Size: 1668 6013 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7681 PsiI TTA'TAA Cuts at: 5251 5251 Size: 7681 PspOMI G'GGCC_C Cuts at: 2565 2868 2565 Size: 303 7378 PspXI vC'TCGA_Gb Cuts at: 4836 4836 Size: 7681 PstI C_TGCA'G Cuts at: 830 2415 2667 2790 2916 3007 3303 5033 830 Size: 1585 252 123 126 91 296 1730 3478 Fragments arranged by size: 3478 1730 1585 296 252 126 123 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7681 PvuII CAG'CTG Cuts at: 4489 4489 Size: 7681 SacI G_AGCT'C Cuts at: 2729 4431 2729 Size: 1702 5979 SacII CC_GC'GG Cuts at: 2067 2067 Size: 7681 SalI G'TCGA_C Cuts at: 1936 5023 1936 Size: 3087 4594 SbfI CC_TGCA'GG Cuts at: 2415 5033 2415 Size: 2618 5063 ScaI AGT'ACT Cuts at: 4385 5929 7641 4385 Size: 1544 1712 4425 Fragments arranged by size: 4425 1712 1544 SfcI C'TryA_G Cuts at: 826 1045 2411 2663 2786 2912 3003 3299 5029 Size: 219 1366 252 123 126 91 296 1730 Cuts at: 5029 6164 6845 7036 826 Size: 1135 681 191 1471 Fragments arranged by size: 1730 1471 1366 1135 681 296 252 219 191 126 123 91 SfoI GGC'GCC Cuts at: 2231 2231 Size: 7681 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7681 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7681 SmaI CCC'GGG Cuts at: 1872 2828 2867 5008 1872 Size: 956 39 2141 4545 Fragments arranged by size: 4545 2141 956 39 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 4836 5785 6686 6948 820 Size: 197 926 543 2350 949 901 262 1553 Fragments arranged by size: 2350 1553 949 926 901 543 262 197 SnaBI TAC'GTA Cuts at: 493 5472 493 Size: 4979 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7681 SphI G_CATG'C Cuts at: 1132 5039 1132 Size: 3907 3774 SspI AAT'ATT Cuts at: 5 52 5605 5 Size: 47 5553 2081 Fragments arranged by size: 5553 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7681 StyI C'CwwG_G Cuts at: 513 1065 2004 2136 3912 4395 4539 5120 513 Size: 552 939 132 1776 483 144 581 3074 Fragments arranged by size: 3074 1776 939 581 552 483 144 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2482 4360 5708 5725 5878 6063 6508 Size: 1255 902 1878 1348 17 153 185 445 Cuts at: 6508 325 Size: 1498 Fragments arranged by size: 1878 1498 1348 1255 902 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4111 4383 4705 Size: 274 80 33 51 1443 2134 272 322 Cuts at: 4705 5927 7639 96 Size: 1222 1712 138 Fragments arranged by size: 2134 1712 1443 1222 322 274 272 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2210 2242 2277 2309 2445 2477 2721 2753 3940 Size: 32 35 32 136 32 244 32 1187 Cuts at: 3940 3972 4145 4177 7641 7673 2210 Size: 32 173 32 3464 32 2218 Fragments arranged by size: 3464 2218 1187 244 173 136 35 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7681 XbaI T'CTAG_A Cuts at: 5017 5017 Size: 7681 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4537 4537 Size: 7681 XhoI C'TCGA_G Cuts at: 1943 4836 1943 Size: 2893 4788 XmaI C'CCGG_G Cuts at: 1870 2826 2865 5006 1870 Size: 956 39 2141 4545 Fragments arranged by size: 4545 2141 956 39 XmnI GAAnn'nnTTC Cuts at: 3796 5810 3796 Size: 2014 5667 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5489 276 Size: 53 83 186 936 3955 2468 Fragments arranged by size: 3955 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI