DDX6 ORF clone FHC03427

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03427

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Clone info.

Species

human

Clone Name

pFN21AB5842 Sequence (with vector) Restriction Map

Product ID

FHC03427

Flexi Clone PID

FXC03427

ORF Sequence

DNA sequence (1449 bp)
Protein sequence (483 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527511]

Gene

KIBB5842

Symbol

DDX6

Alias : HLR2, IDDILF, P54, RCK, Rck/p54

Description

DEAD (Asp-Glu-Ala-Asp) box helicase 6, transcript variant 1

Original Clone ID

cs00542

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1449 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 483 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS44751.1 1e-196 100.0 DDX6 CCDS11767.1 1.4e-54 36.8 EIF4A3 CCDS11113.1 2.9e-54 38.7 EIF4A1 CCDS3282.1 2e-53 38.4 EIF4A2 CCDS12308.1 1.6e-47 36.0 DDX39A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 121 286 PF00270 DNA/RNA helicase IPR001650 353 429 PF00271 Helicase HMMSmart IPR014001 115 312 SM00487 DEAD-like helicase IPR001650 348 429 SM00490 Helicase ProfileScan IPR014014 96 124 PS51195 RNA helicase IPR014001 127 298 PS51192 DEAD-like helicase IPR001650 308 468 PS51194 Helicase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004388 483 600326 100.0 100.0 NP_001244120 483 600326 100.0 100.0 XP_005271474 483 600326 100.0 100.0 XP_016872740 440 600326 91.1 100.0 XP_011540946 440 600326 91.1 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5842 Download

>KIBB5842  1449 bp
ATGAGCACGGCCAGAACAGAGAACCCTGTTATAATGGGTCTGTCCAGTCAAAATGGTCAG
CTGAGAGGCCCTGTGAAACCCACTGGTGGCCCTGGAGGAGGGGGCACACAGACACAGCAA
CAGATGAACCAGCTGAAAAACACCAACACAATCAATAATGGCACTCAGCAGCAAGCACAG
AGTATGACCACCACTATTAAACCTGGTGATGACTGGAAAAAGACTTTAAAACTCCCTCCA
AAGGATCTAAGAATCAAAACTTCGGATGTGACCTCCACAAAAGGAAATGAGTTTGAAGAT
TACTGTTTGAAACGGGAGTTACTGATGGGAATTTTTGAAATGGGCTGGGAAAAGCCATCT
CCTATTCAGGAGGAGAGCATTCCCATTGCTTTATCTGGTAGGGATATCTTAGCTAGAGCA
AAAAATGGAACAGGCAAGAGCGGTGCCTACCTCATTCCCTTACTTGAACGGCTAGACCTG
AAGAAGGACAATATACAAGCAATGGTGATTGTTCCCACTAGAGAACTTGCTCTACAGGTC
AGTCAAATTTGCATCCAGGTCAGCAAACACATGGGAGGGGCCAAAGTGATGGCAACCACA
GGAGGAACCAATTTACGAGATGACATAATGAGGCTTGATGATACAGTGCACGTGGTGATT
GCTACCCCTGGGAGAATCCTGGATCTTATTAAGAAAGGAGTAGCAAAGGTTGATCATGTC
CAGATGATAGTATTGGATGAGGCAGATAAGTTGCTGTCACAGGATTTTGTGCAGATAATG
GAGGATATTATTCTCACGCTACCTAAAAACAGGCAGATTTTACTATATTCCGCTACTTTC
CCTCTTAGTGTACAGAAGTTCATGAATTCCCATTTGCAGAAACCCTATGAGATTAACCTG
ATGGAGGAACTAACTCTGAAGGGAGTAACCCAGTACTACGCATATGTAACTGAGCGCCAA
AAAGTACACTGCCTCAACACACTTTTCTCCAGGCTTCAGATAAACCAGTCGATCATTTTC
TGTAACTCCTCTCAGCGAGTTGAATTGCTAGCCAAGAAGATTTCTCAACTGGGTTATTCT
TGCTTCTATATTCATGCTAAAATGAGGCAGGAACATCGAAATCGTGTATTTCATGATTTC
CGAAATGGCTTATGCCGCAATCTTGTTTGCACTGATCTGTTTACCCGAGGTATTGATATA
CAAGCTGTGAATGTGGTAATAAACTTTGATTTCCCAAAGCTGGCAGAGACCTATCTCCAT
CGTATTGGAAGATCAGGTCGCTTTGGTCATCTTGGCTTAGCCATCAACTTGATCACATAT
GATGATCGCTTCAACCTGAAAAGTATTGAGGAGCAGCTGGGAACAGAAATTAAACCTATT
CCGAGCAACATTGATAAGAGCCTGTATGTGGCAGAATACCACAGCGAGCCTGTAGAAGAT
GAGAAACCT

ORF Protein

Cloned ORF protein sequence for pFN21AB5842 Download

>KIBB5842  483 aa
MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQ
SMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPS
PIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQV
SQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHV
QMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINL
MEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYS
CFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLH
RIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVED
EKP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5842 Download

>pFN21AB5842  6154 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCACGGCCAGAACAGAGAACCCTGTTATAAT
GGGTCTGTCCAGTCAAAATGGTCAGCTGAGAGGCCCTGTGAAACCCACTGGTGGCCCTGG
AGGAGGGGGCACACAGACACAGCAACAGATGAACCAGCTGAAAAACACCAACACAATCAA
TAATGGCACTCAGCAGCAAGCACAGAGTATGACCACCACTATTAAACCTGGTGATGACTG
GAAAAAGACTTTAAAACTCCCTCCAAAGGATCTAAGAATCAAAACTTCGGATGTGACCTC
CACAAAAGGAAATGAGTTTGAAGATTACTGTTTGAAACGGGAGTTACTGATGGGAATTTT
TGAAATGGGCTGGGAAAAGCCATCTCCTATTCAGGAGGAGAGCATTCCCATTGCTTTATC
TGGTAGGGATATCTTAGCTAGAGCAAAAAATGGAACAGGCAAGAGCGGTGCCTACCTCAT
TCCCTTACTTGAACGGCTAGACCTGAAGAAGGACAATATACAAGCAATGGTGATTGTTCC
CACTAGAGAACTTGCTCTACAGGTCAGTCAAATTTGCATCCAGGTCAGCAAACACATGGG
AGGGGCCAAAGTGATGGCAACCACAGGAGGAACCAATTTACGAGATGACATAATGAGGCT
TGATGATACAGTGCACGTGGTGATTGCTACCCCTGGGAGAATCCTGGATCTTATTAAGAA
AGGAGTAGCAAAGGTTGATCATGTCCAGATGATAGTATTGGATGAGGCAGATAAGTTGCT
GTCACAGGATTTTGTGCAGATAATGGAGGATATTATTCTCACGCTACCTAAAAACAGGCA
GATTTTACTATATTCCGCTACTTTCCCTCTTAGTGTACAGAAGTTCATGAATTCCCATTT
GCAGAAACCCTATGAGATTAACCTGATGGAGGAACTAACTCTGAAGGGAGTAACCCAGTA
CTACGCATATGTAACTGAGCGCCAAAAAGTACACTGCCTCAACACACTTTTCTCCAGGCT
TCAGATAAACCAGTCGATCATTTTCTGTAACTCCTCTCAGCGAGTTGAATTGCTAGCCAA
GAAGATTTCTCAACTGGGTTATTCTTGCTTCTATATTCATGCTAAAATGAGGCAGGAACA
TCGAAATCGTGTATTTCATGATTTCCGAAATGGCTTATGCCGCAATCTTGTTTGCACTGA
TCTGTTTACCCGAGGTATTGATATACAAGCTGTGAATGTGGTAATAAACTTTGATTTCCC
AAAGCTGGCAGAGACCTATCTCCATCGTATTGGAAGATCAGGTCGCTTTGGTCATCTTGG
CTTAGCCATCAACTTGATCACATATGATGATCGCTTCAACCTGAAAAGTATTGAGGAGCA
GCTGGGAACAGAAATTAAACCTATTCCGAGCAACATTGATAAGAGCCTGTATGTGGCAGA
ATACCACAGCGAGCCTGTAGAAGATGAGAAACCTGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT