SHF ORF clone FHC11385

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11385

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Clone info.

Species

human

Clone Name

pFN21AE0736 Sequence (with vector) Restriction Map

Product ID

FHC11385

Flexi Clone PID

FXC11385

ORF Sequence

DNA sequence (1269 bp)
Protein sequence (423 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528975]

Gene

KIEE0736

Symbol

SHF

Description

Src homology 2 domain containing F, transcript variant 2

Original Clone ID

cs02236

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1269 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 423 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10120.2 5.8e-100 99.8 SHF CCDS73721.1 8e-34 83.2 SHF CCDS76750.1 2.9e-24 64.9 SHF CCDS76749.1 7e-24 72.0 SHF CCDS43806.1 3.4e-20 44.1 SHB The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000980 323 337 PR00401 SH2 motif IPR000980 342 352 PR00401 SH2 motif IPR000980 354 365 PR00401 SH2 motif IPR000980 388 402 PR00401 SH2 motif HMMPfam IPR000980 323 399 PF00017 SH2 motif HMMSmart IPR000980 321 405 SM00252 SH2 motif ProfileScan IPR000980 323 418 PS50001 SH2 motif
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003019 509 600314 44.1 95.0 NP_001010846 495 610482 40.3 87.2 XP_011507465 495 610482 40.3 87.2 NP_064594 340 610481 40.9 84.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0736 Download

>KIEE0736  1269 bp
ATGCAGCAGGAGGGAGGACCCGTGAGGAGCGCCCCCTGTAGGACCGGAACCCTGGAGGGT
TCGCGGCAAGGGTCTCCGGGGCATAGGAAACGGGCATCCCCCAAGGGTAGTCTGAGTTCC
GCCCAGCCCCATTCCTGGATGCTCACGCCGTCTCCCCTAAACTCCCACTGCGCTCACCGG
GAGCCCATCTCATCATCACCACAGCCCGTGGCTAATGGGCCAAAACAGAAGAAGAAAAGC
AACTGGCGGAGTACAACGCGCCTGCGCATTATCAGACTCAGGGACAGGCTGGAACCTCGC
CCCCTAGCTATCCTAGAAGACTATGCGGACCCGTTTGATGTTCAGGAGACTGGCGAAGGC
TCAGCAGGAGCTTCAGGAGCCCCAGAGAAGGTCCCTGAAAATGATGGCTACATGGAGCCC
TATGAGGCTCAAAAGATGATGGCCGAGATCCGGGGCTCCAAGGAGACAGCAACTCAGCCC
TTGCCTCTGTATGACACACCCTATGAGCCAGAGGAGGATGGGGCCACCCCGGAAGGTGAG
GGGGCCCCCTGGCCCCGGGAGTCCCGCCTGCCAGAGGATGATGAGAGGCCCCCTGAGGAG
TATGACCAGCCCTGGGAGTGGAAGAAGGAGCGGATTTCCAAAGCCTTTGCAGTTGACATT
AAGGTCATCAAAGACCTACCTTGGCCTCCACCTGTGGGACAGCTGGACAGCAGCCCCTCC
CTGCCTGATGGGGACAGGGACATCTCCGGTCCAGCCTCGCCCCTCCCTGAGCCCAGCCTG
GAGGACAGCAGCGCCCAGTTTGAAGGACCGGAGAAGAGCTGCCTGTCACCTGGCCGGGAG
GAGAAGGGGCGGCTACCTCCCCGACTCTCTGCAGGGAACCCCAAGTCAGCCAAACCCCTA
AGCATGGAGCCCAGCAGCCCCCTGGGGGAGTGGACAGATCCAGCACTGCCTCTGGAAAAC
CAGGTCTGGTATCACGGGGCCATCAGCCGAACCGACGCCGAGAACCTGCTCCGGCTGTGC
AAAGAGGCCAGCTACCTGGTGCGCAACAGTGAGACCAGCAAGAATGACTTCTCCCTCTCC
CTCAAGAGCAGCCAGGGATTCATGCACATGAAGCTGTCCCGAACCAAGGAACACAAATAT
GTGCTGGGCCAGAACAGCCCGCCCTTCAGCAGCGTCCCTGAAATTGTGCACCACTATGCC
AGCCGCAAGCTACCCATTAAGGGAGCCGAACACATGTCCCTGCTCTACCCTGTGGCCATC
CGGACTCTT

ORF Protein

Cloned ORF protein sequence for pFN21AE0736 Download

>KIEE0736  423 aa
MQQEGGPVRSAPCRTGTLEGSRQGSPGHRKRASPKGSLSSAQPHSWMLTPSPLNSHCAHR
EPISSSPQPVANGPKQKKKSNWRSTTRLRIIRLRDRLEPRPLAILEDYADPFDVQETGEG
SAGASGAPEKVPENDGYMEPYEAQKMMAEIRGSKETATQPLPLYDTPYEPEEDGATPEGE
GAPWPRESRLPEDDERPPEEYDQPWEWKKERISKAFAVDIKVIKDLPWPPPVGQLDSSPS
LPDGDRDISGPASPLPEPSLEDSSAQFEGPEKSCLSPGREEKGRLPPRLSAGNPKSAKPL
SMEPSSPLGEWTDPALPLENQVWYHGAISRTDAENLLRLCKEASYLVRNSETSKNDFSLS
LKSSQGFMHMKLSRTKEHKYVLGQNSPPFSSVPEIVHHYASRKLPIKGAEHMSLLYPVAI
RTL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0736 Download

>pFN21AE0736  5974 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCAGCAGGAGGGAGGACCCGTGAGGAGCGCCCC
CTGTAGGACCGGAACCCTGGAGGGTTCGCGGCAAGGGTCTCCGGGGCATAGGAAACGGGC
ATCCCCCAAGGGTAGTCTGAGTTCCGCCCAGCCCCATTCCTGGATGCTCACGCCGTCTCC
CCTAAACTCCCACTGCGCTCACCGGGAGCCCATCTCATCATCACCACAGCCCGTGGCTAA
TGGGCCAAAACAGAAGAAGAAAAGCAACTGGCGGAGTACAACGCGCCTGCGCATTATCAG
ACTCAGGGACAGGCTGGAACCTCGCCCCCTAGCTATCCTAGAAGACTATGCGGACCCGTT
TGATGTTCAGGAGACTGGCGAAGGCTCAGCAGGAGCTTCAGGAGCCCCAGAGAAGGTCCC
TGAAAATGATGGCTACATGGAGCCCTATGAGGCTCAAAAGATGATGGCCGAGATCCGGGG
CTCCAAGGAGACAGCAACTCAGCCCTTGCCTCTGTATGACACACCCTATGAGCCAGAGGA
GGATGGGGCCACCCCGGAAGGTGAGGGGGCCCCCTGGCCCCGGGAGTCCCGCCTGCCAGA
GGATGATGAGAGGCCCCCTGAGGAGTATGACCAGCCCTGGGAGTGGAAGAAGGAGCGGAT
TTCCAAAGCCTTTGCAGTTGACATTAAGGTCATCAAAGACCTACCTTGGCCTCCACCTGT
GGGACAGCTGGACAGCAGCCCCTCCCTGCCTGATGGGGACAGGGACATCTCCGGTCCAGC
CTCGCCCCTCCCTGAGCCCAGCCTGGAGGACAGCAGCGCCCAGTTTGAAGGACCGGAGAA
GAGCTGCCTGTCACCTGGCCGGGAGGAGAAGGGGCGGCTACCTCCCCGACTCTCTGCAGG
GAACCCCAAGTCAGCCAAACCCCTAAGCATGGAGCCCAGCAGCCCCCTGGGGGAGTGGAC
AGATCCAGCACTGCCTCTGGAAAACCAGGTCTGGTATCACGGGGCCATCAGCCGAACCGA
CGCCGAGAACCTGCTCCGGCTGTGCAAAGAGGCCAGCTACCTGGTGCGCAACAGTGAGAC
CAGCAAGAATGACTTCTCCCTCTCCCTCAAGAGCAGCCAGGGATTCATGCACATGAAGCT
GTCCCGAACCAAGGAACACAAATATGTGCTGGGCCAGAACAGCCCGCCCTTCAGCAGCGT
CCCTGAAATTGTGCACCACTATGCCAGCCGCAAGCTACCCATTAAGGGAGCCGAACACAT
GTCCCTGCTCTACCCTGTGGCCATCCGGACTCTTGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT