ECM2 ORF clone FHC10698

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Halotag ORF Clone FHC10698

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Clone info.

Species

human

Clone Name

pFN21AE0009 Sequence (with vector) Restriction Map

Product ID

FHC10698

Flexi Clone PID

FXC10698

ORF Sequence

DNA sequence (1929 bp)
Protein sequence (643 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527312]

Gene

KIEE0009

Symbol

ECM2

Description

extracellular matrix protein 2, female organ and adipocyte specific, transcript variant 3

Original Clone ID

oc00709

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1929 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 643 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS56578.1 1.4e-134 99.8 ECM2 CCDS6698.1 4.8e-94 96.1 ECM2 CCDS55601.1 4.7e-12 30.3 PODN CCDS55602.1 8.6e-11 31.2 PODN CCDS573.1 8.8e-11 31.2 PODN The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001007 103 157 PF00093 von Willebrand factor IPR001611 346 368 PF00560 Leucine-rich repeat IPR001611 514 531 PF00560 Leucine-rich repeat IPR001611 587 606 PF00560 Leucine-rich repeat HMMSmart IPR001007 103 157 SM00214 von Willebrand factor NULL 320 339 SM00364 NULL IPR003591 321 343 SM00369 Leucine-rich repeat IPR003591 344 369 SM00369 Leucine-rich repeat IPR003591 370 393 SM00369 Leucine-rich repeat NULL 391 415 SM00364 NULL IPR003591 394 414 SM00369 Leucine-rich repeat IPR003591 415 440 SM00369 Leucine-rich repeat IPR003591 441 464 SM00369 Leucine-rich repeat NULL 462 481 SM00364 NULL IPR003591 466 485 SM00369 Leucine-rich repeat NULL 512 531 SM00364 NULL IPR003591 513 535 SM00369 Leucine-rich repeat IPR003591 536 556 SM00369 Leucine-rich repeat IPR003591 585 607 SM00369 Leucine-rich repeat IPR003591 608 631 SM00369 Leucine-rich repeat ProfileScan IPR001007 101 158 PS50184 von Willebrand factor IPR001611 322 343 PS51450 Leucine-rich repeat IPR001611 346 366 PS51450 Leucine-rich repeat IPR001611 372 393 PS51450 Leucine-rich repeat IPR001611 394 414 PS51450 Leucine-rich repeat IPR001611 417 437 PS51450 Leucine-rich repeat IPR001611 464 485 PS51450 Leucine-rich repeat IPR001611 514 535 PS51450 Leucine-rich repeat IPR001611 536 556 PS51450 Leucine-rich repeat IPR001611 560 580 PS51450 Leucine-rich repeat IPR001611 587 608 PS51450 Leucine-rich repeat IPR001611 610 631 PS51450 Leucine-rich repeat
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MKIAVLFCFFLLIIFQTDFGK 21 PRIMARY 21
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001184225 643 603479 99.8 100.0 NP_001184224 677 603479 99.5 97.0 NP_001384 699 603479 96.1 97.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0009 Download

>KIEE0009  1929 bp
ATGAAGATTGCAGTTTTGTTTTGTTTTTTTCTGCTTATCATTTTTCAAACTGACTTTGGA
AAAAATGAAGAAATTCCTAGGAAGCAAAGGAGGAAGATCTACCACAGAAGGTTGAGGAAA
AGTTCAACCTCACACAAGCACAGATCAAACAGACAGCTTGGAATTCCGCAAACAACAGTT
TTTACACCAGTAGCAAGACTTCCTATTGTTAACTTTGATTATAGCATGGAGGAAAAGTTT
GAATCCTTTTCAAGTTTTCCTGGAGTAGAATCAAGTTATAATGTGTTACCAGGAAAGAAG
GGACACTGTTTGGTAAAGGGCATAACCATGTACAACAAAGCTGTGTGGTCGCCTGAGCCC
TGCACTACCTGCCTCTGCTCAGATGGAAGAGTTCTTTGTGATGAAACCATGTGCCATCCC
CAGAGGTGCCCCCAAACAGTTATACCTGAAGGGGAATGCTGCCCGGTCTGCTCCGCTACT
GAACAAAGAGAACCTACCAATTTACTTCATAAGCAACTGCCACCTCCTCAGGTGGGAATG
GACCGAATAGTAAGAAAAGAAGCACTTCAATCTGAGGAGGATGAAGAAGTGAAAGAAGAA
GATACAGAGCAAAAGAGAGAGACCCCTGAATCTAGAAATCAGGGGCAACTTTACAGTGAG
GGGGACAGCAGAGGAGGAGACAGAAAGCAGAGGCCTGGAGAGGAGAGGAGGCTGGCACAC
CAGCAACAACGCCAAGGAAGGGAGGAGGAGGAGGATGAGGAGGAGGAGGGTGAGGAGGGT
GAGGAGGATGAGGAGGACGAGGAGGACCCGGTAAGAGGAGATATGTTCCGAATGCCCTCT
CGATCCCCGCTTCCTGCTCCTCCCAGAGGCACACTGCGCCTGCCAAGCGGGTGCTCTCTG
TCCTACAGGACCATCAGCTGCATCAACGCCATGCTTACCCAGATACCACCGCTGACAGCA
CCACAGATAACAAGTCTGGAGCTCACTGGCAATTCCATCGCCTCCATCCCAGATGAAGCA
TTTAATGGATTACCAAATTTGGAAAGGCTTGATCTGAGTAAAAATAATATCACTTCTTCA
GGCATAGGTCCAAAAGCATTCAAGCTTCTGAAGAAGTTAATGCGTTTGAATATGGATGGA
AATAATTTGATACAGATTCCTTCACAATTGCCATCTACATTAGAAGAACTTAAAGTCAAT
GAGAACAATCTTCAGGCTATCGATGAAGAAAGTTTATCGGACTTAAATCAGTTGGTCACC
TTAGAATTGGAAGGAAACAATCTCAGTGAAGCCAATGTCAATCCTTTAGCTTTCAAACCT
TTGAAGAGCCTAGCCTACTTGCGTCTGGGAAAAAATAAATTTAGAATTATACCGCAGGGT
CTTCCTGGTTCTATTGAGGAATTATACCTAGAAAATAACCAAATTGAAGAAATAACTGAA
ATTTGTTTCAATCATACCAGAAAGATCAATGTCATTGTACTACGTTATAACAAAATTGAA
GAAAATAGGATTGCTCCTTTAGCCTGGATAAATCAAGAAAATCTAGAATCCATTGATCTC
TCCTACAACAAGCTCTATCACGTCCCGTCCTATCTACCCAAGTCCTTGCTGCACCTAGTA
CTCCTTGGGAACCAGATTGAACGGATCCCTGGCTATGTGTTTGGCCACATGGAACCAGGC
CTGGAATACTTGTACCTGTCATTTAACAAACTTGCTGATGATGGCATGGACCGTGTCTCC
TTCTATGGGGCATATCATTCTCTGAGAGAATTATTTCTGGATCACAATGACTTAAAATCT
ATACCACCTGGGATACAAGAAATGAAAGCACTACATTTTCTGAGGCTGAACAACAACAAG
ATACGCTGTGTTTCAGATGCTGTTCTAGAGACAGTGACAAACAGATCAGATGTGGCCTTT
CCTCTCTGG

ORF Protein

Cloned ORF protein sequence for pFN21AE0009 Download

>KIEE0009  643 aa
MKIAVLFCFFLLIIFQTDFGKNEEIPRKQRRKIYHRRLRKSSTSHKHRSNRQLGIPQTTV
FTPVARLPIVNFDYSMEEKFESFSSFPGVESSYNVLPGKKGHCLVKGITMYNKAVWSPEP
CTTCLCSDGRVLCDETMCHPQRCPQTVIPEGECCPVCSATEQREPTNLLHKQLPPPQVGM
DRIVRKEALQSEEDEEVKEEDTEQKRETPESRNQGQLYSEGDSRGGDRKQRPGEERRLAH
QQQRQGREEEEDEEEEGEEGEEDEEDEEDPVRGDMFRMPSRSPLPAPPRGTLRLPSGCSL
SYRTISCINAMLTQIPPLTAPQITSLELTGNSIASIPDEAFNGLPNLERLDLSKNNITSS
GIGPKAFKLLKKLMRLNMDGNNLIQIPSQLPSTLEELKVNENNLQAIDEESLSDLNQLVT
LELEGNNLSEANVNPLAFKPLKSLAYLRLGKNKFRIIPQGLPGSIEELYLENNQIEEITE
ICFNHTRKINVIVLRYNKIEENRIAPLAWINQENLESIDLSYNKLYHVPSYLPKSLLHLV
LLGNQIERIPGYVFGHMEPGLEYLYLSFNKLADDGMDRVSFYGAYHSLRELFLDHNDLKS
IPPGIQEMKALHFLRLNNNKIRCVSDAVLETVTNRSDVAFPLW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0009 Download

>pFN21AE0009  6634 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGATTGCAGTTTTGTTTTGTTTTTTTCTGCT
TATCATTTTTCAAACTGACTTTGGAAAAAATGAAGAAATTCCTAGGAAGCAAAGGAGGAA
GATCTACCACAGAAGGTTGAGGAAAAGTTCAACCTCACACAAGCACAGATCAAACAGACA
GCTTGGAATTCCGCAAACAACAGTTTTTACACCAGTAGCAAGACTTCCTATTGTTAACTT
TGATTATAGCATGGAGGAAAAGTTTGAATCCTTTTCAAGTTTTCCTGGAGTAGAATCAAG
TTATAATGTGTTACCAGGAAAGAAGGGACACTGTTTGGTAAAGGGCATAACCATGTACAA
CAAAGCTGTGTGGTCGCCTGAGCCCTGCACTACCTGCCTCTGCTCAGATGGAAGAGTTCT
TTGTGATGAAACCATGTGCCATCCCCAGAGGTGCCCCCAAACAGTTATACCTGAAGGGGA
ATGCTGCCCGGTCTGCTCCGCTACTGAACAAAGAGAACCTACCAATTTACTTCATAAGCA
ACTGCCACCTCCTCAGGTGGGAATGGACCGAATAGTAAGAAAAGAAGCACTTCAATCTGA
GGAGGATGAAGAAGTGAAAGAAGAAGATACAGAGCAAAAGAGAGAGACCCCTGAATCTAG
AAATCAGGGGCAACTTTACAGTGAGGGGGACAGCAGAGGAGGAGACAGAAAGCAGAGGCC
TGGAGAGGAGAGGAGGCTGGCACACCAGCAACAACGCCAAGGAAGGGAGGAGGAGGAGGA
TGAGGAGGAGGAGGGTGAGGAGGGTGAGGAGGATGAGGAGGACGAGGAGGACCCGGTAAG
AGGAGATATGTTCCGAATGCCCTCTCGATCCCCGCTTCCTGCTCCTCCCAGAGGCACACT
GCGCCTGCCAAGCGGGTGCTCTCTGTCCTACAGGACCATCAGCTGCATCAACGCCATGCT
TACCCAGATACCACCGCTGACAGCACCACAGATAACAAGTCTGGAGCTCACTGGCAATTC
CATCGCCTCCATCCCAGATGAAGCATTTAATGGATTACCAAATTTGGAAAGGCTTGATCT
GAGTAAAAATAATATCACTTCTTCAGGCATAGGTCCAAAAGCATTCAAGCTTCTGAAGAA
GTTAATGCGTTTGAATATGGATGGAAATAATTTGATACAGATTCCTTCACAATTGCCATC
TACATTAGAAGAACTTAAAGTCAATGAGAACAATCTTCAGGCTATCGATGAAGAAAGTTT
ATCGGACTTAAATCAGTTGGTCACCTTAGAATTGGAAGGAAACAATCTCAGTGAAGCCAA
TGTCAATCCTTTAGCTTTCAAACCTTTGAAGAGCCTAGCCTACTTGCGTCTGGGAAAAAA
TAAATTTAGAATTATACCGCAGGGTCTTCCTGGTTCTATTGAGGAATTATACCTAGAAAA
TAACCAAATTGAAGAAATAACTGAAATTTGTTTCAATCATACCAGAAAGATCAATGTCAT
TGTACTACGTTATAACAAAATTGAAGAAAATAGGATTGCTCCTTTAGCCTGGATAAATCA
AGAAAATCTAGAATCCATTGATCTCTCCTACAACAAGCTCTATCACGTCCCGTCCTATCT
ACCCAAGTCCTTGCTGCACCTAGTACTCCTTGGGAACCAGATTGAACGGATCCCTGGCTA
TGTGTTTGGCCACATGGAACCAGGCCTGGAATACTTGTACCTGTCATTTAACAAACTTGC
TGATGATGGCATGGACCGTGTCTCCTTCTATGGGGCATATCATTCTCTGAGAGAATTATT
TCTGGATCACAATGACTTAAAATCTATACCACCTGGGATACAAGAAATGAAAGCACTACA
TTTTCTGAGGCTGAACAACAACAAGATACGCTGTGTTTCAGATGCTGTTCTAGAGACAGT
GACAAACAGATCAGATGTGGCCTTTCCTCTCTGGGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT