Gene/Protein Characteristic Table for FHC03038

Species
human

Product ID
FHC03038

Corresponding
Flexi Clone PID

ORF Sequence
DNA sequence (1083 bp)
Protein sequence (361 aa)

Linker Type
Flexi type
The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site ...

Cloning Site
SgfI-PmeI

GenBank Accession
ORF sequence is derived from [AB464515]

Gene
KIBB8810

Symbol
GPR183
Alias : EBI2

Description
G protein-coupled receptor 183

Original Clone ID
cp00845

Quality control data of this clone

Characterization of the cloned ORF

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Features of the cloned ORF sequence

Length: 1083 bp
Restriction map A Restriction map B
Features of the protein sequence

Length: 361 aa
Result of homology search against nr database ( FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
NP_004942 1e-160 100.0 G-protein coupl...
Homo sapiens
BAD97110 2.2e-160 99.7 EBV-induced G p...
Homo sapiens
XP_001146657 6e-160 99.7 G-protein coupl...
Pan troglodytes
BAG36232 9.7e-160 99.4 unnamed protein...
Homo sapiens
XP_003279264 1.1e-159 99.4 LOW QUALITY PRO...
Nomascus leucogenys

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
[ zoom out (x2) ] PR00237 PR00237 PR01157 PR01157 PR00237 PR01157 PR00237 PR00237 PR01157 PR00237 PR01157 PR00237 PF00001 PS50262 PS00237

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR000276 33 57 PR00237 Rhodopsin-like GPCR superfamily
IPR000276 66 87 PR00237 Rhodopsin-like GPCR superfamily
IPR002286 80 91 PR01157 P2 purinoceptor
IPR002286 104 121 PR01157 P2 purinoceptor
IPR000276 111 133 PR00237 Rhodopsin-like GPCR superfamily
IPR002286 128 138 PR01157 P2 purinoceptor
IPR000276 147 168 PR00237 Rhodopsin-like GPCR superfamily
IPR000276 197 220 PR00237 Rhodopsin-like GPCR superfamily
IPR002286 215 226 PR01157 P2 purinoceptor
IPR000276 242 266 PR00237 Rhodopsin-like GPCR superfamily
IPR002286 256 267 PR01157 P2 purinoceptor
IPR000276 290 316 PR00237 Rhodopsin-like GPCR superfamily
HMMPfam IPR000276 48 308 PF00001 Rhodopsin-like GPCR superfamily
ProfileScan IPR000276 48 308 PS50262 Rhodopsin-like GPCR superfamily
ScanRegExp IPR000276 117 133 PS00237 Rhodopsin-like GPCR superfamily

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 36 LHYSLVFIIGLVGNLLALVVIVQ 58 PRIMARY 23
2 97 WRIGDALCRITALVFYINTYAGV 119 SECONDARY 23
3 149 KGVCIFVWILVFAQTLPLLINPM 171 SECONDARY 23
4 194 PWILLGACFIGYVLPLIIILICY 216 PRIMARY 23
5 245 TIILIIVVFVLCFTPYHVAIIQH 267 PRIMARY 23

Result of homology search against disease genes in OMIM database
(FASTA output, Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%)
NP_004942 361 605741 100.0 100.0
NP_006630 337 300201 67.5 85.6
NP_005758 344 609239 66.3 80.9
NP_005287 370 300086 64.8 88.1
NP_002554 373 601167 63.6 85.3
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