CAP1 ORF clone FHC10009

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10009

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Clone info.

Species

human

Clone Name

pFN21AE1541 Sequence (with vector) Restriction Map

Product ID

FHC10009

ORF Sequence

DNA sequence (1425 bp)
Protein sequence (475 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590394]

Gene

KIEE1541

Symbol

CAP1

Alias : CAP, CAP1-PEN

Description

CAP, adenylate cyclase-associated protein 1 (yeast), transcript variant 1

Original Clone ID

oc10064

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1425 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 475 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS41309.1 4.4e-95 98.7 CAP1 CCDS81304.1 1.6e-94 98.5 CAP1 CCDS4539.1 1.5e-60 64.6 CAP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013992 4 305 PF01213 Adenylate cyclase-associated CAP IPR013912 317 473 PF08603 Adenylate cyclase-associated CAP HMMSmart IPR006599 356 393 SM00673 CARP motif IPR006599 394 431 SM00673 CARP motif ProfileScan IPR017901 319 453 PS51329 C-CAP/cofactor C-like domain Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1541 Download

>KIEE1541  1425 bp
ATGGCTGACATGCAAAATCTGGTAGAAAGATTGGAGAGGGCAGTGGGCCGCCTGGAGGCA
GTATCTCATACCTCTGACATGCACCGTGGGTATGCAGACAGTCCTTCAAAAGCAGGAGCA
GCTCCATATGTGCAGGCATTTGACTCGCTGCTTGCTGGTCCTGTGGCAGAGTACTTGAAG
ATCAGTAAAGAGATTGGGGGAGACGTGCAGAAACATGCGGAGATGGTCCACACAGGTTTG
AAGTTGGAGCGAGCTCTGTTGGTTACAGCTTCTCAGTGTCAACAGCCAGCAGAAAATAAG
CTTTCCGATTTGTTGGCACCCATCTCAGAGCAGATCAAAGAAGTGATAACCTTTCGGGAG
AAGAACCGAGGCAGCAAGTTGTTTAATCACCTGTCAGCTGTCAGCGAAAGTATCCAGGCC
CTGGGCTGGGTGGCTATGGCTCCCAAGCCTGGCCCTTATGTGAAAGAAATGAATGATGCC
GCCATGTTTTATACAAACCGAGTCCTCAAAGAGTACAAAGATGTGGATAAGAAGCATGTA
GACTGGGTCAAAGCTTATTTAAGTATATGGACAGAGCTGCAGGCTTACATTAAGGAGTTC
CATACCACCGGACTGGCCTGGAGCAAAACGGGGCCTGTGGCAAAAGAACTGAGCGGACTG
CCATCTGGACCCTCTGCCGGATCAGGTCCTCCTCCCCCTCCACCAGGCCCCCCTCCTCCC
CCAGTCTCTACCAGTTCAGGCTCAGATGAGTCTGCTTCCCGCTCAGCACTGTTCGCGCAG
ATTAATCAGGGGGAGAGCATTACACATGCCCTGAAACATGTATCTGATGACATGAAGACT
CACAAGAACCCTGCCCTGAAGGCTCAGAGTGGTCCAGTACGCAGTGGCCCCAAACCATTC
TCTGCACCTAAACCCCAAACCAGCCCATCCCCCAAACGAGCCACAAAGAAGGAGCCAGCT
GTACTTGAACTGGAGGGCAAGAAGTGGAGAGTGGAAAATCAGGAAAATGTTTCCAACCTG
GTGATTGAGGACACAGAGCTGAAACAGGTGGCTTACATATACAAGTGTGTCAACACGACA
TTGCAAATCAAGGGCAAAATTAACTCCATTACAGTAGATAACTGTAAGAAACTTGGCCTG
GTATTCGATGACGTGGTGGGCATTGTGGAGATAATCAACAGTAAGGATGTCAAAGTTCAG
GTAATGGGTAAAGTGCCAACCATATCCATCAACAAAACAGATGGCTGCCATGCTTACCTG
AGCAAGAATTCCCTGGATTGTGAAATAGTCAGTGCCAAATCTTCCGAGATGAATGTCCTC
ATTCCTACAGAAGGCGGTGACTTTAATGAATTCCCAGTTCCTGAGCAGTTCAAGACCCTA
TGGAACGGGCAGAAGTTGGTCACCACAGTGACAGAAATTGCTGGA

ORF Protein

Cloned ORF protein sequence for pFN21AE1541 Download

>KIEE1541  475 aa
MADMQNLVERLERAVGRLEAVSHTSDMHRGYADSPSKAGAAPYVQAFDSLLAGPVAEYLK
ISKEIGGDVQKHAEMVHTGLKLERALLVTASQCQQPAENKLSDLLAPISEQIKEVITFRE
KNRGSKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHV
DWVKAYLSIWTELQAYIKEFHTTGLAWSKTGPVAKELSGLPSGPSAGSGPPPPPPGPPPP
PVSTSSGSDESASRSALFAQINQGESITHALKHVSDDMKTHKNPALKAQSGPVRSGPKPF
SAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQVAYIYKCVNTT
LQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYL
SKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1541 Download

>pFN21AE1541  6130 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGACATGCAAAATCTGGTAGAAAGATTGGA
GAGGGCAGTGGGCCGCCTGGAGGCAGTATCTCATACCTCTGACATGCACCGTGGGTATGC
AGACAGTCCTTCAAAAGCAGGAGCAGCTCCATATGTGCAGGCATTTGACTCGCTGCTTGC
TGGTCCTGTGGCAGAGTACTTGAAGATCAGTAAAGAGATTGGGGGAGACGTGCAGAAACA
TGCGGAGATGGTCCACACAGGTTTGAAGTTGGAGCGAGCTCTGTTGGTTACAGCTTCTCA
GTGTCAACAGCCAGCAGAAAATAAGCTTTCCGATTTGTTGGCACCCATCTCAGAGCAGAT
CAAAGAAGTGATAACCTTTCGGGAGAAGAACCGAGGCAGCAAGTTGTTTAATCACCTGTC
AGCTGTCAGCGAAAGTATCCAGGCCCTGGGCTGGGTGGCTATGGCTCCCAAGCCTGGCCC
TTATGTGAAAGAAATGAATGATGCCGCCATGTTTTATACAAACCGAGTCCTCAAAGAGTA
CAAAGATGTGGATAAGAAGCATGTAGACTGGGTCAAAGCTTATTTAAGTATATGGACAGA
GCTGCAGGCTTACATTAAGGAGTTCCATACCACCGGACTGGCCTGGAGCAAAACGGGGCC
TGTGGCAAAAGAACTGAGCGGACTGCCATCTGGACCCTCTGCCGGATCAGGTCCTCCTCC
CCCTCCACCAGGCCCCCCTCCTCCCCCAGTCTCTACCAGTTCAGGCTCAGATGAGTCTGC
TTCCCGCTCAGCACTGTTCGCGCAGATTAATCAGGGGGAGAGCATTACACATGCCCTGAA
ACATGTATCTGATGACATGAAGACTCACAAGAACCCTGCCCTGAAGGCTCAGAGTGGTCC
AGTACGCAGTGGCCCCAAACCATTCTCTGCACCTAAACCCCAAACCAGCCCATCCCCCAA
ACGAGCCACAAAGAAGGAGCCAGCTGTACTTGAACTGGAGGGCAAGAAGTGGAGAGTGGA
AAATCAGGAAAATGTTTCCAACCTGGTGATTGAGGACACAGAGCTGAAACAGGTGGCTTA
CATATACAAGTGTGTCAACACGACATTGCAAATCAAGGGCAAAATTAACTCCATTACAGT
AGATAACTGTAAGAAACTTGGCCTGGTATTCGATGACGTGGTGGGCATTGTGGAGATAAT
CAACAGTAAGGATGTCAAAGTTCAGGTAATGGGTAAAGTGCCAACCATATCCATCAACAA
AACAGATGGCTGCCATGCTTACCTGAGCAAGAATTCCCTGGATTGTGAAATAGTCAGTGC
CAAATCTTCCGAGATGAATGTCCTCATTCCTACAGAAGGCGGTGACTTTAATGAATTCCC
AGTTCCTGAGCAGTTCAAGACCCTATGGAACGGGCAGAAGTTGGTCACCACAGTGACAGA
AATTGCTGGAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT