GIF ORF clone FHC10349

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10349

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Clone info.

Species

human

Clone Name

pFN21AE6310 Sequence (with vector) Restriction Map

Product ID

FHC10349

ORF Sequence

DNA sequence (1251 bp)
Protein sequence (417 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6310

Symbol

GIF

Description

gastric intrinsic factor (vitamin B synthesis)

Original Clone ID

oc96310

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1251 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 417 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7977.1 2.3e-164 100.0 GIF CCDS7978.1 2e-32 33.9 TCN1 CCDS54519.1 1.2e-11 27.8 TCN2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002157 7 313 PF01122 Cobalamin (vitamin B12)-binding transporter
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MAWFALYLLSLLWATAG 17 PRIMARY 17
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005133 417 261000,609342 100.0 100.0 XP_011543241 403 261000,609342 96.6 100.0 NP_001053 433 189905 33.9 92.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6310 Download

>KIEE6310  1251 bp
ATGGCCTGGTTTGCCCTCTACCTCCTGAGCCTTCTCTGGGCTACAGCTGGGACTAGTACC
CAGACCCAGAGTTCATGCTCCGTTCCCTCAGCACAGGAGCCCTTGGTCAATGGAATACAA
GTACTCATGGAGAACTCGGTGACTTCATCAGCCTACCCAAACCCCAGCATCCTGATTGCC
ATGAATCTGGCCGGAGCCTACAACTTGAAGGCCCAGAAGCTCCTGACTTACCAGCTCATG
TCCAGCGACAACAACGATCTAACCATTGGGCAGCTCGGCCTCACCATCATGGCCCTCACC
TCCTCCTGCCGAGACCCTGGGGATAAAGTATCCATTCTACAAAGACAAATGGAGAACTGG
GCACCTTCCAGCCCCAACGCTGAAGCATCAGCCTTCTATGGGCCCAGTCTAGCGATCTTG
GCACTGTGCCAGAAGAACTCTGAGGCGACCTTGCCGATAGCCGTCCGCTTTGCCAAGACC
CTGCTGGCCAACTCCTCTCCCTTCAATGTAGACACAGGAGCAATGGCAACCTTGGCTCTG
ACCTGTATGTACAACAAGATCCCTGTAGGTTCAGAGGAAGGTTACAGATCCCTGTTTGGT
CAGGTACTAAAGGATATTGTGGAGAAAATCAGCATGAAGATCAAAGATAATGGCATCATT
GGAGACATCTACAGTACTGGCCTCGCCATGCAGGCTCTCTCTGTAACACCTGAGCCATCT
AAAAAGGAATGGAACTGCAAGAAGACTACGGATATGATACTCAATGAGATTAAGCAGGGG
AAATTCCACAACCCCATGTCCATTGCTCAAATCCTCCCTTCCCTGAAAGGCAAGACATAC
CTAGATGTGCCCCAGGTCACTTGTAGTCCTGATCATGAGGTACAACCAACTCTACCCAGC
AACCCTGGCCCTGGCCCCACCTCTGCATCTAACATCACTGTCATATACACCATAAATAAC
CAGCTGAGGGGGGTTGAGCTGCTCTTCAACGAGACCATCAATGTTAGTGTGAAAAGTGGG
TCAGTGTTACTTGTTGTCCTAGAGGAAGCACAGCGCAAAAATCCTATGTTCAAATTTGAA
ACCACAATGACATCTTGGGGCCTTGTCGTCTCTTCTATCAACAATATCGCGGAAAATGTT
AATCACAAGACATACTGGCAGTTTCTTAGTGGTGTAACACCTTTGAATGAAGGGGTTGCT
GACTACATACCCTTCAACCACGAGCACATCACAGCCAATTTCACACAGTAC

ORF Protein

Cloned ORF protein sequence for pFN21AE6310 Download

>KIEE6310  417 aa
MAWFALYLLSLLWATAGTSTQTQSSCSVPSAQEPLVNGIQVLMENSVTSSAYPNPSILIA
MNLAGAYNLKAQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKVSILQRQMENW
APSSPNAEASAFYGPSLAILALCQKNSEATLPIAVRFAKTLLANSSPFNVDTGAMATLAL
TCMYNKIPVGSEEGYRSLFGQVLKDIVEKISMKIKDNGIIGDIYSTGLAMQALSVTPEPS
KKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTYLDVPQVTCSPDHEVQPTLPS
NPGPGPTSASNITVIYTINNQLRGVELLFNETINVSVKSGSVLLVVLEEAQRKNPMFKFE
TTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6310 Download

>pFN21AE6310  5956 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCTGGTTTGCCCTCTACCTCCTGAGCCTTCT
CTGGGCTACAGCTGGGACTAGTACCCAGACCCAGAGTTCATGCTCCGTTCCCTCAGCACA
GGAGCCCTTGGTCAATGGAATACAAGTACTCATGGAGAACTCGGTGACTTCATCAGCCTA
CCCAAACCCCAGCATCCTGATTGCCATGAATCTGGCCGGAGCCTACAACTTGAAGGCCCA
GAAGCTCCTGACTTACCAGCTCATGTCCAGCGACAACAACGATCTAACCATTGGGCAGCT
CGGCCTCACCATCATGGCCCTCACCTCCTCCTGCCGAGACCCTGGGGATAAAGTATCCAT
TCTACAAAGACAAATGGAGAACTGGGCACCTTCCAGCCCCAACGCTGAAGCATCAGCCTT
CTATGGGCCCAGTCTAGCGATCTTGGCACTGTGCCAGAAGAACTCTGAGGCGACCTTGCC
GATAGCCGTCCGCTTTGCCAAGACCCTGCTGGCCAACTCCTCTCCCTTCAATGTAGACAC
AGGAGCAATGGCAACCTTGGCTCTGACCTGTATGTACAACAAGATCCCTGTAGGTTCAGA
GGAAGGTTACAGATCCCTGTTTGGTCAGGTACTAAAGGATATTGTGGAGAAAATCAGCAT
GAAGATCAAAGATAATGGCATCATTGGAGACATCTACAGTACTGGCCTCGCCATGCAGGC
TCTCTCTGTAACACCTGAGCCATCTAAAAAGGAATGGAACTGCAAGAAGACTACGGATAT
GATACTCAATGAGATTAAGCAGGGGAAATTCCACAACCCCATGTCCATTGCTCAAATCCT
CCCTTCCCTGAAAGGCAAGACATACCTAGATGTGCCCCAGGTCACTTGTAGTCCTGATCA
TGAGGTACAACCAACTCTACCCAGCAACCCTGGCCCTGGCCCCACCTCTGCATCTAACAT
CACTGTCATATACACCATAAATAACCAGCTGAGGGGGGTTGAGCTGCTCTTCAACGAGAC
CATCAATGTTAGTGTGAAAAGTGGGTCAGTGTTACTTGTTGTCCTAGAGGAAGCACAGCG
CAAAAATCCTATGTTCAAATTTGAAACCACAATGACATCTTGGGGCCTTGTCGTCTCTTC
TATCAACAATATCGCGGAAAATGTTAATCACAAGACATACTGGCAGTTTCTTAGTGGTGT
AACACCTTTGAATGAAGGGGTTGCTGACTACATACCCTTCAACCACGAGCACATCACAGC
CAATTTCACACAGTACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT