ZNF174 ORF clone FHC03414

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03414

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Clone info.

Species

human

Clone Name

pFN21AB7968 Sequence (with vector) Restriction Map

Product ID

FHC03414

Flexi Clone PID

FXC03414

ORF Sequence

DNA sequence (1221 bp)
Protein sequence (407 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527498]

Gene

KIBB7968

Symbol

ZNF174

Alias : ZSCAN8

Description

zinc finger protein 174, transcript variant 1

Original Clone ID

cs00468

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1221 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 407 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10504.1 1.9e-100 100.0 ZNF174 CCDS32380.1 7.2e-48 97.2 ZNF174 CCDS76814.1 4.2e-25 37.1 ZNF500 CCDS32383.1 4.4e-25 37.0 ZNF500 CCDS42427.1 9.6e-23 36.2 ZSCAN30 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003309 41 135 PF02023 Transcription regulator SCAN IPR007087 326 348 PF00096 Zinc finger IPR007087 354 376 PF00096 Zinc finger IPR007087 382 404 PF00096 Zinc finger HMMSmart IPR003309 42 154 SM00431 Transcription regulator SCAN IPR015880 326 348 SM00355 Zinc finger IPR015880 354 376 SM00355 Zinc finger IPR015880 382 404 SM00355 Zinc finger ProfileScan IPR003309 46 126 PS50804 Transcription regulator SCAN IPR007087 326 353 PS50157 Zinc finger IPR007087 354 381 PS50157 Zinc finger IPR007087 382 407 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003441 407 603900 100.0 100.0 XP_011513319 565 603978 36.8 96.8 XP_011525219 491 165260 38.4 89.7 NP_001308045 491 165260 38.4 89.7 NP_862829 491 165260 38.4 89.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7968 Download

>KIBB7968  1221 bp
ATGGCAGCTAAAATGGAGATAACTTTAAGCTCCAACACTGAAGCTTCCTCCAAGCAAGAG
AGACACATAATAGCCAAACTAGAAGAGAAACGGGGCCCTCCTCTGCAAAAAAACTGCCCA
GATCCTGAGCTCTGCCGCCAGAGCTTCAGACGCTTTTGTTATCAAGAGGTGTCTGGACCC
CAAGAGGCGCTCTCCCAGCTCCGACAGCTCTGCCGTCAGTGGTTGCAACCCGAGCTGCAC
ACCAAGGAGCAGATTTTGGAGCTTCTGGTGATGGAGCAGTTCCTGACCATCCTGCCCCCG
GAGATCCAGGCTCGGGTCAGGCATCGATGTCCAATGAGCAGCAAGGAGATTGTGACCCTC
GTGGAAGATTTTCACAGAGCATCCAAGAAACCAAAGCAGTGGGTGGCCGTTTGTATGCAG
GGGCAAAAGGTGCTCTTGGAGAAAACTGGATCTCAGCTTGGAGAACAGGAACTGCCAGAC
TTTCAACCGCAGACTCCTAGGAGAGATCTCAGGGAGAGCTCTCCAGCAGAGCCTTCCCAG
GCAGGAGCTTATGACCGGCTGAGCCCCCATCATTGGGAGAAATCCCCACTCCTCCAAGAA
CCAACCCCCAAATTGGCTGGGACAGAGGCCCCCAGAATGAGAAGTGACAACAAGGAAAAT
CCACAACAGGAAGGGGCTAAAGGAGCAAAGCCATGTGCAGTGTCAGCTGGCAGATCCAAA
GGGAATGGTCTGCAGAATCCTGAACCAAGAGGGGCAAATATGAGTGAACCTCGGTTGTCA
CGGAGGCAGGTCAGCTCCCCAAATGCTCAAAAGCCATTTGCTCACTACCAGAGACATTGC
AGGGTGGAATACATCAGCAGCCCCCTAAAAAGCCACCCACTGAGAGAGCTAAAGAAAAGC
AAAGGAGGTAAACGGAGTCTGAGCAACCGTTTGCAACATCTTGGTCACCAGCCCACCCGC
TCAGCAAAGAAACCCTACAAATGTGATGACTGTGGGAAAAGCTTCACGTGGAATTCAGAG
CTGAAGAGACACAAGAGAGTCCACACAGGAGAGAGACCCTACACGTGCGGAGAGTGTGGA
AACTGCTTTGGGCGGCAGTCAACCCTGAAGCTGCACCAGAGGATCCACACTGGAGAGAAG
CCATACCAGTGTGGCCAGTGTGGGAAAAGCTTTCGCCAGAGCTCAAACCTTCACCAGCAT
CACCGACTTCACCATGGGGAC

ORF Protein

Cloned ORF protein sequence for pFN21AB7968 Download

>KIBB7968  407 aa
MAAKMEITLSSNTEASSKQERHIIAKLEEKRGPPLQKNCPDPELCRQSFRRFCYQEVSGP
QEALSQLRQLCRQWLQPELHTKEQILELLVMEQFLTILPPEIQARVRHRCPMSSKEIVTL
VEDFHRASKKPKQWVAVCMQGQKVLLEKTGSQLGEQELPDFQPQTPRRDLRESSPAEPSQ
AGAYDRLSPHHWEKSPLLQEPTPKLAGTEAPRMRSDNKENPQQEGAKGAKPCAVSAGRSK
GNGLQNPEPRGANMSEPRLSRRQVSSPNAQKPFAHYQRHCRVEYISSPLKSHPLRELKKS
KGGKRSLSNRLQHLGHQPTRSAKKPYKCDDCGKSFTWNSELKRHKRVHTGERPYTCGECG
NCFGRQSTLKLHQRIHTGEKPYQCGQCGKSFRQSSNLHQHHRLHHGD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7968 Download

>pFN21AB7968  5926 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGCTAAAATGGAGATAACTTTAAGCTCCAA
CACTGAAGCTTCCTCCAAGCAAGAGAGACACATAATAGCCAAACTAGAAGAGAAACGGGG
CCCTCCTCTGCAAAAAAACTGCCCAGATCCTGAGCTCTGCCGCCAGAGCTTCAGACGCTT
TTGTTATCAAGAGGTGTCTGGACCCCAAGAGGCGCTCTCCCAGCTCCGACAGCTCTGCCG
TCAGTGGTTGCAACCCGAGCTGCACACCAAGGAGCAGATTTTGGAGCTTCTGGTGATGGA
GCAGTTCCTGACCATCCTGCCCCCGGAGATCCAGGCTCGGGTCAGGCATCGATGTCCAAT
GAGCAGCAAGGAGATTGTGACCCTCGTGGAAGATTTTCACAGAGCATCCAAGAAACCAAA
GCAGTGGGTGGCCGTTTGTATGCAGGGGCAAAAGGTGCTCTTGGAGAAAACTGGATCTCA
GCTTGGAGAACAGGAACTGCCAGACTTTCAACCGCAGACTCCTAGGAGAGATCTCAGGGA
GAGCTCTCCAGCAGAGCCTTCCCAGGCAGGAGCTTATGACCGGCTGAGCCCCCATCATTG
GGAGAAATCCCCACTCCTCCAAGAACCAACCCCCAAATTGGCTGGGACAGAGGCCCCCAG
AATGAGAAGTGACAACAAGGAAAATCCACAACAGGAAGGGGCTAAAGGAGCAAAGCCATG
TGCAGTGTCAGCTGGCAGATCCAAAGGGAATGGTCTGCAGAATCCTGAACCAAGAGGGGC
AAATATGAGTGAACCTCGGTTGTCACGGAGGCAGGTCAGCTCCCCAAATGCTCAAAAGCC
ATTTGCTCACTACCAGAGACATTGCAGGGTGGAATACATCAGCAGCCCCCTAAAAAGCCA
CCCACTGAGAGAGCTAAAGAAAAGCAAAGGAGGTAAACGGAGTCTGAGCAACCGTTTGCA
ACATCTTGGTCACCAGCCCACCCGCTCAGCAAAGAAACCCTACAAATGTGATGACTGTGG
GAAAAGCTTCACGTGGAATTCAGAGCTGAAGAGACACAAGAGAGTCCACACAGGAGAGAG
ACCCTACACGTGCGGAGAGTGTGGAAACTGCTTTGGGCGGCAGTCAACCCTGAAGCTGCA
CCAGAGGATCCACACTGGAGAGAAGCCATACCAGTGTGGCCAGTGTGGGAAAAGCTTTCG
CCAGAGCTCAAACCTTCACCAGCATCACCGACTTCACCATGGGGACGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT