(Circular) (Six-base) MAPSORT of: pFN21AB9855.seq Check: 5517 from: 1 to: 5875 pFN21AB9855 5875 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2647 2647 Size: 5875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 2079 3196 2079 Size: 1117 4758 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2536 3938 5322 5590 1632 Size: 904 1402 1384 268 1917 Fragments arranged by size: 1917 1402 1384 904 268 AfeI AGC'GCT Cuts at: 2945 2945 Size: 5875 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 2178 2605 4774 5751 1203 Size: 975 427 2169 977 1327 Fragments arranged by size: 2169 1327 977 975 427 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 2192 1166 Size: 1026 4849 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2270 1184 Size: 1086 4789 AlwNI CAG_nnn'CTG Cuts at: 3618 5190 3618 Size: 1572 4303 ApaI G_GGCC'C Cuts at: 2104 3105 2104 Size: 1001 4874 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2681 2846 3200 1870 Size: 73 87 651 165 354 4545 Fragments arranged by size: 4545 651 354 165 87 73 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2079 2695 2815 3196 4651 Size: 325 217 627 292 616 120 381 1455 Cuts at: 4651 618 Size: 1842 Fragments arranged by size: 1842 1455 627 616 381 325 292 217 120 BanII G_rGCy'C Cuts at: 1391 2076 2104 2331 2475 3105 3194 1391 Size: 685 28 227 144 630 89 4072 Fragments arranged by size: 4072 685 630 227 144 89 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2519 928 Size: 971 620 4284 Fragments arranged by size: 4284 971 620 BbvCI CC'TCA_GC Cuts at: 2964 3091 2964 Size: 127 5748 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2303 2337 4066 4100 1903 Size: 34 50 34 282 34 1729 34 3678 Fragments arranged by size: 3678 1729 282 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 2478 2631 2769 1548 Size: 930 153 138 4654 Fragments arranged by size: 4654 930 153 138 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2685 4486 136 Size: 107 122 71 631 605 1013 1801 1525 Fragments arranged by size: 1801 1525 1013 631 605 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 2325 3292 2325 Size: 967 4908 Bme1580I G_kGCm'C Cuts at: 1790 2089 2104 2321 2432 2700 2955 3024 3105 Size: 299 15 217 111 268 255 69 81 Cuts at: 3105 3126 3936 5092 1790 Size: 21 810 1156 2573 Fragments arranged by size: 2573 1156 810 299 268 255 217 111 81 69 21 15 BmgBI CAC'GTC Cuts at: 1318 2179 1318 Size: 861 5014 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3144 4535 1239 Size: 1905 1391 2579 Fragments arranged by size: 2579 1905 1391 Bpu10I CC'TnA_GC Cuts at: 2964 3091 2964 Size: 127 5748 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2186 4000 4865 5163 2186 Size: 1814 865 298 2898 Fragments arranged by size: 2898 1814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2534 4538 882 Size: 617 1035 2004 2219 Fragments arranged by size: 2219 2004 1035 617 BsaAI yAC'GTr Cuts at: 493 1204 2041 2606 3666 493 Size: 711 837 565 1060 2702 Fragments arranged by size: 2702 1060 837 711 565 BsaBI GATnn'nnATC Cuts at: 2450 5869 2450 Size: 3419 2456 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5650 Fragments arranged by size: 5650 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 2981 1186 Size: 1252 543 4080 Fragments arranged by size: 4080 1252 543 BseYI C'CCAG_C Cuts at: 2309 2819 5078 5574 2309 Size: 510 2259 496 2610 Fragments arranged by size: 2610 2259 510 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2266 2477 2813 3019 1163 Size: 573 530 211 336 206 4019 Fragments arranged by size: 4019 573 530 336 211 206 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2587 3325 4086 5114 1540 Size: 275 186 586 738 761 1028 2301 Fragments arranged by size: 2301 1028 761 738 586 275 186 BsiHKAI G_wGCw'C Cuts at: 3936 4021 5092 3936 Size: 85 1071 4719 Fragments arranged by size: 4719 1071 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2089 2104 2321 2331 2432 2475 Size: 399 286 13 15 217 10 101 43 Cuts at: 2475 2700 2955 3024 3105 3126 3194 3936 4021 Size: 225 255 69 81 21 68 742 85 Cuts at: 4021 5092 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 286 255 225 217 101 85 81 69 68 43 21 15 13 10 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 2475 2508 3764 5494 2475 Size: 33 1256 1730 2856 Fragments arranged by size: 2856 1730 1256 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2647 2835 3230 844 Size: 1803 188 395 3489 Fragments arranged by size: 3489 1803 395 188 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2216 4370 4544 65 Size: 1183 968 2154 174 1396 Fragments arranged by size: 2154 1396 1183 968 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4519 4706 1700 Size: 110 2709 187 2869 Fragments arranged by size: 2869 2709 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 2297 2612 1184 Size: 1113 315 4447 Fragments arranged by size: 4447 1113 315 BstXI CCAn_nnnn'nTGG Cuts at: 2194 2194 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2928 3205 3572 3957 3974 5426 5512 5524 Size: 956 277 367 385 17 1452 86 12 Cuts at: 5524 5870 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 956 385 367 346 277 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2265 2293 2581 2721 2883 3097 3126 Size: 552 1200 28 288 140 162 214 29 Cuts at: 3126 513 Size: 3262 Fragments arranged by size: 3262 1200 552 288 214 162 140 29 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2266 487 Size: 683 56 1040 4096 Fragments arranged by size: 4096 1040 683 56 BtsI GCAGTG_nn' Cuts at: 2319 3391 4185 4205 2319 Size: 1072 794 20 3989 Fragments arranged by size: 3989 1072 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2199 2234 529 Size: 35 1635 35 4170 Fragments arranged by size: 4170 1635 35 35 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DraIII CAC_nnn'GTG Cuts at: 2710 2710 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2207 2436 2584 Size: 54 1425 50 275 18 377 229 148 Cuts at: 2584 2919 3129 3322 4211 5840 8 Size: 335 210 193 889 1629 43 Fragments arranged by size: 1629 1425 889 377 335 275 229 210 193 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2584 3322 1537 Size: 275 772 738 4090 Fragments arranged by size: 4090 772 738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 3805 1353 Size: 911 1541 3423 Fragments arranged by size: 3423 1541 911 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2536 3144 3938 4535 5322 5590 1239 Size: 393 904 608 794 597 787 268 1524 Fragments arranged by size: 1524 904 794 787 608 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2705 2057 Size: 648 5227 EcoO109I rG'GnC_Cy Cuts at: 2062 2100 2842 3102 2062 Size: 38 742 260 4835 Fragments arranged by size: 4835 742 260 38 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 FspI TGC'GCA Cuts at: 3110 4381 3110 Size: 1271 4604 HaeII r_GCGC'y Cuts at: 1678 2643 2947 5022 1678 Size: 965 304 2075 2531 Fragments arranged by size: 2531 2075 965 304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3155 3187 4516 4548 4590 Size: 32 135 32 1583 32 1329 32 42 Cuts at: 4590 4622 1373 Size: 32 2626 Fragments arranged by size: 2626 1583 1329 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 2083 3200 2083 Size: 1117 4758 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4989 5173 1305 Size: 3684 184 2007 Fragments arranged by size: 3684 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2209 2438 3131 10 Size: 54 1425 343 377 229 693 2754 Fragments arranged by size: 2754 1425 693 377 343 229 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2507 2651 2657 2720 3017 3833 Size: 648 1026 315 144 6 63 297 816 Cuts at: 3833 4192 4351 518 Size: 359 159 2042 Fragments arranged by size: 2042 1026 816 648 359 315 297 159 144 63 6 MspA1I CmG'CkG Cuts at: 1606 2095 2250 2767 3099 3291 3968 5116 5361 Size: 489 155 517 332 192 677 1148 245 Cuts at: 5361 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 517 489 332 245 192 155 NcoI C'CATG_G Cuts at: 513 1065 2265 2721 3126 513 Size: 552 1200 456 405 3262 Fragments arranged by size: 3262 1200 552 456 405 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 2564 2656 3233 4778 1132 Size: 97 1335 92 577 1545 2229 Fragments arranged by size: 2229 1545 1335 577 97 92 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 2271 2710 1108 Size: 1163 439 4273 Fragments arranged by size: 4273 1163 439 PfoI T'CCnGG_A Cuts at: 2151 2862 2151 Size: 711 5164 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2842 2062 Size: 780 5095 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspOMI G'GGCC_C Cuts at: 2100 3101 2100 Size: 1001 4874 PstI C_TGCA'G Cuts at: 830 2175 3227 830 Size: 1345 1052 3478 Fragments arranged by size: 3478 1345 1052 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2250 2250 Size: 5875 SacII CC_GC'GG Cuts at: 3100 3100 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SapI GCTCTTCn'nnn_ Cuts at: 2264 2264 Size: 5875 SbfI CC_TGCA'GG Cuts at: 2175 3227 2175 Size: 1052 4823 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SexAI A'CCwGG_T Cuts at: 2706 2706 Size: 5875 SfcI C'TryA_G Cuts at: 826 1045 2171 3223 4358 5039 5230 826 Size: 219 1126 1052 1135 681 191 1471 Fragments arranged by size: 1471 1135 1126 1052 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2201 2846 3979 4880 5142 820 Size: 197 926 258 645 1133 901 262 1553 Fragments arranged by size: 1553 1133 926 901 645 262 258 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 2564 3233 1132 Size: 1432 669 3774 Fragments arranged by size: 3774 1432 669 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 2265 2721 3126 3132 3314 513 Size: 552 1200 456 405 6 182 3074 Fragments arranged by size: 3074 1200 552 456 405 182 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2600 2726 2833 2912 3902 3919 4072 Size: 1255 1020 126 107 79 990 17 153 Cuts at: 4072 4257 4702 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1020 990 445 185 153 126 107 79 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4121 5833 96 Size: 274 80 33 51 1443 2144 1712 138 Fragments arranged by size: 2144 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2189 2221 2424 2456 5835 5867 2189 Size: 32 203 32 3379 32 2197 Fragments arranged by size: 3379 2197 203 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2393 2393 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 2846 1943 Size: 903 4972 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI SwaI