EIF2B4 ORF clone FHC13782

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Halotag ORF Clone FHC13782

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Clone info.

Species

human

Clone Name

pFN21AE5651 Sequence (with vector) Restriction Map

Product ID

FHC13782

ORF Sequence

DNA sequence (1569 bp)
Protein sequence (523 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5651

Symbol

EIF2B4

Alias : EIF-2B, EIF2B, EIF2Bdelta

Description

eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa, transcript variant 2

Original Clone ID

oc95651

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1569 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 523 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33164.1 1.5e-144 100.0 EIF2B4 CCDS46244.1 8.3e-144 99.8 EIF2B4 CCDS82432.1 4.1e-142 100.0 EIF2B4 CCDS46245.1 2.3e-141 99.8 EIF2B4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000649 219 508 PF01008 Initiation factor 2B-related
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001029288 523 603896,606687 100.0 100.0 NP_056451 522 603896,606687 99.8 100.0 NP_001305894 544 603896,606687 100.0 98.1 NP_751945 543 603896,606687 99.8 98.1 NP_001305895 508 603896,606687 100.0 97.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5651 Download

>KIEE5651  1569 bp
ATGGCTGCTGTGGCCGTGGCTGTTCGCGAGGACTCGGGATCCGGGATGAAGGCGGAGCTT
CCCCCTGGGCCTGGGGCAGTGGGGAGGGAAATGACCAAAGAAGAAAAGCTGCAGCTTCGG
AAGGAAAAGAAACAGCAGAAGAAGAAACGGAAGGAAGAAAAGGGGGCAGAACCAGAGACT
GGCTCTGCTGTATCTGCAGCCCAATGTCAAGTAGGCCCAACCAGAGAACTGCCAGAATCG
GGCATTCAGTTGGGCACTCCTCGGGAGAAAGTTCCAGCTGGTCGGAGTAAGGCCGAACTT
CGGGCTGAGCGTCGAGCCAAGCAGGAGGCCGAGCGGGCCCTGAAACAGGCAAGAAAAGGG
GAACAAGGAGGACCACCTCCTAAGGCCAGCCCCAGCACAGCTGGAGAAACCCCCTCAGGA
GTGAAGCGTCTCCCTGAGTACCCTCAGGTTGATGACCTACTTCTGAGAAGGCTTGTTAAA
AAACCAGAGCGTCAACAGGTTCCTACACGAAAGGATTATGGATCCAAAGTCAGTCTCTTC
TCTCACCTACCCCAGTACAGCAGACAAAACTCTCTGACCCAGTTTATGAGCATCCCATCC
TCTGTGATCCACCCAGCCATGGTGCGACTCGGCCTGCAGTACTCCCAGGGCCTGGTCAGT
GGCTCCAATGCCCGGTGTATTGCCCTGCTTCGTGCCTTGCAGCAGGTGATTCAGGATTAC
ACAACACCGCCTAATGAAGAACTCTCCAGGGATCTAGTGAATAAACTAAAACCCTACATG
AGCTTCCTGACTCAGTGCCGTCCCCTGTCAGCGAGCATGCACAACGCCATCAAGTTCCTT
AACAAGGAAATCACCAGTGTGGGCAGTTCCAAGCGGGAAGAGGAGGCCAAGTCAGAACTT
CGAGCAGCCATTGATCGGTATGTGCAAGAGAAGATTGTGCTAGCAGCTCAGGCAATTTCA
CGCTTTGCTTACCAGAAGATCAGTAATGGAGATGTGATCCTGGTATATGGATGCTCATCT
CTGGTATCACGAATTCTTCAGGAGGCTTGGACAGAGGGCCGGCGGTTTCGGGTGGTAGTG
GTGGACAGCCGGCCATGGCTGGAAGGAAGGCACACACTACGTTCTCTAGTCCATGCTGGT
GTCCCAGCCTCCTACCTGCTGATTCCTGCAGCCTCCTATGTGCTCCCAGAGGTTTCCAAG
GTGCTATTGGGAGCTCATGCACTCTTGGCCAACGGGTCTGTGATGTCACGGGTAGGGACA
GCACAGTTAGCCCTGGTGGCTCGAGCCCATAATGTACCAGTGCTGGTTTGCTGTGAAACA
TACAAGTTCTGTGAGCGTGTGCAGACTGATGCCTTTGTCTCTAATGAGCTAGATGACCCT
GATGATCTGCAATGTAAGCGGGGAGAACATGTTGCGCTGGCTAACTGGCAGAACCACGCA
TCCCTACGGTTGTTGAATCTAGTCTATGATGTGACTCCCCCAGAGCTTGTGGATCTGGTG
ATCACGGAGCTGGGGATGATCCCTTGCAGTTCTGTACCTGTTGTTCTACGAGTCAAGAGC
AGTGACCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE5651 Download

>KIEE5651  523 aa
MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPET
GSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKG
EQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLF
SHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDY
TTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSEL
RAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFRVVV
VDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGT
AQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQNHA
SLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5651 Download

>pFN21AE5651  6274 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGCTGTGGCCGTGGCTGTTCGCGAGGACTC
GGGATCCGGGATGAAGGCGGAGCTTCCCCCTGGGCCTGGGGCAGTGGGGAGGGAAATGAC
CAAAGAAGAAAAGCTGCAGCTTCGGAAGGAAAAGAAACAGCAGAAGAAGAAACGGAAGGA
AGAAAAGGGGGCAGAACCAGAGACTGGCTCTGCTGTATCTGCAGCCCAATGTCAAGTAGG
CCCAACCAGAGAACTGCCAGAATCGGGCATTCAGTTGGGCACTCCTCGGGAGAAAGTTCC
AGCTGGTCGGAGTAAGGCCGAACTTCGGGCTGAGCGTCGAGCCAAGCAGGAGGCCGAGCG
GGCCCTGAAACAGGCAAGAAAAGGGGAACAAGGAGGACCACCTCCTAAGGCCAGCCCCAG
CACAGCTGGAGAAACCCCCTCAGGAGTGAAGCGTCTCCCTGAGTACCCTCAGGTTGATGA
CCTACTTCTGAGAAGGCTTGTTAAAAAACCAGAGCGTCAACAGGTTCCTACACGAAAGGA
TTATGGATCCAAAGTCAGTCTCTTCTCTCACCTACCCCAGTACAGCAGACAAAACTCTCT
GACCCAGTTTATGAGCATCCCATCCTCTGTGATCCACCCAGCCATGGTGCGACTCGGCCT
GCAGTACTCCCAGGGCCTGGTCAGTGGCTCCAATGCCCGGTGTATTGCCCTGCTTCGTGC
CTTGCAGCAGGTGATTCAGGATTACACAACACCGCCTAATGAAGAACTCTCCAGGGATCT
AGTGAATAAACTAAAACCCTACATGAGCTTCCTGACTCAGTGCCGTCCCCTGTCAGCGAG
CATGCACAACGCCATCAAGTTCCTTAACAAGGAAATCACCAGTGTGGGCAGTTCCAAGCG
GGAAGAGGAGGCCAAGTCAGAACTTCGAGCAGCCATTGATCGGTATGTGCAAGAGAAGAT
TGTGCTAGCAGCTCAGGCAATTTCACGCTTTGCTTACCAGAAGATCAGTAATGGAGATGT
GATCCTGGTATATGGATGCTCATCTCTGGTATCACGAATTCTTCAGGAGGCTTGGACAGA
GGGCCGGCGGTTTCGGGTGGTAGTGGTGGACAGCCGGCCATGGCTGGAAGGAAGGCACAC
ACTACGTTCTCTAGTCCATGCTGGTGTCCCAGCCTCCTACCTGCTGATTCCTGCAGCCTC
CTATGTGCTCCCAGAGGTTTCCAAGGTGCTATTGGGAGCTCATGCACTCTTGGCCAACGG
GTCTGTGATGTCACGGGTAGGGACAGCACAGTTAGCCCTGGTGGCTCGAGCCCATAATGT
ACCAGTGCTGGTTTGCTGTGAAACATACAAGTTCTGTGAGCGTGTGCAGACTGATGCCTT
TGTCTCTAATGAGCTAGATGACCCTGATGATCTGCAATGTAAGCGGGGAGAACATGTTGC
GCTGGCTAACTGGCAGAACCACGCATCCCTACGGTTGTTGAATCTAGTCTATGATGTGAC
TCCCCCAGAGCTTGTGGATCTGGTGATCACGGAGCTGGGGATGATCCCTTGCAGTTCTGT
ACCTGTTGTTCTACGAGTCAAGAGCAGTGACCAGGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT