ARHGEF3 ORF clone FHC02839

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02839

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Clone info.

Species

human

Clone Name

pFN21AB8437 Sequence (with vector) Restriction Map

Product ID

FHC02839

Flexi Clone PID

FXC02839

ORF Sequence

DNA sequence (1578 bp)
Protein sequence (526 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463227]

Gene

KIBB8437

Symbol

ARHGEF3

Alias : GEF3, STA3, XPLN

Description

Rho guanine nucleotide exchange factor (GEF) 3, transcript variant 3

Original Clone ID

oc00190

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1578 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 526 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2878.1 1.3e-201 100.0 ARHGEF3 CCDS74948.1 2.9e-189 96.9 ARHGEF3 CCDS46854.1 3e-189 96.9 ARHGEF3 CCDS46855.1 4.4e-189 97.3 ARHGEF3 CCDS7067.1 2e-105 62.1 NET1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000219 127 302 PF00621 Dbl homology (DH) domain HMMSmart IPR000219 126 303 SM00325 Dbl homology (DH) domain IPR001849 312 451 SM00233 Pleckstrin homology domain ProfileScan IPR000219 122 304 PS50010 Dbl homology (DH) domain IPR001849 291 449 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_062455 526 612115 100.0 100.0 XP_011532068 527 612115 96.9 97.7 XP_011532067 527 612115 96.9 97.7 NP_001276627 532 612115 96.9 97.7 XP_016861991 538 612115 96.9 97.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8437 Download

>KIBB8437  1578 bp
ATGGTGGCCAAGGATTACCCCTTCTACCTCACGGTCAAGAGAGCGAACTGCAGCCTGGAG
CTACCCCCGGCCAGCGGTCCGGCCAAGGACGCTGAGGAGCCTAGTAATAAACGGGTCAAA
CCCCTTTCCCGAGTCACGTCGCTAGCAAACCTCATCCCGCCCGTGAAGGCCACGCCATTA
AAGCGCTTCAGTCAAACCCTGCAGCGCTCCATTAGCTTCCGCAGTGAGAGCCGCCCTGAC
ATCCTCGCCCCCCGACCCTGGTCCAGAAATGCCGCCCCCTCGAGCACGAAACGGAGAGAT
AGCAAGCTGTGGAGTGAGACCTTCGATGTGTGCGTCAATCAGATGCTTACATCCAAGGAA
ATCAAACGTCAGGAGGCGATCTTTGAGCTTTCCCAAGGAGAAGAAGACTTGATAGAAGAC
TTGAAATTAGCAAAAAAGGCCTATCATGACCCCATGCTGAAACTCTCCATAATGACAGAA
CAAGAGTTGAATCAAATTTTTGGAACACTGGACTCTCTAATTCCTCTACATGAAGAGCTC
CTTAGTCAGCTTCGAGATGTTAGGAAGCCTGATGGCTCGACTGAACATGTTGGTCCCATC
CTCGTGGGCTGGCTCCCTTGCCTCAGCTCCTATGATAGCTACTGCAGCAATCAAGTAGCC
GCCAAAGCTCTGCTGGACCACAAAAAGCAAGATCACCGAGTCCAGGATTTCCTACAGCGA
TGTTTAGAATCCCCCTTTAGCCGCAAACTAGATCTCTGGAATTTCCTCGATATTCCAAGA
AGCCGCCTGGTAAAATACCCTCTGCTTCTCCGAGAAATCTTGAGGCACACACCAAATGAT
AATCCAGATCAGCAGCACTTGGAAGAAGCTATAAATATCATTCAGGGAATTGTGGCAGAA
ATCAACACCAAGACTGGTGAATCTGAATGCCGCTATTATAAAGAGCGGCTTCTTTACTTG
GAAGAAGGCCAGAAAGACTCCCTGATCGACAGCTCTCGAGTCTTGTGTTGTCATGGTGAA
CTGAAGAACAATCGGGGCGTGAAACTGCATGTTTTCCTGTTCCAAGAAGTGCTTGTGATC
ACTCGAGCCGTCACCCACAATGAGCAGCTTTGCTACCAGCTGTACCGTCAGCCAATCCCC
GTGAAAGACCTCCTGCTGGAAGACCTCCAGGATGGAGAAGTGAGGCTGGGTGGCTCCCTG
CGAGGGGCATTCAGCAACAATGAGAGAATTAAAAACTTCTTCAGAGTCAGTTTCAAAAAT
GGATCCCAAAGTCAGACCCACTCGCTACAAGCCAATGACACTTTCAACAAACAGCAGTGG
CTTAACTGTATTCGTCAAGCCAAAGAAACAGTTTTGTGTGCTGCCGGGCAAGCTGGGGTG
CTTGACTCCGAGGGATCGTTCCTAAATCCCACCACCGGGAGCAGAGAGCTACAGGGAGAA
ACAAAACTTGAGCAGATGGACCAATCGGACAGTGAGTCAGACTGTAGTATGGACACGAGT
GAGGTCAGCCTCGACTGTGAGCGCATGGAACAGACAGACTCTTCCTGTGGAAACAGCAGG
CACGGTGAAAGTAACGTC

ORF Protein

Cloned ORF protein sequence for pFN21AB8437 Download

>KIBB8437  526 aa
MVAKDYPFYLTVKRANCSLELPPASGPAKDAEEPSNKRVKPLSRVTSLANLIPPVKATPL
KRFSQTLQRSISFRSESRPDILAPRPWSRNAAPSSTKRRDSKLWSETFDVCVNQMLTSKE
IKRQEAIFELSQGEEDLIEDLKLAKKAYHDPMLKLSIMTEQELNQIFGTLDSLIPLHEEL
LSQLRDVRKPDGSTEHVGPILVGWLPCLSSYDSYCSNQVAAKALLDHKKQDHRVQDFLQR
CLESPFSRKLDLWNFLDIPRSRLVKYPLLLREILRHTPNDNPDQQHLEEAINIIQGIVAE
INTKTGESECRYYKERLLYLEEGQKDSLIDSSRVLCCHGELKNNRGVKLHVFLFQEVLVI
TRAVTHNEQLCYQLYRQPIPVKDLLLEDLQDGEVRLGGSLRGAFSNNERIKNFFRVSFKN
GSQSQTHSLQANDTFNKQQWLNCIRQAKETVLCAAGQAGVLDSEGSFLNPTTGSRELQGE
TKLEQMDQSDSESDCSMDTSEVSLDCERMEQTDSSCGNSRHGESNV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8437 Download

>pFN21AB8437  6283 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTGGCCAAGGATTACCCCTTCTACCTCACGGT
CAAGAGAGCGAACTGCAGCCTGGAGCTACCCCCGGCCAGCGGTCCGGCCAAGGACGCTGA
GGAGCCTAGTAATAAACGGGTCAAACCCCTTTCCCGAGTCACGTCGCTAGCAAACCTCAT
CCCGCCCGTGAAGGCCACGCCATTAAAGCGCTTCAGTCAAACCCTGCAGCGCTCCATTAG
CTTCCGCAGTGAGAGCCGCCCTGACATCCTCGCCCCCCGACCCTGGTCCAGAAATGCCGC
CCCCTCGAGCACGAAACGGAGAGATAGCAAGCTGTGGAGTGAGACCTTCGATGTGTGCGT
CAATCAGATGCTTACATCCAAGGAAATCAAACGTCAGGAGGCGATCTTTGAGCTTTCCCA
AGGAGAAGAAGACTTGATAGAAGACTTGAAATTAGCAAAAAAGGCCTATCATGACCCCAT
GCTGAAACTCTCCATAATGACAGAACAAGAGTTGAATCAAATTTTTGGAACACTGGACTC
TCTAATTCCTCTACATGAAGAGCTCCTTAGTCAGCTTCGAGATGTTAGGAAGCCTGATGG
CTCGACTGAACATGTTGGTCCCATCCTCGTGGGCTGGCTCCCTTGCCTCAGCTCCTATGA
TAGCTACTGCAGCAATCAAGTAGCCGCCAAAGCTCTGCTGGACCACAAAAAGCAAGATCA
CCGAGTCCAGGATTTCCTACAGCGATGTTTAGAATCCCCCTTTAGCCGCAAACTAGATCT
CTGGAATTTCCTCGATATTCCAAGAAGCCGCCTGGTAAAATACCCTCTGCTTCTCCGAGA
AATCTTGAGGCACACACCAAATGATAATCCAGATCAGCAGCACTTGGAAGAAGCTATAAA
TATCATTCAGGGAATTGTGGCAGAAATCAACACCAAGACTGGTGAATCTGAATGCCGCTA
TTATAAAGAGCGGCTTCTTTACTTGGAAGAAGGCCAGAAAGACTCCCTGATCGACAGCTC
TCGAGTCTTGTGTTGTCATGGTGAACTGAAGAACAATCGGGGCGTGAAACTGCATGTTTT
CCTGTTCCAAGAAGTGCTTGTGATCACTCGAGCCGTCACCCACAATGAGCAGCTTTGCTA
CCAGCTGTACCGTCAGCCAATCCCCGTGAAAGACCTCCTGCTGGAAGACCTCCAGGATGG
AGAAGTGAGGCTGGGTGGCTCCCTGCGAGGGGCATTCAGCAACAATGAGAGAATTAAAAA
CTTCTTCAGAGTCAGTTTCAAAAATGGATCCCAAAGTCAGACCCACTCGCTACAAGCCAA
TGACACTTTCAACAAACAGCAGTGGCTTAACTGTATTCGTCAAGCCAAAGAAACAGTTTT
GTGTGCTGCCGGGCAAGCTGGGGTGCTTGACTCCGAGGGATCGTTCCTAAATCCCACCAC
CGGGAGCAGAGAGCTACAGGGAGAAACAAAACTTGAGCAGATGGACCAATCGGACAGTGA
GTCAGACTGTAGTATGGACACGAGTGAGGTCAGCCTCGACTGTGAGCGCATGGAACAGAC
AGACTCTTCCTGTGGAAACAGCAGGCACGGTGAAAGTAACGTCGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT