ELAVL2 ORF clone FHC10524

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10524

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Clone info.

Species

human

Clone Name

pFN21AB4172 Sequence (with vector) Restriction Map

Product ID

FHC10524

Flexi Clone PID

FXC10524

ORF Sequence

DNA sequence (1077 bp)
Protein sequence (359 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI
Because the PmeI site of this clone is affected by EcoKI methylase, we strongly recommend use of EcoKI methylase-free E.coli strain for preparation of this clone.

GenBank Accession

ORF sequence is derived from [AB528602]

Gene

KIBB4172

Symbol

ELAVL2

Alias : HEL-N1, HELN1, HUB

Description

ELAV like neuron-specific RNA binding protein 2, transcript variant 1

Original Clone ID

fk07158

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1077 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 359 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6515.1 4.5e-106 100.0 ELAVL2 CCDS44138.1 2.3e-96 90.0 ELAVL4 CCDS44140.1 2.3e-96 90.0 ELAVL4 CCDS53315.1 2.3e-96 90.0 ELAVL4 CCDS72788.1 2.3e-96 90.0 ELAVL4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002343 39 54 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 96 111 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 112 129 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 130 145 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 145 157 PR00961 Paraneoplastic encephalomyelitis antigen IPR002343 186 203 PR00961 Paraneoplastic encephalomyelitis antigen HMMPfam IPR000504 41 111 PF00076 RNA recognition motif domain IPR000504 127 193 PF00076 RNA recognition motif domain IPR000504 278 347 PF00076 RNA recognition motif domain HMMSmart IPR000504 40 113 SM00360 RNA recognition motif domain IPR000504 126 201 SM00360 RNA recognition motif domain IPR000504 277 350 SM00360 RNA recognition motif domain HMMTigr IPR006548 36 358 TIGR01661 Splicing factor ELAV/HuD ProfileScan IPR000504 39 117 PS50102 RNA recognition motif domain IPR000504 125 205 PS50102 RNA recognition motif domain IPR000504 276 354 PS50102 RNA recognition motif domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004423 359 601673 100.0 100.0 XP_016869912 359 601673 100.0 100.0 XP_016869911 359 601673 100.0 100.0 XP_016869910 359 601673 100.0 100.0 XP_016869909 359 601673 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4172 Download

>KIBB4172  1077 bp
ATGGAAACACAACTGTCTAATGGGCCAACTTGCAATAACACAGCCAATGGTCCAACCACC
ATAAACAACAACTGTTCGTCACCAGTTGACTCTGGGAACACAGAAGACAGCAAGACCAAC
TTAATAGTCAACTACCTTCCTCAGAACATGACACAGGAGGAACTAAAGAGTCTCTTTGGG
AGCATTGGTGAAATAGAGTCCTGTAAGCTTGTAAGAGACAAAATAACAGGGCAGAGCTTG
GGATATGGCTTTGTGAACTACATTGACCCCAAGGATGCAGAGAAAGCTATCAACACCCTG
AATGGATTGAGACTTCAAACCAAAACAATAAAAGTTTCCTATGCTCGCCCAAGTTCAGCT
TCTATCAGAGATGCAAATTTATATGTCAGCGGACTTCCAAAAACAATGACCCAGAAGGAG
TTGGAACAGCTTTTTTCACAATATGGACGCATTATTACTTCTCGTATTCTTGTCGACCAG
GTCACTGGCATATCAAGGGGTGTAGGGTTTATTCGATTTGACAAGCGAATTGAGGCAGAA
GAAGCTATCAAAGGCCTAAATGGCCAGAAACCTCCCGGTGCCACGGAGCCAATCACTGTA
AAGTTTGCTAATAACCCAAGCCAAAAAACCAATCAGGCCATCCTTTCCCAGCTGTACCAG
TCTCCAAACAGAAGGTATCCAGGACCGCTAGCTCAGCAGGCACAGCGTTTTAGGTTGGAC
AATCTGCTCAATATGGCTTATGGAGTAAAGAGGTTTTCTCCAATGACCATTGACGGAATG
ACCAGTTTGGCTGGAATTAATATCCCTGGGCACCCTGGAACAGGGTGGTGTATATTTGTG
TACAACCTGGCTCCTGACGCAGATGAGAGTATCCTGTGGCAAATGTTTGGGCCTTTTGGA
GCTGTCACCAATGTGAAGGTCATCCGTGACTTTAACACCAATAAATGCAAAGGTTTTGGA
TTTGTGACTATGACAAACTATGATGAGGCTGCCATGGCGATAGCTAGCCTCAATGGATAC
CGTCTGGGAGACAGAGTACTGCAGGTCTCCTTTAAGACAAACAAAACGCACAAAGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB4172 Download

>KIBB4172  359 aa
METQLSNGPTCNNTANGPTTINNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFG
SIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSA
SIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAE
EAIKGLNGQKPPGATEPITVKFANNPSQKTNQAILSQLYQSPNRRYPGPLAQQAQRFRLD
NLLNMAYGVKRFSPMTIDGMTSLAGINIPGHPGTGWCIFVYNLAPDADESILWQMFGPFG
AVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4172 Download

>pFN21AB4172  5782 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAAACACAACTGTCTAATGGGCCAACTTGCAA
TAACACAGCCAATGGTCCAACCACCATAAACAACAACTGTTCGTCACCAGTTGACTCTGG
GAACACAGAAGACAGCAAGACCAACTTAATAGTCAACTACCTTCCTCAGAACATGACACA
GGAGGAACTAAAGAGTCTCTTTGGGAGCATTGGTGAAATAGAGTCCTGTAAGCTTGTAAG
AGACAAAATAACAGGGCAGAGCTTGGGATATGGCTTTGTGAACTACATTGACCCCAAGGA
TGCAGAGAAAGCTATCAACACCCTGAATGGATTGAGACTTCAAACCAAAACAATAAAAGT
TTCCTATGCTCGCCCAAGTTCAGCTTCTATCAGAGATGCAAATTTATATGTCAGCGGACT
TCCAAAAACAATGACCCAGAAGGAGTTGGAACAGCTTTTTTCACAATATGGACGCATTAT
TACTTCTCGTATTCTTGTCGACCAGGTCACTGGCATATCAAGGGGTGTAGGGTTTATTCG
ATTTGACAAGCGAATTGAGGCAGAAGAAGCTATCAAAGGCCTAAATGGCCAGAAACCTCC
CGGTGCCACGGAGCCAATCACTGTAAAGTTTGCTAATAACCCAAGCCAAAAAACCAATCA
GGCCATCCTTTCCCAGCTGTACCAGTCTCCAAACAGAAGGTATCCAGGACCGCTAGCTCA
GCAGGCACAGCGTTTTAGGTTGGACAATCTGCTCAATATGGCTTATGGAGTAAAGAGGTT
TTCTCCAATGACCATTGACGGAATGACCAGTTTGGCTGGAATTAATATCCCTGGGCACCC
TGGAACAGGGTGGTGTATATTTGTGTACAACCTGGCTCCTGACGCAGATGAGAGTATCCT
GTGGCAAATGTTTGGGCCTTTTGGAGCTGTCACCAATGTGAAGGTCATCCGTGACTTTAA
CACCAATAAATGCAAAGGTTTTGGATTTGTGACTATGACAAACTATGATGAGGCTGCCAT
GGCGATAGCTAGCCTCAATGGATACCGTCTGGGAGACAGAGTACTGCAGGTCTCCTTTAA
GACAAACAAAACGCACAAAGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT