EBF4 ORF clone FHC00838

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00838

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Clone info.

Species

human

Clone Name

pFN21ASDA1442 Sequence (with vector) Restriction Map

Product ID

FHC00838

Flexi Clone PID

FXC00838

ORF Sequence

DNA sequence (1620 bp)
Protein sequence (540 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384212]

Gene

KIAA1442

Symbol

EBF4

Alias : COE4, O/E-4, OE-4

Description

early B-cell factor 4

Original Clone ID

hh10127b

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1620 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 540 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46573.1 2.1e-113 95.2 EBF4 CCDS31314.1 1.4e-90 77.2 EBF3 CCDS4343.1 1.8e-88 75.1 EBF1 CCDS43726.1 4e-85 71.9 EBF2 CCDS78081.1 3.5e-53 71.9 EBF1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002909 252 323 PF01833 Cell surface receptor IPT/TIG HMMSmart IPR002909 251 335 SM00429 Cell surface receptor IPT/TIG
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001103984 598 609935 95.2 99.8 XP_006723663 571 609935 94.9 94.8 XP_016883472 619 609935 91.4 96.1 XP_016883473 646 609935 86.9 96.9 NP_001005463 551 607407 77.2 96.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1442 Download

>KIAA1442  1620 bp
ATGGACGCTCTGCCCCGCAGCGGGCTGAACCTGAAGGAGGAGCCGCTGCTGCCCGCCGGC
CTGGGCTCAGTGCGCTCCTGGATGCAGGGCGCGGGCATCCTGGACGCCAGCACCGCGGCG
CAGAGTGGCGTGGGTCTGGCACGAGCACATTTTGAGAAGCAGCCTCCCTCCAACCTCAGG
AAATCCAACTTCTTCCACTTCGTGCTGGCCATGTACGACCGGCAGGGGCAGCCCGTGGAG
GTGGAGCGCACAGCCTTCATCGACTTCGTGGAAAAGGACCGAGAGCCCGGGGCGGAAAAG
ACTAACAATGGGATCCATTACCGCCTCCGGCTGGTGTATAACAATGGACTGCGGACAGAG
CAAGACCTCTACGTGCGTCTCATCGACTCCATGTCCAAACAGGCCATCATCTATGAGGGG
CAGGACAAGAACCCCGAAATGTGCCGAGTGCTGCTCACCCATGAGATCATGTGCAGCCGG
TGCTGTGACCGGAAGAGCTGTGGCAACCGGAATGAGACGCCCTCAGACCCCGTCATCATT
GACAGGTTCTTCCTCAAGTTCTTCCTCAAATGCAACCAGAACTGCCTGAAGAATGCGGGG
AATCCCAGAGACATGCGCCGCTTCCAGGTGGTGGTGTCCACGACGGTGAGCGTGGACGGA
CACGTGCTGGCCGTGTCCGACAACATGTTTGTGCACAACAACTCCAAGCATGGCCGCAGG
GCGCGCCGCCTGGACCCCTCCGAAGCTGCCACCCCCTGCATCAAGGCCATCAGCCCCGGG
GAGGGCTGGACCACGGGCGGCGCCACCGTCATTGTCATCGGCGACAACTTCTTCGACGGG
TTGCAGGTCGTGTTCGGAAACGTGCTCGTGTGGAGCGAGCTCATCACGCCCCACGCCATC
CGGGTGCAGACGCCCCCGCGGCACATCCCCGGGGTGGTGGAGGTGACCCTCTCCTACAAG
TCCAAGCAGTTTTGCAAGGGATGCCCCGGCCGCTTTGTCTACACAGCTCTGAACGAGCCC
ACCATTGACTACGGATTCCAGAGGCTACAGAAAGTCATTCCCAGACACCCCGGAGACCCC
GAGAGGCTGCCCAAGGAAGTGCTGCTGAAGCGGGCGGCCGACTTGGCAGAAGCCCTGTAC
GGAGTGCCCGGCAGTAACCAGGAGCTGCTCCTGAAGCGCGCGGCGGACGTGGCCGAGGCT
CTGTACAGCACCCCCCGCGCACCCGGGCCGCTCGCACCCCTGGCCCCGAGCCACCCACAC
TCCGCCGTCGTGGGCATCAACGCCTTCAGCAGCCCGCTGGCCATCGCCGTCGGGGACGCC
ACCCCGGGGCCCGAGCCGGGCTACGCGCGCAGCTGCAGCAGCGCGTCCCCCCGCGGGTTC
GCGCCCAGCCCCGGCTCGCAGCAGAGCGGCTACGGCGGCGGCCTCGGAGCTGGCCTGGGC
GGCTACGGCGCGCCGGGCGTGGCCGGCCTCGGCGTGCCTGGGTCCCCCAGCTTCCTCAAT
GGCTCCACCGCCACCTCGCCCTTCGCCAAGGAGCGCCTTCGCCCCCGTGCTGCGCCCCCC
AAGCTCCCCACCCCAGGCCTGCCCCAGAGCCCACGGAGAGGGGCTTCCAGACCAGTCTTT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1442 Download

>KIAA1442  540 aa
MDALPRSGLNLKEEPLLPAGLGSVRSWMQGAGILDASTAAQSGVGLARAHFEKQPPSNLR
KSNFFHFVLAMYDRQGQPVEVERTAFIDFVEKDREPGAEKTNNGIHYRLRLVYNNGLRTE
QDLYVRLIDSMSKQAIIYEGQDKNPEMCRVLLTHEIMCSRCCDRKSCGNRNETPSDPVII
DRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVSVDGHVLAVSDNMFVHNNSKHGRR
ARRLDPSEAATPCIKAISPGEGWTTGGATVIVIGDNFFDGLQVVFGNVLVWSELITPHAI
RVQTPPRHIPGVVEVTLSYKSKQFCKGCPGRFVYTALNEPTIDYGFQRLQKVIPRHPGDP
ERLPKEVLLKRAADLAEALYGVPGSNQELLLKRAADVAEALYSTPRAPGPLAPLAPSHPH
SAVVGINAFSSPLAIAVGDATPGPEPGYARSCSSASPRGFAPSPGSQQSGYGGGLGAGLG
GYGAPGVAGLGVPGSPSFLNGSTATSPFAKERLRPRAAPPKLPTPGLPQSPRRGASRPVF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1442 Download

>pFN21ASDA1442  6349 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGACGCTCTGCCCCG
CAGCGGGCTGAACCTGAAGGAGGAGCCGCTGCTGCCCGCCGGCCTGGGCTCAGTGCGCTC
CTGGATGCAGGGCGCGGGCATCCTGGACGCCAGCACCGCGGCGCAGAGTGGCGTGGGTCT
GGCACGAGCACATTTTGAGAAGCAGCCTCCCTCCAACCTCAGGAAATCCAACTTCTTCCA
CTTCGTGCTGGCCATGTACGACCGGCAGGGGCAGCCCGTGGAGGTGGAGCGCACAGCCTT
CATCGACTTCGTGGAAAAGGACCGAGAGCCCGGGGCGGAAAAGACTAACAATGGGATCCA
TTACCGCCTCCGGCTGGTGTATAACAATGGACTGCGGACAGAGCAAGACCTCTACGTGCG
TCTCATCGACTCCATGTCCAAACAGGCCATCATCTATGAGGGGCAGGACAAGAACCCCGA
AATGTGCCGAGTGCTGCTCACCCATGAGATCATGTGCAGCCGGTGCTGTGACCGGAAGAG
CTGTGGCAACCGGAATGAGACGCCCTCAGACCCCGTCATCATTGACAGGTTCTTCCTCAA
GTTCTTCCTCAAATGCAACCAGAACTGCCTGAAGAATGCGGGGAATCCCAGAGACATGCG
CCGCTTCCAGGTGGTGGTGTCCACGACGGTGAGCGTGGACGGACACGTGCTGGCCGTGTC
CGACAACATGTTTGTGCACAACAACTCCAAGCATGGCCGCAGGGCGCGCCGCCTGGACCC
CTCCGAAGCTGCCACCCCCTGCATCAAGGCCATCAGCCCCGGGGAGGGCTGGACCACGGG
CGGCGCCACCGTCATTGTCATCGGCGACAACTTCTTCGACGGGTTGCAGGTCGTGTTCGG
AAACGTGCTCGTGTGGAGCGAGCTCATCACGCCCCACGCCATCCGGGTGCAGACGCCCCC
GCGGCACATCCCCGGGGTGGTGGAGGTGACCCTCTCCTACAAGTCCAAGCAGTTTTGCAA
GGGATGCCCCGGCCGCTTTGTCTACACAGCTCTGAACGAGCCCACCATTGACTACGGATT
CCAGAGGCTACAGAAAGTCATTCCCAGACACCCCGGAGACCCCGAGAGGCTGCCCAAGGA
AGTGCTGCTGAAGCGGGCGGCCGACTTGGCAGAAGCCCTGTACGGAGTGCCCGGCAGTAA
CCAGGAGCTGCTCCTGAAGCGCGCGGCGGACGTGGCCGAGGCTCTGTACAGCACCCCCCG
CGCACCCGGGCCGCTCGCACCCCTGGCCCCGAGCCACCCACACTCCGCCGTCGTGGGCAT
CAACGCCTTCAGCAGCCCGCTGGCCATCGCCGTCGGGGACGCCACCCCGGGGCCCGAGCC
GGGCTACGCGCGCAGCTGCAGCAGCGCGTCCCCCCGCGGGTTCGCGCCCAGCCCCGGCTC
GCAGCAGAGCGGCTACGGCGGCGGCCTCGGAGCTGGCCTGGGCGGCTACGGCGCGCCGGG
CGTGGCCGGCCTCGGCGTGCCTGGGTCCCCCAGCTTCCTCAATGGCTCCACCGCCACCTC
GCCCTTCGCCAAGGAGCGCCTTCGCCCCCGTGCTGCGCCCCCCAAGCTCCCCACCCCAGG
CCTGCCCCAGAGCCCACGGAGAGGGGCTTCCAGACCAGTCTTTTACGTAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT