EIF2A ORF clone FHC08583

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC08583

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Clone info.

Species

human

Clone Name

pFN21AE1363 Sequence (with vector) Restriction Map

Product ID

FHC08583

ORF Sequence

DNA sequence (1755 bp)
Protein sequence (585 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590213]

Gene

KIEE1363

Symbol

EIF2A

Alias : CDA02, EIF-2A, MST089, MSTP004, MSTP089

Description

eukaryotic translation initiation factor 2A, 65kDa

Original Clone ID

oc01363

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1755 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 585 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46935.1 2.2e-175 99.8 EIF2A CCDS82860.1 5.3e-173 98.8 EIF2A CCDS82861.1 6.7e-157 95.6 EIF2A CCDS82859.1 2.5e-125 89.4 EIF2A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013979 216 411 PF08662 Translation initiation factor 2A
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_114414 585 609234 99.8 100.0 NP_001305974 580 609234 98.8 100.0 NP_001305972 560 609234 95.6 100.0 XP_011511526 542 609234 92.5 100.0 NP_001305973 524 609234 89.4 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1363 Download

>KIEE1363  1755 bp
ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAAT
GGACCACCACATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAA
GTCTGTATCTTTAGTAAGGATGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAAT
ATTATCAGTGTCACTAACAAGGGACTACTGCACTCCTTCGACCTCCTGAAGGCAGTTTGC
CTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGCAGCCTTACACTACTTCTAAA
GATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGGGACATGTTTG
AAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT
CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACA
ATTGCAAATAAATTGCATTTGCAAAAAATTAATGACTTTGTATTATCACCTGGACCCCAA
CCATACAAGGTGGCTGTCTATGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTA
TATCAGTACCCCAACTTTGCTGGACCTCATGCAGCTTTAGCTAATAAAAGTTTCTTTAAG
GCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTGCTGTGTTGGTAATAGCTAGC
ACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACACTACATTGCA
ACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT
TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACA
ATTTTCAACTTGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCC
TACTATAGCCCTCATGGACATATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAA
ATGGAAGTGTGGGATGTGAAAAACTACAAACTTATTTCTAAACCGGTGGCTTCTGATTCT
ACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAACAGCTACATGTGCTCCCAGG
TTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTTGCACAAGTAT
GATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA
TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCT
AAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAA
TTGCATGAAGAGGAACCACCTCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTA
TCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGAAGCTAAGAAAGCTGCAAAGCAGGAA
GCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTGCCCCACAGAGCACACCACGA
AACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAATCAAGAACCTA
AAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA
GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGAT
TTGAAATTGGGTATT

ORF Protein

Cloned ORF protein sequence for pFN21AE1363 Download

>KIEE1363  585 aa
MAPSTPLLTVRGSEGLYMVNGPPHFTESTVFPRESGKNCKVCIFSKDGTLFAWGNGEKVN
IISVTNKGLLHSFDLLKAVCLEFSPKNTVLATWQPYTTSKDGTAGIPNLQLYDVKTGTCL
KSFIQKKMQNWCPSWSEDETLCARNVNNEVHFFENNNFNTIANKLHLQKINDFVLSPGPQ
PYKVAVYVPGSKGAPSFVRLYQYPNFAGPHAALANKSFFKADKVTMLWNKKATAVLVIAS
TDVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVVWNSSSTEFCAVYGFMPAKAT
IFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGFGNLRGQMEVWDVKNYKLISKPVASDS
TYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSILHKYDVPSNAELWQVSWQPFLDGI
FPAKTITYQAVPSEVPNEEPKVATAYRPPALRNKPITNSKLHEEEPPQNMKPQSGNDKPL
SKTALKNQRKHEAKKAAKQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNL
KKKLKAIEQLKEQAATGKQLEKNQLEKIQKETALLQELEDLKLGI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1363 Download

>pFN21AE1363  6460 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGG
ATCAGAAGGACTGTACATGGTGAATGGACCACCACATTTTACAGAAAGCACAGTGTTTCC
AAGGGAATCTGGGAAGAATTGCAAAGTCTGTATCTTTAGTAAGGATGGGACCTTGTTTGC
CTGGGGCAATGGAGAAAAAGTAAATATTATCAGTGTCACTAACAAGGGACTACTGCACTC
CTTCGACCTCCTGAAGGCAGTTTGCCTTGAATTCTCACCCAAAAATACTGTCCTGGCAAC
GTGGCAGCCTTACACTACTTCTAAAGATGGCACAGCTGGGATACCCAACCTACAACTTTA
TGATGTGAAAACTGGGACATGTTTGAAATCTTTCATCCAGAAAAAAATGCAAAATTGGTG
TCCATCCTGGTCAGAAGATGAAACTCTTTGTGCCCGCAATGTTAACAATGAAGTTCACTT
CTTTGAAAACAACAATTTTAACACAATTGCAAATAAATTGCATTTGCAAAAAATTAATGA
CTTTGTATTATCACCTGGACCCCAACCATACAAGGTGGCTGTCTATGTTCCAGGAAGTAA
AGGTGCACCTTCATTTGTTAGATTATATCAGTACCCCAACTTTGCTGGACCTCATGCAGC
TTTAGCTAATAAAAGTTTCTTTAAGGCAGATAAAGTTACAATGCTGTGGAATAAAAAAGC
TACTGCTGTGTTGGTAATAGCTAGCACAGATGTTGACAAGACAGGAGCTTCCTACTATGG
AGAACAAACTCTACACTACATTGCAACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAA
AAATGGCCCCATTTATGATGTAGTTTGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATA
TGGTTTTATGCCTGCCAAAGCGACAATTTTCAACTTGAAATGTGATCCTGTATTTGACTT
TGGAACTGGTCCTCGTAATGCAGCCTACTATAGCCCTCATGGACATATATTAGTATTAGC
TGGATTTGGAAATCTGAGGGGACAAATGGAAGTGTGGGATGTGAAAAACTACAAACTTAT
TTCTAAACCGGTGGCTTCTGATTCTACATATTTTGCTTGGTGCCCGGATGGTGAGCATAT
TTTAACAGCTACATGTGCTCCCAGGTTACGGGTTAATAATGGATACAAAATTTGGCATTA
TACTGGCTCTATCTTGCACAAGTATGATGTGCCATCAAATGCAGAATTATGGCAGGTTTC
TTGGCAGCCATTTTTGGATGGAATATTTCCAGCAAAAACAATAACTTACCAAGCAGTTCC
AAGTGAAGTACCCAATGAGGAACCTAAAGTTGCAACAGCTTATAGACCCCCAGCTTTAAG
AAATAAACCAATCACCAATTCCAAATTGCATGAAGAGGAACCACCTCAGAATATGAAACC
ACAATCAGGAAACGATAAGCCATTATCAAAAACAGCTCTTAAAAATCAAAGGAAGCATGA
AGCTAAGAAAGCTGCAAAGCAGGAAGCAAGAAGTGACAAGAGTCCAGATTTGGCACCTAC
TCCTGCCCCACAGAGCACACCACGAAACACTGTCTCTCAGTCAATTTCTGGGGACCCTGA
GATAGACAAAAAAATCAAGAACCTAAAGAAGAAACTGAAAGCAATCGAACAACTGAAAGA
ACAAGCAGCAACTGGAAAACAGCTAGAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAAC
AGCCCTTCTCCAGGAGCTGGAAGATTTGAAATTGGGTATTGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT