Gene/Protein Characteristic Table for FHC03359

Species
human

Product ID
FHC03359

Corresponding
Flexi Clone PID

ORF Sequence
DNA sequence (1131 bp)
Protein sequence (377 aa)

Linker Type
Flexi type
The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site ...

Cloning Site
SgfI-PmeI

GenBank Accession
ORF sequence is derived from [AB464678]

Gene
KIBB9849

Symbol
P2RY2
Alias : HP2U, P2RU1, P2U, P2U1, P2UR, P2Y2, P2Y2R

Description
purinergic receptor P2Y, G-protein coupled, 2

Original Clone ID
cp00418

Quality control data of this clone

Characterization of the cloned ORF

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Features of the cloned ORF sequence

Length: 1131 bp
Restriction map A Restriction map B
Features of the protein sequence

Length: 377 aa
Result of homology search against nr database ( FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAW74882 2.5e-169 100.0 purinergic rece...
Homo sapiens
NP_002555 4e-168 99.7 P2Y purinocepto...
Homo sapiens
XP_004051814 8.9e-168 99.5 P2Y purinocepto...
Gorilla gorilla...
AAH12104 1.4e-167 99.5 Purinergic rece...
Homo sapiens
P41231 1.7e-167 99.5 P2Y purinocepto...
Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
[ zoom out (x2) ] PR00594 PR00237 PR00594 PR00237 PR01157 PR01157 PR00237 PR01157 PR00594 PR00237 PR00594 PR00237 PR01157 PR00237 PR01157 PR00594 PR00237 PR00594 PR00594 PR00594 PF00001 PS50262 PS00237

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR000356 8 26 PR00594 P2U purinoceptor
IPR000276 35 59 PR00237 Rhodopsin-like GPCR superfamily
IPR000356 59 70 PR00594 P2U purinoceptor
IPR000276 68 89 PR00237 Rhodopsin-like GPCR superfamily
IPR002286 82 93 PR01157 P2 purinoceptor
IPR002286 106 123 PR01157 P2 purinoceptor
IPR000276 113 135 PR00237 Rhodopsin-like GPCR superfamily
IPR002286 130 140 PR01157 P2 purinoceptor
IPR000356 143 152 PR00594 P2U purinoceptor
IPR000276 149 170 PR00237 Rhodopsin-like GPCR superfamily
IPR000356 164 180 PR00594 P2U purinoceptor
IPR000276 199 222 PR00237 Rhodopsin-like GPCR superfamily
IPR002286 217 228 PR01157 P2 purinoceptor
IPR000276 243 267 PR00237 Rhodopsin-like GPCR superfamily
IPR002286 257 268 PR01157 P2 purinoceptor
IPR000356 270 280 PR00594 P2U purinoceptor
IPR000276 288 314 PR00237 Rhodopsin-like GPCR superfamily
IPR000356 311 324 PR00594 P2U purinoceptor
IPR000356 331 344 PR00594 P2U purinoceptor
IPR000356 356 368 PR00594 P2U purinoceptor
HMMPfam IPR000276 50 306 PF00001 Rhodopsin-like GPCR superfamily
ProfileScan IPR000276 50 306 PS50262 Rhodopsin-like GPCR superfamily
ScanRegExp IPR000276 119 135 PS00237 Rhodopsin-like GPCR superfamily

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 36 LPVSYGVVCVLGLCLNAVALYIF 58 PRIMARY 23
2 71 YMFHLAVSDALYAASLPLLVYYY 93 SECONDARY 23
3 106 CKLVRFLFYTNLYCSILFLTCIS 128 SECONDARY 23
4 153 VAGAVWVLVLACQAPVLYFVTTS 175 PRIMARY 23
5 200 SVMLGLLFAVPFAVILVCYVLMA 222 PRIMARY 23
6 243 SVRTIAVVLAVFALCFLPFHVTR 265 PRIMARY 23

Result of homology search against disease genes in OMIM database
(FASTA output, Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%)
NP_788086 377 600041 99.4 100.0
NP_002555 377 600041 99.4 100.0
NP_788085 377 600041 99.4 100.0
NP_002556 365 300038 79.8 83.0
NP_002554 373 601167 67.6 83.6
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