GPR183 ORF clone FHC03038

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Halotag ORF Clone FHC03038

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Clone info.

Species

human

Clone Name

pFN21AB8810 Sequence (with vector) Restriction Map

Product ID

FHC03038

Flexi Clone PID

FXC03038

ORF Sequence

DNA sequence (1083 bp)
Protein sequence (361 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464515]

Gene

KIBB8810

Symbol

GPR183

Alias : EBI2, hEBI2

Description

G protein-coupled receptor 183

Original Clone ID

cp00845

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1083 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 361 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9492.1 2.4e-129 100.0 GPR183 CCDS14439.1 7.7e-28 29.3 CYSLTR1 CCDS9410.1 7.8e-28 31.3 LPAR6 CCDS14441.1 7.3e-27 30.6 LPAR4 CCDS3169.1 1.7e-24 29.0 P2RY1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 33 57 PR00237 GPCR IPR000276 66 87 PR00237 GPCR IPR002286 80 91 PR01157 P2 purinoceptor IPR002286 104 121 PR01157 P2 purinoceptor IPR000276 111 133 PR00237 GPCR IPR002286 128 138 PR01157 P2 purinoceptor IPR000276 147 168 PR00237 GPCR IPR000276 197 220 PR00237 GPCR IPR002286 215 226 PR01157 P2 purinoceptor IPR000276 242 266 PR00237 GPCR IPR002286 256 267 PR01157 P2 purinoceptor IPR000276 290 316 PR00237 GPCR HMMPfam IPR000276 48 308 PF00001 GPCR ProfileScan IPR017452 48 308 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 36 LHYSLVFIIGLVGNLLALVVIVQ 58 PRIMARY 23 2 97 WRIGDALCRITALVFYINTYAGV 119 SECONDARY 23 3 149 KGVCIFVWILVFAQTLPLLINPM 171 SECONDARY 23 4 194 PWILLGACFIGYVLPLIIILICY 216 PRIMARY 23 5 245 TIILIIVVFVLCFTPYHVAIIQH 267 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016875894 361 605741 100.0 100.0 NP_004942 361 605741 100.0 100.0 NP_001155970 344 278150,609239 31.3 80.9 NP_001155969 344 278150,609239 31.3 80.9 NP_005758 344 278150,609239 31.3 80.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8810 Download

>KIBB8810  1083 bp
ATGGATATACAAATGGCAAACAATTTTACTCCGCCCTCTGCAACTCCTCAGGGAAATGAC
TGTGACCTCTATGCACATCACAGCACGGCCAGGATAGTAATGCCTCTGCATTACAGCCTC
GTCTTCATCATTGGGCTCGTGGGAAACTTACTAGCCTTGGTCGTCATTGTTCAAAACAGG
AAAAAAATCAACTCTACCACCCTCTATTCAACAAATTTGGTGATTTCTGATATACTTTTT
ACCACCGCTTTGCCTACACGAATAGCCTACTATGCAATGGGCTTTGACTGGAGAATCGGA
GATGCCTTGTGTAGGATAACTGCGCTAGTGTTTTACATCAACACATATGCAGGTGTGAAC
TTTATGACCTGCCTGAGTATTGACCGCTTCATTGCTGTGGTGCACCCTCTACGCTACAAC
AAGATAAAAAGGATTGAACATGCAAAAGGCGTGTGCATATTTGTCTGGATTCTAGTATTT
GCTCAGACACTCCCACTCCTCATCAACCCTATGTCAAAGCAGGAGGCTGAAAGGATTACA
TGCATGGAGTATCCAAACTTTGAAGAAACTAAATCTCTTCCCTGGATTCTGCTTGGGGCA
TGTTTCATAGGATATGTACTTCCACTTATAATCATTCTCATCTGCTATTCTCAGATCTGC
TGCAAACTCTTCAGAACTGCCAAACAAAACCCACTCACTGAGAAATCTGGTGTAAACAAA
AAGGCTCTCAACACAATTATTCTTATTATTGTTGTGTTTGTTCTCTGTTTCACACCTTAC
CATGTTGCAATTATTCAACATATGATTAAGAAGCTTCGTTTCTCTAATTTCCTGGAATGT
AGCCAAAGACATTCGTTCCAGATTTCTCTGCACTTTACAGTATGCCTGATGAACTTCAAT
TGCTGCATGGACCCTTTTATCTACTTCTTTGCATGTAAAGGGTATAAGAGAAAGGTTATG
AGGATGCTGAAACGGCAAGTCAGTGTATCGATTTCTAGTGCTGTGAAGTCAGCCCCTGAA
GAAAATTCACGTGAAATGACAGAAACGCAGATGATGATACATTCCAAGTCTTCAAATGGA
AAG

ORF Protein

Cloned ORF protein sequence for pFN21AB8810 Download

>KIBB8810  361 aa
MDIQMANNFTPPSATPQGNDCDLYAHHSTARIVMPLHYSLVFIIGLVGNLLALVVIVQNR
KKINSTTLYSTNLVISDILFTTALPTRIAYYAMGFDWRIGDALCRITALVFYINTYAGVN
FMTCLSIDRFIAVVHPLRYNKIKRIEHAKGVCIFVWILVFAQTLPLLINPMSKQEAERIT
CMEYPNFEETKSLPWILLGACFIGYVLPLIIILICYSQICCKLFRTAKQNPLTEKSGVNK
KALNTIILIIVVFVLCFTPYHVAIIQHMIKKLRFSNFLECSQRHSFQISLHFTVCLMNFN
CCMDPFIYFFACKGYKRKVMRMLKRQVSVSISSAVKSAPEENSREMTETQMMIHSKSSNG
K

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8810 Download

>pFN21AB8810  5788 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATATACAAATGGCAAACAATTTTACTCCGCC
CTCTGCAACTCCTCAGGGAAATGACTGTGACCTCTATGCACATCACAGCACGGCCAGGAT
AGTAATGCCTCTGCATTACAGCCTCGTCTTCATCATTGGGCTCGTGGGAAACTTACTAGC
CTTGGTCGTCATTGTTCAAAACAGGAAAAAAATCAACTCTACCACCCTCTATTCAACAAA
TTTGGTGATTTCTGATATACTTTTTACCACCGCTTTGCCTACACGAATAGCCTACTATGC
AATGGGCTTTGACTGGAGAATCGGAGATGCCTTGTGTAGGATAACTGCGCTAGTGTTTTA
CATCAACACATATGCAGGTGTGAACTTTATGACCTGCCTGAGTATTGACCGCTTCATTGC
TGTGGTGCACCCTCTACGCTACAACAAGATAAAAAGGATTGAACATGCAAAAGGCGTGTG
CATATTTGTCTGGATTCTAGTATTTGCTCAGACACTCCCACTCCTCATCAACCCTATGTC
AAAGCAGGAGGCTGAAAGGATTACATGCATGGAGTATCCAAACTTTGAAGAAACTAAATC
TCTTCCCTGGATTCTGCTTGGGGCATGTTTCATAGGATATGTACTTCCACTTATAATCAT
TCTCATCTGCTATTCTCAGATCTGCTGCAAACTCTTCAGAACTGCCAAACAAAACCCACT
CACTGAGAAATCTGGTGTAAACAAAAAGGCTCTCAACACAATTATTCTTATTATTGTTGT
GTTTGTTCTCTGTTTCACACCTTACCATGTTGCAATTATTCAACATATGATTAAGAAGCT
TCGTTTCTCTAATTTCCTGGAATGTAGCCAAAGACATTCGTTCCAGATTTCTCTGCACTT
TACAGTATGCCTGATGAACTTCAATTGCTGCATGGACCCTTTTATCTACTTCTTTGCATG
TAAAGGGTATAAGAGAAAGGTTATGAGGATGCTGAAACGGCAAGTCAGTGTATCGATTTC
TAGTGCTGTGAAGTCAGCCCCTGAAGAAAATTCACGTGAAATGACAGAAACGCAGATGAT
GATACATTCCAAGTCTTCAAATGGAAAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT