DOK4 ORF clone FHC02609

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02609

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Clone info.

Species

human

Clone Name

pFN21AB8139 Sequence (with vector) Restriction Map

Product ID

FHC02609

Flexi Clone PID

FXC02609

ORF Sequence

DNA sequence (978 bp)
Protein sequence (326 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463576]

Gene

KIBB8139

Symbol

DOK4

Alias : IRS-5, IRS5

Description

docking protein 4

Original Clone ID

cp00499

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 978 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 326 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10783.1 9.4e-145 100.0 DOK4 CCDS81986.1 1.3e-128 89.1 DOK4 CCDS32841.1 8.9e-91 63.9 DOK6 CCDS13446.1 1.5e-78 64.7 DOK5 CCDS13447.1 1.4e-45 66.9 DOK5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 8 107 PF00169 Pleckstrin homology domain IPR002404 137 230 PF02174 Insulin receptor substrate-1 HMMSmart IPR001849 8 114 SM00233 Pleckstrin homology domain IPR002404 130 232 SM00310 Insulin receptor substrate-1 ProfileScan IPR002404 132 237 PS51064 Insulin receptor substrate-1
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060580 326 608333 100.0 100.0 XP_005256100 326 608333 100.0 100.0 NP_001317485 365 608333 89.1 97.9 NP_689934 331 611402 63.9 99.4 XP_011524177 289 611402 68.9 88.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8139 Download

>KIBB8139  978 bp
ATGGCGACCAATTTCAGTGACATCGTCAAGCAAGGCTACGTGAAGATGAAGAGCAGGAAG
CTCGGGATCTACCGGAGGTGCTGGCTGGTGTTCCGGAAATCCTCCAGCAAGGGGCCCCAG
CGGCTGGAGAAGTATCCAGATGAGAAGTCGGTGTGCCTCCGGGGCTGCCCCAAGGTGACT
GAGATCAGCAACGTCAAGTGTGTTACGCGGCTCCCCAAGGAGACCAAGCGGCAGGCGGTG
GCCATCATATTCACTGATGACTCGGCACGTACCTTCACCTGCGACTCAGAGCTAGAGGCA
GAGGAGTGGTACAAGACACTATCTGTGGAGTGTCTGGGGTCCCGCCTCAACGACATCAGT
CTGGGAGAACCTGACCTCCTGGCCCCAGGGGTGCAGTGTGAACAGACAGATCGCTTCAAT
GTCTTCCTGCTGCCCTGCCCCAACCTGGACGTGTATGGCGAGTGCAAGCTGCAGATCACC
CACGAGAACATCTACCTCTGGGACATCCACAACCCCCGTGTGAAGCTCGTCTCGTGGCCC
CTCTGCTCACTGCGCCGCTATGGCCGGGATGCCACACGCTTTACCTTCGAGGCTGGCCGG
ATGTGTGATGCTGGGGAAGGACTCTATACCTTCCAGACACAAGAGGGGGAGCAGATTTAC
CAGCGCGTCCACAGTGCCACCCTGGCCATCGCAGAGCAGCACAAGCGGGTCCTGCTGGAA
ATGGAGAAGAACGTGAGGCTGCTGAACAAGGGCACGGAACATTACTCGTATCCCTGCACA
CCCACGACCATGCTGCCGCGCAGTGCCTACTGGCACCACATCACTGGTTCCCAGAACATC
GCCGAAGCCTCCAGCTATGCTGGTGAGGGGTATGGGGCAGCCCAGGCCAGCTCGGAAACA
GACCTCCTCAACAGATTCATCCTGCTAAAGCCAAAGCCCAGCCAGGGGGACAGCAGTGAG
GCCAAGACCCCATCCCAG

ORF Protein

Cloned ORF protein sequence for pFN21AB8139 Download

>KIBB8139  326 aa
MATNFSDIVKQGYVKMKSRKLGIYRRCWLVFRKSSSKGPQRLEKYPDEKSVCLRGCPKVT
EISNVKCVTRLPKETKRQAVAIIFTDDSARTFTCDSELEAEEWYKTLSVECLGSRLNDIS
LGEPDLLAPGVQCEQTDRFNVFLLPCPNLDVYGECKLQITHENIYLWDIHNPRVKLVSWP
LCSLRRYGRDATRFTFEAGRMCDAGEGLYTFQTQEGEQIYQRVHSATLAIAEQHKRVLLE
MEKNVRLLNKGTEHYSYPCTPTTMLPRSAYWHHITGSQNIAEASSYAGEGYGAAQASSET
DLLNRFILLKPKPSQGDSSEAKTPSQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8139 Download

>pFN21AB8139  5683 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGACCAATTTCAGTGACATCGTCAAGCAAGG
CTACGTGAAGATGAAGAGCAGGAAGCTCGGGATCTACCGGAGGTGCTGGCTGGTGTTCCG
GAAATCCTCCAGCAAGGGGCCCCAGCGGCTGGAGAAGTATCCAGATGAGAAGTCGGTGTG
CCTCCGGGGCTGCCCCAAGGTGACTGAGATCAGCAACGTCAAGTGTGTTACGCGGCTCCC
CAAGGAGACCAAGCGGCAGGCGGTGGCCATCATATTCACTGATGACTCGGCACGTACCTT
CACCTGCGACTCAGAGCTAGAGGCAGAGGAGTGGTACAAGACACTATCTGTGGAGTGTCT
GGGGTCCCGCCTCAACGACATCAGTCTGGGAGAACCTGACCTCCTGGCCCCAGGGGTGCA
GTGTGAACAGACAGATCGCTTCAATGTCTTCCTGCTGCCCTGCCCCAACCTGGACGTGTA
TGGCGAGTGCAAGCTGCAGATCACCCACGAGAACATCTACCTCTGGGACATCCACAACCC
CCGTGTGAAGCTCGTCTCGTGGCCCCTCTGCTCACTGCGCCGCTATGGCCGGGATGCCAC
ACGCTTTACCTTCGAGGCTGGCCGGATGTGTGATGCTGGGGAAGGACTCTATACCTTCCA
GACACAAGAGGGGGAGCAGATTTACCAGCGCGTCCACAGTGCCACCCTGGCCATCGCAGA
GCAGCACAAGCGGGTCCTGCTGGAAATGGAGAAGAACGTGAGGCTGCTGAACAAGGGCAC
GGAACATTACTCGTATCCCTGCACACCCACGACCATGCTGCCGCGCAGTGCCTACTGGCA
CCACATCACTGGTTCCCAGAACATCGCCGAAGCCTCCAGCTATGCTGGTGAGGGGTATGG
GGCAGCCCAGGCCAGCTCGGAAACAGACCTCCTCAACAGATTCATCCTGCTAAAGCCAAA
GCCCAGCCAGGGGGACAGCAGTGAGGCCAAGACCCCATCCCAGGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT