ASH2L ORF clone FHC01483

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01483

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Clone info.

Species

human

Clone Name

pFN21AB5154 Sequence (with vector) Restriction Map

Product ID

FHC01483

Flexi Clone PID

FXC01483

Original Clone PID

ORK01483

ORF Sequence

DNA sequence (1884 bp)
Protein sequence (628 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385056]

Gene

KIBB5154

Symbol

ASH2L

Alias : ASH2, ASH2L1, ASH2L2, Bre2

Description

ash2 (absent, small, or homeotic)-like (Drosophila), transcript variant 1

Original Clone ID

bm00505

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1884 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 628 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6101.1 9.1e-196 100.0 ASH2L CCDS47840.1 1.2e-167 100.0 ASH2L CCDS64872.1 5.8e-153 100.0 ASH2L CCDS59100.1 7.9e-139 93.8 ASH2L The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003877 418 495 PF00622 SPla/RYanodine receptor SPRY HMMSmart IPR018355 418 582 SM00449 SPla/RYanodine receptor subgroup ProfileScan IPR001870 360 583 PS50188 B302/SPRY domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004665 628 604782 100.0 100.0 XP_005273739 634 604782 99.1 100.0 NP_001098684 534 604782 100.0 85.0 XP_006716476 595 604782 94.7 100.0 XP_005273740 540 604782 98.9 85.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5154 Download

>KIBB5154  1884 bp
ATGGCGGCGGCAGGAGCAGGACCTGGCCAGGAAGCGGGTGCCGGGCCTGGCCCAGGAGCG
GTCGCAAATGCAACAGGGGCAGAAGAGGGGGAGATGAAGCCGGTGGCAGCGGGAGCAGCC
GCTCCTCCTGGAGAGGGGATCTCTGCTGCTCCGACAGTTGAGCCCAGTTCCGGGGAGGCT
GAAGGCGGGGAGGCAAACTTGGTCGATGTAAGCGGTGGCTTGGAGACAGAATCATCTAAT
GGAAAAGATACACTAGAAGGTGCTGGGGATACATCAGAGGTGATGGATACTCAGGCGGGC
TCCGTGGATGAAGAGAATGGCCGACAGTTGGGTGAGGTAGAGCTGCAATGTGGGATTTGT
ACAAAATGGTTCACGGCTGACACATTTGGCATAGATACCTCATCCTGTCTACCTTTCATG
ACCAACTACAGTTTTCATTGCAACGTCTGCCATCACAGTGGGAATACCTATTTCCTCCGG
AAGCAAGCAAACTTGAAGGAAATGTGCCTTAGTGCTTTGGCCAACCTGACATGGCAGTCC
CGAACACAGGATGAACATCCGAAGACAATGTTCTCCAAAGATAAGGATATTATACCATTT
ATTGATAAATACTGGGAGTGCATGACAACCAGACAGAGACCTGGGAAAATGACTTGGCCA
AATAACATTGTTAAAACAATGAGTAAAGAAAGAGATGTATTCTTGGTAAAGGAACACCCA
GATCCAGGCAGTAAAGATCCAGAAGAAGATTACCCCAAATTTGGACTTTTGGATCAGGAC
CTTAGTAACATTGGTCCTGCTTATGACAACCAAAAACAGAGCAGTGCTGTGTCTACTAGT
GGGAATTTAAATGGGGGAATTGCAGCAGGAAGCAGCGGAAAAGGACGAGGAGCCAAGCGC
AAACAGCAGGATGGAGGGACCACAGGGACCACCAAGAAGGCCCGGAGTGACCCTTTGTTT
TCTGCTCAGCGCCTTCCCCCTCATGGCTACCCATTGGAACACCCGTTTAACAAAGATGGC
TATCGGTATATTCTAGCTGAGCCTGATCCGCACGCCCCTGACCCCGAGAAGCTGGAACTT
GACTGCTGGGCAGGAAAACCTATTCCTGGAGACCTCTACAGAGCCTGCTTGTATGAACGG
GTTTTGTTAGCCCTACATGATCGAGCTCCCCAGTTAAAGATCTCAGATGACCGGCTGACT
GTGGTTGGAGAGAAGGGCTACTCTATGGTGAGGGCCTCTCATGGAGTACGGAAGGGTGCC
TGGTATTTTGAAATCACTGTGGATGAGATGCCACCAGATACCGCTGCCAGACTGGGTTGG
TCCCAGCCCCTAGGAAACCTTCAAGCTCCTTTAGGTTATGATAAATTTAGCTATTCTTGG
CGGAGCAAAAAGGGAACCAAGTTCCACCAGTCCATTGGCAAACACTACTCTTCTGGCTAT
GGACAGGGAGACGTCCTGGGATTTTATATTAATCTTCCTGAAGACACAGAGACAGCCAAG
TCATTGCCAGACACATACAAAGATAAGGCTTTGATAAAATTCAAGAGTTATTTGTATTTT
GAGGAAAAAGACTTTGTGGATAAAGCAGAGAAGAGCCTGAAGCAGACTCCCCATAGTGAG
ATAATATTTTATAAAAATGGTGTCAATCAAGGTGTGGCTTACAAAGATATTTTTGAGGGG
GTTTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACGGTTTCCATTAACTTTGGACCA
TGCTTCAAGTATCCTCCGAAGGATCTCACTTACCGCCCTATGAGTGACATGGGCTGGGGC
GCCGTGGTAGAGCACACCCTGGCTGACGTCTTGTATCACGTGGAGACAGAAGTGGATGGG
AGGCGCAGTCCCCCATGGGAACCC

ORF Protein

Cloned ORF protein sequence for pFN21AB5154 Download

>KIBB5154  628 aa
MAAAGAGPGQEAGAGPGPGAVANATGAEEGEMKPVAAGAAAPPGEGISAAPTVEPSSGEA
EGGEANLVDVSGGLETESSNGKDTLEGAGDTSEVMDTQAGSVDEENGRQLGEVELQCGIC
TKWFTADTFGIDTSSCLPFMTNYSFHCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQS
RTQDEHPKTMFSKDKDIIPFIDKYWECMTTRQRPGKMTWPNNIVKTMSKERDVFLVKEHP
DPGSKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSTSGNLNGGIAAGSSGKGRGAKR
KQQDGGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLEL
DCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGA
WYFEITVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGY
GQGDVLGFYINLPEDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSE
IIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWG
AVVEHTLADVLYHVETEVDGRRSPPWEP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5154 Download

>pFN21AB5154  6589 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCGGCAGGAGCAGGACCTGGCCAGGAAGC
GGGTGCCGGGCCTGGCCCAGGAGCGGTCGCAAATGCAACAGGGGCAGAAGAGGGGGAGAT
GAAGCCGGTGGCAGCGGGAGCAGCCGCTCCTCCTGGAGAGGGGATCTCTGCTGCTCCGAC
AGTTGAGCCCAGTTCCGGGGAGGCTGAAGGCGGGGAGGCAAACTTGGTCGATGTAAGCGG
TGGCTTGGAGACAGAATCATCTAATGGAAAAGATACACTAGAAGGTGCTGGGGATACATC
AGAGGTGATGGATACTCAGGCGGGCTCCGTGGATGAAGAGAATGGCCGACAGTTGGGTGA
GGTAGAGCTGCAATGTGGGATTTGTACAAAATGGTTCACGGCTGACACATTTGGCATAGA
TACCTCATCCTGTCTACCTTTCATGACCAACTACAGTTTTCATTGCAACGTCTGCCATCA
CAGTGGGAATACCTATTTCCTCCGGAAGCAAGCAAACTTGAAGGAAATGTGCCTTAGTGC
TTTGGCCAACCTGACATGGCAGTCCCGAACACAGGATGAACATCCGAAGACAATGTTCTC
CAAAGATAAGGATATTATACCATTTATTGATAAATACTGGGAGTGCATGACAACCAGACA
GAGACCTGGGAAAATGACTTGGCCAAATAACATTGTTAAAACAATGAGTAAAGAAAGAGA
TGTATTCTTGGTAAAGGAACACCCAGATCCAGGCAGTAAAGATCCAGAAGAAGATTACCC
CAAATTTGGACTTTTGGATCAGGACCTTAGTAACATTGGTCCTGCTTATGACAACCAAAA
ACAGAGCAGTGCTGTGTCTACTAGTGGGAATTTAAATGGGGGAATTGCAGCAGGAAGCAG
CGGAAAAGGACGAGGAGCCAAGCGCAAACAGCAGGATGGAGGGACCACAGGGACCACCAA
GAAGGCCCGGAGTGACCCTTTGTTTTCTGCTCAGCGCCTTCCCCCTCATGGCTACCCATT
GGAACACCCGTTTAACAAAGATGGCTATCGGTATATTCTAGCTGAGCCTGATCCGCACGC
CCCTGACCCCGAGAAGCTGGAACTTGACTGCTGGGCAGGAAAACCTATTCCTGGAGACCT
CTACAGAGCCTGCTTGTATGAACGGGTTTTGTTAGCCCTACATGATCGAGCTCCCCAGTT
AAAGATCTCAGATGACCGGCTGACTGTGGTTGGAGAGAAGGGCTACTCTATGGTGAGGGC
CTCTCATGGAGTACGGAAGGGTGCCTGGTATTTTGAAATCACTGTGGATGAGATGCCACC
AGATACCGCTGCCAGACTGGGTTGGTCCCAGCCCCTAGGAAACCTTCAAGCTCCTTTAGG
TTATGATAAATTTAGCTATTCTTGGCGGAGCAAAAAGGGAACCAAGTTCCACCAGTCCAT
TGGCAAACACTACTCTTCTGGCTATGGACAGGGAGACGTCCTGGGATTTTATATTAATCT
TCCTGAAGACACAGAGACAGCCAAGTCATTGCCAGACACATACAAAGATAAGGCTTTGAT
AAAATTCAAGAGTTATTTGTATTTTGAGGAAAAAGACTTTGTGGATAAAGCAGAGAAGAG
CCTGAAGCAGACTCCCCATAGTGAGATAATATTTTATAAAAATGGTGTCAATCAAGGTGT
GGCTTACAAAGATATTTTTGAGGGGGTTTACTTCCCAGCCATCTCACTGTACAAGAGCTG
CACGGTTTCCATTAACTTTGGACCATGCTTCAAGTATCCTCCGAAGGATCTCACTTACCG
CCCTATGAGTGACATGGGCTGGGGCGCCGTGGTAGAGCACACCCTGGCTGACGTCTTGTA
TCACGTGGAGACAGAAGTGGATGGGAGGCGCAGTCCCCCATGGGAACCCGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT