TRIM27 ORF clone FHC08698

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC08698

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Clone info.

Species

human

Clone Name

pFN21AB5957 Sequence (with vector) Restriction Map

Product ID

FHC08698

Flexi Clone PID

FXC08698

ORF Sequence

DNA sequence (1539 bp)
Protein sequence (513 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528391]

Gene

KIBB5957

Symbol

TRIM27

Alias : RFP, RNF76

Description

tripartite motif containing 27

Original Clone ID

cs00657

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1539 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 513 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4654.1 1.2e-153 100.0 TRIM27 CCDS10498.1 2.8e-47 38.6 MEFV CCDS5678.1 4.8e-32 32.9 TRIM4 CCDS4462.1 1.3e-29 40.3 TRIM7 CCDS34375.1 9.2e-28 34.7 TRIM10 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR020457 104 121 PR01406 Zinc finger IPR020457 123 137 PR01406 Zinc finger IPR003879 314 331 PR01407 Butyrophylin-like IPR003879 331 348 PR01407 Butyrophylin-like IPR003879 353 377 PR01407 Butyrophylin-like IPR003879 383 396 PR01407 Butyrophylin-like IPR003879 427 451 PR01407 Butyrophylin-like IPR003879 458 476 PR01407 Butyrophylin-like HMMPfam IPR018957 16 56 PF00097 Zinc finger IPR000315 92 132 PF00643 Zinc finger IPR003877 369 491 PF00622 SPla/RYanodine receptor SPRY HMMSmart IPR001841 16 56 SM00184 Zinc finger IPR000315 91 132 SM00336 Zinc finger IPR006574 315 367 SM00589 SPRY-associated IPR018355 368 493 SM00449 SPla/RYanodine receptor subgroup ProfileScan IPR001841 16 57 PS50089 Zinc finger IPR000315 91 132 PS50119 Zinc finger IPR001870 298 492 PS50188 B302/SPRY domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006501 513 602165 100.0 100.0 NP_000234 781 134610,249100,608107 38.6 93.2 XP_016878725 780 134610,249100,608107 38.6 93.2 NP_976038 511 609315 40.3 92.8 NP_006769 481 605701 34.7 92.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5957 Download

>KIBB5957  1539 bp
ATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCCCGTGTGCCTG
CAGTACTTCGCAGAGCCCATGATGCTCGACTGCGGCCATAACATCTGTTGCGCGTGCCTC
GCCCGCTGCTGGGGCACGGCAGAGACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTC
CCGCAGAGGCACATGCGGCCCAACCGGCACCTGGCCAACGTGACCCAACTGGTAAAGCAG
CTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGAGAAGCACCGC
GAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTGCGTGGTGTGCGACCGC
TCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGCTCGAGGAGGCGGTGGAGGGCTTCAAG
GAGCAAATCCAGAACCAGCTCGACCATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGT
CGGGCCCAGGGGGAACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAG
AAGATTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGAGTATCGCCTC
CTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCATCAATGGTGCCATCACC
CAGTTCTCTTGCAACATCTCCCACCTCAGCAGCCTGATCGCTCAGCTAGAAGAGAAGCAG
CAGCAGCCCACCAGGGAGCTCCTGCAGGACATTGGGGACACATTGAGCAGGGCTGAAAGA
ATCAGGATTCCTGAACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTT
GCCCAAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATGCAGTCA
GATATGGAGAAAATCCAAGAATTAAGAGAGGCTCAGTTATACTCAGTGGACGTGACTCTG
GACCCAGACACGGCCTACCCCAGCCTGATCCTCTCTGATAATCTGCGGCAAGTGCGGTAC
AGTTACCTCCAACAGGACCTGCCTGACAACCCCGAGAGGTTCAATCTGTTTCCCTGTGTC
TTGGGCTCTCCATGCTTCATCGCCGGGAGACATTATTGGGAGGTAGAGGTGGGAGATAAA
GCCAAGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGGAGTAACCTCA
GCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAAAGAATATTGGGCTCTT
ACCTCCCCAATGACTGCCCTACCCCTGCGGACCCCGCTCCAGCGGGTGGGGATTTTCTTG
GACTATGATGCTGGTGAGGTCTCCTTCTACAACGTGACAGAGAGGTGTCACACCTTCACT
TTCTCTCATGCTACCTTTTGTGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGA
GGGAAAAGTGCAGCTCCTCTGATCATCTGCCCCATGAGTGGGATAGATGGGTTTTCTGGC
CATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCT

ORF Protein

Cloned ORF protein sequence for pFN21AB5957 Download

>KIBB5957  513 aa
MASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRETF
PQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDR
SREHRGHSVLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQMERE
KIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQ
QQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQS
DMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCV
LGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWAL
TSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSG
GKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5957 Download

>pFN21AB5957  6244 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCA
GGAGACCACCTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCGG
CCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAGACTAACGTGTC
GTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGGCACCTGGC
CAACGTGACCCAACTGGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGA
GATGGGCGTGTGCGAGAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGAT
GCCCATCTGCGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGCT
CGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGACCATTTAAAAAG
AGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGAACAGGCACGAGCTGAACTCTT
GAGCCTAACCCAGATGGAGAGGGAGAAGATTGTTTGGGAGTTTGAGCAGCTGTATCACTC
CTTAAAGGAGCATGAGTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTA
CAATAGCATCAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCCT
GATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTGCAGGACATTGG
GGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGAACCTTGGATCACACCTCCAGA
TTTGCAAGAGAAAATCCACATTTTTGCCCAAAAATGTCTATTCTTGACGGAGAGTCTAAA
GCAGTTCACAGAAAAAATGCAGTCAGATATGGAGAAAATCCAAGAATTAAGAGAGGCTCA
GTTATACTCAGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTGATCCTCTC
TGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCTGACAACCCCGA
GAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGCTTCATCGCCGGGAGACATTA
TTGGGAGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAAGACTCAGT
GTGCAGAAAAGGTGGAGTAACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTG
GTATGGGAAAGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTGCGGACCCC
GCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTGAGGTCTCCTTCTACAACGT
GACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTACCTTTTGTGGGCCTGTCCGGCC
CTACTTCAGTCTGAGTTACTCGGGAGGGAAAAGTGCAGCTCCTCTGATCATCTGCCCCAT
GAGTGGGATAGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTC
CCCTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT