(Circular) (Six-base) MAPSORT of: pFN21AA0060.seq Check: 5128 from: 1 to: 5572 pFN21AA0060 5572 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2378 2378 Size: 5572 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2340 2915 1937 Size: 403 575 4594 Fragments arranged by size: 4594 575 403 Acc65I G'GTAC_C Cuts at: 2893 2893 Size: 5572 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3635 5019 5287 1632 Size: 2003 1384 268 1917 Fragments arranged by size: 2003 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 2670 4471 5448 1203 Size: 1467 1801 977 1327 Fragments arranged by size: 1801 1467 1327 977 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 4301 4301 Size: 5572 AleI CACnn'nnGTG Cuts at: 1166 2543 1166 Size: 1377 4195 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5572 AlwNI CAG_nnn'CTG Cuts at: 3315 4887 3315 Size: 1572 4000 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2881 3107 3259 5368 1493 Size: 1388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1388 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2238 2897 1870 Size: 73 295 659 4545 Fragments arranged by size: 4545 659 295 73 BamHI G'GATC_C Cuts at: 2902 3269 2902 Size: 367 5205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2545 2893 4348 618 Size: 325 217 627 758 348 1455 1842 Fragments arranged by size: 1842 1455 758 627 348 325 217 BanII G_rGCy'C Cuts at: 1391 2121 2891 1391 Size: 730 770 4072 Fragments arranged by size: 4072 770 730 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2210 2489 2759 928 Size: 971 311 279 270 3741 Fragments arranged by size: 3741 971 311 279 270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 1903 Size: 34 1826 34 3678 Fragments arranged by size: 3678 1826 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2491 3464 4674 2491 Size: 973 1210 3389 Fragments arranged by size: 3389 1210 973 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4183 136 Size: 107 122 71 631 605 2511 1525 Fragments arranged by size: 2511 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5567 5567 Size: 5572 BlpI GC'TnA_GC Cuts at: 2989 2989 Size: 5572 Bme1580I G_kGCm'C Cuts at: 1790 2550 2579 3633 4789 1790 Size: 760 29 1054 1156 2573 Fragments arranged by size: 2573 1156 1054 760 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5572 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2136 3303 4261 Size: 416 53 83 399 24 719 1167 958 Cuts at: 4261 442 Size: 1753 Fragments arranged by size: 1753 1167 958 719 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5572 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2470 2470 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2440 4232 1239 Size: 803 398 1792 2579 Fragments arranged by size: 2579 1792 803 398 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3697 4562 4860 3697 Size: 865 298 4409 Fragments arranged by size: 4409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2235 4235 882 Size: 617 736 2000 2219 Fragments arranged by size: 2219 2000 736 617 BsaAI yAC'GTr Cuts at: 493 1204 2227 3363 493 Size: 711 1023 1136 2702 Fragments arranged by size: 2702 1136 1023 711 BsaBI GATnn'nnATC Cuts at: 2609 5566 2609 Size: 2957 2615 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3379 3761 4396 275 Size: 53 83 186 936 1846 382 635 1451 Fragments arranged by size: 1846 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4005 4403 4677 4824 5235 4005 Size: 398 274 147 411 4342 Fragments arranged by size: 4342 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5347 Fragments arranged by size: 5347 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 1186 Size: 1483 4089 BseYI C'CCAG_C Cuts at: 4775 5271 4775 Size: 496 5076 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4999 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3022 3783 4811 1540 Size: 275 186 1021 761 1028 2301 Fragments arranged by size: 2301 1028 1021 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 2623 3633 3718 4789 2028 Size: 595 1010 85 1071 2811 Fragments arranged by size: 2811 1071 1010 595 85 BsmI GAATG_Cn' Cuts at: 2338 3083 3176 2338 Size: 745 93 4734 Fragments arranged by size: 4734 745 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2121 2550 2579 2623 2891 3633 Size: 399 238 93 429 29 44 268 742 Cuts at: 3633 3718 4789 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 268 238 93 85 44 29 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 3461 5191 3461 Size: 1730 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2378 2927 844 Size: 1534 549 3489 Fragments arranged by size: 3489 1534 549 BsrBI CCG'CTC Cuts at: 1394 1823 3459 1394 Size: 429 1636 3507 Fragments arranged by size: 3507 1636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2433 4067 4241 65 Size: 1183 1185 1634 174 1396 Fragments arranged by size: 1634 1396 1185 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4216 4403 1700 Size: 110 2406 187 2869 Fragments arranged by size: 2869 2406 187 110 BsrGI T'GTAC_A Cuts at: 96 2640 2811 96 Size: 2544 171 2857 Fragments arranged by size: 2857 2544 171 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5572 BssSI C'ACGA_G Cuts at: 3632 4644 3632 Size: 1012 4560 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5572 BstBI TT'CG_AA Cuts at: 2852 4376 2852 Size: 1524 4048 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5572 BstYI r'GATC_y Cuts at: 1972 2902 3269 3654 3671 5123 5209 5221 5567 Size: 930 367 385 17 1452 86 12 346 Cuts at: 5567 1972 Size: 1977 Fragments arranged by size: 1977 1452 930 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2493 2553 513 Size: 552 1428 60 3532 Fragments arranged by size: 3532 1428 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2883 487 Size: 683 56 1657 3176 Fragments arranged by size: 3176 1657 683 56 BtsI GCAGTG_nn' Cuts at: 3088 3882 3902 3088 Size: 794 20 4758 Fragments arranged by size: 4758 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2194 2876 2194 Size: 682 4890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4579 809 Size: 507 3263 1802 Fragments arranged by size: 3263 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2490 2508 3019 Size: 54 1425 50 275 18 660 18 511 Cuts at: 3019 3908 5537 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 660 511 275 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3019 1537 Size: 275 1207 4090 Fragments arranged by size: 4090 1207 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2364 3502 1353 Size: 1011 1138 3423 Fragments arranged by size: 3423 1138 1011 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4159 4533 4679 387 Size: 1064 138 2570 374 146 1280 Fragments arranged by size: 2570 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2440 3635 4232 5019 5287 1239 Size: 393 410 398 1195 597 787 268 1524 Fragments arranged by size: 1524 1195 787 597 410 398 393 268 EcoO109I rG'GnC_Cy Cuts at: 2182 2472 2182 Size: 290 5282 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2865 2865 Size: 5572 FspI TGC'GCA Cuts at: 4078 4078 Size: 5572 HaeII r_GCGC'y Cuts at: 1678 4719 1678 Size: 3041 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4213 4245 4287 4319 1373 Size: 32 135 32 2641 32 42 32 2626 Fragments arranged by size: 2641 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2341 2916 3162 669 Size: 988 281 403 575 246 3079 Fragments arranged by size: 3079 988 575 403 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5572 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KpnI G_GTAC'C Cuts at: 2897 2897 Size: 5572 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2389 4686 4870 1305 Size: 1084 2297 184 2007 Fragments arranged by size: 2297 2007 1084 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2492 2510 10 Size: 54 1425 343 660 18 3072 Fragments arranged by size: 3072 1425 660 343 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2543 2582 3530 3889 4048 518 Size: 648 1377 39 948 359 159 2042 Fragments arranged by size: 2042 1377 948 648 359 159 39 MspA1I CmG'CkG Cuts at: 1606 2988 3665 4813 5058 1606 Size: 1382 677 1148 245 2120 Fragments arranged by size: 2120 1382 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2493 2553 513 Size: 552 1428 60 3532 Fragments arranged by size: 3532 1428 552 60 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5572 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2674 2930 4475 1132 Size: 97 1445 256 1545 2229 Fragments arranged by size: 2229 1545 1445 256 97 PasI CC'CwG_GG Cuts at: 1348 2090 2113 1348 Size: 742 23 4807 Fragments arranged by size: 4807 742 23 PciI A'CATG_T Cuts at: 2670 4471 2670 Size: 1801 3771 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 1108 Size: 1215 4357 PfoI T'CCnGG_A Cuts at: 2019 2468 2019 Size: 449 5123 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2182 2472 2182 Size: 290 5282 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PstI C_TGCA'G Cuts at: 830 2389 2924 830 Size: 1559 535 3478 Fragments arranged by size: 3478 1559 535 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 SalI G'TCGA_C Cuts at: 1936 2339 2914 1936 Size: 403 575 4594 Fragments arranged by size: 4594 575 403 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 2105 3820 5532 2105 Size: 1715 1712 2145 Fragments arranged by size: 2145 1715 1712 SfcI C'TryA_G Cuts at: 826 1045 2345 2385 2920 4055 4736 4927 826 Size: 219 1300 40 535 1135 681 191 1471 Fragments arranged by size: 1471 1300 1135 681 535 219 191 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2899 1872 Size: 1027 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2238 3676 4577 4839 820 Size: 197 926 295 1438 901 262 1553 Fragments arranged by size: 1553 1438 926 901 295 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 2493 2541 2553 2736 3011 513 Size: 552 1428 48 12 183 275 3074 Fragments arranged by size: 3074 1428 552 275 183 48 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2319 2330 3599 3616 3769 3954 4399 Size: 1255 739 11 1269 17 153 185 445 Cuts at: 4399 325 Size: 1498 Fragments arranged by size: 1498 1269 1255 739 445 185 153 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 2640 2811 Size: 274 80 33 51 1443 126 537 171 Cuts at: 2811 3818 5530 96 Size: 1007 1712 138 Fragments arranged by size: 1712 1443 1007 537 274 171 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5532 5564 5532 Size: 32 5540 Tth111I GACn'n_nGTC Cuts at: 1316 2680 1316 Size: 1364 4208 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2324 2324 Size: 5572 XhoI C'TCGA_G Cuts at: 1943 2238 1943 Size: 295 5277 XmaI C'CCGG_G Cuts at: 1870 2897 1870 Size: 1027 4545 XmnI GAAnn'nnTTC Cuts at: 2105 2207 2279 3701 2105 Size: 102 72 1422 3976 Fragments arranged by size: 3976 1422 102 72 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI