Restriction map for
pFN21AA0001
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 652 CTTAAG C^TTAAG
AlwI 231 GATCC GGATC(4/5)
Asp700I 828 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 295 GTGCTC G[AT]GC[AT]^C
BalI 575 TGGCCA TGG^CCA
BanII 337 GGGCTC G[GA]GC[CT]^C
BbsI 94 GTCTTC GAAGAC(2/6)
BfrI 652 CTTAAG C^TTAAG
BpmI 26 CTCCAG CTGGAG(16/14)
BpuAI 94 GTCTTC GAAGAC(2/6)
BsaAI 145 TACGTG [CT]AC^GT[GA]
BsaI 285 GGTCTC GGTCTC(1/5)
BseRI 448 CTCCTC GAGGAG(10/8)
BsgI 279 GTGCAG GTGCAG(16/14)
BsiHKAI 295 GTGCTC G[AT]GC[AT]^C
BsiYI 578 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 578 CCATCTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 286 GTCTC GTCTC(1/5)
BspLU11I 311 ACATGT A^CATGT
BsrDI 99 CATTGC GCAATG(2/0)
BssKI 257 CCTGG ^CC[ACGT]GG
BstNI 257 CCTGG CC^[AT]GG
BstYI 230 AGATCC [GA]^GATC[CT]
Cac8I 896 GCCAGC GC[ACGT]^[ACGT]GC
EaeI 575 TGGCCA [CT]^GGCC[GA]
EarI 298 CTCTTC CTCTTC(1/4)
EcoRII 257 CCTGG ^CC[AT]GG
HincII 307 GTCAAC GT[CT]^[GA]AC
HindII 307 GTCAAC GT[CT]^[GA]AC
Ksp632I 298 CTCTTC CTCTTC(1/4)
MflI 230 AGATCC [GA]^GATC[CT]
MluNI 575 TGGCCA TGG^CCA
MscI 575 TGGCCA TGG^CCA
MvaI 257 CCTGG CC^[AT]GG
NspI 311 ACATGT [GA]CATG^[CT]
PflMI 578 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 297 GCTCTTC GCTCTTC(1/4)
ScrFI 257 CCTGG CC^[ACGT]GG
SspI 466 AATATT AAT^ATT
StuI 246 AGGCCT AGG^CCT
Tsp45I 239 GTGAC ^GT[GC]AC
Van91I 578 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 230 AGATCC [GA]^GATC[CT]
XmnI 828 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 696 CCGC CCGC(-3/-1)
819 GCGG
AcsI 666 GAATTC [GA]^AATT[CT]
832 GAATTC
AflIII 269 ACGTGT A^C[GA][CT]GT
311 ACATGT
ApoI 666 GAATTC [GA]^AATT[CT]
832 GAATTC
AvaII 253 GGTCC G^G[AT]CC
870 GGACC
BclI 2 TGATCA T^GATCA
56 TGATCA
BfaI 692 CTAG C^TAG
958 CTAG
Cfr13I 253 GGTCC G^G[ACGT]CC
870 GGACC
EcoRI 666 GAATTC G^AATTC
832 GAATTC
EcoT14I 234 CCTTGG C^C[AT][AT]GG
249 CCTTGG
FbaI 2 TGATCA T^GATCA
56 TGATCA
HaeIII 247 GGCC GG^CC
576 GGCC
HphI 238 GGTGA GGTGA(8/7)
476 TCACC
MaeI 692 CTAG C^TAG
958 CTAG
MslI 72 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
567 CATCTTCGTG
MspA1I 262 CAGCTG C[AC]G^C[GT]G
795 CAGCTG
NlaIII 312 CATG CATG^
444 CATG
NlaIV 253 GGTCCC GG[ACGT]^[ACGT]CC
870 GGACCC
PleI 241 GACTC GAGTC(4/5)
774 GAGTC
PvuII 262 CAGCTG CAG^CTG
795 CAGCTG
Sau96I 253 GGTCC G^G[ACGT]CC
870 GGACC
SmlI 178 CTCAAG C^T[CT][GA]AG
652 CTTAAG
StyI 234 CCTTGG C^C[AT][AT]GG
249 CCTTGG
TspRI 401 CAGTG CA[GC]TG[ACGT][ACGT]^
425 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BsmFI Bsp1286I BsrI BstF5I DdeI
DpnII Fnu4HI FokI HinfI ItaI
MaeII MaeIII MboI MboII MnlI
MseI NdeII RsaI Sau3AI SfaNI
TfiI Tru9I TseI Tsp509I