Restriction map for
pFN21ASDA0585
(Circular) (Six-base) MAPSORT of: pFN21ASDA0585.seq Check: 2709 from: 1 to: 5971
pFN21ASDA0585 5971 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3002 4034 5418 5686 1632
Size: 1370 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1370 1032 268
AfeI AGC'GCT
Cuts at: 2636 2636
Size: 5971
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 2607 3714 5286 2607
Size: 1107 1572 3292
Fragments arranged by size:
3292 1572 1107
ApaLI G'TGCA_C
Cuts at: 3134 4028 5184 3134
Size: 894 1156 3921
Fragments arranged by size:
3921 1156 894
ApoI r'AATT_y
Cuts at: 1493 2195 2774 2881 3280 3506 3658 5767 1493
Size: 702 579 107 399 226 152 2109 1697
Fragments arranged by size:
2109 1697 702 579 399 226 152 107
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2394 2685 2984 3296 1870
Size: 73 451 291 299 312 4545
Fragments arranged by size:
4545 451 312 299 291 73
BamHI G'GATC_C
Cuts at: 3113 3301 3668 3113
Size: 188 367 5416
Fragments arranged by size:
5416 367 188
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2476 2640 3292 4747 618
Size: 325 217 627 689 164 652 1455 1842
Fragments arranged by size:
1842 1455 689 652 627 325 217 164
BanII G_rGCy'C
Cuts at: 1391 2082 2138 3259 3290 1391
Size: 691 56 1121 31 4072
Fragments arranged by size:
4072 1121 691 56 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2472 928
Size: 971 573 4427
Fragments arranged by size:
4427 971 573
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 2132 3388 2132
Size: 1256 4715
Bme1580I G_kGCm'C
Cuts at: 1790 3138 4032 5188 1790
Size: 1348 894 1156 2573
Fragments arranged by size:
2573 1348 1156 894
BmgBI CAC'GTC
Cuts at: 1318 3159 1318
Size: 1841 4130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2583 3702 4660
Size: 416 53 83 399 24 1166 1119 958
Cuts at: 4660 442
Size: 1753
Fragments arranged by size:
1753 1166 1119 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 3238 2143
Size: 1095 4876
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 3034 3055 3061 3211 4631 1239
Size: 1064 731 21 6 150 1420 2579
Fragments arranged by size:
2579 1420 1064 731 150 21 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 4096 4961 5259 2066
Size: 2030 865 298 2778
Fragments arranged by size:
2778 2030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2929 4634 882
Size: 617 1430 1705 2219
Fragments arranged by size:
2219 1705 1430 617
BsaAI yAC'GTr
Cuts at: 493 1204 3268 3762 493
Size: 711 2064 494 2702
Fragments arranged by size:
2702 2064 711 494
BsaBI GATnn'nnATC
Cuts at: 2582 5965 2582
Size: 3383 2588
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2567 4404 4802 5076 5223 5634 2567
Size: 1837 398 274 147 411 2904
Fragments arranged by size:
2904 1837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2644 2674 2799 2829 3173
Size: 30 165 30 1241 30 125 30 344
Cuts at: 3173 3203 1178
Size: 30 3946
Fragments arranged by size:
3946 1241 344 165 125 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3058 3166 3214 1186
Size: 1872 108 48 3943
Fragments arranged by size:
3943 1872 108 48
BseYI C'CCAG_C
Cuts at: 2513 5174 5670 2513
Size: 2661 496 2814
Fragments arranged by size:
2814 2661 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3181 1163
Size: 573 1445 3953
Fragments arranged by size:
3953 1445 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2075 2145 3421 4182 5210 1540
Size: 275 186 74 70 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186 74 70
BsiHKAI G_wGCw'C
Cuts at: 2082 2393 2982 3138 4032 4117 5188 2082
Size: 311 589 156 894 85 1071 2865
Fragments arranged by size:
2865 1071 894 589 311 156 85
BsmI GAATG_Cn'
Cuts at: 2248 2617 3482 3575 2248
Size: 369 865 93 4644
Fragments arranged by size:
4644 865 369 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2682 2682
Size: 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2138 2393 2982 3138 3259 3290
Size: 399 292 56 255 589 156 121 31
Cuts at: 3290 4032 4117 5188 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 589 399 292 255 156
121 85 56 31
BspEI T'CCGG_A
Cuts at: 2567 5634 2567
Size: 3067 2904
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 844
Size: 2482 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 3858 1394
Size: 429 383 1652 3507
Fragments arranged by size:
3507 1652 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 4466 4640 65
Size: 1183 3218 174 1396
Fragments arranged by size:
3218 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4615 4802 1700
Size: 110 2805 187 2869
Fragments arranged by size:
2869 2805 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 2007 4775 2007
Size: 2768 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstXI CCAn_nnnn'nTGG
Cuts at: 2513 2832 2513
Size: 319 5652
BstYI r'GATC_y
Cuts at: 1972 3113 3301 3668 4053 4070 5522 5608 5620
Size: 1141 188 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1141 385 367 346 188 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2145 2229 513
Size: 552 1080 84 4255
Fragments arranged by size:
4255 1080 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3138 487
Size: 683 56 1912 3320
Fragments arranged by size:
3320 1912 683 56
BtsI GCAGTG_nn'
Cuts at: 3487 4281 4301 3487
Size: 794 20 5157
Fragments arranged by size:
5157 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3203 4978 809
Size: 507 1887 1775 1802
Fragments arranged by size:
1887 1802 1775 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2072 2142 2765
Size: 54 1425 50 275 18 242 70 623
Cuts at: 2765 3418 4307 5936 8
Size: 653 889 1629 43
Fragments arranged by size:
1629 1425 889 653 623 275 242 70
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2072 2142 3418 1537
Size: 275 260 70 1276 4090
Fragments arranged by size:
4090 1276 275 260 70
EarI CTCTTCn'nnn_
Cuts at: 1353 3064 3143 3901 1353
Size: 1711 79 758 3423
Fragments arranged by size:
3423 1711 758 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2165 4558 4932 5078 387
Size: 1064 138 576 2393 374 146 1280
Fragments arranged by size:
2393 1280 1064 576 374 146 138
EcoICRI GAG'CTC
Cuts at: 2080 2080
Size: 5971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 3002 3034 3055 3061 3211 4034
Size: 393 671 699 32 21 6 150 823
Cuts at: 4034 4631 5418 5686 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 823 787 699 671 597 393 268
150 32 21 6
EcoO109I rG'GnC_Cy
Cuts at: 2529 2529
Size: 5971
EcoRI G'AATT_C
Cuts at: 2774 3280 3658 2774
Size: 506 378 5087
Fragments arranged by size:
5087 506 378
FspI TGC'GCA
Cuts at: 2247 2265 4477 2247
Size: 18 2212 3741
Fragments arranged by size:
3741 2212 18
HaeII r_GCGC'y
Cuts at: 1678 2290 2638 5118 1678
Size: 612 348 2480 2531
Fragments arranged by size:
2531 2480 612 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2330 2362 2799
Size: 32 135 32 671 32 55 32 437
Cuts at: 2799 2831 3080 3092 3098 3112 3124 3130 3173
Size: 32 249 12 6 14 12 6 43
Cuts at: 3173 3205 4612 4644 4686 4718 1373
Size: 32 1407 32 42 32 2626
Fragments arranged by size:
2626 1407 671 437 249 135 55 43
42 32 32 32 32 32 32 32
32 14 12 12 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3013 3315 3561 669
Size: 988 281 1075 302 246 3079
Fragments arranged by size:
3079 1075 988 302 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2783 3066 3117 5085 5269 1305
Size: 1478 283 51 1968 184 2007
Fragments arranged by size:
2007 1968 1478 283 184 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2767 10
Size: 54 1425 343 935 3214
Fragments arranged by size:
3214 1425 935 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 3929 4288 4447 518
Size: 648 1512 1251 359 159 2042
Fragments arranged by size:
2042 1512 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 3387 4064 5212 5457 1606
Size: 576 1205 677 1148 245 2120
Fragments arranged by size:
2120 1205 1148 677 576 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5419
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2843 3329 4874 1132
Size: 97 1614 486 1545 2229
Fragments arranged by size:
2229 1614 1545 486 97
PasI CC'CwG_GG
Cuts at: 1348 2664 1348
Size: 1316 4655
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2607 1108
Size: 1499 4472
PfoI T'CCnGG_A
Cuts at: 3227 3227
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 3323 830
Size: 2493 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2182 2182
Size: 5971
SacI G_AGCT'C
Cuts at: 2082 2082
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SexAI A'CCwGG_T
Cuts at: 2723 2723
Size: 5971
SfcI C'TryA_G
Cuts at: 826 1045 3319 4454 5135 5326 826
Size: 219 2274 1135 681 191 1471
Fragments arranged by size:
2274 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2081 2394 4075 4976 5238 820
Size: 197 926 138 313 1681 901 262 1553
Fragments arranged by size:
1681 1553 926 901 313 262 197 138
SnaBI TAC'GTA
Cuts at: 493 3268 3762 493
Size: 2775 494 2702
Fragments arranged by size:
2775 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 3410 513
Size: 552 2345 3074
Fragments arranged by size:
3074 2345 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2891 3998 4015 4168 4353 4798 325
Size: 1255 1311 1107 17 153 185 445 1498
Fragments arranged by size:
1498 1311 1255 1107 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 4217 5929
Size: 274 80 33 51 1443 402 1838 1712
Cuts at: 5929 96
Size: 138
Fragments arranged by size:
1838 1712 1443 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 3203 1316
Size: 1887 4084
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2832 2905 2832
Size: 73 5898
XhoI C'TCGA_G
Cuts at: 1943 2394 1943
Size: 451 5520
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI