DUSP10 ORF clone FHC10264

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10264

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Clone info.

Species

human

Clone Name

pFN21AE1562 Sequence (with vector) Restriction Map

Product ID

FHC10264

ORF Sequence

DNA sequence (1446 bp)
Protein sequence (482 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590432]

Gene

KIEE1562

Symbol

DUSP10

Alias : MKP-5, MKP5

Description

dual specificity phosphatase 10, transcript variant 1

Original Clone ID

oc10085

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1446 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 482 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1528.1 6.9e-175 100.0 DUSP10 CCDS6072.1 4.7e-27 33.7 DUSP4 CCDS4380.1 3e-24 33.7 DUSP1 CCDS2016.1 2.8e-23 34.6 DUSP2 CCDS33766.2 1.8e-21 33.0 DUSP7 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR008343 173 183 PR01764 MAP kinase phosphatase IPR008343 199 211 PR01764 MAP kinase phosphatase IPR020417 326 338 PR01908 Dual specificity phosphatase IPR020417 343 354 PR01908 Dual specificity phosphatase IPR008343 348 358 PR01764 MAP kinase phosphatase IPR008343 370 379 PR01764 MAP kinase phosphatase IPR020417 370 379 PR01908 Dual specificity phosphatase IPR008343 386 398 PR01764 MAP kinase phosphatase IPR020417 386 397 PR01908 Dual specificity phosphatase HMMPfam IPR001763 165 278 PF00581 Rhodanese-like IPR000340 329 460 PF00782 Dual specificity phosphatase HMMSmart IPR001763 158 282 SM00450 Rhodanese-like IPR020422 321 461 SM00195 Dual specificity phosphatase ProfileScan IPR001763 168 285 PS50206 Rhodanese-like IPR020422 321 463 PS50054 Dual specificity phosphatase IPR000387 384 443 PS50056 Protein-tyrosine/Dual-specificity phosphatase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_009138 482 608867 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1562 Download

>KIEE1562  1446 bp
ATGCCTCCGTCTCCTTTAGACGACAGGGTAGTAGTGGCACTATCTAGGCCCGTCCGACCT
CAGGATCTCAACCTTTGTTTAGACTCTAGTTACCTTGGCTCTGCCAACCCAGGCAGTAAC
AGCCACCCTCCTGTCATCGCCACCACCGTTGTGTCCCTCAAGGCTGCGAATCTGACGTAT
ATGCCCTCATCCAGCGGCTCTGCCCGCTCGCTGAATTGTGGATGCAGCAGTGCCAGCTGC
TGCACTGTGGCAACCTACGACAAGGACAATCAGGCCCAAACCCAAGCCATTGCCGCTGGC
ACCACCACCACTGCCATCGGAACCTCTACCACCTGCCCTGCTAACCAGATGGTCAACAAT
AATGAGAATACAGGCTCTCTAAGTCCATCAAGTGGGGTGGGCAGCCCTGTGTCAGGGACC
CCCAAGCAGCTAGCCAGCATCAAAATAATCTACCCCAATGACTTGGCAAAGAAGATGACC
AAATGCAGCAAGAGTCACCTGCCGAGTCAGGGCCCTGTCATCATTGACTGCAGGCCCTTC
ATGGAGTACAACAAGAGTCACATCCAAGGAGCTGTCCACATTAACTGTGCCGATAAGATC
AGCCGGCGGAGACTGCAGCAGGGCAAGATCACTGTCCTAGACTTGATTTCCTGTAGGGAA
GGCAAGGACTCTTTCAAGAGGATCTTTTCCAAAGAAATTATAGTTTATGATGAGAATACC
AATGAACCAAGCCGAGTGATGCCCTCCCAGCCACTTCACATAGTCCTCGAGTCCCTGAAG
AGAGAAGGCAAAGAACCTCTGGTGTTGAAAGGTGGACTTAGTAGTTTTAAGCAGAACCAT
GAAAACCTCTGTGACAACTCCCTCCAGCTCCAAGAGTGCCGGGAGGTGGGGGGCGGCGCA
TCCGCGGCCTCGAGCTTGCTACCTCAGCCCATCCCCACCACCCCTGACATCGAGAACGCT
GAGCTCACCCCCATCTTGCCCTTCCTGTTCCTTGGCAATGAGCAGGATGCTCAGGACCTG
GACACCATGCAGCGGCTGAACATCGGCTACGTCATCAACGTCACCACTCATCTTCCCCTC
TACCACTATGAGAAAGGCCTGTTCAACTACAAGCGGCTGCCAGCCACTGACAGCAACAAG
CAGAACCTGCGGCAGTACTTTGAAGAGGCTTTTGAGTTCATTGAGGAAGCTCACCAGTGT
GGGAAGGGGCTTCTCATCCACTGCCAGGCTGGGGTGTCCCGCTCCGCCACCATCGTCATC
GCTTACTTGATGAAGCACACTCGGATGACCATGACTGATGCTTATAAATTTGTCAAAGGC
AAACGACCAATTATCTCCCCAAACCTTAACTTCATGGGGCAGTTGCTAGAGTTCGAGGAA
GACCTAAACAACGGTGTGACACCGAGAATCCTTACACCAAAGCTGATGGGCGTGGAGACG
GTTGTG

ORF Protein

Cloned ORF protein sequence for pFN21AE1562 Download

>KIEE1562  482 aa
MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSANPGSNSHPPVIATTVVSLKAANLTY
MPSSSGSARSLNCGCSSASCCTVATYDKDNQAQTQAIAAGTTTTAIGTSTTCPANQMVNN
NENTGSLSPSSGVGSPVSGTPKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPF
MEYNKSHIQGAVHINCADKISRRRLQQGKITVLDLISCREGKDSFKRIFSKEIIVYDENT
NEPSRVMPSQPLHIVLESLKREGKEPLVLKGGLSSFKQNHENLCDNSLQLQECREVGGGA
SAASSLLPQPIPTTPDIENAELTPILPFLFLGNEQDAQDLDTMQRLNIGYVINVTTHLPL
YHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIVI
AYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGVET
VV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1562 Download

>pFN21AE1562  6151 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCTCCGTCTCCTTTAGACGACAGGGTAGTAGT
GGCACTATCTAGGCCCGTCCGACCTCAGGATCTCAACCTTTGTTTAGACTCTAGTTACCT
TGGCTCTGCCAACCCAGGCAGTAACAGCCACCCTCCTGTCATCGCCACCACCGTTGTGTC
CCTCAAGGCTGCGAATCTGACGTATATGCCCTCATCCAGCGGCTCTGCCCGCTCGCTGAA
TTGTGGATGCAGCAGTGCCAGCTGCTGCACTGTGGCAACCTACGACAAGGACAATCAGGC
CCAAACCCAAGCCATTGCCGCTGGCACCACCACCACTGCCATCGGAACCTCTACCACCTG
CCCTGCTAACCAGATGGTCAACAATAATGAGAATACAGGCTCTCTAAGTCCATCAAGTGG
GGTGGGCAGCCCTGTGTCAGGGACCCCCAAGCAGCTAGCCAGCATCAAAATAATCTACCC
CAATGACTTGGCAAAGAAGATGACCAAATGCAGCAAGAGTCACCTGCCGAGTCAGGGCCC
TGTCATCATTGACTGCAGGCCCTTCATGGAGTACAACAAGAGTCACATCCAAGGAGCTGT
CCACATTAACTGTGCCGATAAGATCAGCCGGCGGAGACTGCAGCAGGGCAAGATCACTGT
CCTAGACTTGATTTCCTGTAGGGAAGGCAAGGACTCTTTCAAGAGGATCTTTTCCAAAGA
AATTATAGTTTATGATGAGAATACCAATGAACCAAGCCGAGTGATGCCCTCCCAGCCACT
TCACATAGTCCTCGAGTCCCTGAAGAGAGAAGGCAAAGAACCTCTGGTGTTGAAAGGTGG
ACTTAGTAGTTTTAAGCAGAACCATGAAAACCTCTGTGACAACTCCCTCCAGCTCCAAGA
GTGCCGGGAGGTGGGGGGCGGCGCATCCGCGGCCTCGAGCTTGCTACCTCAGCCCATCCC
CACCACCCCTGACATCGAGAACGCTGAGCTCACCCCCATCTTGCCCTTCCTGTTCCTTGG
CAATGAGCAGGATGCTCAGGACCTGGACACCATGCAGCGGCTGAACATCGGCTACGTCAT
CAACGTCACCACTCATCTTCCCCTCTACCACTATGAGAAAGGCCTGTTCAACTACAAGCG
GCTGCCAGCCACTGACAGCAACAAGCAGAACCTGCGGCAGTACTTTGAAGAGGCTTTTGA
GTTCATTGAGGAAGCTCACCAGTGTGGGAAGGGGCTTCTCATCCACTGCCAGGCTGGGGT
GTCCCGCTCCGCCACCATCGTCATCGCTTACTTGATGAAGCACACTCGGATGACCATGAC
TGATGCTTATAAATTTGTCAAAGGCAAACGACCAATTATCTCCCCAAACCTTAACTTCAT
GGGGCAGTTGCTAGAGTTCGAGGAAGACCTAAACAACGGTGTGACACCGAGAATCCTTAC
ACCAAAGCTGATGGGCGTGGAGACGGTTGTGGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT