Restriction map for
pFN21AB9867
(Linear) (Six-base) MAPSORT of: KIBB9867.seq Check: 5030 from: 1 to: 1338
KIBB9867.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 167 1338
Size: 167 1171
AatII G_ACGT'C
Cuts at: 0 1289 1338
Size: 1289 49
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1338
Size: 832 506
AflIII A'CryG_T
Cuts at: 0 941 1338
Size: 941 397
AlwNI CAG_nnn'CTG
Cuts at: 0 250 1338
Size: 250 1088
BanII G_rGCy'C
Cuts at: 0 1189 1338
Size: 1189 149
BbsI GAAGACnn'nnnn_
Cuts at: 0 820 1338
Size: 820 518
BbvCI CC'TCA_GC
Cuts at: 0 568 1338
Size: 568 770
BciVI GTATCCnnnnn_n'
Cuts at: 0 79 1338
Size: 79 1259
BclI T'GATC_A
Cuts at: 0 347 428 827 1338
Size: 347 81 399 511
Fragments arranged by size:
511 399 347 81
BglII A'GATC_T
Cuts at: 0 207 1338
Size: 207 1131
BlpI GC'TnA_GC
Cuts at: 0 481 1338
Size: 481 857
BmgBI CAC'GTC
Cuts at: 0 942 1338
Size: 942 396
BmrI ACTGGGnnnn_n'
Cuts at: 0 44 466 1338
Size: 44 422 872
Fragments arranged by size:
872 422 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 546 1315 1338
Size: 360 186 769 23
Fragments arranged by size:
769 360 186 23
Bpu10I CC'TnA_GC
Cuts at: 0 568 1338
Size: 568 770
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 1338
Size: 535 803
BsaHI Gr'CG_yC
Cuts at: 0 1286 1338
Size: 1286 52
BsaWI w'CCGG_w
Cuts at: 0 386 765 1338
Size: 386 379 573
Fragments arranged by size:
573 386 379
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 574 1231 1261 1338
Size: 544 30 657 30 77
Fragments arranged by size:
657 544 77 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1179 1225 1338
Size: 1179 46 113
Fragments arranged by size:
1179 113 46
BseYI C'CCAG_C
Cuts at: 0 1251 1338
Size: 1251 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1338
Size: 496 842
BsiHKAI G_wGCw'C
Cuts at: 0 319 1338
Size: 319 1019
BsmBI CGTCTCn'nnnn_
Cuts at: 0 898 1069 1293 1338
Size: 898 171 224 45
Fragments arranged by size:
898 224 171 45
Bsp1286I G_dGCh'C
Cuts at: 0 319 1189 1338
Size: 319 870 149
Fragments arranged by size:
870 319 149
BspHI T'CATG_A
Cuts at: 0 626 1122 1338
Size: 626 496 216
Fragments arranged by size:
626 496 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 1338
Size: 167 1171
BsrDI GCAATG_nn'
Cuts at: 0 680 1338
Size: 680 658
BssSI C'ACGA_G
Cuts at: 0 1130 1338
Size: 1130 208
BstEII G'GTnAC_C
Cuts at: 0 171 1338
Size: 171 1167
BstXI CCAn_nnnn'nTGG
Cuts at: 0 114 534 1236 1338
Size: 114 420 702 102
Fragments arranged by size:
702 420 114 102
BstYI r'GATC_y
Cuts at: 0 207 647 1301 1338
Size: 207 440 654 37
Fragments arranged by size:
654 440 207 37
BtgI C'CryG_G
Cuts at: 0 23 1338
Size: 23 1315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1120 1209 1338
Size: 1120 89 129
Fragments arranged by size:
1120 129 89
BtsI GCAGTG_nn'
Cuts at: 0 74 251 320 1338
Size: 74 177 69 1018
Fragments arranged by size:
1018 177 74 69
DrdI GACnn_nn'nnGTC
Cuts at: 0 1285 1338
Size: 1285 53
EaeI y'GGCC_r
Cuts at: 0 218 620 1338
Size: 218 402 718
Fragments arranged by size:
718 402 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 546 832 1315 1338
Size: 360 186 286 483 23
Fragments arranged by size:
483 360 286 186 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 642 693 1204 1338
Size: 642 51 511 134
Fragments arranged by size:
642 511 134 51
EcoO109I rG'GnC_Cy
Cuts at: 0 278 1197 1338
Size: 278 919 141
Fragments arranged by size:
919 278 141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 435 804 1338
Size: 435 369 534
Fragments arranged by size:
534 435 369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 1231 1263 1338
Size: 1124 32 75 32 75
Fragments arranged by size:
1124 75 75 32 32
HindIII A'AGCT_T
Cuts at: 0 603 1338
Size: 603 735
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 953 1329 1338
Size: 177 776 376 9
Fragments arranged by size:
776 376 177 9
MscI TGG'CCA
Cuts at: 0 622 1338
Size: 622 716
MslI CAynn'nnrTG
Cuts at: 0 295 472 616 835 1338
Size: 295 177 144 219 503
Fragments arranged by size:
503 295 219 177 144
MspA1I CmG'CkG
Cuts at: 0 485 1219 1338
Size: 485 734 119
Fragments arranged by size:
734 485 119
NcoI C'CATG_G
Cuts at: 0 23 1338
Size: 23 1315
NdeI CA'TA_TG
Cuts at: 0 579 1338
Size: 579 759
NspI r_CATG'y
Cuts at: 0 1180 1338
Size: 1180 158
PpuMI rG'GwC_Cy
Cuts at: 0 278 1197 1338
Size: 278 919 141
Fragments arranged by size:
919 278 141
PvuII CAG'CTG
Cuts at: 0 485 1219 1338
Size: 485 734 119
Fragments arranged by size:
734 485 119
SfcI C'TryA_G
Cuts at: 0 651 1041 1338
Size: 651 390 297
Fragments arranged by size:
651 390 297
StyI C'CwwG_G
Cuts at: 0 23 167 194 410 689 1145 1235 1338
Size: 23 144 27 216 279 456 90 103
Fragments arranged by size:
456 279 216 144 103 90 27 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 285 823 947 1251 1338
Size: 285 538 124 304 87
Fragments arranged by size:
538 304 285 124 87
TatI w'GTAC_w
Cuts at: 0 453 1338
Size: 453 885
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 1338
Size: 148 32 1158
Fragments arranged by size:
1158 148 32
Tth111I GACn'n_nGTC
Cuts at: 0 447 819 1338
Size: 447 372 519
Fragments arranged by size:
519 447 372
ZraI GAC'GTC
Cuts at: 0 1287 1338
Size: 1287 51
Enzymes that do cut:
AarI AatII AcuI AflIII AlwNI BanII BbsI BbvCI
BciVI BclI BglII BlpI BmgBI BmrI BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BssSI BstEII BstXI
BstYI BtgI BtgZI BtsI DrdI EaeI Eco57MI EcoNI
EcoO109I FalI Hin4I HindIII MmeI MscI MslI MspA1I
NcoI NdeI NspI PpuMI PvuII SfcI StyI TaqII
TatI TstI Tth111I ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BfrBI
BglI Bme1580I BmtI BplI BpuEI BsaAI BsaBI BsiEI
BsiWI BsmI BspEI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI
EarI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XcmI XhoI XmaI XmnI