SPAM1 ORF clone FHC09189

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09189

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Clone info.

Species

human

Clone Name

pFN21AE5612 Sequence (with vector) Restriction Map

Product ID

FHC09189

ORF Sequence

DNA sequence (1533 bp)
Protein sequence (511 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5612

Symbol

SPAM1

Alias : HEL-S-96n, HYA1, HYAL1, HYAL3, HYAL5, PH-20, PH20, SPAG15

Description

sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), transcript variant 1

Original Clone ID

oc95612

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1533 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 511 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5790.1 0 100.0 SPAM1 CCDS5791.1 0 100.0 SPAM1 CCDS5789.1 2.9e-91 45.1 HYAL4 CCDS2818.1 3.7e-77 40.3 HYAL2 CCDS2816.1 3.1e-75 41.6 HYAL1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001439 2 19 PR00848 Glycoside hydrolase IPR001439 19 34 PR00848 Glycoside hydrolase IPR018155 49 61 PR00846 Hyaluronidase IPR001439 70 86 PR00848 Glycoside hydrolase IPR018155 110 124 PR00846 Hyaluronidase IPR018155 141 158 PR00846 Hyaluronidase IPR018155 199 224 PR00846 Hyaluronidase IPR018155 246 260 PR00846 Hyaluronidase IPR018155 351 364 PR00846 Hyaluronidase IPR001439 404 419 PR00848 Glycoside hydrolase IPR001439 419 433 PR00848 Glycoside hydrolase IPR001439 438 452 PR00848 Glycoside hydrolase IPR001439 455 469 PR00848 Glycoside hydrolase HMMPfam IPR018155 38 374 PF01630 Hyaluronidase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003108 511 600930 100.0 100.0 NP_001167517 509 600930 100.0 96.9 NP_001167515 509 600930 100.0 96.9 NP_694859 509 600930 100.0 96.9 NP_001167516 509 600930 100.0 96.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5612 Download

>KIEE5612  1533 bp
ATGGGAGTGCTAAAATTCAAGCACATCTTTTTCAGAAGCTTTGTTAAATCAAGTGGAGTA
TCCCAGATAGTTTTCACCTTCCTTCTGATTCCATGTTGCTTGACTCTGAATTTCAGAGCA
CCTCCTGTTATTCCAAATGTGCCTTTCCTCTGGGCCTGGAATGCCCCAAGTGAATTTTGT
CTTGGAAAATTTGATGAGCCACTAGATATGAGCCTCTTCTCTTTCATAGGAAGCCCCCGA
ATAAACGCCACCGGGCAAGGTGTTACAATATTTTATGTTGATAGACTTGGCTACTATCCT
TACATAGATTCAATCACAGGAGTAACTGTGAATGGAGGAATCCCCCAGAAGATTTCCTTA
CAAGACCATCTGGACAAAGCTAAGAAAGACATTACATTTTATATGCCAGTAGACAATTTG
GGAATGGCTGTTATTGACTGGGAAGAATGGAGACCCACTTGGGCAAGAAACTGGAAACCT
AAAGATGTTTACAAGAATAGGTCTATTGAATTGGTTCAGCAACAAAATGTACAACTTAGT
CTCACAGAGGCCACTGAGAAAGCAAAACAAGAATTTGAAAAAGCAGGGAAGGATTTCCTG
GTAGAGACTATAAAATTGGGAAAATTACTTCGGCCAAATCACTTGTGGGGTTATTATCTT
TTTCCGGATTGTTACAACCATCACTATAAGAAACCCGGTTACAATGGAAGTTGCTTCAAT
GTAGAAATAAAAAGAAATGATGATCTCAGCTGGTTGTGGAATGAAAGCACTGCTCTTTAC
CCATCCATTTATTTGAACACTCAGCAGTCTCCTGTAGCTGCTACACTCTATGTGCGCAAT
CGAGTTCGGGAAGCCATCAGAGTTTCCAAAATACCTGATGCAAAAAGTCCACTTCCGGTT
TTTGCATATACCCGCATAGTTTTTACTGATCAAGTTTTGAAATTCCTTTCTCAAGATGAA
CTTGTGTATACATTTGGCGAAACTGTTGCTCTGGGTGCTTCTGGAATTGTAATATGGGGA
ACCCTCAGTATAATGCGAAGTATGAAATCTTGCTTGCTCCTAGACAATTACATGGAGACT
ATACTGAATCCTTACATAATCAACGTCACACTAGCAGCCAAAATGTGTAGCCAAGTGCTT
TGCCAGGAGCAAGGAGTGTGTATAAGGAAAAACTGGAATTCAAGTGACTATCTTCACCTC
AACCCAGATAATTTTGCTATTCAACTTGAGAAAGGTGGAAAGTTCACAGTACGTGGAAAA
CCGACACTTGAAGACCTGGAGCAATTTTCTGAAAAATTTTATTGCAGCTGTTATAGCACC
TTGAGTTGTAAGGAGAAAGCTGATGTAAAAGACACTGATGCTGTTGATGTGTGTATTGCT
GATGGTGTCTGTATAGATGCTTTTCTAAAACCTCCCATGGAGACAGAAGAACCTCAAATT
TTCTACAATGCTTCACCCTCCACACTATCTGCCACAATGTTCATTTGGAGGCTGGAAGTC
TGGGATCAAGGTATTAGCAGAATTGGTTTCTTC

ORF Protein

Cloned ORF protein sequence for pFN21AE5612 Download

>KIEE5612  511 aa
MGVLKFKHIFFRSFVKSSGVSQIVFTFLLIPCCLTLNFRAPPVIPNVPFLWAWNAPSEFC
LGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISL
QDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLS
LTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFN
VEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPV
FAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMET
ILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGK
PTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQI
FYNASPSTLSATMFIWRLEVWDQGISRIGFF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5612 Download

>pFN21AE5612  6238 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGAGTGCTAAAATTCAAGCACATCTTTTTCAG
AAGCTTTGTTAAATCAAGTGGAGTATCCCAGATAGTTTTCACCTTCCTTCTGATTCCATG
TTGCTTGACTCTGAATTTCAGAGCACCTCCTGTTATTCCAAATGTGCCTTTCCTCTGGGC
CTGGAATGCCCCAAGTGAATTTTGTCTTGGAAAATTTGATGAGCCACTAGATATGAGCCT
CTTCTCTTTCATAGGAAGCCCCCGAATAAACGCCACCGGGCAAGGTGTTACAATATTTTA
TGTTGATAGACTTGGCTACTATCCTTACATAGATTCAATCACAGGAGTAACTGTGAATGG
AGGAATCCCCCAGAAGATTTCCTTACAAGACCATCTGGACAAAGCTAAGAAAGACATTAC
ATTTTATATGCCAGTAGACAATTTGGGAATGGCTGTTATTGACTGGGAAGAATGGAGACC
CACTTGGGCAAGAAACTGGAAACCTAAAGATGTTTACAAGAATAGGTCTATTGAATTGGT
TCAGCAACAAAATGTACAACTTAGTCTCACAGAGGCCACTGAGAAAGCAAAACAAGAATT
TGAAAAAGCAGGGAAGGATTTCCTGGTAGAGACTATAAAATTGGGAAAATTACTTCGGCC
AAATCACTTGTGGGGTTATTATCTTTTTCCGGATTGTTACAACCATCACTATAAGAAACC
CGGTTACAATGGAAGTTGCTTCAATGTAGAAATAAAAAGAAATGATGATCTCAGCTGGTT
GTGGAATGAAAGCACTGCTCTTTACCCATCCATTTATTTGAACACTCAGCAGTCTCCTGT
AGCTGCTACACTCTATGTGCGCAATCGAGTTCGGGAAGCCATCAGAGTTTCCAAAATACC
TGATGCAAAAAGTCCACTTCCGGTTTTTGCATATACCCGCATAGTTTTTACTGATCAAGT
TTTGAAATTCCTTTCTCAAGATGAACTTGTGTATACATTTGGCGAAACTGTTGCTCTGGG
TGCTTCTGGAATTGTAATATGGGGAACCCTCAGTATAATGCGAAGTATGAAATCTTGCTT
GCTCCTAGACAATTACATGGAGACTATACTGAATCCTTACATAATCAACGTCACACTAGC
AGCCAAAATGTGTAGCCAAGTGCTTTGCCAGGAGCAAGGAGTGTGTATAAGGAAAAACTG
GAATTCAAGTGACTATCTTCACCTCAACCCAGATAATTTTGCTATTCAACTTGAGAAAGG
TGGAAAGTTCACAGTACGTGGAAAACCGACACTTGAAGACCTGGAGCAATTTTCTGAAAA
ATTTTATTGCAGCTGTTATAGCACCTTGAGTTGTAAGGAGAAAGCTGATGTAAAAGACAC
TGATGCTGTTGATGTGTGTATTGCTGATGGTGTCTGTATAGATGCTTTTCTAAAACCTCC
CATGGAGACAGAAGAACCTCAAATTTTCTACAATGCTTCACCCTCCACACTATCTGCCAC
AATGTTCATTTGGAGGCTGGAAGTCTGGGATCAAGGTATTAGCAGAATTGGTTTCTTCGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT