CNKSR1 ORF clone FHC09975

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09975

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Clone info.

Species

human

Clone Name

pFN21AB5712 Sequence (with vector) Restriction Map

Product ID

FHC09975

ORF Sequence

DNA sequence (2160 bp)
Protein sequence (720 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590388]

Gene

KIBB5712

Symbol

CNKSR1

Alias : CNK, CNK1

Description

connector enhancer of kinase suppressor of Ras 1, transcript variant 3

Original Clone ID

cs01707

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2160 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 720 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS72732.1 4.7e-153 100.0 CNKSR1 CCDS276.1 8.2e-151 99.0 CNKSR1 CCDS76124.1 1.3e-95 100.0 CNKSR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR021129 6 67 PF00536 Sterile alpha motif IPR019555 78 162 PF10534 CRIC domain IPR001849 406 502 PF00169 Pleckstrin homology domain HMMSmart IPR001660 4 70 SM00454 Sterile alpha motif domain IPR001849 404 504 SM00233 Pleckstrin homology domain ProfileScan IPR001660 7 70 PS50105 Sterile alpha motif domain IPR017874 78 164 PS51290 CRIC domain IPR001478 196 285 PS50106 PDZ/DHR/GLGF IPR001849 403 502 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001284576 720 603272 100.0 100.0 NP_006305 713 603272 99.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5712 Download

>KIBB5712  2160 bp
ATGGAACCGGTAGAGACCTGGACCCCCGGAAAGGTGGCAACTTGGCTGAGAGGTCTTGAC
GACTCCCTGCAGGACTATCCCTTTGAGGACTGGCAGCTGCCTGGCAAGAACCTGCTCCAG
CTCTGCCCCCAAAGCCTCGAGGCTCTGGCTGTGCGGTCTCTGGGACACCAGGAGCTCATC
CTGGGCGGGGTGGAACAGCTCCAGGCCCTGAGCTCCAGGCTACAGACAGAGAACCTGCAA
AGCCTGACAGAGGGACTTCTGGGGGCAACCCATGACTTCCAGAGCATAGTCCAAGGCTGC
CTGGGGGACTGTGCCAAGACCCCTATTGATGTCCTCTGTGCAGCTGTGGAGCTGTTGCAT
GAAGCTGACGCCCTCCTCTTCTGGCTCAGCAGGTACCTCTTCTCCCACTTAAATGATTTC
TCAGCATGCCAGGAGATCCGAGACTTGTTGGAGGAGCTGAGCCAGGTCTTGCATGAGGAT
GGTCCAGCGGCTGAGAAGGAGGGCACAGTCCTGAGGATCTGCAGCCACGTGGCTGGGATC
TGCCACAACATCCTGGTCTGCTGCCCCAAGGAGCTGCTGGAACAGAAGGCCGTGCTCGAG
CAGGTGCAGCTGGACAGTCCATTGGGCCTAGAAATTCACACCACCAGCAATTGCCAGCAC
TTTGTGTCCCAAGTGGACACCCAGGTTCCCACTGACTCCCGACTGCAGATCCAGCCTGGA
GACGAGGTTGTCCAGATCAACGAGCAGGTGGTGGTGCGTGAGGAGAGGGACATGGTGGGA
TGGCCCCGTAAGAACATGGTGAGGGAACTGCTGCGGGAGCCAGCCGGACTCAGCTTAGTG
CTGAAGAAGATCCCGATACCGGAGACCCCCCCACAGACGCCCCCTCAGGTCCTGGACTCC
CCGCACCAGAGGAGCCCATCACTGTCTCTGGCCCCACTGTCTCCCAGGGCCCCATCTGAA
GACGTCTTTGCCTTTGACCTGTCTTCAAACCCCAGTCCCGGACCCAGCCCTGCCTGGACA
GACTCTGCCTCCCTTGGCCCTGAGCCCCTGCCCATCCCCCCGGAACCCCCAGCCATACTC
CCAGCAGGGGTAGCAGGGACTCCAGGGCTCCCTGAATCCCCTGACAAGAGTCCTGTTGGT
CGGAAGAAATCAAAAGGCCTGGCGACCCGGCTGAGCCGCCGGCGGGTGTCATGCCGTGAG
CTGGGCCGGCCGGACTGTGACGGCTGGCTCCTGTTGCGAAAGGCACCGGGCGGCTTCATG
GGCCCGCGCTGGCGCCGCCGCTGGTTTGTGCTCAAGGGACACACGCTCTACTGGTACCGC
CAGCCCCAGGATGAGAAGGCTGAGGGCCTCATCAATGTCTCCAACTATAGTCTGGAAAGT
GGACATGATCAGAAGAAGAAATATGTGTTTCAGCTCACCCATGATGTGTACAAACCCTTC
ATCTTCGCTGCTGATACCCTGACAGATCTGAGCATGTGGGTGCGTCATCTCATTACCTGC
ATCTCCAAGTACCAGTCTCCAGGCCGGGCCCCCCCACCCCGAGAGGAAGACTGCTACAGT
GAGACCGAAGCAGAGGACCCGGACGATGAGGCTGGGTCCCACTCAGCCTCGCCCAGCCCT
GCTCAAGCTGGGAGTCCCCTCCATGGAGACACATCACCTGCAGCCACCCCCACACAGCGC
AGCCCACGGACCTCCTTTGGCTCTCTGACAGACAGCAGTGAAGAGGCACTGGAAGGAATG
GTACGGGGGCTGAGGCAGGGTGGCGTGTCCCTCCTAGGCCAGCCACAGCCCCTGACCCAG
GAACAGTGGCGGAGCTCTTTCATGCGGCGCAACCGAGACCCTCAGCTCAATGAGCGAGTG
CACCGTGTGCGGGCGCTACAGAGCACACTCAAGGCAAAGCTGCAGGAGCTGCAGGTCCTA
GAAGAAGTGCTGGGTGACCCTGAGCTGACAGGAGAGAAGTTCCGCCAGTGGAAGGAGCAG
AACCGGGAGCTGTACTCAGAGGGCCTGGGGGCCTGGGGAGTGGCACAGGCTGAAGGCAGC
TCCCACATCTTGACCTCTGACTCCACAGAACAGTCCCCCCACTCCCTGCCCTCTGACCCT
GAAGAGCACTCCCATCTCTGCCCCCTGACCTCAGAGAGCAGCCTCCGACCTCCTGACCTC

ORF Protein

Cloned ORF protein sequence for pFN21AB5712 Download

>KIBB5712  720 aa
MEPVETWTPGKVATWLRGLDDSLQDYPFEDWQLPGKNLLQLCPQSLEALAVRSLGHQELI
LGGVEQLQALSSRLQTENLQSLTEGLLGATHDFQSIVQGCLGDCAKTPIDVLCAAVELLH
EADALLFWLSRYLFSHLNDFSACQEIRDLLEELSQVLHEDGPAAEKEGTVLRICSHVAGI
CHNILVCCPKELLEQKAVLEQVQLDSPLGLEIHTTSNCQHFVSQVDTQVPTDSRLQIQPG
DEVVQINEQVVVREERDMVGWPRKNMVRELLREPAGLSLVLKKIPIPETPPQTPPQVLDS
PHQRSPSLSLAPLSPRAPSEDVFAFDLSSNPSPGPSPAWTDSASLGPEPLPIPPEPPAIL
PAGVAGTPGLPESPDKSPVGRKKSKGLATRLSRRRVSCRELGRPDCDGWLLLRKAPGGFM
GPRWRRRWFVLKGHTLYWYRQPQDEKAEGLINVSNYSLESGHDQKKKYVFQLTHDVYKPF
IFAADTLTDLSMWVRHLITCISKYQSPGRAPPPREEDCYSETEAEDPDDEAGSHSASPSP
AQAGSPLHGDTSPAATPTQRSPRTSFGSLTDSSEEALEGMVRGLRQGGVSLLGQPQPLTQ
EQWRSSFMRRNRDPQLNERVHRVRALQSTLKAKLQELQVLEEVLGDPELTGEKFRQWKEQ
NRELYSEGLGAWGVAQAEGSSHILTSDSTEQSPHSLPSDPEEHSHLCPLTSESSLRPPDL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5712 Download

>pFN21AB5712  6865 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAACCGGTAGAGACCTGGACCCCCGGAAAGGT
GGCAACTTGGCTGAGAGGTCTTGACGACTCCCTGCAGGACTATCCCTTTGAGGACTGGCA
GCTGCCTGGCAAGAACCTGCTCCAGCTCTGCCCCCAAAGCCTCGAGGCTCTGGCTGTGCG
GTCTCTGGGACACCAGGAGCTCATCCTGGGCGGGGTGGAACAGCTCCAGGCCCTGAGCTC
CAGGCTACAGACAGAGAACCTGCAAAGCCTGACAGAGGGACTTCTGGGGGCAACCCATGA
CTTCCAGAGCATAGTCCAAGGCTGCCTGGGGGACTGTGCCAAGACCCCTATTGATGTCCT
CTGTGCAGCTGTGGAGCTGTTGCATGAAGCTGACGCCCTCCTCTTCTGGCTCAGCAGGTA
CCTCTTCTCCCACTTAAATGATTTCTCAGCATGCCAGGAGATCCGAGACTTGTTGGAGGA
GCTGAGCCAGGTCTTGCATGAGGATGGTCCAGCGGCTGAGAAGGAGGGCACAGTCCTGAG
GATCTGCAGCCACGTGGCTGGGATCTGCCACAACATCCTGGTCTGCTGCCCCAAGGAGCT
GCTGGAACAGAAGGCCGTGCTCGAGCAGGTGCAGCTGGACAGTCCATTGGGCCTAGAAAT
TCACACCACCAGCAATTGCCAGCACTTTGTGTCCCAAGTGGACACCCAGGTTCCCACTGA
CTCCCGACTGCAGATCCAGCCTGGAGACGAGGTTGTCCAGATCAACGAGCAGGTGGTGGT
GCGTGAGGAGAGGGACATGGTGGGATGGCCCCGTAAGAACATGGTGAGGGAACTGCTGCG
GGAGCCAGCCGGACTCAGCTTAGTGCTGAAGAAGATCCCGATACCGGAGACCCCCCCACA
GACGCCCCCTCAGGTCCTGGACTCCCCGCACCAGAGGAGCCCATCACTGTCTCTGGCCCC
ACTGTCTCCCAGGGCCCCATCTGAAGACGTCTTTGCCTTTGACCTGTCTTCAAACCCCAG
TCCCGGACCCAGCCCTGCCTGGACAGACTCTGCCTCCCTTGGCCCTGAGCCCCTGCCCAT
CCCCCCGGAACCCCCAGCCATACTCCCAGCAGGGGTAGCAGGGACTCCAGGGCTCCCTGA
ATCCCCTGACAAGAGTCCTGTTGGTCGGAAGAAATCAAAAGGCCTGGCGACCCGGCTGAG
CCGCCGGCGGGTGTCATGCCGTGAGCTGGGCCGGCCGGACTGTGACGGCTGGCTCCTGTT
GCGAAAGGCACCGGGCGGCTTCATGGGCCCGCGCTGGCGCCGCCGCTGGTTTGTGCTCAA
GGGACACACGCTCTACTGGTACCGCCAGCCCCAGGATGAGAAGGCTGAGGGCCTCATCAA
TGTCTCCAACTATAGTCTGGAAAGTGGACATGATCAGAAGAAGAAATATGTGTTTCAGCT
CACCCATGATGTGTACAAACCCTTCATCTTCGCTGCTGATACCCTGACAGATCTGAGCAT
GTGGGTGCGTCATCTCATTACCTGCATCTCCAAGTACCAGTCTCCAGGCCGGGCCCCCCC
ACCCCGAGAGGAAGACTGCTACAGTGAGACCGAAGCAGAGGACCCGGACGATGAGGCTGG
GTCCCACTCAGCCTCGCCCAGCCCTGCTCAAGCTGGGAGTCCCCTCCATGGAGACACATC
ACCTGCAGCCACCCCCACACAGCGCAGCCCACGGACCTCCTTTGGCTCTCTGACAGACAG
CAGTGAAGAGGCACTGGAAGGAATGGTACGGGGGCTGAGGCAGGGTGGCGTGTCCCTCCT
AGGCCAGCCACAGCCCCTGACCCAGGAACAGTGGCGGAGCTCTTTCATGCGGCGCAACCG
AGACCCTCAGCTCAATGAGCGAGTGCACCGTGTGCGGGCGCTACAGAGCACACTCAAGGC
AAAGCTGCAGGAGCTGCAGGTCCTAGAAGAAGTGCTGGGTGACCCTGAGCTGACAGGAGA
GAAGTTCCGCCAGTGGAAGGAGCAGAACCGGGAGCTGTACTCAGAGGGCCTGGGGGCCTG
GGGAGTGGCACAGGCTGAAGGCAGCTCCCACATCTTGACCTCTGACTCCACAGAACAGTC
CCCCCACTCCCTGCCCTCTGACCCTGAAGAGCACTCCCATCTCTGCCCCCTGACCTCAGA
GAGCAGCCTCCGACCTCCTGACCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT