CAST ORF clone FHC10482

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10482

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Clone info.

Species

human

Clone Name

pFN21AB9389 Sequence (with vector) Restriction Map

Product ID

FHC10482

Flexi Clone PID

FXC10482

ORF Sequence

DNA sequence (2085 bp)
Protein sequence (695 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527289]

Gene

KIBB9389

Symbol

CAST

Alias : BS-17, PLACK

Description

calpastatin, transcript variant 11

Original Clone ID

hh01300a

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2085 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 695 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS54883.1 5.5e-149 99.9 CAST CCDS83017.1 3.1e-142 99.7 CAST CCDS54882.1 5.4e-107 95.0 CAST CCDS75279.1 6.5e-107 90.4 CAST CCDS4082.1 7.1e-105 97.8 CAST The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001259 128 210 PF00748 Proteinase inhibitor I27 IPR001259 219 345 PF00748 Proteinase inhibitor I27 IPR001259 356 487 PF00748 Proteinase inhibitor I27 IPR001259 499 624 PF00748 Proteinase inhibitor I27
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001177371 695 114090,616295 99.9 100.0 NP_001317560 695 114090,616295 99.9 100.0 NP_001317558 763 114090,616295 99.9 100.0 XP_006714761 778 114090,616295 99.9 100.0 XP_016865417 694 114090,616295 99.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9389 Download

>KIBB9389  2085 bp
ATGAATCCCACAGAAACCAAGGCCATTCCAGTCAGCCAACAGATGGAAGGACCACATCTT
CCTAACAAGAAAAAACACAAAAAACAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAG
TCAACCAAGCTGTCTGTGGTTCATGAGAAAAAATCCCAAGAAGGAAAGCCAAAAGAACAC
ACAGAGCCAAAAAGCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGATGCTCACAAT
AAAAAAGCAGTTTCCAGATCAGCTGAACAGCAGCCATCAGAGAAATCAACAGAACCAAAG
ACTAAACCACAAGACATGATTTCTGCTGGTGGAGAGAGTGTTGCTGGTATCACTGCAATA
TCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAGAAATCATTAACCCCAGCTGTGCCAGTT
GAATCTAAACCGGATAAACCATCGGGAAAGTCAGGCATGGATGCTGCTTTGGATGACTTA
ATAGATACTTTAGGAGGACCTGAAGAAACTGAAGAAGAAAATACAACGTATACTGGACCA
GAAGTTTCAGATCCAATGAGTTCCACCTACATAGAGGAATTGGGTAAAAGAGAAGTCACA
ATTCCTCCAAAATATAGGGAACTATTGGCTAAACCCATAGGGCCAGATGATGCTATAGAC
GCCTTGTCATCTGACTTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAACTGAAAAA
GAGGAATCTACAGAAGTTTTAAAAGCTCAGTCAGCAGGGACAGTCAGAAGTGCTGCTCCA
CCCCAAGAGAAGAAAAGAAAGGTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCT
CTGTCGGCTTCACTGGGCACCCGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAG
GAAGTCGATGAGGCAAAAGCTAAAGAAGAAAAACTAGAGAAGTGTGGTGAGGATGATGAA
ACAATCCCATCTGAGTACAGATTAAAACCAGCCACGGATAAAGATGGAAAACCACTATTG
CCAGAGCCTGAAGAAAAACCCAAGCCTCGGAGTGAATCAGAACTCATTGATGAACTTTCA
GAAGATTTTGACCGGTCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAAGACAGAA
GAATCTAAGGCCGCTGCTCCAGCTCCTGTGTCGGAGGCTGTGTCTCGGACCTCCATGTGT
AGTATACAGTCAGCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGATGCT
GTAGAAGCCTTGGCTGATAGCCTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCT
GTGATGGATAAAGTCAAGGAGAAGGCCAAAGAAGAAGACCGTGAAAAGCTTGGTGAAAAA
GAAGAAACAATTCCTCCTGATTATAGATTAGAAGAGGTCAAGGATAAAGATGGAAAGCCA
CTCCTGCCAAAAGAGTCTAAGGAACAGCTTCCACCCATGAGTGAAGACTTCCTTCTGGAT
GCTTTGTCTGAGGACTTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGAAGATGCT
AAACTTGCTGCTGCCATCTCTGAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCT
GGAGCCCCACCCCGTGATACCTCGCAGAGTGACAAAGACCTCGATGATGCCTTGGATAAA
CTCTCTGACAGTCTAGGACAAAGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGAT
AAAGTAAAGGAAAAAGCTAAAGCTGAACATAGAGACAAGCTTGGAGAAAGAGATGACACT
ATCCCACCTGAATACAGACATCTCCTGGATGATAATGGACAGGACAAACCAGTGAAGCCA
CCTACAAAGAAATCAGAGGATTCAAAGAAACCTGCAGATGACCAAGACCCCATTGATGCT
CTCTCAGGAGATCTGGACAGCTGTCCCTCCACTACAGAAACCTCACAGAACACAGCAAAG
GATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAG
GATTCAGCAAAGACAACAGAGGAAACTTCCAAGCCAAAAGATGAC

ORF Protein

Cloned ORF protein sequence for pFN21AB9389 Download

>KIBB9389  695 aa
MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEH
TEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAI
SGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGP
EVSDPMSSTYIEELGKREVTIPPKYRELLAKPIGPDDAIDALSSDFTCGSPTAAGKKTEK
EESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIK
EVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELS
EDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVSRTSMCSIQSAPPEPATLKGTVPDDA
VEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKP
LLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQA
GAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDT
IPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAK
DKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9389 Download

>pFN21AB9389  6790 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAATCCCACAGAAACCAAGGCCATTCCAGTCAG
CCAACAGATGGAAGGACCACATCTTCCTAACAAGAAAAAACACAAAAAACAGGCTGTAAA
AACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCTGTCTGTGGTTCATGAGAAAAAATC
CCAAGAAGGAAAGCCAAAAGAACACACAGAGCCAAAAAGCCTACCCAAGCAGGCATCAGA
TACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGCTGAACAGCAGCC
ATCAGAGAAATCAACAGAACCAAAGACTAAACCACAAGACATGATTTCTGCTGGTGGAGA
GAGTGTTGCTGGTATCACTGCAATATCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAGAA
ATCATTAACCCCAGCTGTGCCAGTTGAATCTAAACCGGATAAACCATCGGGAAAGTCAGG
CATGGATGCTGCTTTGGATGACTTAATAGATACTTTAGGAGGACCTGAAGAAACTGAAGA
AGAAAATACAACGTATACTGGACCAGAAGTTTCAGATCCAATGAGTTCCACCTACATAGA
GGAATTGGGTAAAAGAGAAGTCACAATTCCTCCAAAATATAGGGAACTATTGGCTAAACC
CATAGGGCCAGATGATGCTATAGACGCCTTGTCATCTGACTTCACCTGTGGGTCGCCTAC
AGCTGCTGGAAAGAAAACTGAAAAAGAGGAATCTACAGAAGTTTTAAAAGCTCAGTCAGC
AGGGACAGTCAGAAGTGCTGCTCCACCCCAAGAGAAGAAAAGAAAGGTGGAGAAGGATAC
AATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCACTGGGCACCCGGCAAGCAGAACC
TGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAGGCAAAAGCTAAAGAAGAAAAACT
AGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCTGAGTACAGATTAAAACCAGCCAC
GGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAAGAAAAACCCAAGCCTCGGAGTGA
ATCAGAACTCATTGATGAACTTTCAGAAGATTTTGACCGGTCTGAATGTAAAGAGAAACC
ATCTAAGCCAACTGAAAAGACAGAAGAATCTAAGGCCGCTGCTCCAGCTCCTGTGTCGGA
GGCTGTGTCTCGGACCTCCATGTGTAGTATACAGTCAGCACCCCCTGAGCCGGCTACCTT
GAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTGGCTGATAGCCTGGGGAAAAAGGA
AGCAGATCCAGAAGATGGAAAACCTGTGATGGATAAAGTCAAGGAGAAGGCCAAAGAAGA
AGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATAGATTAGAAGA
GGTCAAGGATAAAGATGGAAAGCCACTCCTGCCAAAAGAGTCTAAGGAACAGCTTCCACC
CATGAGTGAAGACTTCCTTCTGGATGCTTTGTCTGAGGACTTCTCTGGTCCACAAAATGC
TTCATCTCTTAAATTTGAAGATGCTAAACTTGCTGCTGCCATCTCTGAAGTGGTTTCCCA
AACCCCAGCTTCAACGACCCAAGCTGGAGCCCCACCCCGTGATACCTCGCAGAGTGACAA
AGACCTCGATGATGCCTTGGATAAACTCTCTGACAGTCTAGGACAAAGGCAGCCTGACCC
AGATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGCTGAACATAGAGA
CAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGACATCTCCTGGATGATAA
TGGACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGATTCAAAGAAACCTGC
AGATGACCAAGACCCCATTGATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCCACTAC
AGAAACCTCACAGAACACAGCAAAGGATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGC
ACCTAAGAATGGAGGTAAAGCGAAGGATTCAGCAAAGACAACAGAGGAAACTTCCAAGCC
AAAAGATGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT