ASPHD2 ORF clone FHC04193

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC04193

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Clone info.

Species

human

Clone Name

pFN21AE0488 Sequence (with vector) Restriction Map

Product ID

FHC04193

Flexi Clone PID

FXC04193

ORF Sequence

DNA sequence (1107 bp)
Protein sequence (369 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528069]

Gene

KIEE0488

Symbol

ASPHD2

Description

aspartate beta-hydroxylase domain containing 2

Original Clone ID

oc01188

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1107 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 369 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13834.2 5.8e-170 100.0 ASPHD2 CCDS10660.1 5.3e-60 49.8 ASPHD1 CCDS55234.1 7.3e-18 30.1 ASPH CCDS34898.1 7.6e-18 30.1 ASPH The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007803 191 351 PF05118 Aspartyl/Asparaginyl beta-hydroxylase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 25 PKMSLEWLVAWSWSLDGLRDCIA 47 SECONDARY 23 2 56 CDTTAVITVACLLVLFVWYCYHV 78 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0488 Download

>KIEE0488  1107 bp
ATGGTGTGGGCGCCCTTGGGACCCCCGAGGACTGATTGTCTGACCTTGCTTCACACGCCC
AGTAAGGACTCCCCCAAGATGTCGCTCGAGTGGCTGGTGGCCTGGAGCTGGTCGCTGGAT
GGCCTGAGGGACTGCATCGCCACCGGCATCCAGTCCGTGCGGGACTGCGACACCACCGCT
GTCATCACTGTGGCCTGCCTCCTGGTCCTCTTCGTGTGGTACTGTTATCACGTGGGCAGG
GAGCAGCCCCGGCCCTACGTCTCCGTCAACTCCCTCATGCAGGCTGCCGATGCCAACGGG
CTGCAGAATGGCTACGTGTACTGCCAGTCCCCTGAGTGCGTGCGCTGCACCCACAACGAG
GGCCTCAACCAGAAGCTGTACCACAACCTGCAGGAGTACGCCAAGCGCTACTCCTGGTCC
GGCATGGGCCGCATCCACAAGGGCATCCGCGAGCAGGGCCGGTACCTCAACAGCCGGCCC
TCCATCCAGAAGCCCGAGGTCTTCTTCCTGCCCGACCTGCCCACCACGCCCTATTTCTCC
CGGGACGCACAGAAACATGATGTGGAAGTGCTGGAACGGAACTTCCAGACCATCCTGTGT
GAGTTTGAGACCCTCTACAAAGCTTTCTCAAACTGCAGCCTCCCGCAAGGATGGAAAATG
AACAGCACCCCCAGCGGGGAGTGGTTCACCTTTTACTTGGTCAATCAGGGGGTTTGTGTT
CCCAGGAACTGTAGGAAGTGCCCACGGACGTACCGCTTGCTCGGAAGCCTTCGGACCTGT
ATTGGGAACAATGTTTTTGGGAACGCGTGCATCTCTGTGCTGAGCCCTGGGACTGTGATA
ACGGAGCACTATGGACCCACCAACATCCGCATCCGATGCCATTTAGGTCTGAAAACTCCA
AATGGCTGTGAGCTGGTGGTGGGGGGAGAGCCCCAGTGCTGGGCAGAAGGGCGCTGCCTT
CTCTTTGATGACTCTTTCCTGCATGCTGCGTTCCATGAAGGTTCAGCAGAGGATGGCCCA
CGGGTGGTTTTCATGGTGGATTTGTGGCATCCAAACGTCGCAGCGGCCGAACGGCAGGCT
CTTGATTTCATCTTTGCTCCGGGACGA

ORF Protein

Cloned ORF protein sequence for pFN21AE0488 Download

>KIEE0488  369 aa
MVWAPLGPPRTDCLTLLHTPSKDSPKMSLEWLVAWSWSLDGLRDCIATGIQSVRDCDTTA
VITVACLLVLFVWYCYHVGREQPRPYVSVNSLMQAADANGLQNGYVYCQSPECVRCTHNE
GLNQKLYHNLQEYAKRYSWSGMGRIHKGIREQGRYLNSRPSIQKPEVFFLPDLPTTPYFS
RDAQKHDVEVLERNFQTILCEFETLYKAFSNCSLPQGWKMNSTPSGEWFTFYLVNQGVCV
PRNCRKCPRTYRLLGSLRTCIGNNVFGNACISVLSPGTVITEHYGPTNIRIRCHLGLKTP
NGCELVVGGEPQCWAEGRCLLFDDSFLHAAFHEGSAEDGPRVVFMVDLWHPNVAAAERQA
LDFIFAPGR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0488 Download

>pFN21AE0488  5812 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTGTGGGCGCCCTTGGGACCCCCGAGGACTGA
TTGTCTGACCTTGCTTCACACGCCCAGTAAGGACTCCCCCAAGATGTCGCTCGAGTGGCT
GGTGGCCTGGAGCTGGTCGCTGGATGGCCTGAGGGACTGCATCGCCACCGGCATCCAGTC
CGTGCGGGACTGCGACACCACCGCTGTCATCACTGTGGCCTGCCTCCTGGTCCTCTTCGT
GTGGTACTGTTATCACGTGGGCAGGGAGCAGCCCCGGCCCTACGTCTCCGTCAACTCCCT
CATGCAGGCTGCCGATGCCAACGGGCTGCAGAATGGCTACGTGTACTGCCAGTCCCCTGA
GTGCGTGCGCTGCACCCACAACGAGGGCCTCAACCAGAAGCTGTACCACAACCTGCAGGA
GTACGCCAAGCGCTACTCCTGGTCCGGCATGGGCCGCATCCACAAGGGCATCCGCGAGCA
GGGCCGGTACCTCAACAGCCGGCCCTCCATCCAGAAGCCCGAGGTCTTCTTCCTGCCCGA
CCTGCCCACCACGCCCTATTTCTCCCGGGACGCACAGAAACATGATGTGGAAGTGCTGGA
ACGGAACTTCCAGACCATCCTGTGTGAGTTTGAGACCCTCTACAAAGCTTTCTCAAACTG
CAGCCTCCCGCAAGGATGGAAAATGAACAGCACCCCCAGCGGGGAGTGGTTCACCTTTTA
CTTGGTCAATCAGGGGGTTTGTGTTCCCAGGAACTGTAGGAAGTGCCCACGGACGTACCG
CTTGCTCGGAAGCCTTCGGACCTGTATTGGGAACAATGTTTTTGGGAACGCGTGCATCTC
TGTGCTGAGCCCTGGGACTGTGATAACGGAGCACTATGGACCCACCAACATCCGCATCCG
ATGCCATTTAGGTCTGAAAACTCCAAATGGCTGTGAGCTGGTGGTGGGGGGAGAGCCCCA
GTGCTGGGCAGAAGGGCGCTGCCTTCTCTTTGATGACTCTTTCCTGCATGCTGCGTTCCA
TGAAGGTTCAGCAGAGGATGGCCCACGGGTGGTTTTCATGGTGGATTTGTGGCATCCAAA
CGTCGCAGCGGCCGAACGGCAGGCTCTTGATTTCATCTTTGCTCCGGGACGAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT