(Circular) (Six-base) MAPSORT of: pFN21AB9867.seq Check: 9977 from: 1 to: 6043 pFN21AB9867 6043 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2172 2172 Size: 6043 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3294 3853 278 Size: 53 83 186 936 1758 559 2468 Fragments arranged by size: 2468 1758 936 559 186 83 53 AccI GT'mk_AC Cuts at: 1937 3386 1937 Size: 1449 4594 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2837 4106 5490 5758 1632 Size: 1205 1269 1384 268 1917 Fragments arranged by size: 1917 1384 1269 1205 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 2946 4942 5919 1203 Size: 1743 1996 977 1327 Fragments arranged by size: 1996 1743 1327 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6043 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6043 AlwNI CAG_nnn'CTG Cuts at: 2255 3786 5358 2255 Size: 1531 1572 2940 Fragments arranged by size: 2940 1572 1531 ApaLI G'TGCA_C Cuts at: 4100 5256 4100 Size: 1156 4887 ApoI r'AATT_y Cuts at: 1493 3352 3578 3730 5839 1493 Size: 1859 226 152 2109 1697 Fragments arranged by size: 2109 1859 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 3368 1870 Size: 73 1425 4545 Fragments arranged by size: 4545 1425 73 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3364 4819 618 Size: 325 217 627 1577 1455 1842 Fragments arranged by size: 1842 1577 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3194 3362 1391 Size: 1803 168 4072 Fragments arranged by size: 4072 1803 168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2825 928 Size: 971 926 4146 Fragments arranged by size: 4146 971 926 BbvCI CC'TCA_GC Cuts at: 2573 2573 Size: 6043 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4234 4268 1903 Size: 34 2297 34 3678 Fragments arranged by size: 3678 2297 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2084 3935 5145 2084 Size: 1851 1210 2982 Fragments arranged by size: 2982 1851 1210 BclI T'GATC_A Cuts at: 1548 2352 2433 2832 1548 Size: 804 81 399 4759 Fragments arranged by size: 4759 804 399 81 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4654 136 Size: 107 122 71 631 605 2982 1525 Fragments arranged by size: 2982 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2212 6038 2212 Size: 3826 2217 BlpI GC'TnA_GC Cuts at: 2486 3460 2486 Size: 974 5069 Bme1580I G_kGCm'C Cuts at: 1790 4104 5260 1790 Size: 2314 1156 2573 Fragments arranged by size: 2573 2314 1156 BmgBI CAC'GTC Cuts at: 1318 2947 1318 Size: 1629 4414 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2049 2471 3774 Size: 416 53 83 399 24 632 422 1303 Cuts at: 3774 4732 442 Size: 958 1753 Fragments arranged by size: 1753 1303 958 632 422 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2365 2551 3320 4703 1239 Size: 1126 186 769 1383 2579 Fragments arranged by size: 2579 1383 1126 769 186 Bpu10I CC'TnA_GC Cuts at: 2573 2573 Size: 6043 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4168 5033 5331 4168 Size: 865 298 4880 Fragments arranged by size: 4880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 4706 882 Size: 617 1041 2166 2219 Fragments arranged by size: 2219 2166 1041 617 BsaAI yAC'GTr Cuts at: 493 1204 3834 493 Size: 711 2630 2702 Fragments arranged by size: 2702 2630 711 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3291 3850 4232 4867 Size: 53 83 186 936 1758 559 382 635 Cuts at: 4867 275 Size: 1451 Fragments arranged by size: 1758 1451 936 635 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2391 2770 4476 4874 5148 5295 5706 2391 Size: 379 1706 398 274 147 411 2728 Fragments arranged by size: 2728 1706 411 398 379 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2549 2579 3236 3266 1178 Size: 30 165 30 1146 30 657 30 3955 Fragments arranged by size: 3955 1146 657 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3184 3230 1186 Size: 1998 46 3999 Fragments arranged by size: 3999 1998 46 BseYI C'CCAG_C Cuts at: 3256 5246 5742 3256 Size: 1990 496 3557 Fragments arranged by size: 3557 1990 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 1163 Size: 573 765 4705 Fragments arranged by size: 4705 765 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3493 4254 5282 1540 Size: 275 186 1492 761 1028 2301 Fragments arranged by size: 2301 1492 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2324 4104 4189 5260 2324 Size: 1780 85 1071 3107 Fragments arranged by size: 3107 1780 1071 85 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 BsmBI CGTCTCn'nnnn_ Cuts at: 2903 3074 3298 2903 Size: 171 224 5648 Fragments arranged by size: 5648 224 171 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2324 3194 3362 4104 4189 5260 1391 Size: 399 534 870 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 870 742 534 399 168 85 BspEI T'CCGG_A Cuts at: 5706 5706 Size: 6043 BspHI T'CATG_A Cuts at: 2631 3127 3932 5662 2631 Size: 496 805 1730 3012 Fragments arranged by size: 3012 1730 805 496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 3398 844 Size: 1328 1226 3489 Fragments arranged by size: 3489 1328 1226 BsrBI CCG'CTC Cuts at: 1394 1823 3930 1394 Size: 429 2107 3507 Fragments arranged by size: 3507 2107 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2685 4538 4712 65 Size: 1183 1437 1853 174 1396 Fragments arranged by size: 1853 1437 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4687 4874 1700 Size: 110 2877 187 2869 Fragments arranged by size: 2877 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6043 BssSI C'ACGA_G Cuts at: 3135 4103 5115 3135 Size: 968 1012 4063 Fragments arranged by size: 4063 1012 968 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6043 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 2176 1184 Size: 992 5051 BstXI CCAn_nnnn'nTGG Cuts at: 2119 2539 3241 2119 Size: 420 702 4921 Fragments arranged by size: 4921 702 420 BstYI r'GATC_y Cuts at: 1972 2212 2652 3306 3373 3740 4125 4142 5594 Size: 240 440 654 67 367 385 17 1452 Cuts at: 5594 5680 5692 6038 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 654 440 385 367 346 240 86 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2028 513 Size: 552 963 4528 Fragments arranged by size: 4528 963 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3125 3214 487 Size: 683 56 1899 89 3316 Fragments arranged by size: 3316 1899 683 89 56 BtsI GCAGTG_nn' Cuts at: 2079 2256 2325 3559 4353 4373 2079 Size: 177 69 1234 794 20 3749 Fragments arranged by size: 3749 1234 794 177 69 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3290 5050 809 Size: 507 1974 1760 1802 Fragments arranged by size: 1974 1802 1760 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2625 3490 Size: 54 1425 50 275 18 393 402 865 Cuts at: 3490 4379 6008 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 865 402 393 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3490 1537 Size: 275 1678 4090 Fragments arranged by size: 4090 1678 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3973 1353 Size: 2620 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4630 5004 5150 387 Size: 1064 138 3041 374 146 1280 Fragments arranged by size: 3041 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2365 2551 2837 3320 4106 4703 5490 Size: 393 733 186 286 483 786 597 787 Cuts at: 5490 5758 1239 Size: 268 1524 Fragments arranged by size: 1524 787 786 733 597 483 393 286 268 186 EcoNI CCTnn'n_nnAGG Cuts at: 2647 2698 3209 2647 Size: 51 511 5481 Fragments arranged by size: 5481 511 51 EcoO109I rG'GnC_Cy Cuts at: 2283 3202 2283 Size: 919 5124 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2440 2809 2440 Size: 369 5674 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 5190 1678 Size: 3512 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3129 3161 3236 3268 4684 Size: 32 135 32 1557 32 75 32 1416 Cuts at: 4684 4716 4758 4790 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1557 1416 135 75 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3633 669 Size: 988 281 1449 246 3079 Fragments arranged by size: 3079 1449 988 281 246 HindIII A'AGCT_T Cuts at: 748 2608 748 Size: 1860 4183 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2182 2958 3334 5157 5341 1305 Size: 877 776 376 1823 184 2007 Fragments arranged by size: 2007 1823 877 776 376 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2627 10 Size: 54 1425 343 795 3426 Fragments arranged by size: 3426 1425 795 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 2477 2621 2840 4001 4360 4519 Size: 648 1134 177 144 219 1161 359 159 Cuts at: 4519 518 Size: 2042 Fragments arranged by size: 2042 1161 1134 648 359 219 177 159 144 MspA1I CmG'CkG Cuts at: 1606 2490 3224 3459 4136 5284 5529 1606 Size: 884 734 235 677 1148 245 2120 Fragments arranged by size: 2120 1148 884 734 677 245 235 NcoI C'CATG_G Cuts at: 513 1065 2028 513 Size: 552 963 4528 Fragments arranged by size: 4528 963 552 NdeI CA'TA_TG Cuts at: 387 2584 387 Size: 2197 3846 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 3185 3401 4946 1132 Size: 97 1956 216 1545 2229 Fragments arranged by size: 2229 1956 1545 216 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6043 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6043 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2283 3202 2283 Size: 919 5124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PstI C_TGCA'G Cuts at: 830 3395 830 Size: 2565 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2490 3224 2490 Size: 734 5309 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SfcI C'TryA_G Cuts at: 826 1045 2656 3046 3391 4526 5207 5398 826 Size: 219 1611 390 345 1135 681 191 1471 Fragments arranged by size: 1611 1471 1135 681 390 345 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 3370 1872 Size: 1498 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4147 5048 5310 820 Size: 197 926 2204 901 262 1553 Fragments arranged by size: 2204 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6043 StyI C'CwwG_G Cuts at: 513 1065 2028 2172 2199 2415 2694 3150 3240 Size: 552 963 144 27 216 279 456 90 Cuts at: 3240 3482 513 Size: 242 3074 Fragments arranged by size: 3074 963 552 456 279 242 216 144 90 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2290 2828 2952 3256 3351 4070 4087 Size: 1255 710 538 124 304 95 719 17 Cuts at: 4087 4240 4425 4870 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 719 710 538 445 304 185 153 124 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2458 4289 6001 Size: 274 80 33 51 1443 481 1831 1712 Cuts at: 6001 96 Size: 138 Fragments arranged by size: 1831 1712 1443 481 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2153 2185 6003 6035 2153 Size: 32 3818 32 2161 Fragments arranged by size: 3818 2161 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2452 2824 1316 Size: 1136 372 4535 Fragments arranged by size: 4535 1136 372 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 3368 1870 Size: 1498 4545 XmnI GAAnn'nnTTC Cuts at: 4172 4172 Size: 6043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3292 3851 276 Size: 53 83 186 936 1758 559 2468 Fragments arranged by size: 2468 1758 936 559 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI