Restriction map for
pFN21AA0060
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HinfI HpaI KasI KpnI KspI
MfeI MflI MluI MroI MspA1I
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tsp509I XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 334 GTCGAC GT^[AC][GT]AC
AflIII 665 ACATGT A^C[GA][CT]GT
AspI 672 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 233 CTCGAG C^[CT]CG[GA]G
BanI 540 GGTGCC G^G[CT][GA]CC
BanII 112 GGGCTC G[GA]GC[CT]^C
BciVI 492 GGATAC GTATCC(6/5)
BcnI 243 CCCGG CC^[GC]GG
BsaAI 220 TACGTG [CT]AC^GT[GA]
BsaBI 600 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 236 GAGACC GGTCTC(1/5)
BseRI 649 GAGGAG GAGGAG(10/8)
BsmAI 236 GAGAC GTCTC(1/5)
BsmFI 176 GGGAC GGGAC(10/14)
BsmI 327 GAATGC GAATGC(1/-1)
BsoBI 233 CTCGAG C^[CT]CG[GA]G
BspLU11I 665 ACATGT A^CATGT
BspMI 382 GCAGGT ACCTGC(4/8)
BsrDI 429 CATTGC GCAATG(2/0)
BstBI 846 TTCGAA TT^CGAA
DraI 187 TTTAAA TTT^AAA
EarI 364 GAAGAG CTCTTC(1/4)
FauI 698 CCCGC CCCGC(4/6)
HapII 244 CCGG C^CGG
HgaI 480 GACGC GACGC(5/10)
HincII 334 GTCGAC GT[CT]^[GA]AC
HindII 334 GTCGAC GT[CT]^[GA]AC
HpaII 244 CCGG C^CGG
Ksp632I 364 GAAGAG CTCTTC(1/4)
MaeII 221 ACGT A^CGT
MamI 600 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 244 CCGG C^CGG
MwoI 616 GCTCACAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 243 CCCGG CC^[GC]GG
NspI 665 ACATGT [GA]CATG^[CT]
NspV 846 TTCGAA TT^CGAA
PaeR7I 233 CTCGAG C^TCGAG
PflFI 672 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 312 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 380 CTGCAG CTGCA^G
SalI 334 GTCGAC G^TCGAC
ScaI 98 AGTACT AGT^ACT
SfuI 846 TTCGAA TT^CGAA
SmlI 233 CTCGAG C^T[CT][GA]AG
Tsp45I 716 GTGAC ^GT[GC]AC
Tth111I 672 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 312 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 312 CCACATTCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 233 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 699 CCGC CCGC(-3/-1)
46 GCGG
AlwI 600 GATCC GGATC(4/5)
389 GGATC
AspHI 19 GAGCAC G[AT]GC[AT]^C
614 GTGCTC
BalI 485 TGGCCA TGG^CCA
503 TGGCCA
BfaI 512 CTAG C^TAG
587 CTAG
BpmI 450 CTCCAG CTGGAG(16/14)
16 CTGGAG
BsiHKAI 19 GAGCAC G[AT]GC[AT]^C
614 GTGCTC
Bsp1407I 635 TGTACA T^GTACA
806 TGTACA
BsrGI 635 TGTACA T^GTACA
806 TGTACA
Cac8I 34 GCGAGC GC[ACGT]^[ACGT]GC
483 GCTGGC
DdeI 29 CTCAG C^T[ACGT]AG
830 CTGAG
DraII 176 GGGACCT [GA]G^G[ACGT]CC[CT]
466 AGGACCC
DsaI 488 CCATGG C^C[GA][CT]GG
548 CCATGG
EaeI 485 TGGCCA [CT]^GGCC[GA]
503 TGGCCA
EcoO109I 176 GGGACCT [GA]G^G[ACGT]CC[CT]
466 AGGACCC
MaeI 512 CTAG C^TAG
587 CTAG
MaeIII 251 GTTAC ^GT[ACGT]AC
716 GTGAC
MluNI 485 TGGCCA TGG^CCA
503 TGGCCA
MscI 485 TGGCCA TGG^CCA
503 TGGCCA
MslI 534 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
573 CACTGTCATG
NcoI 488 CCATGG C^CATGG
548 CCATGG
PpuMI 176 GGGACCT [GA]G^G[AT]CC[CT]
466 AGGACCC
SfcI 340 CTACAG C^T[GA][CT]AG
380 CTGCAG
SspBI 635 TGTACA T^GTACA
806 TGTACA
TspRI 120 CACTG CA[GC]TG[ACGT][ACGT]^
573 CACTG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI Asp700I AvaII BbsI
BbvI BmyI BpuAI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HphI ItaI
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
Tru9I TseI TthHB8I XmnI