(Circular) (Six-base) MAPSORT of: pFN21AA0018.seq Check: 5454 from: 1 to: 6253 pFN21AA0018 6253 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2224 2224 Size: 6253 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4063 278 Size: 53 83 186 936 2527 2468 Fragments arranged by size: 2527 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3225 3596 1937 Size: 1288 371 4594 Fragments arranged by size: 4594 1288 371 Acc65I G'GTAC_C Cuts at: 3574 3574 Size: 6253 AclI AA'CG_TT Cuts at: 1563 4380 4753 1563 Size: 2817 373 3063 Fragments arranged by size: 3063 2817 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2960 3122 4316 5700 5968 1632 Size: 452 876 162 1194 1384 268 1917 Fragments arranged by size: 1917 1384 1194 876 452 268 162 AfeI AGC'GCT Cuts at: 2096 2191 2096 Size: 95 6158 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6056 AflIII A'CryG_T Cuts at: 1203 2311 5152 6129 1203 Size: 1108 2841 977 1327 Fragments arranged by size: 2841 1327 1108 977 AgeI A'CCGG_T Cuts at: 5084 5084 Size: 6253 AhdI GACnn_n'nnGTC Cuts at: 4982 4982 Size: 6253 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2618 1184 Size: 1434 4819 AlwNI CAG_nnn'CTG Cuts at: 3196 3360 3412 3996 5568 3196 Size: 164 52 584 1572 3881 Fragments arranged by size: 3881 1572 584 164 52 ApaLI G'TGCA_C Cuts at: 2275 3275 4310 5466 2275 Size: 1000 1035 1156 3062 Fragments arranged by size: 3062 1156 1035 1000 ApoI r'AATT_y Cuts at: 1493 3562 3788 3940 6049 1493 Size: 2069 226 152 2109 1697 Fragments arranged by size: 2109 2069 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4807 160 Size: 872 3775 1606 Fragments arranged by size: 3775 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 AvaI C'yCGr_G Cuts at: 1870 1943 3515 3578 1870 Size: 73 1572 63 4545 Fragments arranged by size: 4545 1572 73 63 BamHI G'GATC_C Cuts at: 3583 3950 3583 Size: 367 5886 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2283 3079 3169 3574 5029 Size: 325 217 627 496 796 90 405 1455 Cuts at: 5029 618 Size: 1842 Fragments arranged by size: 1842 1455 796 627 496 405 325 217 90 BanII G_rGCy'C Cuts at: 1391 2013 2409 2874 3021 3303 3572 1391 Size: 622 396 465 147 282 269 4072 Fragments arranged by size: 4072 622 465 396 282 269 147 BbeI G_GCGC'C Cuts at: 2287 2287 Size: 6253 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2336 2948 928 Size: 971 437 612 4233 Fragments arranged by size: 4233 971 612 437 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2503 2537 4444 4478 1903 Size: 34 566 34 1907 34 3678 Fragments arranged by size: 3678 1907 566 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4145 5355 4145 Size: 1210 5043 BclI T'GATC_A Cuts at: 1548 2349 2505 1548 Size: 801 156 5296 Fragments arranged by size: 5296 801 156 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3267 4864 136 Size: 107 122 71 631 605 1595 1597 1525 Fragments arranged by size: 1597 1595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3090 3531 6248 3090 Size: 441 2717 3095 Fragments arranged by size: 3095 2717 441 BlpI GC'TnA_GC Cuts at: 2183 3670 2183 Size: 1487 4766 Bme1580I G_kGCm'C Cuts at: 1790 2279 2516 3174 3279 4314 5470 1790 Size: 489 237 658 105 1035 1156 2573 Fragments arranged by size: 2573 1156 1035 658 489 237 105 BmgBI CAC'GTC Cuts at: 1318 3223 1318 Size: 1905 4348 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3984 4942 442 Size: 416 53 83 399 24 2567 958 1753 Fragments arranged by size: 2567 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2768 2981 3005 3395 4913 1239 Size: 854 675 213 24 390 1518 2579 Fragments arranged by size: 2579 1518 854 675 390 213 24 Bpu10I CC'TnA_GC Cuts at: 2475 2475 Size: 6253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2995 4378 5243 5541 2995 Size: 1383 865 298 3707 Fragments arranged by size: 3707 1383 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3113 4916 882 Size: 617 1614 1803 2219 Fragments arranged by size: 2219 1803 1614 617 BsaAI yAC'GTr Cuts at: 493 1204 2158 2312 3151 4044 493 Size: 711 954 154 839 893 2702 Fragments arranged by size: 2702 954 893 839 711 154 BsaBI GATnn'nnATC Cuts at: 2693 6247 2693 Size: 3554 2699 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2284 3507 4060 4442 Size: 53 83 186 936 751 1223 553 382 Cuts at: 4442 5077 275 Size: 635 1451 Fragments arranged by size: 1451 1223 936 751 635 553 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4686 5084 5358 5505 5916 4686 Size: 398 274 147 411 5023 Fragments arranged by size: 5023 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6028 Fragments arranged by size: 6028 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3455 1186 Size: 2269 3984 BseYI C'CCAG_C Cuts at: 2097 2665 2781 2873 3257 3493 5456 5952 2097 Size: 568 116 92 384 236 1963 496 2398 Fragments arranged by size: 2398 1963 568 496 384 236 116 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2548 3176 3182 1163 Size: 573 450 362 628 6 4234 Fragments arranged by size: 4234 628 573 450 362 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3703 4464 5492 1540 Size: 275 186 1702 761 1028 2301 Fragments arranged by size: 2301 1702 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2279 3021 3279 3303 4314 4399 5470 2085 Size: 194 742 258 24 1011 85 1071 2868 Fragments arranged by size: 2868 1071 1011 742 258 194 85 24 BsmI GAATG_Cn' Cuts at: 3764 3857 3764 Size: 93 6160 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2085 2279 2409 2516 2874 3021 Size: 399 223 72 194 130 107 358 147 Cuts at: 3021 3174 3279 3303 3572 4314 4399 5470 1391 Size: 153 105 24 269 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 358 269 223 194 153 147 130 107 105 85 72 24 BspEI T'CCGG_A Cuts at: 5916 5916 Size: 6253 BspHI T'CATG_A Cuts at: 2346 2853 4142 5872 2346 Size: 507 1289 1730 2727 Fragments arranged by size: 2727 1730 1289 507 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2224 3608 844 Size: 1380 1384 3489 Fragments arranged by size: 3489 1384 1380 BsrBI CCG'CTC Cuts at: 1394 1823 2128 4140 1394 Size: 429 305 2012 3507 Fragments arranged by size: 3507 2012 429 305 BsrDI GCAATG_nn' Cuts at: 65 1248 4748 4922 65 Size: 1183 3500 174 1396 Fragments arranged by size: 3500 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4897 5084 1700 Size: 110 3087 187 2869 Fragments arranged by size: 3087 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6253 BssHII G'CGCG_C Cuts at: 1925 2034 2161 1925 Size: 109 127 6017 Fragments arranged by size: 6017 127 109 BssSI C'ACGA_G Cuts at: 2204 2774 4313 5325 2204 Size: 570 1539 1012 3132 Fragments arranged by size: 3132 1539 1012 570 BstAPI GCAn_nnn'nTGC Cuts at: 794 2215 3163 3196 794 Size: 1421 948 33 3851 Fragments arranged by size: 3851 1421 948 33 BstBI TT'CG_AA Cuts at: 5057 5057 Size: 6253 BstEII G'GTnAC_C Cuts at: 1184 2413 1184 Size: 1229 5024 BstYI r'GATC_y Cuts at: 1972 2688 3090 3531 3583 3950 4335 4352 5804 Size: 716 402 441 52 367 385 17 1452 Cuts at: 5804 5890 5902 6248 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 716 441 402 385 367 346 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2418 2909 513 Size: 552 939 414 491 3857 Fragments arranged by size: 3857 939 552 491 414 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2620 487 Size: 683 56 1394 4120 Fragments arranged by size: 4120 1394 683 56 BtsI GCAGTG_nn' Cuts at: 2565 3769 4563 4583 2565 Size: 1204 794 20 4235 Fragments arranged by size: 4235 1204 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6253 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6218 DraI TTT'AAA Cuts at: 3557 3557 Size: 6253 DraIII CAC_nnn'GTG Cuts at: 2312 2803 3151 3154 2312 Size: 491 348 3 5411 Fragments arranged by size: 5411 491 348 3 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3221 5260 809 Size: 507 1905 2039 1802 Fragments arranged by size: 2039 1905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2679 3700 Size: 54 1425 50 275 18 195 654 1021 Cuts at: 3700 4589 6218 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1021 889 654 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3700 1537 Size: 275 1888 4090 Fragments arranged by size: 4090 1888 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 2120 4183 1353 Size: 695 72 2063 3423 Fragments arranged by size: 3423 2063 695 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4840 5214 5360 387 Size: 1064 138 3251 374 146 1280 Fragments arranged by size: 3251 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3019 3301 3019 Size: 282 5971 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2093 2768 2960 2981 3005 3122 Size: 393 452 9 675 192 21 24 117 Cuts at: 3122 3395 4316 4913 5700 5968 1239 Size: 273 921 597 787 268 1524 Fragments arranged by size: 1524 921 787 675 597 452 393 273 268 192 117 24 21 9 EcoNI CCTnn'n_nnAGG Cuts at: 2828 3188 3364 2828 Size: 360 176 5717 Fragments arranged by size: 5717 360 176 EcoO109I rG'GnC_Cy Cuts at: 2743 2956 3193 3357 2743 Size: 213 237 164 5639 Fragments arranged by size: 5639 237 213 164 EcoRI G'AATT_C Cuts at: 3562 3940 3562 Size: 378 5875 EcoRV GAT'ATC Cuts at: 2146 2146 Size: 6253 FspI TGC'GCA Cuts at: 3128 4759 3128 Size: 1631 4622 HaeII r_GCGC'y Cuts at: 1678 2098 2193 2287 5400 1678 Size: 420 95 94 3113 2531 Fragments arranged by size: 3113 2531 420 95 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2516 2548 3067 3099 4894 Size: 32 135 32 944 32 519 32 1795 Cuts at: 4894 4926 4968 5000 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1795 944 519 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3597 3843 669 Size: 988 281 1659 246 3079 Fragments arranged by size: 3079 1659 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6253 HpaI GTT'AAC Cuts at: 3843 3843 Size: 6253 KasI G'GCGC_C Cuts at: 2283 2283 Size: 6253 KpnI G_GTAC'C Cuts at: 3578 3578 Size: 6253 MfeI C'AATT_G Cuts at: 3852 3852 Size: 6253 MluI A'CGCG_T Cuts at: 6129 6129 Size: 6253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2578 5367 5551 1305 Size: 1273 2789 184 2007 Fragments arranged by size: 2789 2007 1273 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4431 Fragments arranged by size: 4431 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3167 4211 4570 4729 518 Size: 648 2001 1044 359 159 2042 Fragments arranged by size: 2042 2001 1044 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2684 2785 3373 3669 4346 5494 5739 1606 Size: 1078 101 588 296 677 1148 245 2120 Fragments arranged by size: 2120 1148 1078 677 588 296 245 101 NarI GG'CG_CC Cuts at: 2284 2284 Size: 6253 NcoI C'CATG_G Cuts at: 513 1065 2004 2418 513 Size: 552 939 414 4348 Fragments arranged by size: 4348 939 552 414 NdeI CA'TA_TG Cuts at: 387 3322 387 Size: 2935 3318 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6253 NotI GC'GGCC_GC Cuts at: 3700 3700 Size: 6253 NspI r_CATG'y Cuts at: 1132 1229 2927 3166 3611 5156 1132 Size: 97 1698 239 445 1545 2229 Fragments arranged by size: 2229 1698 1545 445 239 97 PasI CC'CwG_GG Cuts at: 1348 3112 1348 Size: 1764 4489 PciI A'CATG_T Cuts at: 5152 5152 Size: 6253 PflMI CCAn_nnn'nTGG Cuts at: 1108 2454 2803 1108 Size: 1346 349 4558 Fragments arranged by size: 4558 1346 349 PfoI T'CCnGG_A Cuts at: 3021 3021 Size: 6253 PmeI GTTT'AAAC Cuts at: 3557 3557 Size: 6253 PmlI CAC'GTG Cuts at: 3151 3151 Size: 6253 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4322 4354 5835 5867 4322 Size: 32 1481 32 4708 Fragments arranged by size: 4708 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3357 3357 Size: 6253 PshAI GACnn'nnGTC Cuts at: 1613 2849 1613 Size: 1236 5017 PsiI TTA'TAA Cuts at: 3823 3823 Size: 6253 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2534 2566 2534 Size: 32 6221 PstI C_TGCA'G Cuts at: 830 3189 3605 830 Size: 2359 416 3478 Fragments arranged by size: 3478 2359 416 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6253 PvuII CAG'CTG Cuts at: 3373 3373 Size: 6253 SacI G_AGCT'C Cuts at: 3021 3303 3021 Size: 282 5971 SalI G'TCGA_C Cuts at: 1936 3595 1936 Size: 1659 4594 SapI GCTCTTCn'nnn_ Cuts at: 2048 2048 Size: 6253 SbfI CC_TGCA'GG Cuts at: 3605 3605 Size: 6253 ScaI AGT'ACT Cuts at: 4501 6213 4501 Size: 1712 4541 SfcI C'TryA_G Cuts at: 826 1045 3185 3601 4736 5417 5608 826 Size: 219 2140 416 1135 681 191 1471 Fragments arranged by size: 2140 1471 1135 681 416 219 191 SfoI GGC'GCC Cuts at: 2285 2285 Size: 6253 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6253 SmaI CCC'GGG Cuts at: 1872 3580 1872 Size: 1708 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2974 4357 5258 5520 820 Size: 197 926 1031 1383 901 262 1553 Fragments arranged by size: 1553 1383 1031 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4044 493 Size: 3551 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6253 SphI G_CATG'C Cuts at: 1132 3611 1132 Size: 2479 3774 SspI AAT'ATT Cuts at: 5 52 4177 5 Size: 47 4125 2081 Fragments arranged by size: 4125 2081 47 StuI AGG'CCT Cuts at: 1517 3270 1517 Size: 1753 4500 StyI C'CwwG_G Cuts at: 513 1065 2004 2409 2418 3692 513 Size: 552 939 405 9 1274 3074 Fragments arranged by size: 3074 1274 939 552 405 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2044 2089 2158 2786 4280 4297 4450 Size: 1255 464 45 69 628 1494 17 153 Cuts at: 4450 4635 5080 325 Size: 185 445 1498 Fragments arranged by size: 1498 1494 1255 628 464 445 185 153 69 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2964 4499 Size: 274 80 33 51 1443 345 642 1535 Cuts at: 4499 6211 96 Size: 1712 138 Fragments arranged by size: 1712 1535 1443 642 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6213 6245 6213 Size: 32 6221 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6253 XbaI T'CTAG_A Cuts at: 3589 3589 Size: 6253 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6253 XmaI C'CCGG_G Cuts at: 1870 3578 1870 Size: 1708 4545 XmnI GAAnn'nnTTC Cuts at: 2798 4382 2798 Size: 1584 4669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4061 276 Size: 53 83 186 936 2527 2468 Fragments arranged by size: 2527 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI