ZNF454 ORF clone FHC07478

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Halotag ORF Clone FHC07478

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Clone info.

Species

human

Clone Name

pFN21AB7805 Sequence (with vector) Restriction Map

Product ID

FHC07478

Flexi Clone PID

FXC07478

ORF Sequence

DNA sequence (1566 bp)
Protein sequence (522 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464401]

Gene

KIBB7805

Symbol

ZNF454

Description

zinc finger protein 454, transcript variant 2

Original Clone ID

cs00305

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1566 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 522 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4441.1 2.8e-121 99.8 ZNF454 CCDS12504.1 7.1e-57 50.0 ZNF570 CCDS74355.1 7.5e-57 50.0 ZNF570 CCDS42982.1 1.2e-51 46.8 ZNF74 CCDS75075.1 1.4e-49 49.4 ZNF595 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001909 14 54 PF01352 Krueppel-associated box IPR007087 178 200 PF00096 Zinc finger IPR007087 244 266 PF00096 Zinc finger IPR007087 328 350 PF00096 Zinc finger IPR007087 356 378 PF00096 Zinc finger IPR007087 384 406 PF00096 Zinc finger IPR007087 412 434 PF00096 Zinc finger IPR007087 440 462 PF00096 Zinc finger IPR007087 468 490 PF00096 Zinc finger IPR007087 496 518 PF00096 Zinc finger HMMSmart IPR001909 14 73 SM00349 Krueppel-associated box IPR015880 178 200 SM00355 Zinc finger IPR015880 216 238 SM00355 Zinc finger IPR015880 244 266 SM00355 Zinc finger IPR015880 272 294 SM00355 Zinc finger IPR015880 300 322 SM00355 Zinc finger IPR015880 328 350 SM00355 Zinc finger IPR015880 356 378 SM00355 Zinc finger IPR015880 384 406 SM00355 Zinc finger IPR015880 412 434 SM00355 Zinc finger IPR015880 440 462 SM00355 Zinc finger IPR015880 468 490 SM00355 Zinc finger IPR015880 496 518 SM00355 Zinc finger ProfileScan IPR001909 14 84 PS50805 Krueppel-associated box IPR007087 178 205 PS50157 Zinc finger IPR007087 216 243 PS50157 Zinc finger IPR007087 244 271 PS50157 Zinc finger IPR007087 272 299 PS50157 Zinc finger IPR007087 300 327 PS50157 Zinc finger IPR007087 328 355 PS50157 Zinc finger IPR007087 356 383 PS50157 Zinc finger IPR007087 384 411 PS50157 Zinc finger IPR007087 412 439 PS50157 Zinc finger IPR007087 440 467 PS50157 Zinc finger IPR007087 468 495 PS50157 Zinc finger IPR007087 496 522 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001243453 644 194548 46.8 97.3 NP_003417 644 194548 46.8 97.3 NP_115799 628 615580 45.9 98.5 NP_001152765 536 603984 48.7 97.1 XP_006722660 550 603984 48.7 97.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7805 Download

>KIBB7805  1566 bp
ATGGCTGTCAGCCACCTGCCAACCATGGTCCAGGAATCGGTGACCTTCAAGGATGTGGCT
ATACTGTTCACCCAGGAAGAGTGGGGGCAGCTGAGCCCCGCCCAGAGGGCCCTGTACAGG
GACGTGATGCTGGAGAACTACAGCAACCTGGTCTCACTGGGACTCTTAGGACCCAAACCA
GATACGTTTTCCCAGCTAGAAAAAAGGGAAGTGTGGATGCCAGAGGACACCCCTGGAGGC
TTCTGTCTTGACTGGATGACTATGCCTGCCAGTAAGAAATCTACTGTCAAGGCAGAGATT
CCTGAAGAAGAATTGGATCAATGGACAATAAAGGAAAGATTCAGTAGCAGTAGTCACTGG
AAGTGTGCTAGCCTGCTGGAGTGGCAATGTGGAGGCCAGGAGATCAGTTTGCAGCGAGTG
GTACTCACTCACCCCAACACCCCATCACAGGAATATGATGAATCCGGGAGCACTATGAGC
TCATCTCTTCACAGTGATCAAAGTCAGGGATTTCAACCTAGCAAAAATGCCTTTGAGTGT
AGTGAGTGTGGAAAAGTCTTCTCTAAGAGTTCAACTCTTAATAAACATCAGAAAATTCAT
AATGAAAAAAATGCAAATCAGAAAATTCATATTAAGGAGAAAAGATATGAATGTAGAGAA
TGTGGGAAAGCCTTTCACCAGAGTACGCACCTTATCCATCACCAAAGAATTCACACTGGC
GAGAAACCCTATGAATGTAAGGAATGTGGCAAGGCCTTCTCAGTGAGCTCCTCACTTACG
TACCATCAGAAAATTCATACTGGAGAGAAGCCTTTTGAATGCAACTTATGTGGAAAAGCT
TTTATCCGAAATATACACCTTGCCCATCATCATAGAATACATACTGGAGAGAAACCTTTT
AAATGTAACATTTGTGAAAAAGCCTTTGTGTGCAGGGCACACCTTACCAAACACCAGAAT
ATCCACAGTGGAGAGAAACCCTATAAATGCAATGAATGTGGAAAAGCCTTTAATCAGAGT
ACAAGTTTCCTTCAGCATCAGAGAATTCACACTGGAGAGAAACCCTTTGAATGTAATGAA
TGTGGGAAGGCCTTCAGGGTGAACTCTTCCCTTACTGAACATCAGAGAATTCATACTGGA
GAGAAACCTTATAAATGTAATGAATGTGGGAAAGCTTTCAGGGATAATTCATCCTTTGCA
CGACATCGGAAAATTCACACTGGAGAGAAACCTTACAGATGTGGCTTGTGTGAGAAAGCC
TTTCGGGACCAATCAGCACTAGCCCAACATCAGAGAATTCATACTGGGGAAAAACCTTAT
ACATGTAACATATGTGAAAAAGCCTTCAGTGACCATTCAGCCCTTACCCAACATAAGAGA
ATTCATACTAGGGAAAAACCTTACAAATGTAAAATCTGTGAGAAAGCCTTTATCCGAAGC
ACTCACCTGACTCAACATCAGAGGATTCACACAGGAGAGAAACCCTATAAATGTAATAAA
TGTGGGAAAGCTTTTAACCAGACTGCAAACCTCATTCAGCATCAGAGACATCATATTGGA
GAGAAG

ORF Protein

Cloned ORF protein sequence for pFN21AB7805 Download

>KIBB7805  522 aa
MAVSHLPTMVQESVTFKDVAILFTQEEWGQLSPAQRALYRDVMLENYSNLVSLGLLGPKP
DTFSQLEKREVWMPEDTPGGFCLDWMTMPASKKSTVKAEIPEEELDQWTIKERFSSSSHW
KCASLLEWQCGGQEISLQRVVLTHPNTPSQEYDESGSTMSSSLHSDQSQGFQPSKNAFEC
SECGKVFSKSSTLNKHQKIHNEKNANQKIHIKEKRYECRECGKAFHQSTHLIHHQRIHTG
EKPYECKECGKAFSVSSSLTYHQKIHTGEKPFECNLCGKAFIRNIHLAHHHRIHTGEKPF
KCNICEKAFVCRAHLTKHQNIHSGEKPYKCNECGKAFNQSTSFLQHQRIHTGEKPFECNE
CGKAFRVNSSLTEHQRIHTGEKPYKCNECGKAFRDNSSFARHRKIHTGEKPYRCGLCEKA
FRDQSALAQHQRIHTGEKPYTCNICEKAFSDHSALTQHKRIHTREKPYKCKICEKAFIRS
THLTQHQRIHTGEKPYKCNKCGKAFNQTANLIQHQRHHIGEK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7805 Download

>pFN21AB7805  6271 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGTCAGCCACCTGCCAACCATGGTCCAGGA
ATCGGTGACCTTCAAGGATGTGGCTATACTGTTCACCCAGGAAGAGTGGGGGCAGCTGAG
CCCCGCCCAGAGGGCCCTGTACAGGGACGTGATGCTGGAGAACTACAGCAACCTGGTCTC
ACTGGGACTCTTAGGACCCAAACCAGATACGTTTTCCCAGCTAGAAAAAAGGGAAGTGTG
GATGCCAGAGGACACCCCTGGAGGCTTCTGTCTTGACTGGATGACTATGCCTGCCAGTAA
GAAATCTACTGTCAAGGCAGAGATTCCTGAAGAAGAATTGGATCAATGGACAATAAAGGA
AAGATTCAGTAGCAGTAGTCACTGGAAGTGTGCTAGCCTGCTGGAGTGGCAATGTGGAGG
CCAGGAGATCAGTTTGCAGCGAGTGGTACTCACTCACCCCAACACCCCATCACAGGAATA
TGATGAATCCGGGAGCACTATGAGCTCATCTCTTCACAGTGATCAAAGTCAGGGATTTCA
ACCTAGCAAAAATGCCTTTGAGTGTAGTGAGTGTGGAAAAGTCTTCTCTAAGAGTTCAAC
TCTTAATAAACATCAGAAAATTCATAATGAAAAAAATGCAAATCAGAAAATTCATATTAA
GGAGAAAAGATATGAATGTAGAGAATGTGGGAAAGCCTTTCACCAGAGTACGCACCTTAT
CCATCACCAAAGAATTCACACTGGCGAGAAACCCTATGAATGTAAGGAATGTGGCAAGGC
CTTCTCAGTGAGCTCCTCACTTACGTACCATCAGAAAATTCATACTGGAGAGAAGCCTTT
TGAATGCAACTTATGTGGAAAAGCTTTTATCCGAAATATACACCTTGCCCATCATCATAG
AATACATACTGGAGAGAAACCTTTTAAATGTAACATTTGTGAAAAAGCCTTTGTGTGCAG
GGCACACCTTACCAAACACCAGAATATCCACAGTGGAGAGAAACCCTATAAATGCAATGA
ATGTGGAAAAGCCTTTAATCAGAGTACAAGTTTCCTTCAGCATCAGAGAATTCACACTGG
AGAGAAACCCTTTGAATGTAATGAATGTGGGAAGGCCTTCAGGGTGAACTCTTCCCTTAC
TGAACATCAGAGAATTCATACTGGAGAGAAACCTTATAAATGTAATGAATGTGGGAAAGC
TTTCAGGGATAATTCATCCTTTGCACGACATCGGAAAATTCACACTGGAGAGAAACCTTA
CAGATGTGGCTTGTGTGAGAAAGCCTTTCGGGACCAATCAGCACTAGCCCAACATCAGAG
AATTCATACTGGGGAAAAACCTTATACATGTAACATATGTGAAAAAGCCTTCAGTGACCA
TTCAGCCCTTACCCAACATAAGAGAATTCATACTAGGGAAAAACCTTACAAATGTAAAAT
CTGTGAGAAAGCCTTTATCCGAAGCACTCACCTGACTCAACATCAGAGGATTCACACAGG
AGAGAAACCCTATAAATGTAATAAATGTGGGAAAGCTTTTAACCAGACTGCAAACCTCAT
TCAGCATCAGAGACATCATATTGGAGAGAAGGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT